FastQCFastQC Report
Wed 25 May 2016
SRR2049371_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049371_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8106049
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81550.044.9672851
GTATCAA132650.034.5559921
TCAACGC140850.031.038154
ATCAACG144250.030.3717523
CAACGCA148000.029.5706185
AACGCAG156050.027.9511936
TATCAAC167100.026.7530822
GTACATG221300.023.5388031
ACGCAGA184750.023.2782387
TACATGG224950.022.4015732
CGCAGAG190900.022.2082378
ACATGGG233600.020.9482443
GCAGAGT218450.018.3532569
GTGGTAT48850.017.804641
CATGGGG175550.017.6463264
AGAGTAC188050.016.94620710-11
CAGAGTA207300.016.77832410-11
GAGTACA170550.016.677451
GTATAGA35650.016.6164041
TGGTATC49250.015.5579042
Produced by FastQC (version 0.11.5)