##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049374_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3662587
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.609065941641795	31.0	31.0	34.0	30.0	34.0
2	31.821560825722365	33.0	31.0	34.0	30.0	34.0
3	31.79159211781181	33.0	31.0	34.0	30.0	34.0
4	35.52933759662228	37.0	35.0	37.0	33.0	37.0
5	35.6663486764956	37.0	35.0	37.0	33.0	37.0
6	35.734923429805214	37.0	35.0	37.0	33.0	37.0
7	35.68490113681941	37.0	35.0	37.0	33.0	37.0
8	35.67328557656105	37.0	35.0	37.0	33.0	37.0
9	37.416007592447635	39.0	37.0	39.0	34.0	39.0
10-11	37.38649989747684	39.0	37.0	39.0	34.0	39.0
12-13	37.330285942695696	39.0	37.0	39.0	34.0	39.0
14-15	38.603602180644444	40.0	38.0	41.0	34.0	41.0
16-17	38.625499953994265	40.0	38.0	41.0	34.0	41.0
18-19	38.67156097588945	40.0	38.0	41.0	34.0	41.0
20-21	38.69685593816611	40.0	38.0	41.0	34.0	41.0
22-23	38.65477611862872	40.0	38.0	41.0	34.0	41.0
24-25	38.63702049944479	40.0	38.0	41.0	34.0	41.0
26-27	38.525798295030256	40.0	38.0	41.0	34.0	41.0
28-29	38.44858401998369	40.0	38.0	41.0	34.0	41.0
30-31	38.38420002036812	40.0	38.0	41.0	34.0	41.0
32-33	38.280643026363606	40.0	38.0	41.0	34.0	41.0
34-35	38.19568367932284	40.0	38.0	41.0	34.0	41.0
36-37	38.09656507818108	40.0	38.0	41.0	33.0	41.0
38-39	38.02574164108593	40.0	38.0	41.0	33.0	41.0
40-41	37.94996391894581	40.0	37.5	41.0	33.0	41.0
42-43	37.858990926358885	40.0	37.0	41.0	33.0	41.0
44-45	37.75245775731744	40.0	37.0	41.0	33.0	41.0
46-47	37.615062659262435	40.0	37.0	41.0	33.0	41.0
48-49	37.493022964369175	40.0	37.0	41.0	32.5	41.0
50-51	37.37769136951559	40.0	37.0	41.0	32.0	41.0
52-53	37.256532336296715	40.0	36.5	41.0	32.0	41.0
54-55	37.12592260607052	39.5	36.0	41.0	31.5	41.0
56-57	36.98438985886206	39.0	36.0	41.0	31.0	41.0
58-59	36.79115990964856	39.0	35.5	41.0	31.0	41.0
60-61	36.60132428253581	39.0	35.0	41.0	31.0	41.0
62-63	36.395879060347234	39.0	35.0	41.0	31.0	41.0
64-65	36.133971152084584	38.0	35.0	40.0	30.5	41.0
66-67	35.82331819012081	37.5	35.0	40.0	30.0	41.0
68-69	35.47750538075955	37.0	35.0	40.0	30.0	41.0
70-71	35.09045491615626	36.5	34.0	39.0	29.0	41.0
72-73	34.75368830829138	36.0	34.0	39.0	29.0	41.0
74-75	34.39331748297037	35.5	34.0	39.0	29.0	40.5
76-77	34.00259256640183	35.0	34.0	37.5	29.0	39.5
78-79	33.60120032643593	35.0	33.5	37.0	28.0	39.0
80-81	33.22882951312829	35.0	33.0	36.5	27.5	39.0
82-83	32.89745431303065	35.0	33.0	36.0	27.0	38.0
84-85	32.61220962123221	35.0	33.0	36.0	27.0	37.0
86-87	32.354217524389185	35.0	33.0	35.0	26.5	37.0
88-89	32.07366227751041	35.0	33.0	35.0	26.0	36.0
90-91	31.835840076972914	35.0	33.0	35.0	25.5	36.0
92-93	31.623001037244986	35.0	33.0	35.0	25.0	36.0
94-95	31.43448292149784	35.0	32.5	35.0	25.0	35.5
96-97	31.19966515471168	35.0	32.0	35.0	24.0	35.0
98-99	30.992258067862963	35.0	32.0	35.0	23.5	35.0
100	30.8314732182471	35.0	32.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.215891390957772
1101	2	0.22821136384049723
1101	3	0.4245315843025992
1101	4	0.1821583889425611
1101	5	0.18312143409058024
1101	6	0.1764200134046945
1101	7	0.016700572036057793
1101	8	0.11210634125244923
1101	9	0.2635955323882868
1101	10-11	0.22876166106594553
1101	12-13	0.2119483173976704
1101	14-15	0.3696150908285176
1101	16-17	0.19081008858655224
1101	18-19	0.2633012815608424
1101	20-21	0.23018888596700293
1101	22-23	0.2345917987157904
1101	24-25	0.21944118217738406
1101	26-27	0.3031658089712437
1101	28-29	0.32050549230022085
1101	30-31	0.331852072423402
1101	32-33	0.28623497830488276
1101	34-35	0.2979056235013502
1101	36-37	0.3159288668998954
1101	38-39	0.34555035877496465
1101	40-41	0.3512887330445338
1101	42-43	0.4421608988448611
1101	44-45	0.39690983820584336
1101	46-47	0.40828618282370144
1101	48-49	0.4758014484599258
1101	50-51	0.40162135396439425
1101	52-53	0.451661133496458
1101	54-55	0.3687845825465601
1101	56-57	0.39561327541660063
1101	58-59	0.40383262663665676
1101	60-61	0.46229670492142816
1101	62-63	0.4496647064678143
1101	64-65	0.36225546353841054
1101	66-67	0.3924026633718043
1101	68-69	0.3996118209047026
1101	70-71	0.30256433298880836
1101	72-73	0.29867643817643597
1101	74-75	0.39228629688778227
1101	76-77	0.26364728283447647
1101	78-79	0.34411807923638094
1101	80-81	0.4323883518085694
1101	82-83	0.4301305268421487
1101	84-85	0.41060031132715125
1101	86-87	0.40532391851921545
1101	88-89	0.4356799084951106
1101	90-91	0.4419225474944426
1101	92-93	0.5280210022043619
1101	94-95	0.5149415469645717
1101	96-97	0.5545127469851039
1101	98-99	0.5498352324306843
1101	100	0.5803362792488862
1102	1	0.3514543028128969
1102	2	0.2838106701447707
1102	3	0.49726972698892524
1102	4	0.26749621299046566
1102	5	0.26573127726106094
1102	6	0.2588369852283847
1102	7	0.24477761799500541
1102	8	0.2904508960295118
1102	9	0.2465822493760328
1102	10-11	0.3392665245226425
1102	12-13	0.3615208482437282
1102	14-15	0.3511822533330431
1102	16-17	0.3729108261992451
1102	18-19	0.37057274504297055
1102	20-21	0.38311075330356914
1102	22-23	0.4176809351971045
1102	24-25	0.41403421295024145
1102	26-27	0.4519786083626016
1102	28-29	0.45138686627081626
1102	30-31	0.4057741566734592
1102	32-33	0.46213444798881653
1102	34-35	0.3647398193240008
1102	36-37	0.47437855526040806
1102	38-39	0.5192091015102278
1102	40-41	0.5309224805080888
1102	42-43	0.5616193203772113
1102	44-45	0.5652459194661219
1102	46-47	0.5737441534324574
1102	48-49	0.5300581351118439
1102	50-51	0.633774610284533
1102	52-53	0.6940069192308229
1102	54-55	0.6100313755929321
1102	56-57	0.6696065243851947
1102	58-59	0.642988555373627
1102	60-61	0.6432810860822258
1102	62-63	0.686501904885958
1102	64-65	0.7037600951220853
1102	66-67	0.6545498183324412
1102	68-69	0.6968399230613329
1102	70-71	0.7004362835111664
1102	72-73	0.7416663928383116
1102	74-75	0.7240421100088099
1102	76-77	0.6818901340393069
1102	78-79	0.7209374009917369
1102	80-81	0.7821776358551773
1102	82-83	0.8203188782676278
1102	84-85	0.8073198421956462
1102	86-87	0.8045229948738282
1102	88-89	0.8273196034485011
1102	90-91	0.8761812002587064
1102	92-93	0.9276432201286866
1102	94-95	0.8849908707394718
1102	96-97	0.8906805589478282
1102	98-99	0.9649103988289696
1102	100	0.9992597899989271
1103	1	0.14988018369115963
1103	2	0.1873153989083356
1103	3	0.33581333621481946
1103	4	0.17282531388593014
1103	5	0.2061032450460729
1103	6	0.1575445451928985
1103	7	0.16982855933860463
1103	8	0.1513474652280351
1103	9	0.21442395812316306
1103	10-11	0.17838795241434013
1103	12-13	0.222999598686485
1103	14-15	0.298772291328973
1103	16-17	0.2506669384430751
1103	18-19	0.2545327152642045
1103	20-21	0.25957141242442106
1103	22-23	0.26579819370473956
1103	24-25	0.2850983625526382
1103	26-27	0.3422017482386508
1103	28-29	0.3824436045310762
1103	30-31	0.3403540551064239
1103	32-33	0.33979733677889357
1103	34-35	0.34418930225569966
1103	36-37	0.3697424993911653
1103	38-39	0.36726811474850507
1103	40-41	0.38016309326693687
1103	42-43	0.45370250858210426
1103	44-45	0.5376321164696094
1103	46-47	0.4604539634792033
1103	48-49	0.42783885074688044
1103	50-51	0.4193919027233264
1103	52-53	0.5292375670074065
1103	54-55	0.458474237565369
1103	56-57	0.45907674914756313
1103	58-59	0.5269829996590829
1103	60-61	0.43327520804099606
1103	62-63	0.3761978862958628
1103	64-65	0.4230377514069019
1103	66-67	0.43974314570474604
1103	68-69	0.4355440053722148
1103	70-71	0.480473741191247
1103	72-73	0.489915428359474
1103	74-75	0.5207127219975192
1103	76-77	0.38278710343168854
1103	78-79	0.41103243229797926
1103	80-81	0.5522990497090632
1103	82-83	0.559543949389294
1103	84-85	0.5080701707850537
1103	86-87	0.5331732340478226
1103	88-89	0.5757273204891078
1103	90-91	0.6126397761998703
1103	92-93	0.6787539907647897
1103	94-95	0.596598900861963
1103	96-97	0.6727996645517749
1103	98-99	0.692345844107848
1103	100	0.8202672299595726
1104	1	0.24636798604341692
1104	2	0.2720451072371013
1104	3	0.39830354325000883
1104	4	0.2561179964876814
1104	5	0.26394121183250263
1104	6	0.25249012104770685
1104	7	0.2789571051495585
1104	8	0.27368602905895756
1104	9	0.26462276420944164
1104	10-11	0.2667562161645378
1104	12-13	0.2737186026816616
1104	14-15	0.3400020105575692
1104	16-17	0.3462025661026047
1104	18-19	0.3618902658075882
1104	20-21	0.3436755760123802
1104	22-23	0.314931280566654
1104	24-25	0.33339015363172564
1104	26-27	0.3734033071818885
1104	28-29	0.45237922173260614
1104	30-31	0.42472193267010994
1104	32-33	0.45741177986241865
1104	34-35	0.4204354868107174
1104	36-37	0.39718766782578996
1104	38-39	0.394774592483202
1104	40-41	0.39992628673437025
1104	42-43	0.4659409439921305
1104	44-45	0.43228068363767136
1104	46-47	0.44205606130159936
1104	48-49	0.372686224001221
1104	50-51	0.4228584159962452
1104	52-53	0.3662173613389825
1104	54-55	0.4175511013525721
1104	56-57	0.37639288724882647
1104	58-59	0.47232415195477984
1104	60-61	0.4615474495232519
1104	62-63	0.4914063318911275
1104	64-65	0.48475556715555257
1104	66-67	0.548390857171718
1104	68-69	0.5053084567940189
1104	70-71	0.5334077307933498
1104	72-73	0.554953440308104
1104	74-75	0.4969362159038866
1104	76-77	0.4645775073467817
1104	78-79	0.4767618261929272
1104	80-81	0.5047549837865546
1104	82-83	0.5789853146754709
1104	84-85	0.5810443046506109
1104	86-87	0.5558104972269646
1104	88-89	0.474884878123774
1104	90-91	0.4131254013599204
1104	92-93	0.5144945448511464
1104	94-95	0.5741043762498634
1104	96-97	0.6918812804487544
1104	98-99	0.6844427320821005
1104	100	0.6257691900861779
1105	1	0.21058621379085807
1105	2	0.19326301313337169
1105	3	0.3333650508178536
1105	4	0.1989767086117311
1105	5	0.16482869822410606
1105	6	0.16311542647031274
1105	7	0.1642920425746297
1105	8	0.16471292033813967
1105	9	0.22034348494402423
1105	10-11	0.24268273355122005
1105	12-13	0.22221687507855847
1105	14-15	0.2677498598130228
1105	16-17	0.24902579354515808
1105	18-19	0.23351530437176393
1105	20-21	0.2440202997855181
1105	22-23	0.2640820958665202
1105	24-25	0.30732289347437103
1105	26-27	0.3659018254045421
1105	28-29	0.34949399407890525
1105	30-31	0.31897102919717923
1105	32-33	0.33722191332137896
1105	34-35	0.3864738785151829
1105	36-37	0.4051226278293427
1105	38-39	0.362597307104771
1105	40-41	0.4038762566097702
1105	42-43	0.4315152147492327
1105	44-45	0.4205981803954799
1105	46-47	0.3843566210476723
1105	48-49	0.4119495189494131
1105	50-51	0.4412259229745601
1105	52-53	0.44276075842594054
1105	54-55	0.3662355214406148
1105	56-57	0.33964559568404695
1105	58-59	0.3572522525373216
1105	60-61	0.3853855987740218
1105	62-63	0.39039713302146595
1105	64-65	0.380223823331292
1105	66-67	0.4267241551086016
1105	68-69	0.4129418903694031
1105	70-71	0.39418237331232575
1105	72-73	0.4887456518107314
1105	74-75	0.4304425711734794
1105	76-77	0.39136206025528963
1105	78-79	0.44297360918788087
1105	80-81	0.5009843730083858
1105	82-83	0.45783642672068936
1105	84-85	0.5245013022254952
1105	86-87	0.4102699348311134
1105	88-89	0.4334834496873299
1105	90-91	0.5036128781483171
1105	92-93	0.5493545218082261
1105	94-95	0.5187326371218788
1105	96-97	0.5892180928253232
1105	98-99	0.6111431372055485
1105	100	0.6656308559838706
1106	1	0.11674322620148558
1106	2	0.12055314593519739
1106	3	0.24259082786711872
1106	4	0.1539601743518162
1106	5	0.11349649293713782
1106	6	0.12830033516182482
1106	7	0.1879204187397221
1106	8	0.11277972704736783
1106	9	0.20082716799096545
1106	10-11	0.15849916859939128
1106	12-13	0.11948386444746006
1106	14-15	0.18830561513290434
1106	16-17	0.19398602327728298
1106	18-19	0.16587330103104136
1106	20-21	0.19484367486465004
1106	22-23	0.19020043733782188
1106	24-25	0.15037321547087856
1106	26-27	0.2295304858141094
1106	28-29	0.23551148678381395
1106	30-31	0.2196003829742068
1106	32-33	0.1727044054426372
1106	34-35	0.16173422379985425
1106	36-37	0.15303086393362264
1106	38-39	0.20995238455373055
1106	40-41	0.195837496876635
1106	42-43	0.20128162283491946
1106	44-45	0.2094092780038892
1106	46-47	0.2981534860592916
1106	48-49	0.2403744358853288
1106	50-51	0.2592716137745299
1106	52-53	0.2884435526091096
1106	54-55	0.22354132321914477
1106	56-57	0.20673767071663463
1106	58-59	0.17915217781557402
1106	60-61	0.14489498629009745
1106	62-63	0.14852795113020534
1106	64-65	0.1311637777615573
1106	66-67	0.09678087720195805
1106	68-69	0.1505683323030027
1106	70-71	0.1884438098415302
1106	72-73	0.22954357103685652
1106	74-75	0.18488195973883848
1106	76-77	0.2096038862626557
1106	78-79	0.2194396628187505
1106	80-81	0.213010557922388
1106	82-83	0.28346639250344907
1106	84-85	0.24038279731362167
1106	86-87	0.22997689116375852
1106	88-89	0.20492179877194872
1106	90-91	0.1915863248899825
1106	92-93	0.3067199497329085
1106	94-95	0.2747228205172618
1106	96-97	0.29437196671406696
1106	98-99	0.32134895139369846
1106	100	0.3569747348524359
1107	1	0.2017814447382662
1107	2	0.14769150173889756
1107	3	0.3095168560687753
1107	4	0.17728200878028133
1107	5	0.1522262740103031
1107	6	0.15456250450080944
1107	7	0.12786933934561517
1107	8	0.15022146773113576
1107	9	0.1782522625079821
1107	10-11	0.14349999534086066
1107	12-13	0.1466182953010815
1107	14-15	0.22541445437380503
1107	16-17	0.19121453940628186
1107	18-19	0.20189456944417827
1107	20-21	0.16286774772498802
1107	22-23	0.18162956607802272
1107	24-25	0.1788286187651309
1107	26-27	0.23161782430359068
1107	28-29	0.24346402985278814
1107	30-31	0.2324597078533941
1107	32-33	0.28505835312459027
1107	34-35	0.24991742195488342
1107	36-37	0.23840565866733243
1107	38-39	0.2877295236366635
1107	40-41	0.3194048392779294
1107	42-43	0.3964251054052781
1107	44-45	0.3090475133225681
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2216	94-95	-1.701912614027055
2216	96-97	-1.8149812888832528
2216	98-99	-2.0157664429596878
2216	100	-2.029901712645554
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	114.0
3	15.0
4	27.0
5	44.0
6	110.0
7	325.0
8	727.0
9	1347.0
10	2172.0
11	3226.0
12	4567.0
13	6291.0
14	7091.0
15	8188.0
16	9649.0
17	12341.0
18	11878.0
19	8786.0
20	8802.0
21	9841.0
22	11774.0
23	14047.0
24	17611.0
25	21209.0
26	25182.0
27	29864.0
28	38691.0
29	49660.0
30	65427.0
31	83548.0
32	107488.0
33	138017.0
34	186705.0
35	263962.0
36	423541.0
37	765000.0
38	988975.0
39	334805.0
40	1540.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.359762502929385	21.468476573958903	12.198710689607891	23.973050233503827
2	17.242539221593915	22.52356053248701	35.98917923314859	24.24472101277048
3	19.089949262638676	25.328763521521807	30.160894471585248	25.420392744254265
4	13.204857554120261	16.659140104363477	37.4156606374079	32.72034170410836
5	12.974763466369534	38.19062864581783	35.64679828765842	13.187809600154207
6	31.745247756639706	37.64389423210813	16.816260423646025	13.794597587606134
7	27.15588735503075	31.993151288965972	22.41161779911303	18.439343556890254
8	25.679809532650573	34.86278760542461	20.999243700461697	18.458159161463126
9	24.85835831339979	14.94165189796174	21.22649919305671	38.97349059558176
10-11	24.179562151015116	26.3681244977935	28.96968727295761	20.482626078233775
12-13	24.422678836845034	23.772341929877296	29.06415156081267	22.740827672465
14-15	21.724581418870816	26.134954266546696	26.081344537998675	26.05911977658381
16-17	21.291234856673714	29.522124662158195	26.95075912189936	22.235881359268735
18-19	21.045608212339587	28.418431676416606	29.052956026152017	21.48300408509179
20-21	22.115159102109576	27.84291695619351	28.531051156491934	21.510872785204985
22-23	21.689901154566428	27.822915878858307	28.620658021229257	21.866524945346008
24-25	21.74679252871233	27.848322283067283	28.680590452389932	21.72429473583045
26-27	21.405103418091787	27.898701040208085	28.805342238520364	21.890853303179764
28-29	21.403295471501377	27.726093538378706	29.050217859228137	21.82039313089178
30-31	21.65634563765994	28.252816929672935	28.562597966956144	21.528239465710985
32-33	21.537419397643028	27.8372562066238	28.957232048783112	21.66809234695006
34-35	21.49617005475641	27.291352965187155	29.277818808300694	21.934658171755743
36-37	21.630067511070393	27.617219479850906	28.771472712779733	21.981240296298964
38-39	21.446971841926427	27.294036187128555	29.228905471645206	22.03008649929981
40-41	21.115013487064527	26.843142279213566	29.786160506647835	22.25568372707407
42-43	20.99185666440641	26.6628405689946	29.885472355374944	22.459830411224043
44-45	20.683699776591453	26.901269531183342	29.982382601300046	22.432648090925163
46-47	20.676006430979662	27.133620298349513	29.694776924539738	22.495596346131087
48-49	20.314133080832075	27.076159381590344	29.61056431628622	22.99914322129136
50-51	20.078212957068743	26.97809144385034	29.493555676630407	23.450139922450514
52-53	19.92239040289415	26.813453695329432	29.8268220678828	23.43733383389362
54-55	19.812672266567283	26.99042883880559	29.57804161837401	23.61885727625312
56-57	19.925740496098154	26.865473794774502	29.06495904573889	24.143826663388452
58-59	19.774541474242	26.651318653610478	29.015089224660045	24.559050647487474
60-61	20.0292826568175	26.9876200571865	28.218078661816715	24.765018624179284
62-63	19.65011521349137	26.94454421559196	28.15267838774012	25.25266218317655
64-65	19.628957110290223	26.755322118890064	28.030902357184583	25.58481841363513
66-67	19.828091440107563	27.069482189694906	27.59704122438911	25.505385145808425
68-69	20.33528180375811	27.324656091648098	27.142118387530374	25.19794371706342
70-71	20.220187068224682	27.243538856280463	26.907981900674866	25.62829217481999
72-73	20.125052405506608	27.38258879857979	26.856642970481314	25.635715825432293
74-75	20.55811160921621	27.202285731759897	26.61022507014927	25.62937758887463
76-77	20.789072324940467	27.314259393854673	26.27090276348144	25.625765517723426
78-79	20.722444568587072	27.490051752185828	26.30946354548071	25.478040133746386
80-81	20.504597150678528	27.343503838649486	26.412100960932	25.73979804973999
82-83	20.506132563521177	27.063073876220006	26.513209349198654	25.91758421106017
84-85	20.793506039623423	26.654848627159737	26.419721063323216	26.131924269893624
86-87	20.977521198064895	26.86032464632805	26.299402368220193	25.862751787386866
88-89	20.82020878324721	26.454096395460326	26.51218502956923	26.21350979172324
90-91	20.710246098473792	26.006019969545203	26.63948469847046	26.644249233510546
92-93	20.695879033116814	25.78585880097966	26.8758549511398	26.642407214763725
94-95	20.653735592953776	25.25298680334771	27.353919950721426	26.73935765297708
96-97	20.82107553533966	25.178674086806847	27.30243528640346	26.697815091450032
98-99	20.880953092804827	25.22836665300105	27.231478481841453	26.65920177235267
100	20.744095424460777	25.40888962062131	27.28564676818635	26.56136818673156
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	23.0
2	57.5
3	96.0
4	74.5
5	56.5
6	121.5
7	252.0
8	426.0
9	520.0
10	466.0
11	443.5
12	516.5
13	589.0
14	679.0
15	830.5
16	975.5
17	1170.5
18	1456.0
19	1892.0
20	2508.5
21	3491.5
22	4984.5
23	6983.5
24	9330.0
25	12215.5
26	16240.0
27	21292.5
28	27217.5
29	34069.0
30	40872.0
31	48060.5
32	55413.0
33	62700.5
34	71037.5
35	79571.0
36	87825.0
37	96630.0
38	105517.0
39	114000.0
40	123037.0
41	130974.5
42	137648.0
43	144922.5
44	150822.5
45	155075.5
46	158420.0
47	158768.5
48	156428.0
49	152893.0
50	148633.5
51	142120.0
52	134273.0
53	126947.0
54	119019.0
55	109930.0
56	97292.0
57	82353.0
58	68687.5
59	56738.0
60	46260.5
61	36585.0
62	28572.0
63	22459.0
64	17224.0
65	12935.5
66	9689.5
67	7055.0
68	5046.0
69	3675.0
70	2631.5
71	1835.0
72	1188.5
73	707.5
74	419.0
75	275.5
76	177.0
77	103.0
78	67.5
79	34.0
80	18.5
81	14.5
82	6.5
83	2.0
84	1.5
85	1.0
86	1.5
87	1.0
88	0.5
89	1.0
90	0.5
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03836086350986338
2	0.0
3	0.0
4	8.190931710291114E-5
5	0.0
6	2.730310570097038E-5
7	0.0
8	4.6415279691649645E-4
9	0.0
10-11	0.0
12-13	0.04263379955206525
14-15	1.5016708135533709E-4
16-17	0.0
18-19	2.730310570097038E-5
20-21	5.460621140194076E-5
22-23	0.0
24-25	1.5016708135533709E-4
26-27	6.825776425242596E-5
28-29	4.231981383650409E-4
30-31	0.0
32-33	2.1842484560776303E-4
34-35	5.460621140194076E-5
36-37	6.825776425242596E-5
38-39	3.8224347981358534E-4
40-41	9.556086995339634E-5
42-43	5.870167725708632E-4
44-45	3.958950326640705E-4
46-47	9.556086995339634E-5
48-49	8.463962767300818E-4
50-51	6.143198782718335E-4
52-53	6.689260896737743E-4
54-55	6.006683254213483E-4
56-57	6.962291953747446E-4
58-59	1.7747018705630745E-4
60-61	2.593795041592186E-4
62-63	0.0010511695694873596
64-65	5.05107455467952E-4
66-67	0.0015972316835067672
68-69	0.0015289739192543414
70-71	5.324105611689224E-4
72-73	0.0011603819922912412
74-75	0.0010921242280388154
76-77	9.010024881320225E-4
78-79	0.00363131305822906
80-81	0.0027576136757980086
82-83	0.0013242006264970634
84-85	0.0029214323100038306
86-87	0.001296897520796093
88-89	0.0021979000089281157
90-91	0.003003341627106742
92-93	0.002648401252994127
94-95	0.0039043441152387644
96-97	0.0033446304483688715
98-99	0.003016993179957227
100	0.0012559428622446376
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3662587.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.407684759791
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.7690362148172	60.65521966874
2	10.593211709255181	15.340598680749517
3	2.7718459653948955	6.021088465950364
4	1.0297227549172128	2.9823936251211625
5	0.5305626050712544	1.9208404926666443
6	0.3050079166147456	1.3250950245288673
7	0.205779042422397	1.0429988823724807
8	0.13830830535960897	0.8011667339311584
9	0.09805347576813873	0.6389842646719076
>10	0.5268925773723597	6.687430197197161
>50	0.024231049395714067	1.1865484355781049
>100	0.007009023491540374	0.8310806464141177
>500	1.1312003990959423E-4	0.066754695542798
>1k	1.8853339975682544E-4	0.3402714806508519
>5k	3.770667995136509E-5	0.15952870588485335
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT	5843	0.15953204661076992	No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	4060	0.11085060914593974	RNA PCR Primer, Index 46 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	2.730310570097038E-5	0.0
12-13	0.0	0.0	0.0	2.730310570097038E-5	0.0
14-15	0.0	0.0	0.0	5.460621140194076E-5	0.0
16-17	0.0	0.0	0.0	5.460621140194076E-5	0.0
18-19	0.0	0.0	0.0	1.6381863420582228E-4	0.0
20-21	0.0	0.0	0.0	7.91790065328141E-4	0.0
22-23	1.365155285048519E-5	0.0	0.0	0.0024299764073863637	0.0
24-25	2.730310570097038E-5	0.0	0.0	0.005255847847436798	0.0
26-27	2.730310570097038E-5	0.0	0.0	0.011248879548799797	0.0
28-29	2.730310570097038E-5	0.0	0.0	0.04838110330211952	0.0
30-31	2.730310570097038E-5	0.0	0.0	0.2204862300881863	0.0
32-33	2.730310570097038E-5	0.0	0.0	0.5708533339958887	0.0
34-35	2.730310570097038E-5	0.0	0.0	1.190483666326561	0.0
36-37	2.730310570097038E-5	0.0	0.0	2.164207976493118	0.0
38-39	2.730310570097038E-5	0.0	0.0	3.3833189491471467	0.0
40-41	2.730310570097038E-5	0.0	0.0	4.561925764493785	0.0
42-43	2.730310570097038E-5	0.0	0.0	5.686718158503812	0.0
44-45	2.730310570097038E-5	0.0	0.0	7.0021681396237145	0.0
46-47	2.730310570097038E-5	0.0	0.0	8.511961627123124	0.0
48-49	2.730310570097038E-5	0.0	0.0	9.970821170937374	0.0
50-51	2.730310570097038E-5	0.0	0.0	11.359907628132792	0.0
52-53	2.730310570097038E-5	0.0	0.0	12.646907773112284	0.0
54-55	2.730310570097038E-5	0.0	0.0	13.993428688519892	0.0
56-57	2.730310570097038E-5	0.0	0.0	15.486881267257269	0.0
58-59	4.095465855145557E-5	0.0	0.0	17.02632592754793	0.0
60-61	5.460621140194076E-5	0.0	0.0	18.503696977027438	0.0
62-63	5.460621140194076E-5	0.0	0.0	19.861453120430994	0.0
64-65	8.190931710291114E-5	0.0	0.0	21.216574514134408	0.0
66-67	8.190931710291114E-5	0.0	0.0	22.678478354234315	0.0
68-69	8.190931710291114E-5	0.0	0.0	24.181350504438527	0.0
70-71	8.190931710291114E-5	0.0	0.0	25.67446179435465	0.0
72-73	8.190931710291114E-5	0.0	0.0	27.0827832895164	0.0
74-75	8.190931710291114E-5	0.0	0.0	28.436894468308875	0.0
76-77	8.190931710291114E-5	0.0	0.0	29.81955104411172	0.0
78-79	8.190931710291114E-5	0.0	0.0	31.24817239836214	0.0
80-81	8.190931710291114E-5	0.0	0.0	32.69004941043039	0.0
82-83	8.190931710291114E-5	0.0	0.0	34.06899276385789	0.0
84-85	8.190931710291114E-5	0.0	0.0	35.36063443680655	0.0
86-87	8.190931710291114E-5	0.0	0.0	36.660016540221434	0.0
88	8.190931710291114E-5	0.0	0.0	37.6551601368104	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3060	0.0	44.24974	1
GTATCAA	5145	0.0	35.72984	1
TCAACGC	6075	0.0	29.396734	4
GTACATG	8710	0.0	29.14844	1
ATCAACG	6145	0.0	29.138342	3
TACATGG	8525	0.0	28.170097	2
CAACGCA	6350	0.0	28.123648	5
TATCAAC	6745	0.0	26.685701	2
AACGCAG	6895	0.0	25.918367	6
GAGTACA	6440	0.0	25.770836	1
ACATGGG	9165	0.0	25.741451	3
AGTACAT	6655	0.0	22.527054	2
CATGGGG	5795	0.0	21.815271	4
ACGCAGA	8345	0.0	21.189459	7
CGCAGAG	8415	0.0	20.845535	8
ATGGGGG	3620	0.0	19.213873	5
TACACCG	495	0.0	18.988321	5
CCGTGCA	885	0.0	18.06733	9
GCAGAGT	9590	0.0	17.850119	9
AGTATCG	215	2.626755E-5	17.48692	5
>>END_MODULE