##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049373_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4933046
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.656419583356815	31.0	31.0	34.0	28.0	34.0
2	30.896945213971247	31.0	31.0	34.0	27.0	34.0
3	31.066393461565127	31.0	31.0	34.0	28.0	34.0
4	34.68958387981787	37.0	35.0	37.0	32.0	37.0
5	34.72315482158488	37.0	35.0	37.0	32.0	37.0
6	34.718019049487886	37.0	35.0	37.0	32.0	37.0
7	34.627388635743515	37.0	35.0	37.0	32.0	37.0
8	34.59053067820572	37.0	35.0	37.0	32.0	37.0
9	36.189734699412895	39.0	37.0	39.0	32.0	39.0
10-11	36.15196929037353	39.0	37.0	39.0	32.0	39.0
12-13	36.08543514493884	39.0	37.0	39.0	31.0	39.0
14-15	37.28026314775901	40.0	38.0	41.0	31.0	41.0
16-17	37.24596354868777	40.0	37.0	41.0	31.0	41.0
18-19	37.281226953894205	40.0	37.5	41.0	31.0	41.0
20-21	37.35074576640883	40.0	38.0	41.0	31.0	41.0
22-23	37.30287250514185	40.0	38.0	41.0	31.0	41.0
24-25	37.306465923893676	40.0	37.5	41.0	31.0	41.0
26-27	37.29007939516477	40.0	37.0	41.0	31.0	41.0
28-29	37.2302479036279	40.0	37.0	41.0	31.0	41.0
30-31	37.167892413733824	40.0	37.0	41.0	31.0	41.0
32-33	37.10983548501271	40.0	37.0	41.0	31.0	41.0
34-35	37.01170473577582	40.0	37.0	41.0	30.0	41.0
36-37	36.93733567455077	40.0	37.0	41.0	30.0	41.0
38-39	36.836975167067166	40.0	37.0	41.0	30.0	41.0
40-41	36.735271777315674	40.0	37.0	41.0	30.0	41.0
42-43	36.603198713330464	40.0	36.5	41.0	30.0	41.0
44-45	36.486564183670694	40.0	36.0	41.0	30.0	41.0
46-47	36.3676730360917	40.0	36.0	41.0	30.0	41.0
48-49	36.239475873527226	40.0	36.0	41.0	29.0	41.0
50-51	36.10439229636212	39.5	35.5	41.0	29.0	41.0
52-53	35.96444640897328	39.0	35.0	41.0	28.5	41.0
54-55	35.83061479661856	39.0	35.0	41.0	28.0	41.0
56-57	35.70788048601209	39.0	35.0	41.0	28.0	41.0
58-59	35.508108884449896	39.0	35.0	41.0	28.0	41.0
60-61	35.296858472432646	39.0	35.0	40.0	28.0	41.0
62-63	35.09229226729286	38.0	34.0	40.0	27.5	41.0
64-65	34.85092608096498	38.0	34.0	40.0	27.0	41.0
66-67	34.59373285795429	37.5	34.0	40.0	26.5	41.0
68-69	34.312235786976245	37.0	34.0	40.0	26.0	41.0
70-71	34.029583648723325	36.5	34.0	39.5	26.0	41.0
72-73	33.687945743866976	36.0	33.5	39.0	26.0	41.0
74-75	33.34222962850944	35.5	33.0	39.0	26.0	40.5
76-77	33.00576905222453	35.0	33.0	38.0	25.0	40.0
78-79	32.68680831275443	35.0	33.0	37.0	25.0	39.0
80-81	32.3344262348253	35.0	33.0	37.0	24.5	39.0
82-83	32.02058190821654	35.0	33.0	36.0	24.0	39.0
84-85	31.728985093591263	35.0	32.0	36.0	23.0	37.0
86-87	31.411057488618592	35.0	32.0	36.0	22.0	37.0
88-89	31.164139661377575	35.0	32.0	35.0	20.5	37.0
90-91	30.961482824202328	35.0	32.0	35.0	20.0	36.0
92-93	30.729695506589643	35.0	32.0	35.0	18.5	36.0
94-95	30.53650746415095	35.0	32.0	35.0	16.5	36.0
96-97	30.289299451089654	35.0	32.0	35.0	4.5	36.0
98-99	30.094237515725577	35.0	32.0	35.0	2.0	35.0
100	29.978871269394205	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.2930824914580512
1101	2	0.25844132798110664
1101	3	0.3293548959973229
1101	4	0.4381433866792932
1101	5	0.4059502508449029
1101	6	0.5000327073503996
1101	7	0.4871932573734554
1101	8	0.4320195818859105
1101	9	0.5608765679193368
1101	10-11	0.5954515203084867
1101	12-13	0.6285133337551443
1101	14-15	0.7291844439913788
1101	16-17	0.690230871726726
1101	18-19	0.6586225988679573
1101	20-21	0.6710972050696355
1101	22-23	0.6760012860601634
1101	24-25	0.6918984751313317
1101	26-27	0.6078370182315851
1101	28-29	0.6290818571461827
1101	30-31	0.6475434814943668
1101	32-33	0.6067760665389699
1101	34-35	0.6475781140305088
1101	36-37	0.6442636426704027
1101	38-39	0.7262936512095308
1101	40-41	0.7753965619059286
1101	42-43	0.7492236600954598
1101	44-45	0.665966854295867
1101	46-47	0.6147232308691457
1101	48-49	0.5922066970966924
1101	50-51	0.7009510998572495
1101	52-53	0.703368467644971
1101	54-55	0.6591768676887568
1101	56-57	0.6369110160340696
1101	58-59	0.695155844777986
1101	60-61	0.71955572487051
1101	62-63	0.661039247532301
1101	64-65	0.7102516772434555
1101	66-67	0.6834237912434915
1101	68-69	0.7291040867200635
1101	70-71	0.752927304187395
1101	72-73	0.7251356935497029
1101	74-75	0.6981949263202836
1101	76-77	0.7330308629690521
1101	78-79	0.7074310801589547
1101	80-81	0.7171728442452476
1101	82-83	0.7511309735293139
1101	84-85	0.7097783615868707
1101	86-87	0.7431066405587217
1101	88-89	0.7308767317738472
1101	90-91	0.7346635998413653
1101	92-93	0.6780991906435148
1101	94-95	0.7395710404665081
1101	96-97	0.7289716567409741
1101	98-99	0.8064644752651304
1101	100	0.8661432894009025
1102	1	0.058328342730785465
1102	2	0.1402478276638668
1102	3	0.19789281627595656
1102	4	0.19220422997268827
1102	5	0.12610786495506687
1102	6	0.31867511065765086
1102	7	0.3253340305770891
1102	8	0.32480916687719485
1102	9	0.4315586241639693
1102	10-11	0.34304858024738394
1102	12-13	0.4076983164788288
1102	14-15	0.3908172995766037
1102	16-17	0.3516516799008613
1102	18-19	0.3524576761869511
1102	20-21	0.34357501630798026
1102	22-23	0.34757551275835397
1102	24-25	0.3741694030869738
1102	26-27	0.38619422425852434
1102	28-29	0.41284607293028586
1102	30-31	0.3138329002703202
1102	32-33	0.35818986749377046
1102	34-35	0.4171784005228574
1102	36-37	0.40580714921063077
1102	38-39	0.39811039148983696
1102	40-41	0.434356915159853
1102	42-43	0.37540827769505114
1102	44-45	0.3505827783525106
1102	46-47	0.24830315901189692
1102	48-49	0.30660395720916256
1102	50-51	0.3651513995454678
1102	52-53	0.4126328828895325
1102	54-55	0.33817438844534564
1102	56-57	0.3253919345357943
1102	58-59	0.42256295682464895
1102	60-61	0.3206994282005482
1102	62-63	0.35892114573699985
1102	64-65	0.3319539466585226
1102	66-67	0.40513948130317345
1102	68-69	0.39398702479994796
1102	70-71	0.3941020646218405
1102	72-73	0.3754747538352632
1102	74-75	0.3578136839760333
1102	76-77	0.41980283565280274
1102	78-79	0.4214003528901742
1102	80-81	0.3400779961483309
1102	82-83	0.3480745953996305
1102	84-85	0.317871304536645
1102	86-87	0.37318850669526427
1102	88-89	0.3990216343594355
1102	90-91	0.4108943692969014
1102	92-93	0.42235015163052836
1102	94-95	0.3843059088684839
1102	96-97	0.3481006687561461
1102	98-99	0.45599140060709686
1102	100	0.4181529563517117
1103	1	0.10476356581960644
1103	2	0.1552296236600128
1103	3	0.1709612869959969
1103	4	0.221998880054322
1103	5	0.20668317029875993
1103	6	0.28701758532149313
1103	7	0.28076277710334807
1103	8	0.3151548548572194
1103	9	0.4017140438820235
1103	10-11	0.40443441005653114
1103	12-13	0.3454382293814007
1103	14-15	0.42446854465112693
1103	16-17	0.424539033949074
1103	18-19	0.44273934407451776
1103	20-21	0.42127345294553464
1103	22-23	0.4034374892692725
1103	24-25	0.4307970899986273
1103	26-27	0.3625187596169752
1103	28-29	0.3897069133581539
1103	30-31	0.32408281802228345
1103	32-33	0.38408360098004124
1103	34-35	0.3927158170522205
1103	36-37	0.3563172922230393
1103	38-39	0.4157317167467909
1103	40-41	0.43080161130722416
1103	42-43	0.3896822960023414
1103	44-45	0.323974701014059
1103	46-47	0.34875813941258116
1103	48-49	0.42358084089435977
1103	50-51	0.32390255631877096
1103	52-53	0.375085372252002
1103	54-55	0.34729219020329793
1103	56-57	0.3128471427002708
1103	58-59	0.38611384219653644
1103	60-61	0.3139104941008881
1103	62-63	0.29983511463390045
1103	64-65	0.2993829038491924
1103	66-67	0.3761249051045681
1103	68-69	0.3441649609864186
1103	70-71	0.2692958519369668
1103	72-73	0.32840398085420475
1103	74-75	0.30928789023974446
1103	76-77	0.35219782320787374
1103	78-79	0.2322847945713562
1103	80-81	0.21000989272305048
1103	82-83	0.1824425782400496
1103	84-85	0.3015240885556949
1103	86-87	0.25937391462842285
1103	88-89	0.24233492037535598
1103	90-91	0.29280958234496524
1103	92-93	0.24882184428484067
1103	94-95	0.26702889680110786
1103	96-97	0.2392731461353712
1103	98-99	0.28561977628192636
1103	100	0.2146211812510117
1104	1	-0.002949040017281135
1104	2	0.06947043331349079
1104	3	0.13004067051900847
1104	4	0.17789908713130131
1104	5	0.1877889346345114
1104	6	0.20121883523609796
1104	7	0.2318045890372531
1104	8	0.2418340217991286
1104	9	0.30679221953441527
1104	10-11	0.3131394346138734
1104	12-13	0.2685397806614205
1104	14-15	0.3901132074833953
1104	16-17	0.4512698713301333
1104	18-19	0.4096158730857127
1104	20-21	0.4078048580521241
1104	22-23	0.38564045293735916
1104	24-25	0.47661077755535786
1104	26-27	0.33388495199963586
1104	28-29	0.41882956228730706
1104	30-31	0.32404698465272475
1104	32-33	0.31893803638521945
1104	34-35	0.39069763048796347
1104	36-37	0.3352984708585893
1104	38-39	0.40939542094985626
1104	40-41	0.43684759846856736
1104	42-43	0.36506471465315116
1104	44-45	0.3288095334582337
1104	46-47	0.27055935718254887
1104	48-49	0.24564058965049895
1104	50-51	0.32935674710098084
1104	52-53	0.31906402053223104
1104	54-55	0.2911293493698892
1104	56-57	0.3179320371094505
1104	58-59	0.38405009229840203
1104	60-61	0.33389039240864093
1104	62-63	0.32160569336729594
1104	64-65	0.35233034905603233
1104	66-67	0.3626626431569875
1104	68-69	0.3418687176371904
1104	70-71	0.38258870523112165
1104	72-73	0.3884464614643832
1104	74-75	0.42801224674940386
1104	76-77	0.425999442597643
1104	78-79	0.3579010993567522
1104	80-81	0.39293165985771594
1104	82-83	0.31784202338742773
1104	84-85	0.3539687656159174
1104	86-87	0.43089804484583283
1104	88-89	0.33928412488020143
1104	90-91	0.36099125338823157
1104	92-93	0.399278239835553
1104	94-95	0.4196058223318744
1104	96-97	0.38824193929797346
1104	98-99	0.3974830117528967
1104	100	0.461167824949694
1105	1	-5.56248683466265E-4
1105	2	0.04863403404938893
1105	3	0.08326174557845434
1105	4	0.09197108131461817
1105	5	0.0716961021950766
1105	6	0.17804717066233167
1105	7	0.11602396270991733
1105	8	0.1915562402793256
1105	9	0.256075092107352
1105	10-11	0.25985129440474
1105	12-13	0.20791638060396878
1105	14-15	0.20805309861357557
1105	16-17	0.21031298053163283
1105	18-19	0.2114493148568144
1105	20-21	0.26847455199547454
1105	22-23	0.25262029661949015
1105	24-25	0.23475953912564052
1105	26-27	0.21544988195078218
1105	28-29	0.2557379499093102
1105	30-31	0.19519728304166506
1105	32-33	0.23201191764057683
1105	34-35	0.26732415151427347
1105	36-37	0.16791665498045916
1105	38-39	0.1234270012807599
1105	40-41	0.1579563361255012
1105	42-43	0.19226835277368792
1105	44-45	0.1962446519308969
1105	46-47	0.15106620878687949
1105	48-49	0.13949062076188312
1105	50-51	0.11270598504499674
1105	52-53	0.14878911895453228
1105	54-55	0.18397169201892183
1105	56-57	0.1777894490281895
1105	58-59	0.22433452936074616
1105	60-61	0.20702562990192064
1105	62-63	0.1556564029216716
1105	64-65	0.14440331754087765
1105	66-67	0.1805459366893487
1105	68-69	0.1542976338578157
1105	70-71	0.2202626804942085
1105	72-73	0.22779737494487762
1105	74-75	0.2787737748008041
1105	76-77	0.33085245450371303
1105	78-79	0.2169319569714645
1105	80-81	0.25435113998994296
1105	82-83	0.1918548058156304
1105	84-85	0.19212379315377603
1105	86-87	0.15125777910473204
1105	88-89	0.18647203072539043
1105	90-91	0.23103413940333084
1105	92-93	0.21143576429412647
1105	94-95	0.14790871723801757
1105	96-97	0.18050654590188842
1105	98-99	0.20701642108368645
1105	100	0.1930484562650392
1106	1	0.07756255982598148
1106	2	0.10489906935698556
1106	3	0.19223285246524924
1106	4	0.21278208635207108
1106	5	0.29359004231667285
1106	6	0.3002152936709024
1106	7	0.27634079238733733
1106	8	0.27724214719162177
1106	9	0.3615707999227027
1106	10-11	0.37798334053383087
1106	12-13	0.3714480482908158
1106	14-15	0.4847410042667164
1106	16-17	0.4974685164500343
1106	18-19	0.4771342787196886
1106	20-21	0.4107621104667203
1106	22-23	0.4102402232537443
1106	24-25	0.4731932126262137
1106	26-27	0.36560421897393525
1106	28-29	0.4421869846370967
1106	30-31	0.47334080293869363
1106	32-33	0.42754443931106323
1106	34-35	0.4921080141923042
1106	36-37	0.4239067215265706
1106	38-39	0.41776189384734863
1106	40-41	0.4357244263019808
1106	42-43	0.3860599877506914
1106	44-45	0.34411632910062906
1106	46-47	0.3971330814691001
1106	48-49	0.4498769130146485
1106	50-51	0.471742319516693
1106	52-53	0.47277169524329565
1106	54-55	0.46528542971670817
1106	56-57	0.3883985367064611
1106	58-59	0.4410646190115557
1106	60-61	0.4827085330271714
1106	62-63	0.4339687112010324
1106	64-65	0.38849471998634044
1106	66-67	0.45898306803780287
1106	68-69	0.46801140613408876
1106	70-71	0.43494853588805427
1106	72-73	0.4707326249970478
1106	74-75	0.5141074789504785
1106	76-77	0.5356333221266425
1106	78-79	0.41223054999412057
1106	80-81	0.45150767134938974
1106	82-83	0.385542661696725
1106	84-85	0.4070975405789454
1106	86-87	0.44350202715816067
1106	88-89	0.4216783795436534
1106	90-91	0.5010947215966333
1106	92-93	0.5060508211259531
1106	94-95	0.4555719979497752
1106	96-97	0.3950213426092475
1106	98-99	0.419632588618164
1106	100	0.3726186003682308
1107	1	0.08700107942982527
1107	2	0.0941631124305573
1107	3	0.10572118570947708
1107	4	0.1253022992944679
1107	5	0.15447582311486485
1107	6	0.25235685353016635
1107	7	0.2353472285878837
1107	8	0.24438865790868647
1107	9	0.29999666456350127
1107	10-11	0.2638299081810658
1107	12-13	0.24782870733821483
1107	14-15	0.23961181309699242
1107	16-17	0.3256223631819921
1107	18-19	0.3056599991818061
1107	20-21	0.25261001109632986
1107	22-23	0.26248451533402317
1107	24-25	0.3244714767975623
1107	26-27	0.3016348744941624
1107	28-29	0.3619256029164788
1107	30-31	0.2965716932851805
1107	32-33	0.3295747863527936
1107	34-35	0.2858216972974361
1107	36-37	0.3004518084941026
1107	38-39	0.34285973670129977
1107	40-41	0.4336816786297035
1107	42-43	0.35553613455707733
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2214	1	0.06964972061946995
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2214	96-97	0.017602004438415975
2214	98-99	0.12749208203198492
2214	100	0.09347481678148029
2215	1	-0.1835613406885379
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2215	24-25	-0.5556957398383986
2215	26-27	-0.5145832142471178
2215	28-29	-0.4679616707793244
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2215	92-93	-0.09359108078978551
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2215	96-97	0.01899519328847532
2215	98-99	-0.17253058817115274
2215	100	-0.09826907750733227
2216	1	-1.2245639743563572
2216	2	-1.2005833079671255
2216	3	-1.3128082856041168
2216	4	-1.5891372792785532
2216	5	-1.4986677446822014
2216	6	-2.8681487657773523
2216	7	-2.819425055559286
2216	8	-3.681926857446843
2216	9	-3.1359010024845304
2216	10-11	-2.3126332974495085
2216	12-13	-2.108921822524678
2216	14-15	-2.2912996382408295
2216	16-17	-2.733416378227581
2216	18-19	-2.393804354300812
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2216	24-25	-2.4913021117312084
2216	26-27	-2.5477802687556235
2216	28-29	-3.025995512773001
2216	30-31	-2.537901877787654
2216	32-33	-2.653263082235192
2216	34-35	-3.6973096877216562
2216	36-37	-2.6728333360829737
2216	38-39	-2.619511877002701
2216	40-41	-3.1019849615201665
2216	42-43	-2.930933840537726
2216	44-45	-2.6186830918042574
2216	46-47	-2.5422834465185247
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2216	50-51	-3.0690447576509428
2216	52-53	-3.383677341709152
2216	54-55	-2.79191983566092
2216	56-57	-2.7539369846318706
2216	58-59	-3.8313555483047423
2216	60-61	-4.488487233048495
2216	62-63	-3.6314455736842675
2216	64-65	-3.516312959863292
2216	66-67	-3.2114184642383847
2216	68-69	-3.6253314421535663
2216	70-71	-3.415776302767746
2216	72-73	-3.8189050919133223
2216	74-75	-4.290430323479839
2216	76-77	-5.045140693441901
2216	78-79	-3.9534293171394026
2216	80-81	-3.786147040559271
2216	82-83	-4.035993923854907
2216	84-85	-4.05468007756928
2216	86-87	-3.657922035787532
2216	88-89	-3.6094013240866687
2216	90-91	-3.8392535583971217
2216	92-93	-3.8914990729026577
2216	94-95	-3.756480943222538
2216	96-97	-4.045208382603256
2216	98-99	-3.796649314204135
2216	100	-3.4774771943763625
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	76065.0
3	13880.0
4	8812.0
5	7116.0
6	6436.0
7	6809.0
8	7255.0
9	8254.0
10	9719.0
11	10885.0
12	12271.0
13	13732.0
14	15319.0
15	15888.0
16	16673.0
17	17769.0
18	16881.0
19	15372.0
20	15265.0
21	16161.0
22	18034.0
23	21213.0
24	26251.0
25	32015.0
26	39269.0
27	46148.0
28	56408.0
29	70242.0
30	85893.0
31	101738.0
32	129649.0
33	170837.0
34	234386.0
35	337018.0
36	546717.0
37	959188.0
38	1280092.0
39	466225.0
40	1161.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.316177342706	20.434133724557313	14.336039420844152	22.91364951189253
2	14.976396178369008	20.177226890125777	41.171416328665295	23.67496060283992
3	18.067580927273188	25.677690182260644	30.43321757205506	25.821511318411112
4	12.373395330236328	16.880230557742966	39.461739903009516	31.284634209011198
5	12.796583478389076	38.268858818425905	35.895907065355495	13.038650637829525
6	30.540019833986694	38.30311928987027	17.845680226650956	13.311180649492083
7	26.664619761302983	32.89030811266064	22.858673690499394	17.58639843553698
8	24.506021755667216	36.900392351547964	20.74579850039657	17.847787392388252
9	24.655752133424187	17.14425354489114	21.969787306531686	36.230207015152985
10-11	23.525061153700918	27.33835082329856	29.291720289806282	19.844867733194242
12-13	23.86680073080911	24.903289878082884	29.40854675132068	21.82136263978732
14-15	21.29119022469806	27.148342656756245	26.55864955046497	25.001817568080725
16-17	20.793347348833663	30.564109354971308	27.17466535069406	21.46787794550097
18-19	20.723999977692504	29.546625458634544	29.200514492015184	20.528860071657768
20-21	21.88039084565774	28.643878321328746	28.797059075531283	20.678671757482224
22-23	21.51860870284704	28.47618956843498	28.858187084989755	21.147014643728227
24-25	21.323646016478783	28.676495937086184	28.741977088694615	21.257880957740426
26-27	21.10465752357923	28.630087219250267	28.8479148100759	21.417340447094602
28-29	21.093541108503093	28.691474131814935	28.971276251537798	21.243708508144174
30-31	21.424869749193874	28.985201147924744	28.527205416900426	21.062723685980956
32-33	21.12371011718326	28.758143359632054	28.916260153533695	21.201886369650985
34-35	21.33184362889244	28.53428880453449	28.89316846000029	21.240699106572777
36-37	21.509795993025776	28.816912855874577	28.479760517607723	21.193530633491925
38-39	21.334570199363025	28.966259372185377	28.569658529868665	21.129511898582933
40-41	21.296106462838935	28.690276225593532	28.726981116043625	21.286636195523908
42-43	21.19737720502885	28.711245961452196	28.688959423494513	21.40241741002444
44-45	21.295008977359693	28.74577139387755	28.614680041384677	21.34453958737808
46-47	21.325244657206152	28.851355468702867	28.48894879148937	21.33445108260161
48-49	21.281674856299098	28.73892300799185	28.495297370195686	21.484104765513365
50-51	21.30202815284246	28.733850097758978	28.44377213493386	21.5203496144647
52-53	21.293702014590007	28.690657939470338	28.532057227257624	21.48358281868203
54-55	21.42454103443133	28.932795230933472	28.35577460170327	21.28688913293193
56-57	21.31188313815566	28.77797541307811	28.371911083784873	21.538230364981352
58-59	21.269761668613743	28.432934988898527	28.674827282798322	21.62247605968941
60-61	21.436773340479462	28.745007823935943	28.36109606280563	21.45712277277897
62-63	21.290350185588952	28.810038594066345	28.395261884831026	21.504349335513677
64-65	21.356643375077855	28.577519197893608	28.468575923323403	21.597261503705138
66-67	21.226321403835783	28.64072024522697	28.418452126933126	21.714506224004126
68-69	21.552860072782902	28.712410608150503	28.33860028576191	21.396129033304685
70-71	21.500314671988292	28.607308521816293	28.38871617783089	21.503660628364525
72-73	21.332135312298615	28.695067551920665	28.47081374990723	21.50198338587349
74-75	21.461258928554226	28.676971500917738	28.369252020632107	21.49251754989593
76-77	21.47497450267931	28.68068614585248	28.254782794461736	21.589556557006475
78-79	21.477520986229333	28.763704177839568	28.356344262878498	21.402430573052598
80-81	21.443017539811105	28.635140071803413	28.392500154368506	21.52934223401698
82-83	21.38087315178411	28.69712629868122	28.47596394160332	21.446036607931347
84-85	21.401441316447105	28.549318047866585	28.3737389089435	21.67550172674281
86-87	21.542339379148505	28.67371102061343	28.300021951292464	21.483927648945603
88-89	21.371655655138444	28.60413189337518	28.35954354864908	21.6646689028373
90-91	21.369572614631256	28.55029693790767	28.406177719862413	21.673952727598657
92-93	21.430756407132783	28.71014175796176	28.279797302935467	21.579304531969985
94-95	21.36396206345152	28.48198750055639	28.42300758242258	21.7310428535695
96-97	21.453134635274026	28.476472751318354	28.40683018159571	21.663562431811904
98-99	21.453732643076915	28.551416305463196	28.325430170324783	21.669420881135103
100	21.444235468309035	28.493328462779388	28.306810842631506	21.75562522628007
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1701.0
1	1362.5
2	1198.0
3	1381.5
4	1293.5
5	1074.0
6	852.5
7	759.0
8	780.0
9	787.0
10	741.0
11	674.5
12	695.0
13	798.5
14	918.5
15	1066.5
16	1284.0
17	1607.5
18	2192.5
19	3120.0
20	4554.0
21	6666.0
22	9594.5
23	13974.5
24	20055.0
25	28198.5
26	38467.5
27	50795.5
28	65958.5
29	82550.5
30	99060.0
31	112870.0
32	124217.0
33	136083.0
34	147234.0
35	156000.0
36	162479.5
37	169593.5
38	176577.5
39	180275.0
40	183171.0
41	184590.5
42	183159.5
43	180524.0
44	176708.5
45	172476.5
46	168168.5
47	161569.5
48	154169.5
49	149063.5
50	144335.0
51	137039.0
52	127344.5
53	118926.5
54	112455.0
55	106471.5
56	97564.5
57	87308.5
58	79160.0
59	71348.5
60	62811.5
61	53515.0
62	45098.0
63	37522.5
64	30351.5
65	24086.5
66	18968.5
67	14581.5
68	10966.5
69	8404.0
70	6270.0
71	4730.0
72	3500.0
73	2366.5
74	1664.5
75	1187.5
76	794.5
77	538.0
78	376.5
79	261.5
80	181.0
81	130.0
82	100.5
83	76.0
84	59.5
85	44.5
86	33.5
87	27.5
88	22.5
89	20.5
90	13.0
91	13.0
92	14.5
93	10.5
94	12.0
95	11.0
96	8.0
97	9.5
98	9.0
99	12.5
100	29.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016886118637450372
2	0.037562998601675314
3	0.03894145726595698
4	0.04149566008506712
5	0.044252577413630444
6	0.0428943902002941
7	0.041049688164270105
8	0.040725344949145016
9	0.040664530596309055
10-11	0.041110502517106066
12-13	0.0420227178096454
14-15	0.04067466632178171
16-17	0.037279198288440854
18-19	0.03990435118585961
20-21	0.039397564912226644
22-23	0.04040100173401991
24-25	0.03762381295451127
26-27	0.03755286287620265
28-29	0.03816100640456221
30-31	0.03824209220834349
32-33	0.038130599228144234
34-35	0.03783666318943711
36-37	0.03535341044863559
38-39	0.03578924664395994
40-41	0.03695485507331576
42-43	0.03685349781858916
44-45	0.03551558205619814
46-47	0.03461350248913146
48-49	0.0346540453910221
50-51	0.036194675662866306
52-53	0.036377118721374176
54-55	0.035809518094905256
56-57	0.03414725911738914
58-59	0.03484662417500263
60-61	0.03455268813629551
62-63	0.034826352724057305
64-65	0.032039028219076
66-67	0.03272825755121683
68-69	0.03381278017679138
70-71	0.034877031351420604
72-73	0.03202889249360334
74-75	0.03256608594365428
76-77	0.03335667253052171
78-79	0.03359992994186553
80-81	0.033741830098482764
82-83	0.032454592963455035
84-85	0.03347830123619362
86-87	0.03392427315699063
88-89	0.034441195156096255
90-91	0.0347047240183854
92-93	0.03339721543241234
94-95	0.034593231038186144
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4933046.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.86975432839202
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.67800140378208	50.389528677838335
2	13.625904581045964	17.67818165349126
3	4.1660935723278865	8.107603996380092
4	1.7085189489340655	4.433248179310215
5	0.8538622962111162	2.76949186927459
6	0.4965905452262791	1.9328224002378582
7	0.29454256960172426	1.3374832890522013
8	0.21032651407724207	1.0915063437550223
9	0.15676796537538357	0.9152549460416699
>10	0.7627312860711178	8.547503145028509
>50	0.035166707152929744	1.5426321373677592
>100	0.011368467435002364	1.1653035364718063
>500	6.25714103545174E-5	0.030401844861352834
>1k	6.257134887563717E-5	0.05903798088922193
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.027145094531857E-5	0.0
5	0.0	0.0	0.0	2.027145094531857E-5	0.0
6	0.0	0.0	0.0	2.027145094531857E-5	0.0
7	0.0	0.0	0.0	8.108580378127429E-5	0.0
8	0.0	0.0	0.0	8.108580378127429E-5	0.0
9	6.0814352835955715E-5	0.0	0.0	1.4190015661723002E-4	0.0
10-11	6.0814352835955715E-5	0.0	0.0	1.520358820898893E-4	0.0
12-13	6.0814352835955715E-5	0.0	0.0	1.8244305850786716E-4	0.0
14-15	8.108580378127429E-5	0.0	0.0	2.0271450945318572E-4	0.0
16-17	8.108580378127429E-5	0.0	0.0	2.0271450945318572E-4	0.0
18-19	8.108580378127429E-5	0.0	0.0	2.0271450945318572E-4	0.0
20-21	8.108580378127429E-5	0.0	0.0	2.3312168587116355E-4	0.0
22-23	8.108580378127429E-5	0.0	0.0	3.54750391543075E-4	0.0
24-25	8.108580378127429E-5	0.0	0.0	5.574649009962607E-4	0.0
26-27	8.108580378127429E-5	0.0	0.0	9.730296453752914E-4	0.0
28-29	8.108580378127429E-5	0.0	0.0	0.0027873245049813036	0.0
30-31	8.108580378127429E-5	0.0	0.0	0.007125415007279478	0.0
32-33	8.108580378127429E-5	0.0	0.0	0.017889555459243638	0.0
34-35	1.0135725472659286E-4	0.0	0.0	0.03391413743151797	0.0
36-37	1.0135725472659286E-4	0.0	0.0	0.06094611726710029	0.0
38-39	1.0135725472659286E-4	0.0	0.0	0.09640088497046247	0.0
40-41	1.0135725472659286E-4	0.0	0.0	0.13281854659372727	0.0
42-43	1.0135725472659286E-4	0.0	0.0	0.1672394702988782	0.0
44-45	1.1149298019925215E-4	0.0	0.0	0.21150218343798133	0.0
46-47	1.2162870567191143E-4	0.0	0.0	0.26332614777968827	0.0
48-49	1.2162870567191143E-4	0.0	0.0	0.31653870651114946	0.0
50-51	1.2162870567191143E-4	0.0	0.0	0.3700654727322632	0.0
52-53	1.2162870567191143E-4	0.0	0.0	0.42297395969954465	0.0
54-55	1.2162870567191143E-4	0.0	0.0	0.48089963077579245	0.0
56-57	1.317644311445707E-4	0.0	0.0	0.5496603923823131	0.0
58-59	1.4190015661723002E-4	0.0	0.0	0.6245431321743199	0.0
60-61	1.4190015661723002E-4	0.0	0.0	0.7009259593362802	0.0
62-63	1.4190015661723002E-4	0.0	0.0	0.7748052623064938	0.0
64-65	1.4190015661723002E-4	0.0	0.0	0.854644371854631	0.0
66-67	1.4190015661723002E-4	0.0	0.0	0.9457442724028926	0.0
68-69	1.4190015661723002E-4	0.0	0.0	1.0443952884282854	0.0
70-71	1.4190015661723002E-4	0.0	0.0	1.148022945660754	0.0
72-73	1.4190015661723002E-4	0.0	0.0	1.256606972649353	0.0
74-75	1.6217160756254857E-4	0.0	0.0	1.3644004130510845	0.0
76-77	1.6217160756254857E-4	0.0	0.0	1.4804139268111425	0.0
78-79	1.6217160756254857E-4	0.0	0.0	1.6101816200375993	0.0
80-81	1.6217160756254857E-4	0.0	0.0	1.750875625323583	0.0
82-83	1.6217160756254857E-4	0.0	0.0	1.8936271828805165	0.0
84-85	1.6217160756254857E-4	0.0	0.0	2.037301091455462	0.0
86-87	1.6217160756254857E-4	0.0	0.0	2.189722131113312	0.0
88	1.6217160756254857E-4	0.0	0.0	2.315364584072397	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4870	0.0	45.370876	1
GTATCAA	8130	0.0	36.834705	1
TCAACGC	8995	0.0	32.71561	4
ATCAACG	9060	0.0	32.42901	3
CAACGCA	9615	0.0	30.508553	5
AACGCAG	10160	0.0	28.77481	6
TATCAAC	10265	0.0	28.759579	2
GTACATG	14345	0.0	25.103628	1
ACGCAGA	11860	0.0	24.095184	7
TACATGG	14265	0.0	23.529263	2
CGCAGAG	12120	0.0	23.229273	8
ACATGGG	15040	0.0	21.879232	3
GCAGAGT	14030	0.0	19.129087	9
GTATAGG	1600	0.0	18.510958	1
CATGGGG	10060	0.0	18.177471	4
GAGTACA	11045	0.0	18.089706	1
GTGGTAT	3015	0.0	17.151957	1
AGAGTAC	11985	0.0	17.118355	10-11
TATACCG	415	4.7293724E-11	16.991411	5
CAGAGTA	13385	0.0	16.29354	10-11
>>END_MODULE