FastQCFastQC Report
Wed 25 May 2016
SRR2049374_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049374_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3662587
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT58430.15953204661076992No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT40600.11085060914593974RNA PCR Primer, Index 46 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30600.044.249741
GTATCAA51450.035.729841
TCAACGC60750.029.3967344
GTACATG87100.029.148441
ATCAACG61450.029.1383423
TACATGG85250.028.1700972
CAACGCA63500.028.1236485
TATCAAC67450.026.6857012
AACGCAG68950.025.9183676
GAGTACA64400.025.7708361
ACATGGG91650.025.7414513
AGTACAT66550.022.5270542
CATGGGG57950.021.8152714
ACGCAGA83450.021.1894597
CGCAGAG84150.020.8455358
ATGGGGG36200.019.2138735
TACACCG4950.018.9883215
CCGTGCA8850.018.067339
GCAGAGT95900.017.8501199
AGTATCG2152.626755E-517.486925
Produced by FastQC (version 0.11.5)