FastQCFastQC Report
Wed 25 May 2016
SRR2049375_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049375_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4286401
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109970.25655555791443685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100490.23443910170793636No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63120.1472564046154338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67200.056.4607541
GTATCAA124600.040.4123271
TCAACGC152750.032.7975234
ATCAACG152800.032.7867933
CAACGCA157050.031.869615
TATCAAC162150.030.9252012
AACGCAG163700.030.62696
GTGGTAT30300.026.223251
ACGCAGA191000.026.0769677
CGCAGAG193650.025.7204178
GTACATG119250.025.1144391
GCAGAGT212650.023.0021029
TACATGG122450.022.9896892
TGGTATC31400.022.8995462
ACATGGG125650.021.4691333
AGAGTAC195400.021.07323310-11
GAGTACT146950.021.06788812-13
CAGAGTA207250.020.299510-11
AGTACTT157400.019.28085712-13
GTACTTT162700.018.89112514-15
Produced by FastQC (version 0.11.5)