##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049375_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4286401 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.851289695014536 31.0 31.0 34.0 30.0 34.0 2 32.04397558697845 33.0 31.0 34.0 30.0 34.0 3 32.02726669763282 34.0 31.0 34.0 30.0 34.0 4 35.70607066394395 37.0 35.0 37.0 33.0 37.0 5 35.7927625063544 37.0 35.0 37.0 35.0 37.0 6 35.838080244942084 37.0 35.0 37.0 35.0 37.0 7 35.77527090909133 37.0 35.0 37.0 33.0 37.0 8 35.78189931366664 37.0 35.0 37.0 33.0 37.0 9 37.5216595460854 39.0 37.0 39.0 35.0 39.0 10-11 37.48255167913595 39.0 37.0 39.0 34.5 39.0 12-13 37.425231563729106 39.0 37.0 39.0 34.0 39.0 14-15 38.71741281322023 40.0 38.0 41.0 34.0 41.0 16-17 38.747056446655364 40.0 38.0 41.0 35.0 41.0 18-19 38.81382924742692 40.0 38.0 41.0 35.0 41.0 20-21 38.84550407206419 40.0 38.0 41.0 35.0 41.0 22-23 38.813485019250415 40.0 38.0 41.0 35.0 41.0 24-25 38.79566622441531 40.0 38.0 41.0 35.0 41.0 26-27 38.68230118927277 40.0 38.0 41.0 34.0 41.0 28-29 38.60532717307596 40.0 38.0 41.0 34.0 41.0 30-31 38.50763554319813 40.0 38.0 41.0 34.0 41.0 32-33 38.380772004299175 40.0 38.0 41.0 34.0 41.0 34-35 38.285596937850656 40.0 38.0 41.0 34.0 41.0 36-37 38.17930625249481 40.0 38.0 41.0 34.0 41.0 38-39 38.09609436914558 40.0 38.0 41.0 33.5 41.0 40-41 37.99268372231156 40.0 38.0 41.0 33.0 41.0 42-43 37.86597532522039 40.0 37.0 41.0 33.0 41.0 44-45 37.69550212404299 40.0 37.0 41.0 33.0 41.0 46-47 37.476879204722096 40.0 37.0 41.0 33.0 41.0 48-49 37.27883858743034 40.0 37.0 41.0 32.0 41.0 50-51 37.13966938697523 40.0 37.0 41.0 32.0 41.0 52-53 37.00719111441043 40.0 36.5 41.0 31.0 41.0 54-55 36.86909472538851 40.0 36.0 41.0 31.0 41.0 56-57 36.72808062054857 39.0 36.0 41.0 31.0 41.0 58-59 36.542886794772585 39.0 35.0 41.0 31.0 41.0 60-61 36.36934621842427 39.0 35.0 41.0 31.0 41.0 62-63 36.17235368319483 39.0 35.0 41.0 30.5 41.0 64-65 35.946376458945394 38.5 35.0 40.0 30.0 41.0 66-67 35.681306881927284 38.0 35.0 40.0 30.0 41.0 68-69 35.39996374580913 37.0 35.0 40.0 29.5 41.0 70-71 35.07042633202073 37.0 34.5 39.5 29.0 41.0 72-73 34.773289759870806 36.0 34.0 39.0 29.0 41.0 74-75 34.46675556020074 36.0 34.0 39.0 29.0 41.0 76-77 34.138736903056895 35.5 34.0 38.5 29.0 40.0 78-79 33.805876888326594 35.0 34.0 37.0 29.0 39.0 80-81 33.48922499318192 35.0 34.0 37.0 29.0 39.0 82-83 33.1887821508067 35.0 34.0 36.5 28.5 39.0 84-85 32.917592054499806 35.0 34.0 36.0 28.0 37.5 86-87 32.68287253105811 35.0 33.0 36.0 27.5 37.0 88-89 32.455260602076194 35.0 33.0 35.0 27.0 37.0 90-91 32.262383057488094 35.0 33.0 35.0 27.0 36.0 92-93 32.08192070690539 35.0 33.0 35.0 27.0 36.0 94-95 31.924596882092928 35.0 33.0 35.0 26.5 36.0 96-97 31.727899349594217 35.0 33.0 35.0 26.0 36.0 98-99 31.58124239892628 35.0 33.0 35.0 25.5 35.0 100 31.470971334693136 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1572082087180604 1101 2 0.18361528150718698 1101 3 0.3791451142622648 1101 4 0.19114239318439274 1101 5 0.17653512859800458 1101 6 0.1708677455440295 1101 7 0.06804965122752549 1101 8 0.10729312842379102 1101 9 0.24879997592844916 1101 10-11 0.21699621359626775 1101 12-13 0.14269217921282973 1101 14-15 0.2932205513765993 1101 16-17 0.19227249952099612 1101 18-19 0.27387247469498277 1101 20-21 0.3017365515011079 1101 22-23 0.24063892821323662 1101 24-25 0.23986093291757982 1101 26-27 0.31893542301051525 1101 28-29 0.2711549871221308 1101 30-31 0.2908861463680097 1101 32-33 0.36902088722759885 1101 34-35 0.3089123207160327 1101 36-37 0.362864497810655 1101 38-39 0.38323428508971347 1101 40-41 0.38274828418872886 1101 42-43 0.38485526951235727 1101 44-45 0.4207255734500208 1101 46-47 0.4371868204101901 1101 48-49 0.4590960209161281 1101 50-51 0.3503880234745438 1101 52-53 0.4097286589842355 1101 54-55 0.38456774496626167 1101 56-57 0.3555662082480353 1101 58-59 0.3700258404400927 1101 60-61 0.33299638352294636 1101 62-63 0.36383648759985476 1101 64-65 0.3467118288642581 1101 66-67 0.25117191764661584 1101 68-69 0.3681320075925427 1101 70-71 0.3509843363335392 1101 72-73 0.3273362938355575 1101 74-75 0.36851394341022825 1101 76-77 0.2933959572021436 1101 78-79 0.2782677715988413 1101 80-81 0.36986932522741256 1101 82-83 0.36244708832379047 1101 84-85 0.3784836603886603 1101 86-87 0.2921387965958573 1101 88-89 0.36762174348469756 1101 90-91 0.33301503263538024 1101 92-93 0.359477200436082 1101 94-95 0.32312045498999 1101 96-97 0.37694366960154113 1101 98-99 0.39000073235369115 1101 100 0.3397737833520118 1102 1 0.20217675134302482 1102 2 0.2045730749159489 1102 3 0.35557311510515177 1102 4 0.20179962891479875 1102 5 0.17370584772024245 1102 6 0.19075806871223477 1102 7 0.20693925676643943 1102 8 0.22566080620797635 1102 9 0.17951223969767938 1102 10-11 0.207845371763959 1102 12-13 0.20852168737101806 1102 14-15 0.2809764655420466 1102 16-17 0.3006088574735344 1102 18-19 0.3036576025422306 1102 20-21 0.26188474315273425 1102 22-23 0.24669569811222658 1102 24-25 0.27720991895573377 1102 26-27 0.353969887644908 1102 28-29 0.33925508292176687 1102 30-31 0.3040810976976829 1102 32-33 0.3008766440060171 1102 34-35 0.325971586960776 1102 36-37 0.367865041307347 1102 38-39 0.3232079996131958 1102 40-41 0.34657891299170274 1102 42-43 0.3969670199109032 1102 44-45 0.3754104615444547 1102 46-47 0.3106417474414158 1102 48-49 0.318897442981239 1102 50-51 0.4013678932650251 1102 52-53 0.3960080114780311 1102 54-55 0.32068846853529465 1102 56-57 0.34133042736347363 1102 58-59 0.34444597486940154 1102 60-61 0.3391388977039398 1102 62-63 0.35066832384683977 1102 64-65 0.29117260936824607 1102 66-67 0.30196024157491763 1102 68-69 0.38795537517284373 1102 70-71 0.3870773375650174 1102 72-73 0.41479279073094943 1102 74-75 0.41094575861173155 1102 76-77 0.40919636758889055 1102 78-79 0.4223064674952184 1102 80-81 0.35192494724000767 1102 82-83 0.35588771084249515 1102 84-85 0.33011971897733616 1102 86-87 0.34914734004340886 1102 88-89 0.29330411226496267 1102 90-91 0.28241653193845195 1102 92-93 0.36944057650401163 1102 94-95 0.3262420756624138 1102 96-97 0.35911938168236546 1102 98-99 0.3346325206874994 1102 100 0.39708058229535936 1103 1 0.05539305813534057 1103 2 0.10909454617635106 1103 3 0.24545698783400383 1103 4 0.14269968860422466 1103 5 0.11315314568533807 1103 6 0.08882033942801115 1103 7 0.1394946341720953 1103 8 0.10041568997931449 1103 9 0.07593729715093644 1103 10-11 0.1091827895185915 1103 12-13 0.06741430497611844 1103 14-15 0.11851014570328999 1103 16-17 0.14690267333428153 1103 18-19 0.16029732782472195 1103 20-21 0.12364047682159196 1103 22-23 0.18504310599685425 1103 24-25 0.16255699921242694 1103 26-27 0.11905201747140381 1103 28-29 0.16999171875421837 1103 30-31 0.224758933426493 1103 32-33 0.24440511238199036 1103 34-35 0.24119255980059506 1103 36-37 0.26641221782470126 1103 38-39 0.2316249545797433 1103 40-41 0.2506589151408889 1103 42-43 0.33521461089867444 1103 44-45 0.34983790780982105 1103 46-47 0.34181632186735555 1103 48-49 0.2845658857830955 1103 50-51 0.28616609638589807 1103 52-53 0.29084479914332206 1103 54-55 0.2297955985444986 1103 56-57 0.18325296306981897 1103 58-59 0.23612750384913284 1103 60-61 0.24155819269351042 1103 62-63 0.26410769760092734 1103 64-65 0.3141140267568616 1103 66-67 0.24194023084749716 1103 68-69 0.23812169549537998 1103 70-71 0.34522845381076195 1103 72-73 0.30926402056260827 1103 74-75 0.2147418937550114 1103 76-77 0.24125450296705253 1103 78-79 0.30985518066268014 1103 80-81 0.3041487822695217 1103 82-83 0.21706870607589224 1103 84-85 0.22023744990639216 1103 86-87 0.2618025631664622 1103 88-89 0.31739094076795027 1103 90-91 0.2781024297022725 1103 92-93 0.2773146500390382 1103 94-95 0.24633855966819596 1103 96-97 0.36736602262120144 1103 98-99 0.3839560662866077 1103 100 0.40063158319713565 1104 1 0.21196104489470002 1104 2 0.15702748895721896 1104 3 0.28472049369749186 1104 4 0.2053692580230475 1104 5 0.17889094552556628 1104 6 0.2004194843282434 1104 7 0.1883931143963551 1104 8 0.16595236094287458 1104 9 0.20850742017427137 1104 10-11 0.17789390002231897 1104 12-13 0.1620028092576078 1104 14-15 0.24502092709243328 1104 16-17 0.18865243811450938 1104 18-19 0.18128276425209577 1104 20-21 0.22118874948500888 1104 22-23 0.20185405366640907 1104 24-25 0.2639544240299898 1104 26-27 0.25847161360343307 1104 28-29 0.22272464525463675 1104 30-31 0.2544833749819233 1104 32-33 0.28143003206165673 1104 34-35 0.1841214735670107 1104 36-37 0.21077886635906395 1104 38-39 0.1837965869312086 1104 40-41 0.17372301009115176 1104 42-43 0.16491893461495977 1104 44-45 0.15505107718598055 1104 46-47 0.2361694429745711 1104 48-49 0.19499896844310882 1104 50-51 0.13053968193543852 1104 52-53 0.0986219160643671 1104 54-55 0.15671694130899994 1104 56-57 0.12391850852469588 1104 58-59 0.2048956850429775 1104 60-61 0.18270831415812694 1104 62-63 0.1998531365753422 1104 64-65 0.12306917363095238 1104 66-67 0.10752875364750025 1104 68-69 0.14464653015822648 1104 70-71 0.23880028182468038 1104 72-73 0.26115549851127184 1104 74-75 0.24442303466970117 1104 76-77 0.17794285114837294 1104 78-79 0.19342186211444812 1104 80-81 0.19353641663643373 1104 82-83 0.18050345649139388 1104 84-85 0.2274055008566691 1104 86-87 0.18850297115802306 1104 88-89 0.17063687464220578 1104 90-91 0.13384890749595257 1104 92-93 0.19121844369011143 1104 94-95 0.20337244052561942 1104 96-97 0.2065495225666858 1104 98-99 0.19221104997001603 1104 100 0.21352216166101456 1105 1 0.1434137031333549 1105 2 0.13600151958168283 1105 3 0.17399064302554734 1105 4 0.14028375662272197 1105 5 0.14703576315182687 1105 6 0.10352447402444653 1105 7 0.07896877560639126 1105 8 0.08115607742587372 1105 9 0.09388019489687593 1105 10-11 0.18368655764439978 1105 12-13 0.1584729329743766 1105 14-15 0.20424044669100283 1105 16-17 0.20795709154393194 1105 18-19 0.14740286698183525 1105 20-21 0.17530716376662525 1105 22-23 0.18476864237988622 1105 24-25 0.16401842854516246 1105 26-27 0.19011607196019042 1105 28-29 0.169489488539611 1105 30-31 0.14218120442495064 1105 32-33 0.15945364662120198 1105 34-35 0.22478134375570846 1105 36-37 0.21640827571050636 1105 38-39 0.18341265469679513 1105 40-41 0.23495002810679466 1105 42-43 0.22508396144752396 1105 44-45 0.24359476696684368 1105 46-47 0.28879018954371816 1105 48-49 0.20014735979312803 1105 50-51 0.2653406371417688 1105 52-53 0.23182735893814055 1105 54-55 0.25903727691178347 1105 56-57 0.23859956494305834 1105 58-59 0.26158324388373444 1105 60-61 0.26977859581119645 1105 62-63 0.30110159219074006 1105 64-65 0.22930364974961037 1105 66-67 0.2926103659257393 1105 68-69 0.30971087852871904 1105 70-71 0.3114908428779586 1105 72-73 0.3242524253934036 1105 74-75 0.34040384042939564 1105 76-77 0.2998155582856228 1105 78-79 0.2902322365847354 1105 80-81 0.316100201795642 1105 82-83 0.3414977200363296 1105 84-85 0.3257325380807785 1105 86-87 0.3014328317899171 1105 88-89 0.2609586757800102 1105 90-91 0.2684055831502832 1105 92-93 0.326373190434758 1105 94-95 0.3128718109147073 1105 96-97 0.38847670112719257 1105 98-99 0.4074308209386679 1105 100 0.38713271351321055 1106 1 0.1605562887243508 1106 2 0.16598239504501322 1106 3 0.2554735016453833 1106 4 0.16965783951426516 1106 5 0.12363790092730653 1106 6 0.15438836991278748 1106 7 0.13818385825916124 1106 8 0.13245392643707277 1106 9 0.16987618917573855 1106 10-11 0.1532683445947498 1106 12-13 0.1322374763152112 1106 14-15 0.1848654597304673 1106 16-17 0.2001187668997062 1106 18-19 0.23166131017500646 1106 20-21 0.22084493414701 1106 22-23 0.18125218484679095 1106 24-25 0.1666756264412328 1106 26-27 0.22696015251041501 1106 28-29 0.2290066925091736 1106 30-31 0.21674135167229025 1106 32-33 0.1772915076402981 1106 34-35 0.15561359722883594 1106 36-37 0.1964372290999563 1106 38-39 0.16932841864669257 1106 40-41 0.16480999013590036 1106 42-43 0.21743018278679926 1106 44-45 0.22767028686447333 1106 46-47 0.29012663534710015 1106 48-49 0.30962909644014047 1106 50-51 0.2780224998948171 1106 52-53 0.28090163863854656 1106 54-55 0.2976725036128727 1106 56-57 0.3030428091135491 1106 58-59 0.33478283682086385 1106 60-61 0.252328302220576 1106 62-63 0.23815713216333734 1106 64-65 0.21256325895998174 1106 66-67 0.2656375598578151 1106 68-69 0.3103245095712026 1106 70-71 0.3125338371545112 1106 72-73 0.319632030060248 1106 74-75 0.3408475296800191 1106 76-77 0.3039398283658059 1106 78-79 0.31676517830936746 1106 80-81 0.2975196133162612 1106 82-83 0.3302716865579569 1106 84-85 0.32892526936597477 1106 86-87 0.33949724322939545 1106 88-89 0.38353972324827623 1106 90-91 0.3331640692287863 1106 92-93 0.4104077026895254 1106 94-95 0.4213845246748704 1106 96-97 0.44834245847042453 1106 98-99 0.4648077458522586 1106 100 0.4380108890555938 1107 1 0.12625336111745256 1107 2 0.08778303004878296 1107 3 0.16082827944398304 1107 4 0.1256955474846535 1107 5 0.0839616768960667 1107 6 0.045295177595761515 1107 7 0.030535729654737054 1107 8 0.08790384356143477 1107 9 0.06815652825351037 1107 10-11 0.08805062970107258 1107 12-13 0.12775171306722655 1107 14-15 0.12630750903750965 1107 16-17 0.07633197737245467 1107 18-19 0.05129950355039625 1107 20-21 0.07782098753973798 1107 22-23 0.05806222436732611 1107 24-25 0.04175214342418343 1107 26-27 0.14715129235553803 1107 28-29 0.1661212667724854 1107 30-31 0.15016256109720416 1107 32-33 0.00936689349172326 1107 34-35 0.12514121453135374 1107 36-37 0.07658837154004772 1107 38-39 0.12633820630115622 1107 40-41 0.11138418798238803 1107 42-43 0.18289074606941824 1107 44-45 0.11951418396747471 1107 46-47 0.15818752460677388 1107 48-49 0.10273616714960809 1107 50-51 0.11420394194320949 1107 52-53 0.08039887755339237 1107 54-55 0.02609814506192265 1107 56-57 0.008124393872080304 1107 58-59 0.07817013281566432 1107 60-61 0.10016789618214972 1107 62-63 0.05119001849813998 1107 64-65 0.05922993823985934 1107 66-67 0.10368368664529726 1107 68-69 -0.01848019839048476 1107 70-71 0.019100547768346132 1107 72-73 0.10939595770121002 1107 74-75 0.028102372731474645 1107 76-77 0.03132497105747234 1107 78-79 0.06217088825545147 1107 80-81 0.06349480018137399 1107 82-83 0.046352067605809566 1107 84-85 0.044062007442711604 1107 86-87 0.058909335673170915 1107 88-89 0.08939778413662225 1107 90-91 0.0547486923503584 1107 92-93 0.04699444369085626 1107 94-95 0.02901296983107926 1107 96-97 0.08430199613939848 1107 98-99 0.0625279512093222 1107 100 0.06850057084843186 1108 1 0.0788889694139634 1108 2 0.046458651358314285 1108 3 0.1795459856764552 1108 4 0.05109318312262445 1108 5 0.05826993059967123 1108 6 0.02110940098731362 1108 7 0.008365810520331252 1108 8 -0.025281677223070176 1108 9 0.13396023684546776 1108 10-11 0.09987124388478463 1108 12-13 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-0.23007479345911008 2215 6 -0.21691730046528335 2215 7 -0.1758206803486857 2215 8 -0.2294772389767843 2215 9 -0.3998524911988852 2215 10-11 -0.3344311489212899 2215 12-13 -0.35584317506797447 2215 14-15 -0.3879994817566512 2215 16-17 -0.39669529985246754 2215 18-19 -0.42342748236383443 2215 20-21 -0.42270438640396435 2215 22-23 -0.34891599720262434 2215 24-25 -0.34727402266122454 2215 26-27 -0.3859843463084687 2215 28-29 -0.324066069597194 2215 30-31 -0.43129570526556193 2215 32-33 -0.29408025362578627 2215 34-35 -0.3653545697985905 2215 36-37 -0.3876129640162489 2215 38-39 -0.4102963671712345 2215 40-41 -0.4336295163277768 2215 42-43 -0.424896006852336 2215 44-45 -0.37114020107430434 2215 46-47 -0.37933109675120136 2215 48-49 -0.38764055761755145 2215 50-51 -0.42810495255790926 2215 52-53 -0.39271930900976315 2215 54-55 -0.31222416053429924 2215 56-57 -0.36598168593471314 2215 58-59 -0.3589159013107164 2215 60-61 -0.33636357540546413 2215 62-63 -0.30361341806798947 2215 64-65 -0.27530069513354505 2215 66-67 -0.34650918963872357 2215 68-69 -0.4006970733276276 2215 70-71 -0.41253099875017085 2215 72-73 -0.4044172775313086 2215 74-75 -0.3561381305758502 2215 76-77 -0.19998732429398558 2215 78-79 -0.2828395618015378 2215 80-81 -0.33702118481617305 2215 82-83 -0.2962100916931405 2215 84-85 -0.34540663946851424 2215 86-87 -0.2673602503569512 2215 88-89 -0.3290589588742989 2215 90-91 -0.3480328924975211 2215 92-93 -0.31856605448525244 2215 94-95 -0.3900018246435195 2215 96-97 -0.37463475939177826 2215 98-99 -0.285626362859837 2215 100 -0.2768564353665681 2216 1 -0.34995063496829815 2216 2 -0.35619468714528324 2216 3 -0.45983598686304816 2216 4 -0.21399554288333889 2216 5 -0.4267504536249618 2216 6 -0.38112431678958814 2216 7 -0.5866069508674059 2216 8 -0.29553197183963675 2216 9 -0.5182475875137982 2216 10-11 -0.3963292654787338 2216 12-13 -0.37456147162716036 2216 14-15 -0.6371078330789217 2216 16-17 -0.44280423588780593 2216 18-19 -0.45866465926530964 2216 20-21 -0.49805909876891974 2216 22-23 -0.5058633203288565 2216 24-25 -0.42199083012013716 2216 26-27 -0.5920131003194129 2216 28-29 -0.6310575852517317 2216 30-31 -0.698009635269706 2216 32-33 -0.645042579350104 2216 34-35 -0.6012024696169718 2216 36-37 -0.6752323041855774 2216 38-39 -0.667403091448989 2216 40-41 -0.6201696075337111 2216 42-43 -0.6994320434277057 2216 44-45 -0.8237620230774283 2216 46-47 -0.7612289478822447 2216 48-49 -0.85107593868657 2216 50-51 -0.8212356880448013 2216 52-53 -0.631108792621724 2216 54-55 -0.6131520873835541 2216 56-57 -0.7532861664487314 2216 58-59 -0.7308747927660377 2216 60-61 -0.6105641672482349 2216 62-63 -0.5095224589564396 2216 64-65 -0.6388133985964544 2216 66-67 -0.5264151221320787 2216 68-69 -0.5675478722998619 2216 70-71 -0.6420502224031068 2216 72-73 -0.5877365839507931 2216 74-75 -0.5852884212080838 2216 76-77 -0.5588607519331248 2216 78-79 -0.7243642737751372 2216 80-81 -0.9040769530357551 2216 82-83 -0.825548060107792 2216 84-85 -0.8633776250684448 2216 86-87 -0.7256041814886842 2216 88-89 -1.064640143900533 2216 90-91 -0.8529932664658944 2216 92-93 -0.9070758227583084 2216 94-95 -0.8635280009184285 2216 96-97 -0.8591588110929713 2216 98-99 -1.0292062308286027 2216 100 -0.9976114436916568 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 232.0 3 19.0 4 42.0 5 66.0 6 229.0 7 568.0 8 1440.0 9 2405.0 10 3285.0 11 4402.0 12 6057.0 13 8520.0 14 10341.0 15 13300.0 16 16588.0 17 22869.0 18 22985.0 19 13218.0 20 10053.0 21 10021.0 22 10785.0 23 12052.0 24 14282.0 25 17573.0 26 22604.0 27 29972.0 28 39121.0 29 51320.0 30 67026.0 31 86914.0 32 112400.0 33 147397.0 34 200852.0 35 290118.0 36 467984.0 37 852269.0 38 1217124.0 39 498184.0 40 1784.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.41726524175572 20.552334448681943 14.583013887128773 23.44738642243356 2 15.184696905399193 20.544391437012077 40.15870190399825 24.112209753590484 3 17.706789448770657 25.540517557736663 30.903688199027574 25.849004794465102 4 12.358581727595059 17.341343477670527 38.50351740552324 31.796557389211173 5 12.465026020663956 38.021594339866944 35.93322230001346 13.580157339455642 6 30.452867102261315 38.65863226515671 17.182946719170697 13.705553913411276 7 26.303185352933617 33.207835664465364 22.50041468355387 17.988564299047148 8 24.907048193094326 36.66284757630074 20.100920104489568 18.32918412611537 9 24.691600249253394 16.474240277566192 21.553349768255465 37.28080970492495 10-11 23.711174012884 27.196347238627467 28.814627936117038 20.277850812371497 12-13 24.249599460010593 24.641614590730345 29.3007386927173 21.808047256541766 14-15 21.445433750272667 26.680508912501068 26.836187518882415 25.037869818343854 16-17 20.591458895236354 30.104089654700992 27.573038546790187 21.731412903272464 18-19 20.503373518963876 28.47094630353517 30.44912169292666 20.576558484574292 20-21 21.44059201229004 27.626742503465906 30.386635863799377 20.546029620444674 22-23 21.144871886694688 27.41708020318211 30.516370260271962 20.92167764985124 24-25 20.992740175697616 27.637899276047246 30.32807145672527 21.041289091529872 26-27 20.811009806137882 27.62837879925728 30.399843971522007 21.160767423082834 28-29 20.891699283476136 27.585398805381406 30.558554064971705 20.96434784617075 30-31 21.119447555057857 27.85092385218365 30.17122760358343 20.858400989175067 32-33 20.849702471389417 27.70272311612942 30.43326973287223 21.014304679608937 34-35 21.017985264084203 27.52338910199193 30.427202513625662 21.03142312029821 36-37 21.222399319895167 27.743772276862767 29.986937750530867 21.046890652711202 38-39 21.100475773153246 27.900052993292846 30.02205934758876 20.977411885965154 40-41 21.040874067325767 27.69963588304631 30.092356110230096 21.16713393939783 42-43 21.000090635889503 27.757795240577632 29.977697972054052 21.26441615147882 44-45 21.17150713898609 27.880411338857634 29.70055475547856 21.24752676667772 46-47 21.277083929210495 28.017626921251832 29.356676631521385 21.348612518016292 48-49 21.35714242387622 27.992599339208386 29.194465998533957 21.455792238381438 50-51 21.398634467994963 28.07313523647416 28.977031477318434 21.551198818212445 52-53 21.350661076093886 28.17000116065237 28.93873796843842 21.540599794815325 54-55 21.424472072990124 28.42674298266979 28.79278544079537 21.35599950354471 56-57 21.345182792768917 28.124673384372468 28.8761226245712 21.654021198287417 58-59 21.287151407283815 27.848351837808714 29.159745058522944 21.704751696384527 60-61 21.427358287642246 28.109641810808807 28.91597226013288 21.547027641416072 62-63 21.19709329534622 28.223884754591648 28.964313826677422 21.614708123384705 64-65 21.28304665226525 27.99319052996541 29.041545162722844 21.6822176550465 66-67 21.187591948946725 28.053463252849863 28.956621793947573 21.80232300425584 68-69 21.480870410774312 28.150237125158412 28.839729149130356 21.529163314936923 70-71 21.505508545603284 28.03662899662198 28.81073109266537 21.647131365109367 72-73 21.33385825393909 28.16799792364738 28.84135441181188 21.656789410601647 74-75 21.393110154382526 28.248404359310424 28.662484724822846 21.696000761484203 76-77 21.451192121076737 28.236719977090186 28.56107349512904 21.751014406704037 78-79 21.48710536875799 28.300810959619998 28.608723648478403 21.603360023143612 80-81 21.41962598027482 28.198634346002077 28.617620613405787 21.76411906031732 82-83 21.33407708984966 28.240665890714915 28.732576372105957 21.692680647329464 84-85 21.380302118835306 28.173863968951263 28.580474015603457 21.865359896609974 86-87 21.44671377132296 28.302839577429893 28.49206852836288 21.758378122884267 88-89 21.32653683754609 28.2131214222715 28.56463454195568 21.89570719822673 90-91 21.238029002656607 28.13034187784934 28.63871775567582 21.992911363818234 92-93 21.346809288475075 28.3296366727395 28.44351108333269 21.880042955452733 94-95 21.241645099675544 28.09061612497804 28.60233299123221 22.065405784114205 96-97 21.286104317790905 28.136197434720454 28.57620627689178 22.001491970596867 98-99 21.21427961508796 28.220054788793448 28.526777777829622 22.03888781828897 100 21.254435307673695 28.08715961368467 28.534720623151248 22.12368445549038 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 541.0 1 426.0 2 527.0 3 730.0 4 470.0 5 306.5 6 865.0 7 2406.5 8 4083.0 9 4575.0 10 3395.0 11 2086.5 12 1960.0 13 2214.5 14 2565.5 15 2969.0 16 3399.5 17 3917.0 18 4546.0 19 5308.5 20 6343.0 21 8079.0 22 10490.0 23 13847.5 24 18924.5 25 25469.5 26 33752.0 27 43958.5 28 55498.0 29 68518.5 30 81568.0 31 92787.5 32 102636.0 33 112251.0 34 121493.5 35 128704.0 36 134690.0 37 142073.0 38 148912.5 39 152269.5 40 153903.0 41 155854.5 42 156548.0 43 155456.5 44 152714.5 45 149113.5 46 145825.5 47 141003.5 48 134957.5 49 130091.5 50 126899.0 51 122465.0 52 113757.0 53 105757.0 54 99953.0 55 93946.5 56 86963.0 57 78746.0 58 71422.0 59 65029.0 60 57098.0 61 47840.5 62 40431.0 63 33843.0 64 27415.5 65 22316.0 66 17916.0 67 13678.0 68 10253.5 69 7729.5 70 5616.0 71 3980.5 72 2886.5 73 2013.0 74 1356.5 75 896.5 76 582.5 77 367.5 78 211.5 79 122.0 80 72.5 81 36.0 82 19.5 83 11.5 84 6.5 85 3.5 86 1.0 87 0.5 88 1.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03830719524374878 2 0.0 3 0.0 4 6.998878546360922E-5 5 0.0 6 0.0 7 0.0 8 9.331838061814562E-4 9 0.0 10-11 0.0 12-13 0.04368466692686942 14-15 1.3997757092721843E-4 16-17 0.0 18-19 3.499439273180461E-5 20-21 8.165358304087742E-5 22-23 0.0 24-25 5.832398788634101E-5 26-27 9.331838061814562E-5 28-29 2.7995514185443686E-4 30-31 2.3329595154536405E-5 32-33 1.5164236850448665E-4 34-35 6.998878546360922E-5 36-37 6.998878546360922E-5 38-39 2.2163115396809583E-4 40-41 8.165358304087742E-5 42-43 4.549271055134599E-4 44-45 1.8663676123629124E-4 46-47 6.998878546360922E-5 48-49 6.298990691724829E-4 50-51 3.032847370089733E-4 52-53 4.315975103589235E-4 54-55 3.032847370089733E-4 56-57 3.966031176271189E-4 58-59 1.2831277334995021E-4 60-61 1.6330716608175484E-4 62-63 8.165358304087741E-4 64-65 4.082679152043871E-4 66-67 0.0012831277334995022 68-69 0.0013297869238085751 70-71 3.6160872489531426E-4 72-73 8.981894134496516E-4 74-75 8.165358304087741E-4 76-77 6.648934619042876E-4 78-79 0.002822881013698905 80-81 0.0024029483009172497 82-83 0.0012131389480358932 84-85 0.0025079314791126637 86-87 0.001376446114117648 88-89 0.0017380548390129621 90-91 0.0024262778960717863 92-93 0.0023329595154536406 94-95 0.0033594617022532423 96-97 0.0028812050015852462 98-99 0.002869540204007978 100 0.0013531165189631115 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4286401.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.9231439416555 #Duplication Level Percentage of deduplicated Percentage of total 1 77.49092648008336 48.75972721078529 2 13.764589034909335 17.32222434282666 3 4.033527556896346 7.614067051656686 4 1.693092447140567 4.2613879903182275 5 0.8618177299742461 2.711414053732014 6 0.5188902699081607 1.9590124286013397 7 0.33149796252118324 1.4601225808460152 8 0.23351824164595023 1.1754961545672336 9 0.1732041033539261 0.9808692053962021 >10 0.8390355449432838 9.298473566471761 >50 0.04277049961884242 1.8278330439412578 >100 0.01635122067294267 1.6225158745756383 >500 5.563631631091278E-4 0.24416993785753555 >1k 1.1127258436859202E-4 0.12418541501823684 >5k 3.7090861456078366E-5 0.14731410253593022 >10k+ 7.418172291215673E-5 0.49118704086995996 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10997 0.25655555791443685 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10049 0.23443910170793636 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6312 0.1472564046154338 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.3329595154536405E-5 0.0 0.0 0.0 0.0 2 2.3329595154536405E-5 0.0 0.0 0.0 0.0 3 2.3329595154536405E-5 0.0 0.0 0.0 0.0 4 2.3329595154536405E-5 0.0 0.0 2.3329595154536405E-5 0.0 5 2.3329595154536405E-5 0.0 0.0 2.3329595154536405E-5 0.0 6 2.3329595154536405E-5 0.0 0.0 2.3329595154536405E-5 0.0 7 2.3329595154536405E-5 0.0 0.0 2.3329595154536405E-5 0.0 8 2.3329595154536405E-5 0.0 0.0 2.3329595154536405E-5 0.0 9 2.3329595154536405E-5 0.0 0.0 4.665919030907281E-5 0.0 10-11 2.3329595154536405E-5 0.0 0.0 6.998878546360922E-5 0.0 12-13 2.3329595154536405E-5 0.0 0.0 1.1664797577268203E-4 0.0 14-15 2.3329595154536405E-5 0.0 0.0 1.2831277334995021E-4 0.0 16-17 2.3329595154536405E-5 0.0 0.0 1.6330716608175484E-4 0.0 18-19 2.3329595154536405E-5 0.0 0.0 1.6330716608175484E-4 0.0 20-21 2.3329595154536405E-5 0.0 0.0 2.0996635639082762E-4 0.0 22-23 2.3329595154536405E-5 0.0 0.0 3.266143321635096E-4 0.0 24-25 2.3329595154536405E-5 0.0 0.0 5.832398788634102E-4 0.0 26-27 2.3329595154536405E-5 0.0 0.0 8.63195020717847E-4 0.0 28-29 2.3329595154536405E-5 0.0 0.0 0.0024496074912263226 0.0 30-31 2.3329595154536405E-5 0.0 0.0 0.006893895368165507 0.0 32-33 2.3329595154536405E-5 0.0 0.0 0.016983945272502503 0.0 34-35 2.3329595154536405E-5 0.0 0.0 0.032533120443001017 0.0 36-37 2.3329595154536405E-5 0.0 0.0 0.056282648310319074 0.0 38-39 2.3329595154536405E-5 2.3329595154536405E-5 0.0 0.08897907591940185 0.0 40-41 2.3329595154536405E-5 2.3329595154536405E-5 0.0 0.12246870976373886 0.0 42-43 2.3329595154536405E-5 2.3329595154536405E-5 0.0 0.15493184142127625 0.0 44-45 2.3329595154536405E-5 2.3329595154536405E-5 0.0 0.19518705786042884 0.0 46-47 4.665919030907281E-5 2.3329595154536405E-5 0.0 0.24407422450675986 0.0 48-49 4.665919030907281E-5 2.3329595154536405E-5 0.0 0.29667079678266217 0.0 50-51 4.665919030907281E-5 2.3329595154536405E-5 0.0 0.34572127059507496 0.0 52-53 4.665919030907281E-5 2.3329595154536405E-5 0.0 0.3946900908244469 0.0 54-55 4.665919030907281E-5 2.3329595154536405E-5 0.0 0.44876809239266224 0.0 56-57 4.665919030907281E-5 6.998878546360922E-5 0.0 0.5115713625486742 0.0 58-59 6.998878546360922E-5 8.165358304087742E-5 0.0 0.5803236794690931 0.0 60-61 6.998878546360922E-5 9.331838061814562E-5 0.0 0.6496709010659525 0.0 62-63 6.998878546360922E-5 9.331838061814562E-5 0.0 0.7190647818531211 0.0 64-65 6.998878546360922E-5 9.331838061814562E-5 0.0 0.7918531187352746 0.0 66-67 6.998878546360922E-5 1.1664797577268203E-4 0.0 0.8763179179922738 0.0 68-69 6.998878546360922E-5 1.3997757092721843E-4 0.0 0.9692163658976377 0.0 70-71 6.998878546360922E-5 1.3997757092721843E-4 0.0 1.0698369097991531 0.0 72-73 6.998878546360922E-5 1.3997757092721843E-4 0.0 1.1700725153806188 0.0 74-75 6.998878546360922E-5 1.3997757092721843E-4 0.0 1.2730726779878971 0.0 76-77 6.998878546360922E-5 1.3997757092721843E-4 0.0 1.3832466911051953 0.0 78-79 6.998878546360922E-5 1.3997757092721843E-4 0.0 1.507150170970938 0.0 80-81 6.998878546360922E-5 1.3997757092721843E-4 0.0 1.638880730011028 0.0 82-83 6.998878546360922E-5 1.3997757092721843E-4 0.0 1.7734574996599712 0.0 84-85 6.998878546360922E-5 1.3997757092721843E-4 0.0 1.909060771495714 0.0 86-87 6.998878546360922E-5 1.3997757092721843E-4 0.0 2.0535642838829125 0.0 88 6.998878546360922E-5 1.6330716608175484E-4 0.0 2.1718686609115667 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6720 0.0 56.460754 1 GTATCAA 12460 0.0 40.412327 1 TCAACGC 15275 0.0 32.797523 4 ATCAACG 15280 0.0 32.786793 3 CAACGCA 15705 0.0 31.86961 5 TATCAAC 16215 0.0 30.925201 2 AACGCAG 16370 0.0 30.6269 6 GTGGTAT 3030 0.0 26.22325 1 ACGCAGA 19100 0.0 26.076967 7 CGCAGAG 19365 0.0 25.720417 8 GTACATG 11925 0.0 25.114439 1 GCAGAGT 21265 0.0 23.002102 9 TACATGG 12245 0.0 22.989689 2 TGGTATC 3140 0.0 22.899546 2 ACATGGG 12565 0.0 21.469133 3 AGAGTAC 19540 0.0 21.073233 10-11 GAGTACT 14695 0.0 21.067888 12-13 CAGAGTA 20725 0.0 20.2995 10-11 AGTACTT 15740 0.0 19.280857 12-13 GTACTTT 16270 0.0 18.891125 14-15 >>END_MODULE