FastQCFastQC Report
Wed 25 May 2016
SRR2049373_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049373_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4933046
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48700.045.3708761
GTATCAA81300.036.8347051
TCAACGC89950.032.715614
ATCAACG90600.032.429013
CAACGCA96150.030.5085535
AACGCAG101600.028.774816
TATCAAC102650.028.7595792
GTACATG143450.025.1036281
ACGCAGA118600.024.0951847
TACATGG142650.023.5292632
CGCAGAG121200.023.2292738
ACATGGG150400.021.8792323
GCAGAGT140300.019.1290879
GTATAGG16000.018.5109581
CATGGGG100600.018.1774714
GAGTACA110450.018.0897061
GTGGTAT30150.017.1519571
AGAGTAC119850.017.11835510-11
TATACCG4154.7293724E-1116.9914115
CAGAGTA133850.016.2935410-11
Produced by FastQC (version 0.11.5)