##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049372_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4814256
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.60139676826492	31.0	31.0	34.0	27.0	34.0
2	30.82980215426849	31.0	31.0	34.0	27.0	34.0
3	30.8790132888654	31.0	31.0	34.0	27.0	34.0
4	34.49452604930025	37.0	35.0	37.0	32.0	37.0
5	34.52997617908146	37.0	35.0	37.0	32.0	37.0
6	34.50733155860428	37.0	35.0	37.0	32.0	37.0
7	34.46131094815066	37.0	35.0	37.0	32.0	37.0
8	34.42314970371331	37.0	35.0	37.0	31.0	37.0
9	35.98378981092821	39.0	37.0	39.0	31.0	39.0
10-11	35.95164195672187	39.0	37.0	39.0	31.0	39.0
12-13	35.89926626253361	39.0	37.0	39.0	31.0	39.0
14-15	37.083030482799415	40.0	37.0	41.0	31.0	41.0
16-17	37.044549978231316	40.0	37.0	41.0	31.0	41.0
18-19	37.064506436716286	40.0	37.0	41.0	31.0	41.0
20-21	37.148919583836005	40.0	38.0	41.0	31.0	41.0
22-23	37.08662366521432	40.0	37.0	41.0	31.0	41.0
24-25	37.08855875134185	40.0	37.0	41.0	31.0	41.0
26-27	37.071598913726234	40.0	37.0	41.0	31.0	41.0
28-29	37.009158424479295	40.0	37.0	41.0	31.0	41.0
30-31	36.93754694390992	40.0	37.0	41.0	31.0	41.0
32-33	36.8800625683387	40.0	37.0	41.0	30.0	41.0
34-35	36.77228028588426	40.0	37.0	41.0	30.0	41.0
36-37	36.6818787783616	40.0	37.0	41.0	30.0	41.0
38-39	36.57496267336012	40.0	37.0	41.0	30.0	41.0
40-41	36.474034305612335	40.0	37.0	41.0	30.0	41.0
42-43	36.32759579050221	40.0	36.0	41.0	30.0	41.0
44-45	36.19434352888588	40.0	36.0	41.0	29.5	41.0
46-47	36.082213741853366	40.0	36.0	41.0	29.0	41.0
48-49	35.96142768477621	39.5	36.0	41.0	29.0	41.0
50-51	35.827154912410144	39.0	35.0	41.0	28.0	41.0
52-53	35.68701342429651	39.0	35.0	41.0	28.0	41.0
54-55	35.555461113825274	39.0	35.0	41.0	28.0	41.0
56-57	35.42957873449189	39.0	35.0	41.0	28.0	41.0
58-59	35.23714837765171	39.0	35.0	40.0	27.0	41.0
60-61	35.02061138834328	38.5	34.5	40.0	27.0	41.0
62-63	34.80824742182385	38.0	34.0	40.0	26.0	41.0
64-65	34.558111055997024	38.0	34.0	40.0	26.0	41.0
66-67	34.28421037435483	37.0	34.0	40.0	26.0	41.0
68-69	33.969922974598774	37.0	34.0	40.0	25.5	41.0
70-71	33.64910123184143	36.0	34.0	39.0	25.0	41.0
72-73	33.262129496229534	36.0	33.0	39.0	24.0	41.0
74-75	32.8580310228621	35.5	33.0	39.0	22.5	40.0
76-77	32.46433644160177	35.0	33.0	37.5	22.0	40.0
78-79	32.080014959736246	35.0	32.5	37.0	20.5	39.0
80-81	31.638253242037813	35.0	32.0	37.0	18.5	39.0
82-83	31.215118909339264	35.0	32.0	36.0	13.5	38.5
84-85	30.830997666098355	35.0	32.0	36.0	7.0	37.0
86-87	30.420519183026414	35.0	31.0	35.5	2.0	37.0
88-89	30.072018293169286	35.0	31.0	35.0	2.0	36.5
90-91	29.760722321372192	35.0	31.0	35.0	2.0	36.0
92-93	29.408770534844844	34.5	31.0	35.0	2.0	36.0
94-95	29.09440783788814	34.0	30.5	35.0	2.0	36.0
96-97	28.73963848204167	34.0	30.0	35.0	2.0	35.0
98-99	28.429504060440493	34.0	30.0	35.0	2.0	35.0
100	28.24254962760601	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.20935057965166948
1101	2	0.23428552789916424
1101	3	0.19854307447437947
1101	4	0.2486230828572218
1101	5	0.23253802374593846
1101	6	0.36396740959083473
1101	7	0.2914858694960998
1101	8	0.35461941334313707
1101	9	0.413480110580835
1101	10-11	0.4222173540622052
1101	12-13	0.412791755407639
1101	14-15	0.5053517144006534
1101	16-17	0.5413635387719395
1101	18-19	0.4963922743063094
1101	20-21	0.4169020002402206
1101	22-23	0.500084002326858
1101	24-25	0.5233650379035737
1101	26-27	0.4668652942923188
1101	28-29	0.44444793624945333
1101	30-31	0.4968957944502961
1101	32-33	0.39669178621659995
1101	34-35	0.4829961986971725
1101	36-37	0.4911532540293919
1101	38-39	0.5600817855857088
1101	40-41	0.5823491370297731
1101	42-43	0.573202193808342
1101	44-45	0.45597532935433094
1101	46-47	0.5333233582754389
1101	48-49	0.41942574589400294
1101	50-51	0.3679615148665789
1101	52-53	0.36725146157471755
1101	54-55	0.382031904597234
1101	56-57	0.3687680342819064
1101	58-59	0.45249007757846726
1101	60-61	0.4926798539880721
1101	62-63	0.5050736702213285
1101	64-65	0.43678612150169016
1101	66-67	0.43781206182724475
1101	68-69	0.37073291468373526
1101	70-71	0.4004629787066136
1101	72-73	0.4742018362931262
1101	74-75	0.4472792378097452
1101	76-77	0.4697781921695423
1101	78-79	0.4033234971051769
1101	80-81	0.3945181049804667
1101	82-83	0.35804424490364894
1101	84-85	0.3456735270314759
1101	86-87	0.4039133764040912
1101	88-89	0.41472611813520643
1101	90-91	0.4652812847534129
1101	92-93	0.45568819556596907
1101	94-95	0.3998041103232026
1101	96-97	0.4619700562149731
1101	98-99	0.5688154228261908
1101	100	0.6367287372146926
1102	1	0.08259692754732484
1102	2	0.16568189329946037
1102	3	0.210132792062538
1102	4	0.21313254139866444
1102	5	0.21989778634132762
1102	6	0.27272965049198916
1102	7	0.3954343893937704
1102	8	0.34824276398356346
1102	9	0.4137634991717647
1102	10-11	0.35458773467562565
1102	12-13	0.3414426689961729
1102	14-15	0.4397783908128474
1102	16-17	0.438790729897363
1102	18-19	0.3910203144341864
1102	20-21	0.41233572569943533
1102	22-23	0.42797375492429524
1102	24-25	0.49015509307336913
1102	26-27	0.46915363905579
1102	28-29	0.48641607231811435
1102	30-31	0.42928980358343694
1102	32-33	0.44414686404365256
1102	34-35	0.4888602419725956
1102	36-37	0.4585565093436159
1102	38-39	0.4426711662507046
1102	40-41	0.5304692265394024
1102	42-43	0.39243864033358733
1102	44-45	0.39971065481442025
1102	46-47	0.29303336291773974
1102	48-49	0.3152232386150402
1102	50-51	0.36040082754334435
1102	52-53	0.363198660119032
1102	54-55	0.31969599550283334
1102	56-57	0.33394082537747494
1102	58-59	0.39918606863189154
1102	60-61	0.3329884185308103
1102	62-63	0.362916857185148
1102	64-65	0.2957699963813809
1102	66-67	0.41983481895613295
1102	68-69	0.4587538258688184
1102	70-71	0.44105987045331574
1102	72-73	0.43964423176189626
1102	74-75	0.4000314280846311
1102	76-77	0.4333095090122967
1102	78-79	0.42051944041867984
1102	80-81	0.3747093171026741
1102	82-83	0.33025158543857813
1102	84-85	0.4170817231536823
1102	86-87	0.4485645402156919
1102	88-89	0.39210391215553386
1102	90-91	0.4036265969670403
1102	92-93	0.3631493314250136
1102	94-95	0.34837094676840863
1102	96-97	0.3980233800661708
1102	98-99	0.4351266191009415
1102	100	0.41904626369388964
1103	1	0.1722172477733679
1103	2	0.2491279392478596
1103	3	0.20957399425568113
1103	4	0.18943291308960397
1103	5	0.18860393185580193
1103	6	0.3003763086372402
1103	7	0.3270271103060125
1103	8	0.303904827961496
1103	9	0.4196303715859031
1103	10-11	0.39881656006497934
1103	12-13	0.3925500052671538
1103	14-15	0.4337191136760481
1103	16-17	0.41663689862150477
1103	18-19	0.4241341721152381
1103	20-21	0.44427226073256065
1103	22-23	0.3989085744607692
1103	24-25	0.4454220251935226
1103	26-27	0.4690723421739591
1103	28-29	0.4173504293432444
1103	30-31	0.47416107826472853
1103	32-33	0.5204367451361875
1103	34-35	0.5719645340779422
1103	36-37	0.512276803500832
1103	38-39	0.5125963865742946
1103	40-41	0.4791201650169725
1103	42-43	0.5092675363610368
1103	44-45	0.36228104063297195
1103	46-47	0.3953028010084907
1103	48-49	0.4740887192904708
1103	50-51	0.43572532130365005
1103	52-53	0.44128039428432686
1103	54-55	0.4129697980188993
1103	56-57	0.3732689014984416
1103	58-59	0.4724383853933105
1103	60-61	0.4385203065862413
1103	62-63	0.5023039665732867
1103	64-65	0.4722786663669112
1103	66-67	0.4196153573193442
1103	68-69	0.5181508582277914
1103	70-71	0.44321019084753743
1103	72-73	0.5250194756315167
1103	74-75	0.5673643143236546
1103	76-77	0.5500376616578606
1103	78-79	0.41074186633277066
1103	80-81	0.42764367833244776
1103	82-83	0.40696449623274944
1103	84-85	0.4757600338940371
1103	86-87	0.41650920283468196
1103	88-89	0.45578871907806473
1103	90-91	0.5613574742249376
1103	92-93	0.44612306918948974
1103	94-95	0.49901312275818555
1103	96-97	0.4875673859190428
1103	98-99	0.3872587964362033
1103	100	0.39520313072608104
1104	1	0.05632942691242704
1104	2	0.12126747334976073
1104	3	0.10754855553669529
1104	4	0.16166855031180205
1104	5	0.1967725354545138
1104	6	0.19488559763577484
1104	7	0.2734408120960623
1104	8	0.22541673401641305
1104	9	0.31357962474214673
1104	10-11	0.3250096546048482
1104	12-13	0.27412982031471245
1104	14-15	0.40736348752203355
1104	16-17	0.459905445586287
1104	18-19	0.3820029100578992
1104	20-21	0.3462065097770335
1104	22-23	0.3763010669742357
1104	24-25	0.3767334271030407
1104	26-27	0.2964604869344214
1104	28-29	0.31186392063093393
1104	30-31	0.24260759217494865
1104	32-33	0.3117374085806688
1104	34-35	0.3184582086630243
1104	36-37	0.2854056372815492
1104	38-39	0.37875016385038407
1104	40-41	0.3609193072327841
1104	42-43	0.33432834025248326
1104	44-45	0.2840783790969823
1104	46-47	0.3266484677372148
1104	48-49	0.3163754337209994
1104	50-51	0.3139684379540171
1104	52-53	0.3122600157679827
1104	54-55	0.31762106943764223
1104	56-57	0.30012927106714216
1104	58-59	0.3747284551900947
1104	60-61	0.31124040309666157
1104	62-63	0.2684367970542354
1104	64-65	0.33408008428392577
1104	66-67	0.34714178971808707
1104	68-69	0.38477367562345677
1104	70-71	0.3459823925108694
1104	72-73	0.32032023346588545
1104	74-75	0.374011414485544
1104	76-77	0.39043358841131237
1104	78-79	0.3141693826635077
1104	80-81	0.33681289546537485
1104	82-83	0.23455176484824491
1104	84-85	0.2787502535509212
1104	86-87	0.32893794170831825
1104	88-89	0.260811957737058
1104	90-91	0.2866407408388518
1104	92-93	0.28650994485862213
1104	94-95	0.2869328021429176
1104	96-97	0.32101304967384436
1104	98-99	0.34553750001060735
1104	100	0.3872126395366635
1105	1	0.048018281765070725
1105	2	0.03265140528711541
1105	3	0.06307101345838362
1105	4	0.0835518641496904
1105	5	0.07710597065039337
1105	6	0.11926689037918692
1105	7	0.1420186632394902
1105	8	0.19771116016560342
1105	9	0.2398612617563458
1105	10-11	0.20385102276963352
1105	12-13	0.1861939193989599
1105	14-15	0.22998255822171387
1105	16-17	0.20242001023776623
1105	18-19	0.16590892401030288
1105	20-21	0.1410820005098401
1105	22-23	0.20798690441822743
1105	24-25	0.17688809430025287
1105	26-27	0.17757377839639332
1105	28-29	0.24244584581457929
1105	30-31	0.19541824832053578
1105	32-33	0.23342292135703246
1105	34-35	0.2805833060248446
1105	36-37	0.2086366872320511
1105	38-39	0.23606731827246819
1105	40-41	0.27171510606441274
1105	42-43	0.27396670359526354
1105	44-45	0.25866848236591267
1105	46-47	0.19074378984598184
1105	48-49	0.21757941977784157
1105	50-51	0.2580457271608694
1105	52-53	0.22994078793531258
1105	54-55	0.22729976542472485
1105	56-57	0.22903648444331992
1105	58-59	0.3136638015550659
1105	60-61	0.23367065110272733
1105	62-63	0.24082206474501078
1105	64-65	0.2602514920212329
1105	66-67	0.2833299824641813
1105	68-69	0.26037615719203444
1105	70-71	0.2482793429124257
1105	72-73	0.28687729403285545
1105	74-75	0.26338172700044993
1105	76-77	0.3022221239202594
1105	78-79	0.22279850590395256
1105	80-81	0.2440304069295287
1105	82-83	0.22409808260953312
1105	84-85	0.21868229876927714
1105	86-87	0.2175250897324439
1105	88-89	0.11981646061551743
1105	90-91	0.12489815503196766
1105	92-93	0.16664319399379224
1105	94-95	0.20717112049383957
1105	96-97	0.18078325269420503
1105	98-99	0.2117748591302444
1105	100	0.2847306478192273
1106	1	0.06366174364286792
1106	2	0.043473577585281475
1106	3	0.07930592347706167
1106	4	0.08661228167585477
1106	5	0.05884905472287727
1106	6	0.11972762313652652
1106	7	0.12195923339015025
1106	8	0.1338599613325897
1106	9	0.2340527036026998
1106	10-11	0.13661959537694202
1106	12-13	0.19688459692032723
1106	14-15	0.24272554520407397
1106	16-17	0.22291035177383378
1106	18-19	0.24727101096713255
1106	20-21	0.24331063470555137
1106	22-23	0.2631913103208987
1106	24-25	0.32636300963959286
1106	26-27	0.2279722599008025
1106	28-29	0.32336644360475475
1106	30-31	0.2433432696499338
1106	32-33	0.29034833891510914
1106	34-35	0.33115913660939356
1106	36-37	0.3152320020542234
1106	38-39	0.28438641567866085
1106	40-41	0.3429461569730421
1106	42-43	0.2922255308919617
1106	44-45	0.2648063712849975
1106	46-47	0.261571374844749
1106	48-49	0.288212935855384
1106	50-51	0.28331300708879326
1106	52-53	0.37446241787969115
1106	54-55	0.2873131019567836
1106	56-57	0.26733577635341277
1106	58-59	0.3348743024614649
1106	60-61	0.37858799013834954
1106	62-63	0.29751954688910587
1106	64-65	0.3393006181432412
1106	66-67	0.33588920187733606
1106	68-69	0.37530583959001973
1106	70-71	0.38262601243143024
1106	72-73	0.42753860772766217
1106	74-75	0.4754559871446915
1106	76-77	0.4090010229686385
1106	78-79	0.31527147469007843
1106	80-81	0.4122572642570681
1106	82-83	0.3520316074905132
1106	84-85	0.3711744124229206
1106	86-87	0.3922848955759335
1106	88-89	0.34928618445347936
1106	90-91	0.3894465693823612
1106	92-93	0.42816745116149946
1106	94-95	0.40483382403239077
1106	96-97	0.3405307010720229
1106	98-99	0.3627089285473133
1106	100	0.3758322927249864
1107	1	0.1303465056874451
1107	2	0.06910559623229773
1107	3	0.11328446223862443
1107	4	0.12041634732852913
1107	5	0.14597052616927186
1107	6	0.2060065413238945
1107	7	0.17804286938521585
1107	8	0.22874636985216057
1107	9	0.22314789983895622
1107	10-11	0.28974351889733896
1107	12-13	0.2996032140342777
1107	14-15	0.26837275855795184
1107	16-17	0.2903106898267538
1107	18-19	0.25637946672681977
1107	20-21	0.19409693598746003
1107	22-23	0.2662578185898852
1107	24-25	0.22678697352289845
1107	26-27	0.26225502645203136
1107	28-29	0.28580421744764095
1107	30-31	0.23152578251718836
1107	32-33	0.25345525698850224
1107	34-35	0.2354815212170891
1107	36-37	0.22900895710350255
1107	38-39	0.2000418242548605
1107	40-41	0.24687677965483346
1107	42-43	0.2735947552350879
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2216	76-77	-3.503280840559526
2216	78-79	-2.4255067175895135
2216	80-81	-2.261767832923045
2216	82-83	-2.5091525365483633
2216	84-85	-2.4440269099313703
2216	86-87	-2.094030234464988
2216	88-89	-1.97509162111524
2216	90-91	-2.136188388721049
2216	92-93	-2.1703515696951996
2216	94-95	-1.9729100378281252
2216	96-97	-2.2664510057389293
2216	98-99	-1.951790646676514
2216	100	-1.555099203416205
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	104953.0
3	12969.0
4	8288.0
5	6986.0
6	6566.0
7	7189.0
8	8510.0
9	9557.0
10	10393.0
11	11871.0
12	12906.0
13	14146.0
14	15842.0
15	16046.0
16	16170.0
17	16748.0
18	16320.0
19	15299.0
20	15546.0
21	16769.0
22	18820.0
23	22264.0
24	27206.0
25	33829.0
26	41701.0
27	50131.0
28	62054.0
29	78444.0
30	95454.0
31	113703.0
32	140492.0
33	181313.0
34	243207.0
35	340459.0
36	543899.0
37	926494.0
38	1169687.0
39	381319.0
40	706.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.34692630226192	20.79096437215894	14.760987018566482	23.10112230701266
2	15.257259053531378	20.335573175257483	40.91054166384418	23.496626107366957
3	18.149519582419043	25.638755901323222	30.63875590132323	25.572968614934506
4	12.804634393012835	17.179612444354305	39.05518843429855	30.96056472833431
5	13.043305693346808	37.8781691901324	35.90939420821652	13.169130908304277
6	30.40016823640207	38.31958100491327	17.829068823151456	13.451181935533207
7	26.50031190478862	32.805505649404054	22.853457114442254	17.84072533136507
8	24.57620268378473	36.68380093604354	20.693469326942598	18.046527053229138
9	24.625285330900727	17.25039195660373	21.90944425800459	36.21487845449096
10-11	23.572861039657568	27.296128126394194	29.199160164850063	19.93185066909818
12-13	23.985559565286643	24.85653231117919	29.16151366061342	21.996394462920748
14-15	21.504153916149505	27.113221267660027	26.332485971357457	25.050138844833015
16-17	21.064890066362775	30.409610264801756	27.044355268113403	21.48114440072207
18-19	20.935449420568858	29.434420471713334	28.989146971424347	20.640983136293464
20-21	22.048868075355113	28.57315177433944	28.54798796542397	20.829992184881476
22-23	21.775418875540062	28.427390196342454	28.481812015938797	21.315378912178684
24-25	21.575033093186736	28.5928977517412	28.414270906265525	21.41779824880653
26-27	21.343521998529884	28.56484754664249	28.4917260384984	21.599904416329224
28-29	21.33809826937736	28.543870966468376	28.676774406903334	21.441256357250932
30-31	21.612653172202112	28.845916684129808	28.275477081481178	21.265953062186902
32-33	21.367201067298243	28.603536750090985	28.53685658392875	21.492405598682023
34-35	21.53646770287243	28.31517464167203	28.602196497452326	21.546161158003216
36-37	21.679785406962846	28.510780299687628	28.23152634689722	21.57790794645231
38-39	21.491000781275975	28.54148658532531	28.461405798065094	21.50610683533362
40-41	21.415064416078735	28.224905167800348	28.714819152597894	21.645211263523024
42-43	21.176667131766724	28.2915240241496	28.74363037158101	21.788178472502665
44-45	21.243967957658096	28.30364964534999	28.66713058467799	21.785251812313927
46-47	21.210881747249925	28.4016316886028	28.59765540344128	21.789831160705994
48-49	21.11151883068796	28.31816436427468	28.59158813101069	21.978728674026673
50-51	21.110052899388723	28.230354048875387	28.5187620522806	22.14083099945529
52-53	21.051673568506708	28.200140235886778	28.61984012610217	22.128346069504346
54-55	21.091252014491875	28.412688452130517	28.478930846099054	22.01712868727855
56-57	21.14999157959434	28.152631566371028	28.418957413525654	22.278419440508976
58-59	21.04999380801866	27.862399214075495	28.551953216879756	22.53565376102609
60-61	21.24449699051937	28.044827158101754	28.239426559789887	22.471249291588986
62-63	21.165354006761508	28.03830752635198	28.240284240590512	22.556054226295995
64-65	21.28349001571845	27.79406646629883	28.259573659074782	22.662869858907936
66-67	21.248842400443323	27.876919298506614	28.04018317136463	22.834055129685435
68-69	21.594878327234397	27.94772482821321	27.865422174072087	22.591974670480308
70-71	21.580570606692714	27.730850690479937	27.928111377745434	22.760467325081915
72-73	21.45254098938861	27.786046814115817	28.021793132709323	22.73961906378625
74-75	21.563088277461258	27.79233623809939	27.956420197889788	22.68815528654956
76-77	21.61219341916073	27.81445573849517	27.79049859124494	22.78285225109916
78-79	21.76012405391849	27.844529921171823	27.853028188065608	22.54231783684408
80-81	21.81981309591883	27.66225905597149	27.883453403635688	22.634474444473994
82-83	21.706500930696098	27.674259170164202	27.99381357466778	22.62542632447192
84-85	21.780335338772364	27.514630424009585	27.9320637183146	22.772970518903453
86-87	22.039897039897042	27.562740793286483	27.801264062161707	22.59609810465477
88-89	22.103681056932242	27.30519803662003	27.848954884357518	22.742166022090206
90-91	22.34893803761469	27.171309873430555	27.757842073483168	22.721910015471583
92-93	22.511746641764802	27.22816862720205	27.571038521878876	22.689046209154274
94-95	22.45144521545874	26.93238401332472	27.694501723354087	22.92166904786245
96-97	22.54396110219315	26.9074806159041	27.6799468079803	22.86861147392245
98-99	22.665080544117306	26.886200069128023	27.532218893220467	22.916500493534205
100	22.775585677205367	26.802043763356163	27.51511344639753	22.907257113040934
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	902.0
1	761.0
2	779.5
3	980.5
4	966.5
5	873.0
6	760.0
7	681.0
8	689.5
9	679.5
10	654.5
11	609.0
12	589.5
13	666.5
14	768.0
15	862.5
16	1049.0
17	1332.0
18	1647.5
19	2230.5
20	3142.5
21	4513.0
22	6502.0
23	9424.0
24	13626.0
25	19481.5
26	27542.0
27	37578.0
28	49773.5
29	63893.0
30	78244.5
31	91255.0
32	102212.0
33	113496.5
34	125355.0
35	135656.5
36	145876.5
37	156896.0
38	167207.0
39	173968.5
40	178874.0
41	183529.5
42	186084.0
43	187051.5
44	185748.5
45	182812.0
46	179792.0
47	174696.0
48	168715.5
49	163332.5
50	157535.0
51	150537.5
52	140961.5
53	131307.0
54	123025.0
55	114812.0
56	104489.5
57	92851.5
58	82437.0
59	73049.5
60	62370.0
61	51296.5
62	42451.0
63	34275.5
64	27054.0
65	21569.5
66	16986.0
67	12881.0
68	9692.0
69	7498.5
70	5687.0
71	4200.5
72	3161.5
73	2307.5
74	1650.5
75	1226.0
76	936.5
77	743.0
78	553.0
79	393.0
80	324.5
81	271.5
82	202.0
83	160.5
84	128.5
85	97.5
86	78.0
87	62.0
88	52.0
89	39.0
90	33.0
91	36.0
92	36.0
93	28.5
94	28.0
95	38.0
96	41.5
97	34.5
98	38.5
99	64.0
100	126.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014311661033397476
2	0.035602593630251485
3	0.036890435406841685
4	0.039237630902885096
5	0.04206257415476036
6	0.04112785028465458
7	0.039258402544443
8	0.038905284637958594
9	0.03865602493926372
10-11	0.03911300105353766
12-13	0.04020351223532774
14-15	0.03883258389250592
16-17	0.0351767749783144
18-19	0.037814773456168516
20-21	0.03716046674709446
22-23	0.03838599359901094
24-25	0.03550912124324091
26-27	0.035696066017262065
28-29	0.0361322704899781
30-31	0.0361322704899781
32-33	0.03594532571595695
34-35	0.03594532571595695
36-37	0.03347350037056608
38-39	0.03356697275757666
40-41	0.034823657071829994
42-43	0.03471979886404047
44-45	0.033733145890039916
46-47	0.03267379217058669
48-49	0.03281919366149204
50-51	0.033847389918608396
52-53	0.03428359439132443
54-55	0.033816232456271536
56-57	0.03204025710307055
58-59	0.03281919366149204
60-61	0.03216488695241798
62-63	0.03266340634980774
64-65	0.029931935484943054
66-67	0.031053604129069996
68-69	0.03181176904593358
70-71	0.03300613843551319
72-73	0.030233124287532692
74-75	0.030658942939469778
76-77	0.0310743757706279
78-79	0.03136517875243859
80-81	0.03157289516801765
82-83	0.030336982495322226
84-85	0.0313859503939965
86-87	0.03153135188490184
88-89	0.03188446979138625
90-91	0.03244530411344972
92-93	0.03110553323296476
94-95	0.032154501131639035
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4814256.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.92019062200953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.07581981167533	51.3361729576564
2	12.614888070395565	16.379218764107883
3	3.8297002164628955	7.458746042337669
4	1.5608839961964056	4.053315462876586
5	0.8107863997539537	2.631820381287975
6	0.5004498218285672	1.9493578697916774
7	0.31537553675482344	1.4331967974549196
8	0.22496093137058387	1.1683605237666488
9	0.16557054626021384	0.9673984282142997
>10	0.8493330961159281	9.661003572797508
>50	0.04092488854147369	1.793644343048767
>100	0.01121069062207558	1.104842933047973
>500	6.399601572664086E-5	0.03223995625271971
>1k	3.199800647198786E-5	0.03068196735895848
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.077164155790635E-5	0.0
10-11	0.0	0.0	0.0	8.30865662316254E-5	0.0
12-13	0.0	0.0	0.0	1.4540149090534445E-4	0.0
14-15	0.0	0.0	0.0	1.4540149090534445E-4	0.0
16-17	0.0	0.0	0.0	2.2848805713696985E-4	0.0
18-19	0.0	0.0	0.0	2.804171610317357E-4	0.0
20-21	0.0	0.0	0.0	4.881335766107992E-4	0.0
22-23	0.0	0.0	0.0	0.001786361173979946	0.0
24-25	0.0	0.0	0.0	0.004123170849244411	0.0
26-27	0.0	0.0	0.0	0.008703317812762762	0.0
28-29	0.0	0.0	0.0	0.02671233104346757	0.0
30-31	0.0	0.0	0.0	0.07985657596937097	0.0
32-33	2.077164155790635E-5	0.0	0.0	0.20236771787790264	0.0
34-35	2.077164155790635E-5	0.0	0.0	0.3936122217015464	0.0
36-37	2.077164155790635E-5	0.0	0.0	0.6894834840523645	0.0
38-39	2.077164155790635E-5	0.0	0.0	1.065772156694617	0.0
40-41	2.077164155790635E-5	0.0	0.0	1.4322670003423168	0.0
42-43	2.077164155790635E-5	0.0	0.0	1.7901935418473798	0.0
44-45	2.077164155790635E-5	0.0	0.0	2.2209662302960207	0.0
46-47	2.077164155790635E-5	0.0	0.0	2.7177927388988037	0.0
48-49	2.077164155790635E-5	0.0	0.0	3.2115554303717957	0.0
50-51	2.077164155790635E-5	0.0	0.0	3.6889189108348206	0.0
52-53	2.077164155790635E-5	0.0	0.0	4.130804427516941	0.0
54-55	2.077164155790635E-5	0.0	0.0	4.608136750517629	0.0
56-57	2.077164155790635E-5	0.0	0.0	5.145873007168709	0.0
58-59	2.077164155790635E-5	0.0	0.0	5.703363094941357	0.0
60-61	2.077164155790635E-5	0.0	0.0	6.2473100724182515	0.0
62-63	2.077164155790635E-5	0.0	0.0	6.764243530049088	0.0
64-65	2.077164155790635E-5	0.0	0.0	7.290970816674477	0.0
66-67	2.077164155790635E-5	0.0	0.0	7.868235507210253	0.0
68-69	2.077164155790635E-5	0.0	0.0	8.473811945189453	0.0
70-71	2.077164155790635E-5	0.0	0.0	9.084051616698407	0.0
72-73	2.077164155790635E-5	0.0	0.0	9.679325320464887	0.0
74-75	2.077164155790635E-5	0.0	0.0	10.258345214712303	0.0
76-77	2.077164155790635E-5	0.0	0.0	10.858645655735799	0.0
78-79	2.077164155790635E-5	0.0	0.0	11.497051673197271	0.0
80-81	3.1157462336859525E-5	0.0	0.0	12.151223366601194	0.0
82-83	4.15432831158127E-5	2.077164155790635E-5	0.0	12.791332658670417	0.0
84-85	4.15432831158127E-5	2.077164155790635E-5	1.0385820778953175E-5	13.40720144504156	0.0
86-87	4.15432831158127E-5	2.077164155790635E-5	2.077164155790635E-5	14.037122246926629	0.0
88	6.231492467371905E-5	2.077164155790635E-5	2.077164155790635E-5	14.5237394937037	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5000	0.0	47.48226	1
GTATCAA	8370	0.0	34.4868	1
TCAACGC	9270	0.0	30.476118	4
ATCAACG	9540	0.0	29.761406	3
CAACGCA	9795	0.0	28.890324	5
AACGCAG	10385	0.0	27.336105	6
TATCAAC	10575	0.0	26.804136	2
GTACATG	13545	0.0	23.18502	1
ACGCAGA	12030	0.0	22.894632	7
TACATGG	13580	0.0	22.084408	2
CGCAGAG	12305	0.0	22.0774	8
GTGGTAT	3120	0.0	21.095192	1
ACATGGG	14195	0.0	20.200369	3
GCAGAGT	13940	0.0	18.543922	9
TGGTATC	3190	0.0	17.830336	2
CAGAGTA	13005	0.0	17.329258	10-11
AGAGTAC	11950	0.0	16.990944	10-11
GTATAGG	1670	0.0	16.890589	1
CATGGGG	9820	0.0	16.754114	4
GTACTAA	1540	0.0	15.8742285	1
>>END_MODULE