##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049372_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4814256
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.703087455257883	31.0	31.0	34.0	30.0	34.0
2	31.958968945565005	33.0	31.0	34.0	30.0	34.0
3	31.907484562515993	33.0	31.0	34.0	30.0	34.0
4	35.63730698990664	37.0	35.0	37.0	33.0	37.0
5	35.74617967968467	37.0	35.0	37.0	33.0	37.0
6	35.803808522022926	37.0	35.0	37.0	35.0	37.0
7	35.75288746589297	37.0	35.0	37.0	33.0	37.0
8	35.7404055787644	37.0	35.0	37.0	33.0	37.0
9	37.474401652093285	39.0	37.0	39.0	34.0	39.0
10-11	37.4365080502574	39.0	37.0	39.0	34.0	39.0
12-13	37.386381509417035	39.0	37.0	39.0	34.0	39.0
14-15	38.65876181075539	40.0	38.0	41.0	34.0	41.0
16-17	38.676525718615714	40.0	38.0	41.0	34.0	41.0
18-19	38.746478999039525	40.0	38.0	41.0	34.0	41.0
20-21	38.783655875383445	40.0	38.0	41.0	34.0	41.0
22-23	38.750228799631756	40.0	38.0	41.0	34.0	41.0
24-25	38.72922119222575	40.0	38.0	41.0	34.0	41.0
26-27	38.610940922127945	40.0	38.0	41.0	34.0	41.0
28-29	38.525760470569075	40.0	38.0	41.0	34.0	41.0
30-31	38.423666086722434	40.0	38.0	41.0	34.0	41.0
32-33	38.29130669411847	40.0	38.0	41.0	34.0	41.0
34-35	38.190529543921215	40.0	38.0	41.0	34.0	41.0
36-37	38.0715224740853	40.0	38.0	41.0	33.0	41.0
38-39	37.96836427061627	40.0	37.5	41.0	33.0	41.0
40-41	37.88212830809164	40.0	37.0	41.0	33.0	41.0
42-43	37.75860631424669	40.0	37.0	41.0	33.0	41.0
44-45	37.59584180816309	40.0	37.0	41.0	33.0	41.0
46-47	37.37928134689971	40.0	37.0	41.0	32.0	41.0
48-49	37.17948785856008	40.0	37.0	41.0	31.5	41.0
50-51	37.032040360961275	40.0	37.0	41.0	31.0	41.0
52-53	36.90548851162049	40.0	36.0	41.0	31.0	41.0
54-55	36.75468940579811	39.5	36.0	41.0	31.0	41.0
56-57	36.60879271895803	39.0	36.0	41.0	31.0	41.0
58-59	36.41108491114723	39.0	35.0	41.0	30.5	41.0
60-61	36.20313928050357	39.0	35.0	41.0	30.0	41.0
62-63	35.97920696365129	39.0	35.0	41.0	30.0	41.0
64-65	35.716557553233564	38.0	35.0	40.0	29.5	41.0
66-67	35.393577117627316	38.0	35.0	40.0	29.0	41.0
68-69	35.039634369256646	37.0	34.5	40.0	28.5	41.0
70-71	34.66586768547414	37.0	34.0	39.5	28.0	41.0
72-73	34.35510512943225	36.0	34.0	39.0	28.0	41.0
74-75	34.03235754392787	36.0	34.0	39.0	28.0	40.5
76-77	33.68909848167608	35.0	34.0	38.5	27.5	40.0
78-79	33.34041999428365	35.0	34.0	37.0	27.0	39.0
80-81	33.005986283238784	35.0	33.0	37.0	26.5	39.0
82-83	32.69588478468947	35.0	33.0	36.0	26.0	39.0
84-85	32.423099851773564	35.0	33.0	36.0	26.0	37.0
86-87	32.1872172979584	35.0	33.0	36.0	26.0	37.0
88-89	31.950318076147177	35.0	33.0	35.0	26.0	36.5
90-91	31.7471907393375	35.0	33.0	35.0	25.0	36.0
92-93	31.561216000977097	35.0	33.0	35.0	25.0	36.0
94-95	31.39926096576501	35.0	33.0	35.0	25.0	36.0
96-97	31.19881524788046	35.0	33.0	35.0	24.0	35.5
98-99	31.0353512567674	35.0	33.0	35.0	23.5	35.0
100	30.911713461020767	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.21008021158297296
1101	2	0.19477719254212644
1101	3	0.4051770508027026
1101	4	0.24544545135308482
1101	5	0.19504167817758855
1101	6	0.21986509395254927
1101	7	0.06948691066403967
1101	8	0.15308291008093278
1101	9	0.2766992598137463
1101	10-11	0.29592938371683886
1101	12-13	0.20554362913454582
1101	14-15	0.36497643241590794
1101	16-17	0.2658742789149571
1101	18-19	0.3588233781602952
1101	20-21	0.29534418763952175
1101	22-23	0.32660764549474663
1101	24-25	0.35299898680745656
1101	26-27	0.410762068671815
1101	28-29	0.4403158429868341
1101	30-31	0.3934111277506318
1101	32-33	0.43009801056891206
1101	34-35	0.3572705560343934
1101	36-37	0.3606894065734991
1101	38-39	0.36866084338657146
1101	40-41	0.3452223569379811
1101	42-43	0.32738631843827903
1101	44-45	0.3789190114751122
1101	46-47	0.37051176787849016
1101	48-49	0.4383248821191472
1101	50-51	0.36549169002977777
1101	52-53	0.41047176021142917
1101	54-55	0.33785693632403735
1101	56-57	0.37769490265938543
1101	58-59	0.37321189088331863
1101	60-61	0.43190356850364253
1101	62-63	0.35254393914674864
1101	64-65	0.3354178247359627
1101	66-67	0.4011477096067253
1101	68-69	0.3500205276241459
1101	70-71	0.36618681904563033
1101	72-73	0.37154820729417537
1101	74-75	0.44940388075004023
1101	76-77	0.4061816670888305
1101	78-79	0.4585094107526828
1101	80-81	0.473582088800363
1101	82-83	0.46695299531848633
1101	84-85	0.45858417428186726
1101	86-87	0.41828702523030614
1101	88-89	0.5497443886694455
1101	90-91	0.5187382225889507
1101	92-93	0.5520162460800258
1101	94-95	0.5728194579586159
1101	96-97	0.6184385268078252
1101	98-99	0.6875358147889692
1101	100	0.6631199990851044
1102	1	0.16915480329685906
1102	2	0.22539450819742513
1102	3	0.3839873937948646
1102	4	0.22582974129576172
1102	5	0.13935727969516876
1102	6	0.13621778555152275
1102	7	0.12925455440785782
1102	8	0.2166781984245958
1102	9	0.14745551511590094
1102	10-11	0.22539555257131383
1102	12-13	0.2006122923178495
1102	14-15	0.30112072551929714
1102	16-17	0.2579691836930067
1102	18-19	0.30126328347062525
1102	20-21	0.20335634101748212
1102	22-23	0.2558326234630357
1102	24-25	0.2671085054902278
1102	26-27	0.369231208376398
1102	28-29	0.36771785816045366
1102	30-31	0.2579793996860289
1102	32-33	0.3321869670619222
1102	34-35	0.3361996342587332
1102	36-37	0.36151193806264814
1102	38-39	0.32713257311932153
1102	40-41	0.29612872412715774
1102	42-43	0.3396328715607453
1102	44-45	0.34861553919115806
1102	46-47	0.27045787114818864
1102	48-49	0.21789773598997186
1102	50-51	0.3157476089575013
1102	52-53	0.38672575637248485
1102	54-55	0.25237626111686495
1102	56-57	0.3430246319026722
1102	58-59	0.350289581851257
1102	60-61	0.3752344764292417
1102	62-63	0.36965719910326555
1102	64-65	0.3749747652981128
1102	66-67	0.39062187901431855
1102	68-69	0.4318124978783331
1102	70-71	0.43278988735104207
1102	72-73	0.45780546293983093
1102	74-75	0.4267677478815983
1102	76-77	0.37721976354982445
1102	78-79	0.40792864083388736
1102	80-81	0.4576762319345349
1102	82-83	0.5007897050169348
1102	84-85	0.464549250605387
1102	86-87	0.44288763959377064
1102	88-89	0.44395039687632476
1102	90-91	0.4538685525870676
1102	92-93	0.4980230372216887
1102	94-95	0.48962685314753074
1102	96-97	0.5192280683618584
1102	98-99	0.5438190365645212
1102	100	0.5644024581193108
1103	1	0.21045265219860454
1103	2	0.19621908184604564
1103	3	0.3531248101223561
1103	4	0.2706255635568553
1103	5	0.20678575862147142
1103	6	0.1355029532329155
1103	7	0.19760637417979865
1103	8	0.17798197588095377
1103	9	0.22120892088130972
1103	10-11	0.24229975385625835
1103	12-13	0.24398038408010336
1103	14-15	0.3276526472119201
1103	16-17	0.3256765149184133
1103	18-19	0.36688464644953456
1103	20-21	0.3802845087997824
1103	22-23	0.2825429904447745
1103	24-25	0.36721936105214326
1103	26-27	0.3539091743355627
1103	28-29	0.39620431078247265
1103	30-31	0.3567294837790058
1103	32-33	0.3789393805302623
1103	34-35	0.39314340128755987
1103	36-37	0.4065830176482379
1103	38-39	0.4161091195891018
1103	40-41	0.4288049619757146
1103	42-43	0.4473379953455989
1103	44-45	0.4321437439743647
1103	46-47	0.36491807835327705
1103	48-49	0.28010593337300094
1103	50-51	0.3293013359580783
1103	52-53	0.32519303579661596
1103	54-55	0.24429147858128886
1103	56-57	0.2311293654961304
1103	58-59	0.30071828782858034
1103	60-61	0.30471795636465515
1103	62-63	0.31809220784894165
1103	64-65	0.31458877855116185
1103	66-67	0.3161953930914905
1103	68-69	0.30439028387853284
1103	70-71	0.3312093599199173
1103	72-73	0.305003737352024
1103	74-75	0.27830475719909487
1103	76-77	0.30469823574868116
1103	78-79	0.3059409562498061
1103	80-81	0.3483035865377886
1103	82-83	0.26651710923503913
1103	84-85	0.24595145888687142
1103	86-87	0.26567174770369917
1103	88-89	0.35350771044220863
1103	90-91	0.3550068560954287
1103	92-93	0.3462936021582834
1103	94-95	0.3481508112355911
1103	96-97	0.36349347419455214
1103	98-99	0.3717751568511112
1103	100	0.46380325363883657
1104	1	0.1315367328929007
1104	2	0.15230370560540507
1104	3	0.30209560485928577
1104	4	0.14399378235518157
1104	5	0.1595922735919615
1104	6	0.17001328310615094
1104	7	0.19258964906898512
1104	8	0.16262939735633353
1104	9	0.15558338751384326
1104	10-11	0.14126734838836796
1104	12-13	0.15127756221061617
1104	14-15	0.2553991340981909
1104	16-17	0.2508507159662656
1104	18-19	0.20309719435562812
1104	20-21	0.23516944365783132
1104	22-23	0.2650210609604784
1104	24-25	0.23028817321368678
1104	26-27	0.26826118781391983
1104	28-29	0.3166593411165337
1104	30-31	0.353582777339291
1104	32-33	0.2803481818046478
1104	34-35	0.24612195151073024
1104	36-37	0.2749140655827844
1104	38-39	0.3080247880504672
1104	40-41	0.353183394922695
1104	42-43	0.4206102241621821
1104	44-45	0.3302948013996172
1104	46-47	0.37574091242878893
1104	48-49	0.3458523633667383
1104	50-51	0.31782892495609616
1104	52-53	0.30526885155791206
1104	54-55	0.26306213444872384
1104	56-57	0.2672458587698827
1104	58-59	0.2926191836273304
1104	60-61	0.2979883943595567
1104	62-63	0.3648548774274687
1104	64-65	0.3320454109271509
1104	66-67	0.4246108973403224
1104	68-69	0.3224536621569669
1104	70-71	0.34627715254146807
1104	72-73	0.33058565742319246
1104	74-75	0.33853956172153943
1104	76-77	0.36726967466420746
1104	78-79	0.3280305315987988
1104	80-81	0.30384409466566353
1104	82-83	0.29273648424473464
1104	84-85	0.32247368964296896
1104	86-87	0.3184290635658513
1104	88-89	0.31292612332163827
1104	90-91	0.2655136941896359
1104	92-93	0.35422340259480833
1104	94-95	0.44724547652649704
1104	96-97	0.5003095031476761
1104	98-99	0.49539745251641776
1104	100	0.4764813983734335
1105	1	0.20478432781355949
1105	2	0.16852204627991796
1105	3	0.2892905826972587
1105	4	0.21664062675506557
1105	5	0.15365520279564748
1105	6	0.15140671195639044
1105	7	0.1653436107065076
1105	8	0.18796366653256769
1105	9	0.23410175370015196
1105	10-11	0.22976514741878873
1105	12-13	0.21806867582673561
1105	14-15	0.28369970754431506
1105	16-17	0.23519311937457132
1105	18-19	0.16765826064414568
1105	20-21	0.2327532856114729
1105	22-23	0.2819918304104547
1105	24-25	0.3140071262944488
1105	26-27	0.30182676236638173
1105	28-29	0.26833656486669355
1105	30-31	0.3313681063322278
1105	32-33	0.32276804660135383
1105	34-35	0.3282090650677816
1105	36-37	0.32279936758676087
1105	38-39	0.34911332322089805
1105	40-41	0.31498695977329305
1105	42-43	0.299494291505944
1105	44-45	0.357407106702901
1105	46-47	0.3698497721006717
1105	48-49	0.42012214201111675
1105	50-51	0.36547805433907143
1105	52-53	0.3603404061496178
1105	54-55	0.3488101063531204
1105	56-57	0.3417946438513013
1105	58-59	0.39203616288275356
1105	60-61	0.3976778405381296
1105	62-63	0.45606036218701007
1105	64-65	0.34082222716558164
1105	66-67	0.39869470759656167
1105	68-69	0.3446962799689999
1105	70-71	0.37200460037384886
1105	72-73	0.37245477974656893
1105	74-75	0.3635175812612985
1105	76-77	0.2928065572148455
1105	78-79	0.3686630510780802
1105	80-81	0.35974126288689234
1105	82-83	0.3429431598112984
1105	84-85	0.3508639698326732
1105	86-87	0.2870882124073475
1105	88-89	0.3598575218205369
1105	90-91	0.36363400324243855
1105	92-93	0.4519658239136781
1105	94-95	0.4245152225502409
1105	96-97	0.438794815553333
1105	98-99	0.5269607048490208
1105	100	0.581687097629036
1106	1	0.1689726179420319
1106	2	0.15874292484465968
1106	3	0.2666310969394452
1106	4	0.1444865886592126
1106	5	0.1156042218661355
1106	6	0.14242030769238312
1106	7	0.16442897416133206
1106	8	0.14275421832666524
1106	9	0.2018872035117596
1106	10-11	0.1455707824765824
1106	12-13	0.17753092936589354
1106	14-15	0.21546408760972469
1106	16-17	0.18894253167493957
1106	18-19	0.20511543669601906
1106	20-21	0.21540432571548962
1106	22-23	0.24538081563895275
1106	24-25	0.19682538707765218
1106	26-27	0.24375147995508684
1106	28-29	0.23756392931944248
1106	30-31	0.2963548477394937
1106	32-33	0.19073422657528027
1106	34-35	0.21209761836395558
1106	36-37	0.24593317352437083
1106	38-39	0.30434978074344343
1106	40-41	0.2978688116235446
1106	42-43	0.3124446713228295
1106	44-45	0.2607900854073506
1106	46-47	0.3291200536576042
1106	48-49	0.3302418805684795
1106	50-51	0.28594941579360267
1106	52-53	0.3158353995500178
1106	54-55	0.28611309615019564
1106	56-57	0.35128501864147665
1106	58-59	0.3414612787043083
1106	60-61	0.28996582219016886
1106	62-63	0.32900443411447355
1106	64-65	0.26697139427625416
1106	66-67	0.24627505895891488
1106	68-69	0.3123964790037732
1106	70-71	0.4259215785132753
1106	72-73	0.37590196189849223
1106	74-75	0.3332683268437364
1106	76-77	0.3193828095624909
1106	78-79	0.37888076679075056
1106	80-81	0.307930520799097
1106	82-83	0.3091787675725044
1106	84-85	0.2829488846538979
1106	86-87	0.39334944587660203
1106	88-89	0.3669461406154326
1106	90-91	0.3711443480178076
1106	92-93	0.33247748890700635
1106	94-95	0.2866850734218751
1106	96-97	0.3272138334020305
1106	98-99	0.35064938682673485
1106	100	0.3211043104029514
1107	1	0.14038225750013922
1107	2	0.11232779354976685
1107	3	0.24577753856453555
1107	4	0.18639607906571598
1107	5	0.11953545774678531
1107	6	0.0939916297527148
1107	7	0.06357911662185245
1107	8	0.07969604054444801
1107	9	0.17169055063966
1107	10-11	0.16885908296710284
1107	12-13	0.1280386309631325
1107	14-15	0.19506826414318823
1107	16-17	0.15423211302965711
1107	18-19	0.16660760380207762
1107	20-21	0.08685021614696353
1107	22-23	0.09878880352790986
1107	24-25	0.07699724146673503
1107	26-27	0.2220902755676164
1107	28-29	0.19961503619747845
1107	30-31	0.19603981682886484
1107	32-33	0.18860954665606755
1107	34-35	0.14460108924981085
1107	36-37	0.1370179024562148
1107	38-39	0.18494765503552202
1107	40-41	0.26344481996594027
1107	42-43	0.30934649313662277
1107	44-45	0.19791955549172258
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2216	82-83	-1.0778119488036992
2216	84-85	-1.1897727889496679
2216	86-87	-0.9739274403291134
2216	88-89	-1.3399172346499206
2216	90-91	-1.2082330600058384
2216	92-93	-1.2667612632616532
2216	94-95	-1.254045457136236
2216	96-97	-1.3411188334729722
2216	98-99	-1.4858428764803229
2216	100	-1.4611842123691012
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	248.0
3	27.0
4	38.0
5	96.0
6	203.0
7	623.0
8	1501.0
9	2681.0
10	3899.0
11	5530.0
12	7550.0
13	10132.0
14	12351.0
15	16044.0
16	20469.0
17	27532.0
18	27858.0
19	17309.0
20	14477.0
21	15380.0
22	17274.0
23	20089.0
24	23260.0
25	28658.0
26	33211.0
27	36959.0
28	46070.0
29	60193.0
30	78487.0
31	100504.0
32	129815.0
33	168441.0
34	227662.0
35	326536.0
36	523638.0
37	952820.0
38	1343897.0
39	511230.0
40	1564.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.44696073804814	21.09196115260488	15.243511044144785	23.21756706520219
2	15.16803427154684	20.707104067585934	40.38227298257509	23.74258867829214
3	17.521191228717374	25.70029512348325	31.562073142765986	25.216440505033383
4	12.425333691436657	18.013573445350712	38.25536381241285	31.30572905079978
5	12.410765027867235	37.97733232300069	36.106534426087855	13.505368223044226
6	29.962739829373426	38.66794786151796	17.345587770986835	14.02372453812178
7	25.787432159818675	33.389250592407215	22.51303212791343	18.31028511986068
8	24.430910951680946	36.767382689938515	20.39356665418284	18.4081397041977
9	24.31297795547225	16.720880651132802	21.608655626123745	37.3574857672712
10-11	23.424543688578257	27.24728390014989	28.961640178669352	20.366532232602506
12-13	24.136352070092002	24.7206391186382	29.15471221571802	21.988296595551777
14-15	21.518349598597663	26.77377168225893	26.626074648849553	25.081804070293863
16-17	20.65351323236654	30.28204150340156	27.369940443549325	21.69450482068257
18-19	20.487847383959895	28.51121300995596	30.21203815380231	20.788901452281834
20-21	21.472100687760367	27.595983548422243	30.081324202245852	20.850591561571537
22-23	21.14438866566298	27.672302843886985	30.110197712793003	21.073110777657025
24-25	21.152970747990782	27.839141315974313	30.05197797663416	20.955909959400742
26-27	20.959511168242386	27.783933687746188	30.12621790688837	21.130337237123058
28-29	20.672592283017106	27.552308890356297	30.46400479909701	21.311094027529585
30-31	21.17329240489081	27.79807513352011	30.20670275947104	20.82192970211804
32-33	20.812431368977098	27.8293118094121	30.293023375130513	21.065233446480292
34-35	20.906899215153015	27.615513567480544	30.51328550518923	20.964301712177214
36-37	21.315184854300284	27.645961957529842	29.86076131053408	21.178091877635794
38-39	21.06467424522134	27.50740121628688	30.15637454905938	21.271549989432398
40-41	20.887153251451966	27.322092171736713	30.388234574439192	21.40252000237213
42-43	20.94798733172546	27.284596439182085	30.334481249445655	21.432934979646802
44-45	20.870631076305134	27.46964294597895	30.108022163814525	21.551703813901398
46-47	21.119831167974677	27.44092670669019	29.65376244806437	21.785479677270768
48-49	21.03312026973469	27.48592085267537	29.466920027618226	22.01403884997171
50-51	20.847322663655042	27.534938316777048	29.45848400197373	22.15925501759418
52-53	20.802652138894558	27.61689180662941	29.628481412646163	21.951974641829867
54-55	20.789861568896914	27.913442548308808	29.226735041655623	22.069960841138656
56-57	20.92442830155895	27.719440878337853	29.01863112259189	22.337499697511305
58-59	20.575344329513594	27.642149681751928	29.370561027450293	22.41194496128419
60-61	20.85800488134185	28.18667497533365	28.65931349639092	22.296006646933584
62-63	20.49941309178181	28.33142336303914	28.63652008605356	22.53264345912549
64-65	20.501915881512176	28.068240569162317	28.720608246422643	22.709235302902872
66-67	20.59266003230251	28.09630058224911	28.481870159932853	22.829169225515518
68-69	20.891669336734402	28.132519140101444	28.24385706993258	22.73195445323158
70-71	20.862131191212722	27.84530036719249	28.281963947524346	23.010604494070446
72-73	20.67867438645418	27.82566438491395	28.382671834840558	23.112989393791317
74-75	20.82567284705456	27.728855448051988	28.3019093279537	23.14356237693975
76-77	20.87035003128757	27.758122434354526	28.097876859403275	23.273650674954627
78-79	20.935161901802328	27.75149662692245	28.137001590440363	23.176339880834856
80-81	20.856969557229107	27.576491213857583	28.18236581386195	23.384173415051364
82-83	20.70028216596444	27.50837732136759	28.32856755905669	23.46277295361128
84-85	20.7504831628346	27.38085445457023	28.247192836394337	23.621469546200835
86-87	20.886610433454106	27.484487225491787	28.161684939950803	23.467217401103305
88-89	20.771927069523123	27.2502509011722	28.219010368712294	23.75881166059238
90-91	20.734297263067983	27.008848774426358	28.271885184248895	23.984968778256764
92-93	20.736871875143134	27.079359563185008	28.211092763465313	23.97267579820655
94-95	20.577543874882284	26.812781772386025	28.508087303052093	24.101587049679598
96-97	20.65170698714774	26.84627601942296	28.447511225368167	24.054505768061127
98-99	20.657149777279614	26.8700868364399	28.379348780708703	24.093414605571787
100	20.69654717578817	26.803969527489137	28.319597854253324	24.179885442469374
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	346.0
1	280.5
2	316.0
3	429.5
4	312.0
5	234.0
6	582.5
7	1675.5
8	2948.0
9	3532.5
10	2866.0
11	1943.5
12	1908.0
13	2084.0
14	2332.5
15	2767.0
16	3229.0
17	3681.5
18	4212.5
19	4888.0
20	5779.5
21	7024.5
22	8971.5
23	11812.0
24	15766.0
25	21137.0
26	28671.5
27	38459.0
28	49989.5
29	63660.0
30	77670.5
31	89997.0
32	100890.5
33	111836.5
34	123652.0
35	134376.0
36	144309.5
37	155173.0
38	165691.5
39	173285.5
40	179113.5
41	185731.0
42	189991.0
43	191386.5
44	191268.0
45	189512.5
46	186849.5
47	180647.0
48	172859.0
49	165989.0
50	159384.5
51	151329.5
52	139693.5
53	128804.0
54	119550.5
55	110330.0
56	99394.0
57	87801.5
58	77392.5
59	68071.5
60	58575.0
61	47840.5
62	38934.5
63	31387.0
64	24499.0
65	18992.0
66	14641.5
67	10698.5
68	7641.5
69	5664.5
70	4003.5
71	2769.5
72	1883.0
73	1175.5
74	735.0
75	463.5
76	299.5
77	176.5
78	99.5
79	52.0
80	27.5
81	14.0
82	8.5
83	6.5
84	7.5
85	4.5
86	0.5
87	0.5
88	1.5
89	1.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	1.0
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03919608761976929
2	0.0
3	0.0
4	6.231492467371905E-5
5	0.0
6	0.0
7	0.0
8	5.608343220634715E-4
9	0.0
10-11	0.0
12-13	0.04292459727941348
14-15	1.8694477402115718E-4
16-17	0.0
18-19	9.347238701057859E-5
20-21	1.3501567012639128E-4
22-23	0.0
24-25	1.1424402856848494E-4
26-27	5.192910389476588E-5
28-29	3.53117906484408E-4
30-31	0.0
32-33	3.219604441475484E-4
34-35	1.1424402856848494E-4
36-37	1.0385820778953176E-4
38-39	2.804171610317357E-4
40-41	3.011888025896421E-4
42-43	5.192910389476588E-4
44-45	2.596455194738294E-4
46-47	7.270074545267223E-5
48-49	9.139522285478795E-4
50-51	4.465902934949866E-4
52-53	5.91991784400331E-4
54-55	4.1543283115812705E-4
56-57	5.504485012845183E-4
58-59	1.76558953242204E-4
60-61	1.246298493474381E-4
62-63	9.347238701057859E-4
64-65	4.777477558318461E-4
66-67	0.001651345503853555
68-69	0.0017344320700851802
70-71	4.881335766107993E-4
72-73	9.866529740005516E-4
74-75	0.0010178104363374112
76-77	9.035664077689262E-4
78-79	0.003749281301202097
80-81	0.0029288014596647954
82-83	0.001412471625937632
84-85	0.002991116384338515
86-87	0.0016305738622956487
88-89	0.0022744947505907455
90-91	0.002970344742780608
92-93	0.0028145574310963106
94-95	0.004102399207686505
96-97	0.0033234626492650164
98-99	0.0030742029505701402
100	0.0016825029661904144
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4814256.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.933435732641406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.71512925357979	48.13164963080504
2	13.308925205075859	16.48534927718194
3	4.049716178622256	7.524385100524188
4	1.7254086180354302	4.274419350305719
5	0.9177388548994803	2.8419360194632435
6	0.5235624117246797	1.9455611387146312
7	0.3538154293041768	1.5339103606419016
8	0.24966115266883615	1.236989836300203
9	0.18848485120300118	1.0506162976701798
>10	0.9057503271378007	9.849435570949455
>50	0.04575432168775867	1.9148505090018153
>100	0.015013257022195282	1.4915862741123407
>500	5.703992303532911E-4	0.24190527262860298
>1k	3.019755911293946E-4	0.4056131885767142
>5k	6.710568691757632E-5	0.2983446154205007
>10k+	1.0065853037636446E-4	0.7734475577036314
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT	16220	0.33691602606924104	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10583	0.21982628260732293	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10417	0.21637819010871048	No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	8682	0.18033939200574295	TruSeq Adapter, Index 11 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5675	0.11787906584111854	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.077164155790635E-5	0.0
5	0.0	0.0	0.0	2.077164155790635E-5	0.0
6	0.0	0.0	0.0	2.077164155790635E-5	0.0
7	0.0	0.0	0.0	4.15432831158127E-5	0.0
8	0.0	0.0	0.0	4.15432831158127E-5	0.0
9	0.0	0.0	0.0	8.30865662316254E-5	0.0
10-11	0.0	0.0	0.0	1.661731324632508E-4	0.0
12-13	0.0	1.0385820778953175E-5	0.0	2.804171610317357E-4	0.0
14-15	0.0	2.077164155790635E-5	0.0	3.1157462336859525E-4	0.0
16-17	0.0	2.077164155790635E-5	0.0	4.2581865193708023E-4	0.0
18-19	0.0	2.077164155790635E-5	0.0	4.881335766107992E-4	0.0
20-21	0.0	2.077164155790635E-5	0.0	7.062358129688159E-4	0.0
22-23	0.0	2.077164155790635E-5	0.0	0.002004463410337963	0.0
24-25	0.0	2.077164155790635E-5	0.0	0.004320501444044521	0.0
26-27	0.0	2.077164155790635E-5	0.0	0.008931805869899731	0.0
28-29	0.0	2.077164155790635E-5	0.0	0.027055063129173024	0.0
30-31	0.0	2.077164155790635E-5	0.0	0.08039663864987653	0.0
32-33	0.0	2.077164155790635E-5	0.0	0.20389443353240877	0.0
34-35	0.0	2.077164155790635E-5	0.0	0.39612559033005307	0.0
36-37	0.0	2.077164155790635E-5	0.0	0.6932846944574613	0.0
38-39	0.0	2.077164155790635E-5	0.0	1.0712870275282411	0.0
40-41	0.0	2.077164155790635E-5	0.0	1.4395370748875838	0.0
42-43	0.0	2.077164155790635E-5	0.0	1.7991980484627323	0.0
44-45	0.0	2.077164155790635E-5	0.0	2.2310612480931633	0.0
46-47	0.0	2.077164155790635E-5	0.0	2.7300999365218637	0.0
48-49	0.0	2.077164155790635E-5	0.0	3.226562941397383	0.0
50-51	0.0	2.077164155790635E-5	0.0	3.7063359322811253	0.0
52-53	0.0	2.077164155790635E-5	0.0	4.149976652674889	0.0
54-55	0.0	2.077164155790635E-5	0.0	4.629012250283325	0.0
56-57	0.0	2.077164155790635E-5	0.0	5.168742584523963	0.0
58-59	0.0	2.077164155790635E-5	0.0	5.729618449870551	0.0
60-61	0.0	2.077164155790635E-5	0.0	6.277013519846057	0.0
62-63	0.0	2.077164155790635E-5	0.0	6.798111691609254	0.0
64-65	0.0	2.077164155790635E-5	0.0	7.32865057446052	0.0
66-67	0.0	2.077164155790635E-5	0.0	7.910308467185792	0.0
68-69	0.0	2.077164155790635E-5	0.0	8.520693540185649	0.0
70-71	0.0	2.077164155790635E-5	0.0	9.134952524336056	0.0
72-73	0.0	2.077164155790635E-5	0.0	9.73553338252058	0.0
74-75	0.0	2.077164155790635E-5	0.0	10.31969425805358	0.0
76-77	0.0	2.077164155790635E-5	0.0	10.925114908721099	0.0
78-79	0.0	2.077164155790635E-5	0.0	11.568713836572048	0.0
80-81	0.0	2.077164155790635E-5	0.0	12.228016125440774	0.0
82-83	0.0	2.077164155790635E-5	0.0	12.874159579382567	0.0
84-85	0.0	2.077164155790635E-5	0.0	13.495127803756178	0.0
86-87	0.0	2.077164155790635E-5	0.0	14.130615405578764	0.0
88	0.0	2.077164155790635E-5	0.0	14.622425562745313	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7690	0.0	55.756004	1
GTATCAA	13905	0.0	40.4036	1
ATCAACG	17025	0.0	32.628387	3
TCAACGC	17075	0.0	32.615414	4
CAACGCA	17455	0.0	31.878439	5
TATCAAC	18015	0.0	31.01793	2
AACGCAG	18150	0.0	30.76323	6
GTGGTAT	3650	0.0	30.526613	1
TGGTATC	3700	0.0	28.451916	2
ACGCAGA	21075	0.0	26.40432	7
CGCAGAG	21255	0.0	25.82662	8
GTACATG	13030	0.0	23.777386	1
GCAGAGT	22950	0.0	23.304274	9
TACATGG	13035	0.0	22.786167	2
ACATGGG	13040	0.0	21.660183	3
GAGTACT	15465	0.0	21.297062	12-13
CAGAGTA	22130	0.0	20.79875	10-11
AGAGTAC	21215	0.0	20.043947	10-11
GTACTTT	16960	0.0	19.300142	14-15
AGTACTT	16230	0.0	18.656443	12-13
>>END_MODULE