FastQCFastQC Report
Wed 25 May 2016
SRR2049374_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049374_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3662587
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24150.036.595341
GTACATG87500.028.4205651
GTATCAA34850.027.7875021
TACATGG85050.027.4690322
TCAACGC34050.026.644134
GAGTACA62500.026.6261461
ATCAACG35150.025.8103163
CAACGCA36000.025.0703325
ACATGGG88200.024.942423
AGTACAT63300.024.2831692
GCCGTAT8400.023.49926494
AACGCAG39500.023.083456
CATGGGG55500.023.0376014
GCCGGAT12150.022.43550994
ACGCAGA50150.021.9346121
CGCAGAG50400.021.731352
TATCAAC44350.021.198142
CCGTGCA8350.020.8262629
GCCGTGC7450.020.8187088
AGGCCGT7600.020.4078126
Produced by FastQC (version 0.11.5)