FastQCFastQC Report
Wed 25 May 2016
SRR2049373_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049373_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4933046
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119750.2427506250701899No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113380.22983771081802198No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67810.13746070886020523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81000.056.0686651
GTATCAA144200.040.004371
ATCAACG175150.032.3588873
TCAACGC175650.032.3202864
CAACGCA181150.031.2871045
TATCAAC183700.031.1342092
AACGCAG188350.030.147226
GTGGTAT36400.027.1235181
ACGCAGA217900.025.8213887
TGGTATC35900.025.5268122
CGCAGAG221250.025.2390548
GTACATG136400.023.3002431
GCAGAGT245050.022.3270139
TACATGG137350.022.3087542
GAGTACT168850.021.49795712-13
ACATGGG142550.020.7037413
CAGAGTA238100.020.24222410-11
AGAGTAC224250.019.97147610-11
GTACTTT184900.019.44398914-15
AGTACTT177800.019.0009812-13
Produced by FastQC (version 0.11.5)