FastQCFastQC Report
Wed 25 May 2016
SRR2049372_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049372_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4814256
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT162200.33691602606924104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105830.21982628260732293No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104170.21637819010871048No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT86820.18033939200574295TruSeq Adapter, Index 11 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56750.11787906584111854No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76900.055.7560041
GTATCAA139050.040.40361
ATCAACG170250.032.6283873
TCAACGC170750.032.6154144
CAACGCA174550.031.8784395
TATCAAC180150.031.017932
AACGCAG181500.030.763236
GTGGTAT36500.030.5266131
TGGTATC37000.028.4519162
ACGCAGA210750.026.404327
CGCAGAG212550.025.826628
GTACATG130300.023.7773861
GCAGAGT229500.023.3042749
TACATGG130350.022.7861672
ACATGGG130400.021.6601833
GAGTACT154650.021.29706212-13
CAGAGTA221300.020.7987510-11
AGAGTAC212150.020.04394710-11
GTACTTT169600.019.30014214-15
AGTACTT162300.018.65644312-13
Produced by FastQC (version 0.11.5)