FastQCFastQC Report
Wed 25 May 2016
SRR2049372_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049372_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4814256
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50000.047.482261
GTATCAA83700.034.48681
TCAACGC92700.030.4761184
ATCAACG95400.029.7614063
CAACGCA97950.028.8903245
AACGCAG103850.027.3361056
TATCAAC105750.026.8041362
GTACATG135450.023.185021
ACGCAGA120300.022.8946327
TACATGG135800.022.0844082
CGCAGAG123050.022.07748
GTGGTAT31200.021.0951921
ACATGGG141950.020.2003693
GCAGAGT139400.018.5439229
TGGTATC31900.017.8303362
CAGAGTA130050.017.32925810-11
AGAGTAC119500.016.99094410-11
GTATAGG16700.016.8905891
CATGGGG98200.016.7541144
GTACTAA15400.015.87422851
Produced by FastQC (version 0.11.5)