##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049373_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4933046
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.792865908811716	31.0	31.0	34.0	30.0	34.0
2	31.919893307299386	33.0	31.0	34.0	30.0	34.0
3	31.985520913447797	33.0	31.0	34.0	30.0	34.0
4	35.732357654885035	37.0	35.0	37.0	33.0	37.0
5	35.765603645293396	37.0	35.0	37.0	35.0	37.0
6	35.854364423117076	37.0	35.0	37.0	35.0	37.0
7	35.807572035614506	37.0	35.0	37.0	35.0	37.0
8	35.82013547005238	37.0	35.0	37.0	35.0	37.0
9	37.55212681981883	39.0	37.0	39.0	35.0	39.0
10-11	37.50977975879406	39.0	37.0	39.0	35.0	39.0
12-13	37.454969708370854	39.0	37.0	39.0	34.5	39.0
14-15	38.75676032617575	40.0	38.0	41.0	35.0	41.0
16-17	38.78523380888806	40.0	38.0	41.0	35.0	41.0
18-19	38.85401240937141	40.0	38.0	41.0	35.0	41.0
20-21	38.887148224443884	40.0	38.0	41.0	35.0	41.0
22-23	38.856623473610426	40.0	38.0	41.0	35.0	41.0
24-25	38.84021322728391	40.0	38.0	41.0	35.0	41.0
26-27	38.733655635889065	40.0	38.0	41.0	34.5	41.0
28-29	38.65819515974512	40.0	38.0	41.0	34.0	41.0
30-31	38.56300589939765	40.0	38.0	41.0	34.0	41.0
32-33	38.43404450313255	40.0	38.0	41.0	34.0	41.0
34-35	38.3375529034191	40.0	38.0	41.0	34.0	41.0
36-37	38.23138290216633	40.0	38.0	41.0	34.0	41.0
38-39	38.14815795352405	40.0	38.0	41.0	34.0	41.0
40-41	38.04420068249921	40.0	38.0	41.0	33.0	41.0
42-43	37.914203415090796	40.0	38.0	41.0	33.0	41.0
44-45	37.74852656958804	40.0	37.0	41.0	33.0	41.0
46-47	37.529405259955006	40.0	37.0	41.0	33.0	41.0
48-49	37.3320459610553	40.0	37.0	41.0	32.0	41.0
50-51	37.193750676559674	40.0	37.0	41.0	32.0	41.0
52-53	37.06568781235772	40.0	37.0	41.0	31.5	41.0
54-55	36.93155131738078	40.0	36.0	41.0	31.0	41.0
56-57	36.794485699099496	39.5	36.0	41.0	31.0	41.0
58-59	36.61452254854303	39.0	35.5	41.0	31.0	41.0
60-61	36.442193119626296	39.0	35.0	41.0	31.0	41.0
62-63	36.24857907264599	39.0	35.0	41.0	31.0	41.0
64-65	36.026744733375686	38.5	35.0	40.5	30.0	41.0
66-67	35.767033998872094	38.0	35.0	40.0	30.0	41.0
68-69	35.48729902376746	37.0	35.0	40.0	30.0	41.0
70-71	35.16010179106378	37.0	34.5	40.0	29.0	41.0
72-73	34.86405772417285	36.5	34.0	39.0	29.0	41.0
74-75	34.55927058048921	36.0	34.0	39.0	29.0	41.0
76-77	34.23069063211655	35.5	34.0	38.5	29.0	40.0
78-79	33.891600443215	35.0	34.0	37.5	29.0	39.5
80-81	33.56721952319115	35.0	34.0	37.0	29.0	39.0
82-83	33.2606019688444	35.0	34.0	36.5	29.0	39.0
84-85	32.98167744634856	35.0	34.0	36.0	28.0	37.5
86-87	32.74419141844613	35.0	33.5	36.0	28.0	37.0
88-89	32.514448780733034	35.0	33.0	35.5	27.5	37.0
90-91	32.31814298913896	35.0	33.0	35.0	27.0	36.0
92-93	32.133419595114255	35.0	33.0	35.0	27.0	36.0
94-95	31.976886390274892	35.0	33.0	35.0	27.0	36.0
96-97	31.779968907648538	35.0	33.0	35.0	26.0	36.0
98-99	31.63406199739471	35.0	33.0	35.0	26.0	35.5
100	31.52190188374485	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.08576180866086247
1101	2	0.13047601255486185
1101	3	0.33544413225773795
1101	4	0.17723800971104708
1101	5	0.146697435220986
1101	6	0.1553357990476485
1101	7	0.06620135804720917
1101	8	0.11455570069163201
1101	9	0.219451940545774
1101	10-11	0.24146886271248746
1101	12-13	0.20734853155703092
1101	14-15	0.2958691316960582
1101	16-17	0.1903228665989687
1101	18-19	0.255672732708085
1101	20-21	0.22443627705698788
1101	22-23	0.2708431400175826
1101	24-25	0.22387070218641725
1101	26-27	0.3029181164029069
1101	28-29	0.2912467062423403
1101	30-31	0.32886291649705157
1101	32-33	0.3197523996846101
1101	34-35	0.3119514045583216
1101	36-37	0.3340725257735997
1101	38-39	0.2829624271246871
1101	40-41	0.33493351917999803
1101	42-43	0.37836085830533506
1101	44-45	0.3631196523243432
1101	46-47	0.3797587533855662
1101	48-49	0.41906346776955417
1101	50-51	0.3280242016317203
1101	52-53	0.36309593212074986
1101	54-55	0.3419027944525368
1101	56-57	0.29023891575741345
1101	58-59	0.30236623260604745
1101	60-61	0.32048466822883626
1101	62-63	0.3291694868384596
1101	64-65	0.2633877873210224
1101	66-67	0.3059752815228336
1101	68-69	0.3597735077061728
1101	70-71	0.39801761269415437
1101	72-73	0.24114361338720158
1101	74-75	0.3594063243992096
1101	76-77	0.3114106756477213
1101	78-79	0.326274245819441
1101	80-81	0.39516752811280753
1101	82-83	0.3108163277168501
1101	84-85	0.35525861606631537
1101	86-87	0.2813007680578963
1101	88-89	0.3126009943269423
1101	90-91	0.36539011490312134
1101	92-93	0.34981778439282607
1101	94-95	0.39023717169244776
1101	96-97	0.38111143334179687
1101	98-99	0.38861727537906887
1101	100	0.4457186957207071
1102	1	0.04423874694430907
1102	2	0.035805440851131465
1102	3	0.26945608133113197
1102	4	0.0856864505797077
1102	5	0.0849303078026935
1102	6	0.10311202091428129
1102	7	0.1324064523465438
1102	8	0.12422724900880411
1102	9	0.06474958716994905
1102	10-11	0.10049011970175314
1102	12-13	0.13345170987339827
1102	14-15	0.16439305510872515
1102	16-17	0.15287401671530887
1102	18-19	0.1644874419307527
1102	20-21	0.1359903466335055
1102	22-23	0.17522925106438692
1102	24-25	0.15146087893659654
1102	26-27	0.19552818392563154
1102	28-29	0.17858532927397874
1102	30-31	0.09908737126139755
1102	32-33	0.15621542884302642
1102	34-35	0.149749121728739
1102	36-37	0.146574401813389
1102	38-39	0.14004166493587178
1102	40-41	0.1018867326836741
1102	42-43	0.11233265374177392
1102	44-45	0.1192170702662878
1102	46-47	0.05253738485865256
1102	48-49	0.007156268502988894
1102	50-51	0.06390222717945448
1102	52-53	0.11796658095232004
1102	54-55	0.059195812016852756
1102	56-57	0.09440240920514498
1102	58-59	0.07888325942569452
1102	60-61	0.0781354828760712
1102	62-63	0.11757386802506886
1102	64-65	0.07183572144184325
1102	66-67	0.0856611885811489
1102	68-69	0.13366005344250453
1102	70-71	0.09015839492933964
1102	72-73	0.15733181030213927
1102	74-75	0.09687522147293492
1102	76-77	0.0953896973493471
1102	78-79	0.16543857815963747
1102	80-81	0.1504843806847589
1102	82-83	0.1579412703016061
1102	84-85	0.1359305870071097
1102	86-87	0.08852875781759195
1102	88-89	0.0521419894820454
1102	90-91	0.13340949114270018
1102	92-93	0.1584953079822924
1102	94-95	0.16692012778231913
1102	96-97	0.17795490303858585
1102	98-99	0.14466498912178238
1102	100	0.14969041575796993
1103	1	0.11268757977457966
1103	2	0.11031593679481588
1103	3	0.28562566891451624
1103	4	0.17583234936093817
1103	5	0.16404491539577748
1103	6	0.09013523024972159
1103	7	0.10137093419076137
1103	8	0.11555485199667714
1103	9	0.14908388422454522
1103	10-11	0.13885915312830832
1103	12-13	0.19438755230189741
1103	14-15	0.27726082010300246
1103	16-17	0.2134470102223318
1103	18-19	0.21421935779357426
1103	20-21	0.22415900948222145
1103	22-23	0.22598111096434792
1103	24-25	0.2675672671979399
1103	26-27	0.21428627620895213
1103	28-29	0.2666246523846283
1103	30-31	0.2288712829300792
1103	32-33	0.27631794306800117
1103	34-35	0.24671179240623786
1103	36-37	0.2594347798739278
1103	38-39	0.2166192027750995
1103	40-41	0.2835918969027347
1103	42-43	0.3464896985852519
1103	44-45	0.2672623713214648
1103	46-47	0.31929091639435825
1103	48-49	0.28171813099522325
1103	50-51	0.23217637270614944
1103	52-53	0.2327608993059087
1103	54-55	0.1926902899418863
1103	56-57	0.16004509638013076
1103	58-59	0.20165608180208494
1103	60-61	0.21677229265089437
1103	62-63	0.19634710352256235
1103	64-65	0.23599103582768777
1103	66-67	0.24594130384467405
1103	68-69	0.21481153961833854
1103	70-71	0.25969426977826515
1103	72-73	0.27000115404830893
1103	74-75	0.20548414557287487
1103	76-77	0.19964347682611816
1103	78-79	0.22426934966330947
1103	80-81	0.2158917803404421
1103	82-83	0.20370688152591754
1103	84-85	0.2386614953742452
1103	86-87	0.20892415028606592
1103	88-89	0.2546922599600947
1103	90-91	0.27221066019336604
1103	92-93	0.2705637793863076
1103	94-95	0.16561175134143724
1103	96-97	0.2664377157640878
1103	98-99	0.2778934006369198
1103	100	0.33080271014642904
1104	1	0.1286469796635039
1104	2	0.1480453884558095
1104	3	0.2349249703331857
1104	4	0.13556478851619147
1104	5	0.14567195739440564
1104	6	0.1628881307483212
1104	7	0.15376191765678726
1104	8	0.21015075076967094
1104	9	0.2414956600785345
1104	10-11	0.18713614018181346
1104	12-13	0.14642652560810632
1104	14-15	0.19346396783115694
1104	16-17	0.24643547560370394
1104	18-19	0.2175647058449428
1104	20-21	0.23426281521567205
1104	22-23	0.2196621107906438
1104	24-25	0.20887014706035245
1104	26-27	0.20295636323096744
1104	28-29	0.30821525312617126
1104	30-31	0.32798895207478296
1104	32-33	0.25976196650799466
1104	34-35	0.2552475400504832
1104	36-37	0.27338330478686856
1104	38-39	0.22915203669966644
1104	40-41	0.21191994462586194
1104	42-43	0.2505619239033692
1104	44-45	0.17401371502576524
1104	46-47	0.25401173887558315
1104	48-49	0.21621634996007089
1104	50-51	0.18314147412831971
1104	52-53	0.1913120041085392
1104	54-55	0.16735635173224495
1104	56-57	0.1731332816627429
1104	58-59	0.26007761058085777
1104	60-61	0.26083814361908964
1104	62-63	0.2516854098651251
1104	64-65	0.1783483088254556
1104	66-67	0.20453375629064396
1104	68-69	0.19490305397906837
1104	70-71	0.27136012059589376
1104	72-73	0.25706931630647745
1104	74-75	0.2972259277522866
1104	76-77	0.24161130659051366
1104	78-79	0.207088669871915
1104	80-81	0.19936784491051895
1104	82-83	0.290774328622291
1104	84-85	0.301621582006689
1104	86-87	0.25890840731671716
1104	88-89	0.21710690398926147
1104	90-91	0.18315560910916417
1104	92-93	0.287245473860537
1104	94-95	0.24754839252387484
1104	96-97	0.2510071121468336
1104	98-99	0.2812125135427124
1104	100	0.29375896689929704
1105	1	0.09174629251878486
1105	2	0.06638231837927933
1105	3	0.20546855121168406
1105	4	0.12297991487501747
1105	5	0.0878383146463122
1105	6	0.11090688714490682
1105	7	0.0828018920540643
1105	8	0.09634534237676462
1105	9	0.11288618423163399
1105	10-11	0.15222834748083613
1105	12-13	0.12812045220708512
1105	14-15	0.14245531453358495
1105	16-17	0.13181481786279647
1105	18-19	0.11613205298136364
1105	20-21	0.1417657379382007
1105	22-23	0.12503427686026924
1105	24-25	0.144221163213885
1105	26-27	0.13761786808991872
1105	28-29	0.1612958081285143
1105	30-31	0.11596808833245831
1105	32-33	0.16524133851314105
1105	34-35	0.2129676053097569
1105	36-37	0.19350661800915248
1105	38-39	0.1854846143060982
1105	40-41	0.18077493644660336
1105	42-43	0.19700213707005787
1105	44-45	0.19640912415776768
1105	46-47	0.20573338719278667
1105	48-49	0.20588658748793165
1105	50-51	0.21003767569951037
1105	52-53	0.24722495121408627
1105	54-55	0.2013711075215241
1105	56-57	0.20678793546348118
1105	58-59	0.2049196925423331
1105	60-61	0.13141855207569364
1105	62-63	0.1566410219838943
1105	64-65	0.12318942595172189
1105	66-67	0.13524265895260612
1105	68-69	0.10291903237840927
1105	70-71	0.11615989544340266
1105	72-73	0.15823893324505178
1105	74-75	0.10326153636123792
1105	76-77	0.08708599277330364
1105	78-79	0.0989138031375063
1105	80-81	0.1342215340216839
1105	82-83	0.15556314075722355
1105	84-85	0.1535151166277018
1105	86-87	0.13786192883782178
1105	88-89	0.18780533181758585
1105	90-91	0.18439276451280762
1105	92-93	0.16917416489226156
1105	94-95	0.16634638624917386
1105	96-97	0.2301426234348618
1105	98-99	0.23514783972800757
1105	100	0.18633246640759538
1106	1	0.0916493679950463
1106	2	0.08967546441834529
1106	3	0.19908078850037825
1106	4	0.13718049275474442
1106	5	0.11827671911147064
1106	6	0.10066999836972457
1106	7	0.12027465931907244
1106	8	0.123981492953817
1106	9	0.20437769547706353
1106	10-11	0.17245806733404123
1106	12-13	0.14876550674240718
1106	14-15	0.25479192903855363
1106	16-17	0.16233180977963713
1106	18-19	0.18984992053706407
1106	20-21	0.18789534960266963
1106	22-23	0.19514371425269417
1106	24-25	0.1663086467650885
1106	26-27	0.22521589502095907
1106	28-29	0.23772743136979102
1106	30-31	0.24673651643497863
1106	32-33	0.21107428254752136
1106	34-35	0.1464501383754282
1106	36-37	0.13446273041016354
1106	38-39	0.2119173290397569
1106	40-41	0.1754528632397765
1106	42-43	0.2005748099428004
1106	44-45	0.14136638568196247
1106	46-47	0.16762537136003175
1106	48-49	0.23921399765593065
1106	50-51	0.23896750671029565
1106	52-53	0.1940384046872552
1106	54-55	0.23539406029658494
1106	56-57	0.216249122430888
1106	58-59	0.2431532736589901
1106	60-61	0.21322599360882322
1106	62-63	0.18670792610109288
1106	64-65	0.12817553780261903
1106	66-67	0.18569750565904997
1106	68-69	0.1785213397059877
1106	70-71	0.23591643151809905
1106	72-73	0.2921433499471533
1106	74-75	0.1883012797819461
1106	76-77	0.16485364635283162
1106	78-79	0.22018760094595535
1106	80-81	0.18954186084069846
1106	82-83	0.1828069216519168
1106	84-85	0.19706378539993352
1106	86-87	0.23918673863435913
1106	88-89	0.23408482721628587
1106	90-91	0.23764095852951073
1106	92-93	0.32913171893641646
1106	94-95	0.2878783874961002
1106	96-97	0.33079612175528084
1106	98-99	0.3468603388875593
1106	100	0.37833392289503465
1107	1	0.08191429226494407
1107	2	0.09238993853339039
1107	3	0.20606654536842584
1107	4	0.18753619100674968
1107	5	0.09392418660721802
1107	6	0.10256743149044212
1107	7	0.06853936529687843
1107	8	0.11008578362323362
1107	9	0.11741531875103561
1107	10-11	0.12852068607733713
1107	12-13	0.10470507570263976
1107	14-15	0.19677393070188032
1107	16-17	0.14313741008204062
1107	18-19	0.1531300734986658
1107	20-21	0.13931249742006457
1107	22-23	0.07229900277146584
1107	24-25	0.12038084473928023
1107	26-27	0.19830658650300848
1107	28-29	0.1540575159998241
1107	30-31	0.13252740081002656
1107	32-33	0.14121417737446507
1107	34-35	0.10638088273266533
1107	36-37	0.128291571988008
1107	38-39	0.06968719520006061
1107	40-41	0.09123714931787674
1107	42-43	0.07970594934382547
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2216	68-69	-0.4098545124015942
2216	70-71	-0.5233580851481605
2216	72-73	-0.5096523568746747
2216	74-75	-0.47541917467125216
2216	76-77	-0.43866291869002794
2216	78-79	-0.6141776987024699
2216	80-81	-0.7475919889835794
2216	82-83	-0.683832958031239
2216	84-85	-0.7562537736091031
2216	86-87	-0.5583649849918189
2216	88-89	-0.8440775516877181
2216	90-91	-0.6868610591711004
2216	92-93	-0.7663514993743838
2216	94-95	-0.7073346942678356
2216	96-97	-0.778808503781466
2216	98-99	-0.8505927827788717
2216	100	-0.7486703011196347
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	330.0
3	24.0
4	29.0
5	79.0
6	199.0
7	561.0
8	1381.0
9	2458.0
10	3558.0
11	4796.0
12	7061.0
13	10065.0
14	11910.0
15	15379.0
16	19617.0
17	27071.0
18	27002.0
19	14991.0
20	11349.0
21	11155.0
22	11989.0
23	13076.0
24	15603.0
25	19629.0
26	25089.0
27	32850.0
28	43935.0
29	57478.0
30	74649.0
31	97702.0
32	125991.0
33	165432.0
34	226047.0
35	327527.0
36	529561.0
37	972873.0
38	1420966.0
39	601274.0
40	2360.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.91880937697661	21.03602057066792	14.83557722122391	23.20959283113156
2	15.107501531508118	20.700171861361113	40.1163702912967	24.07595631583407
3	17.530649420256776	25.579935804369146	31.218237170300057	25.671177605074025
4	12.39261194507894	17.40817637142503	38.75015102237077	31.449060661125262
5	12.354516864428184	38.12954511269508	35.992954454509444	13.522983568367291
6	30.250701088130942	38.805557458819564	17.289155625145195	13.6545858279043
7	26.05953806228444	33.300338168344666	22.71774883104678	17.922374938324108
8	24.58896657668073	36.83183819640531	20.313529737423103	18.26566548949086
9	24.532611291279263	16.627556280642832	21.895011722980083	36.944820705097825
10-11	23.505943386702658	27.335413049057316	28.962511194908785	20.19613236933124
12-13	24.12511078076866	24.817842953937507	29.45310297693529	21.603943288358536
14-15	21.310820242523832	26.849026797060883	27.07848971861373	24.761663241801557
16-17	20.30699693455119	30.29181159064805	27.807879756239856	21.593311718560905
18-19	20.196987864493433	28.674165753606545	30.721744885765283	20.407101496134743
20-21	21.292831469759875	27.73756874690163	30.590018468325976	20.379581315012516
22-23	20.912008523739694	27.576825758365114	30.701700328762392	20.8094653891328
24-25	20.806571606960414	27.78807793976939	30.561915167928905	20.84343528534129
26-27	20.579780301846267	27.787383076686368	30.66163447658351	20.971202144883854
28-29	20.56635814075212	27.777934319029672	30.830745503724184	20.824962036494025
30-31	20.94233673701165	28.02400667092975	30.397905310218604	20.635751281839994
32-33	20.58721665133297	27.89000360731311	30.724070549273474	20.79870919208045
34-35	20.78201442436533	27.722863498830947	30.655222477327378	20.839899599476347
36-37	20.986091040956765	27.985857006936993	30.215464347719617	20.81258760438662
38-39	20.878087810605496	28.136898122977662	30.233937546649862	20.751076519766976
40-41	20.83043577723376	27.89798940267318	30.30397200588319	20.96760281420987
42-43	20.813868831021008	27.932998832256555	30.18792652004042	21.065205816682017
44-45	20.93857122296033	28.046768416166127	29.980367036081272	21.034293324792266
46-47	21.028594943483125	28.226306699317256	29.603368308385903	21.141730048813713
48-49	21.0919457454047	28.21901635343107	29.364363663785632	21.324674237378602
50-51	21.179334721732747	28.243538920439885	29.18165622123655	21.39547013659081
52-53	21.10925333244679	28.344650286680835	29.218497745393684	21.32759863547869
54-55	21.261205103567388	28.586484546991496	28.99674033350767	21.155570015933446
56-57	21.11676125047258	28.38428425618004	29.02493708208874	21.474017411258632
58-59	21.065154549729982	28.027889496132204	29.37565882295704	21.531297131180775
60-61	21.187183593715556	28.36195312967986	29.099217084649588	21.351646191954995
62-63	21.019582340570086	28.38593546855604	29.128365440801073	21.4661167500728
64-65	21.036089454330206	28.17716125213358	29.249780053598002	21.536969239938212
66-67	21.01091272214359	28.223370237492396	29.14140967752006	21.624307362843957
68-69	21.313646155845266	28.33084699501739	29.010307610627866	21.345199238509476
70-71	21.29651449715828	28.222557851749393	28.98959328857169	21.491334362520636
72-73	21.11558158215483	28.360542166384974	29.010868160521387	21.513008090938808
74-75	21.253938913132124	28.350454283166716	28.880132876774674	21.51547392692649
76-77	21.28814756379918	28.352877058446296	28.76132032069339	21.597655057061136
78-79	21.291378041586338	28.47200514102338	28.804367437212008	21.432249380178273
80-81	21.25242796287491	28.321001652073974	28.84298769656452	21.5835826884866
82-83	21.16734948722575	28.401034265361435	28.9084770432417	21.52313920417112
84-85	21.24502749814054	28.22968088209745	28.794726162956763	21.730565456805245
86-87	21.296188461460883	28.367618560516107	28.73624138713597	21.599951590887045
88-89	21.097261235777108	28.356527597709803	28.755813094289778	21.79039807222331
90-91	21.050049013004486	28.259871602247333	28.811193149146607	21.878886235601573
92-93	21.119500026201635	28.433283190400125	28.67105414798217	21.776162635416075
94-95	21.01191515750224	28.216069280248508	28.8261951622338	21.94582040001545
96-97	21.061956805550373	28.27173548143307	28.769905304351067	21.896402408665487
98-99	21.03935223922866	28.333452094475213	28.691347157805986	21.93584850849014
100	21.054782524082903	28.242718367876492	28.716409620187473	21.986089487853135
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	652.0
1	475.0
2	480.0
3	693.0
4	469.0
5	311.5
6	894.0
7	2483.0
8	4163.5
9	4819.5
10	3769.5
11	2369.5
12	2199.5
13	2481.0
14	2838.5
15	3241.5
16	3789.0
17	4440.0
18	5255.5
19	6246.0
20	7541.5
21	9685.5
22	12634.0
23	16702.0
24	22556.5
25	30387.0
26	40451.5
27	52294.5
28	65974.0
29	82342.5
30	99038.0
31	112744.0
32	123816.0
33	135063.5
34	146148.0
35	154626.0
36	161457.5
37	168732.0
38	175714.5
39	178871.0
40	181334.5
41	183473.0
42	182333.0
43	180537.5
44	177398.5
45	172249.0
46	167150.5
47	160691.0
48	153279.5
49	147813.0
50	143132.5
51	136601.5
52	127079.5
53	118121.5
54	110739.0
55	103900.0
56	95422.5
57	85486.0
58	77157.5
59	70059.5
60	61605.5
61	52031.0
62	43668.5
63	35983.0
64	28862.0
65	22659.0
66	17526.0
67	13345.0
68	10114.5
69	7522.0
70	5397.0
71	3907.5
72	2782.5
73	1848.5
74	1236.0
75	796.5
76	506.0
77	325.0
78	184.5
79	106.5
80	59.0
81	34.0
82	20.5
83	14.5
84	11.0
85	4.5
86	2.5
87	3.0
88	2.0
89	1.0
90	0.5
91	0.5
92	0.5
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03946851499053526
2	0.0
3	0.0
4	4.054290189063714E-5
5	0.0
6	0.0
7	0.0
8	0.0010743869001018842
9	0.0
10-11	0.0
12-13	0.04329981921920047
14-15	1.0135725472659284E-4
16-17	0.0
18-19	2.027145094531857E-5
20-21	1.1149298019925215E-4
22-23	0.0
24-25	1.2162870567191144E-4
26-27	1.0135725472659284E-4
28-29	3.851575679610529E-4
30-31	1.0135725472659286E-5
32-33	2.3312168587116358E-4
34-35	8.108580378127429E-5
36-37	5.067862736329642E-5
38-39	4.054290189063714E-4
40-41	1.9257878398052644E-4
42-43	5.574649009962607E-4
44-45	3.2434321512509715E-4
46-47	1.2162870567191144E-4
48-49	9.020795670666764E-4
50-51	5.574649009962607E-4
52-53	6.892293321408315E-4
54-55	5.270577245782829E-4
56-57	6.284149793048757E-4
58-59	1.2162870567191144E-4
60-61	1.9257878398052644E-4
62-63	0.0011250655274651809
64-65	5.270577245782829E-4
66-67	0.0017838876831880344
68-69	0.0018041591341333527
70-71	5.574649009962607E-4
72-73	0.0011858798803011364
74-75	0.0011047940765198621
76-77	9.628939199026322E-4
78-79	0.003567775366376069
80-81	0.003152210621997038
82-83	0.0015406302718442115
84-85	0.0033853323078682014
86-87	0.0018345663105513307
88-89	0.0022197238785123838
90-91	0.0034765538371221353
92-93	0.002990039014434489
94-95	0.004287411874934878
96-97	0.0038515756796105288
98-99	0.00355763964090341
100	0.0017433447812973972
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4933046.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.302182368675865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.19560301345885	47.47152354635741
2	14.435905222093401	17.987767996075664
3	4.467019546753114	8.34915199338757
4	1.808043259939716	4.505801636448776
5	0.9344534147631087	2.910924353080155
6	0.5237160102822069	1.9577190229198422
7	0.3507095339643919	1.5294978540438007
8	0.23111627748363894	1.1519238774524134
9	0.17092938186831352	0.9584346169192237
>10	0.826502194724273	8.965415330034489
>50	0.04144967481125503	1.7611356756920171
>100	0.013771257311489395	1.4414260241554848
>500	5.20808830747892E-4	0.23376398890485178
>1k	1.6275232214075822E-4	0.16521830618401248
>5k	3.2550464426834174E-5	0.13751630467711679
>10k+	6.510092885366835E-5	0.4727794736672502
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11975	0.2427506250701899	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11338	0.22983771081802198	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6781	0.13746070886020523	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.027145094531857E-5	0.0
5	0.0	0.0	0.0	2.027145094531857E-5	0.0
6	0.0	0.0	0.0	2.027145094531857E-5	0.0
7	0.0	0.0	0.0	1.2162870567191143E-4	0.0
8	0.0	0.0	0.0	1.2162870567191143E-4	0.0
9	2.027145094531857E-5	0.0	0.0	2.0271450945318572E-4	0.0
10-11	2.027145094531857E-5	0.0	0.0	2.12850234925845E-4	0.0
12-13	2.027145094531857E-5	0.0	0.0	2.4325741134382286E-4	0.0
14-15	4.054290189063714E-5	0.0	0.0	2.4325741134382286E-4	0.0
16-17	4.054290189063714E-5	0.0	0.0	2.4325741134382286E-4	0.0
18-19	6.0814352835955715E-5	0.0	0.0	2.4325741134382286E-4	0.0
20-21	6.0814352835955715E-5	0.0	0.0	2.736645877618007E-4	0.0
22-23	6.0814352835955715E-5	0.0	0.0	3.7502184248839357E-4	0.0
24-25	6.0814352835955715E-5	0.0	0.0	5.777363519415793E-4	0.0
26-27	6.0814352835955715E-5	0.0	0.0	9.933010963206102E-4	0.0
28-29	6.0814352835955715E-5	0.0	0.0	0.002858274583289919	0.0
30-31	6.0814352835955715E-5	0.0	0.0	0.00723690798747873	0.0
32-33	6.0814352835955715E-5	0.0	0.0	0.018122677145114806	0.0
34-35	1.0135725472659286E-4	0.0	0.0	0.03433983790136966	0.0
36-37	1.0135725472659286E-4	0.0	0.0	0.06122991758033475	0.0
38-39	1.0135725472659286E-4	0.0	0.0	0.09680631398936884	0.0
40-41	1.0135725472659286E-4	0.0	0.0	0.13359899745512205	0.0
42-43	1.0135725472659286E-4	0.0	0.0	0.16810100696405428	0.0
44-45	1.0135725472659286E-4	0.0	0.0	0.21278942057300904	0.0
46-47	1.0135725472659286E-4	0.0	0.0	0.26495799958078636	0.0
48-49	1.0135725472659286E-4	0.0	0.0	0.3186570731349353	0.0
50-51	1.0135725472659286E-4	0.0	0.0	0.37237641814002953	0.0
52-53	1.0135725472659286E-4	1.0135725472659286E-5	0.0	0.4254673481658188	0.0
54-55	1.0135725472659286E-4	3.0407176417977858E-5	0.0	0.48396061986853556	0.0
56-57	1.1149298019925215E-4	4.054290189063714E-5	0.0	0.5531470819449078	0.0
58-59	1.2162870567191143E-4	4.054290189063714E-5	0.0	0.628820408323782	0.0
60-61	1.4190015661723002E-4	4.054290189063714E-5	0.0	0.7056593431320122	0.0
62-63	1.4190015661723002E-4	5.067862736329643E-5	0.0	0.7799947537484954	0.0
64-65	1.4190015661723002E-4	6.0814352835955715E-5	0.0	0.8603913281976288	0.0
66-67	1.4190015661723002E-4	7.095007830861501E-5	0.0	0.9520892365487774	0.0
68-69	1.4190015661723002E-4	8.108580378127429E-5	0.0	1.0515409748865103	0.0
70-71	1.4190015661723002E-4	8.108580378127429E-5	0.0	1.1560403045096277	0.0
72-73	1.520358820898893E-4	8.108580378127429E-5	0.0	1.2659115686332543	0.0
74-75	1.6217160756254857E-4	8.108580378127429E-5	0.0	1.3744854598963805	0.0
76-77	1.6217160756254857E-4	8.108580378127429E-5	0.0	1.4915429533801225	0.0
78-79	1.6217160756254857E-4	8.108580378127429E-5	0.0	1.6222228618991186	0.0
80-81	1.6217160756254857E-4	8.108580378127429E-5	0.0	1.7637175894974424	0.0
82-83	1.6217160756254857E-4	8.108580378127429E-5	0.0	1.9074827196016417	0.0
84-85	1.6217160756254857E-4	8.108580378127429E-5	0.0	2.0527378013503217	0.0
86-87	1.6217160756254857E-4	1.0135725472659286E-4	0.0	2.206740014181907	0.0
88	1.6217160756254857E-4	1.0135725472659286E-4	0.0	2.3334872612175115	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8100	0.0	56.068665	1
GTATCAA	14420	0.0	40.00437	1
ATCAACG	17515	0.0	32.358887	3
TCAACGC	17565	0.0	32.320286	4
CAACGCA	18115	0.0	31.287104	5
TATCAAC	18370	0.0	31.134209	2
AACGCAG	18835	0.0	30.14722	6
GTGGTAT	3640	0.0	27.123518	1
ACGCAGA	21790	0.0	25.821388	7
TGGTATC	3590	0.0	25.526812	2
CGCAGAG	22125	0.0	25.239054	8
GTACATG	13640	0.0	23.300243	1
GCAGAGT	24505	0.0	22.327013	9
TACATGG	13735	0.0	22.308754	2
GAGTACT	16885	0.0	21.497957	12-13
ACATGGG	14255	0.0	20.703741	3
CAGAGTA	23810	0.0	20.242224	10-11
AGAGTAC	22425	0.0	19.971476	10-11
GTACTTT	18490	0.0	19.443989	14-15
AGTACTT	17780	0.0	19.00098	12-13
>>END_MODULE