##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049371_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8106049
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.984472336646373	31.0	31.0	34.0	28.0	34.0
2	31.08531776701572	31.0	31.0	34.0	28.0	34.0
3	31.265602268133343	31.0	31.0	34.0	28.0	34.0
4	34.85084940887972	37.0	35.0	37.0	32.0	37.0
5	34.8957377385703	37.0	35.0	37.0	32.0	37.0
6	34.850150424701354	37.0	35.0	37.0	32.0	37.0
7	34.82875245387734	37.0	35.0	37.0	32.0	37.0
8	34.816972485609206	37.0	35.0	37.0	32.0	37.0
9	36.43002083999246	39.0	37.0	39.0	32.0	39.0
10-11	36.39632729829292	39.0	37.0	39.0	32.0	39.0
12-13	36.34120173712249	39.0	37.0	39.0	32.0	39.0
14-15	37.56520507092913	40.0	38.0	41.0	32.0	41.0
16-17	37.52961837511715	40.0	38.0	41.0	32.0	41.0
18-19	37.548623564945146	40.0	38.0	41.0	32.0	41.0
20-21	37.63236750727759	40.0	38.0	41.0	32.0	41.0
22-23	37.58127375001064	40.0	38.0	41.0	32.0	41.0
24-25	37.58938935602289	40.0	38.0	41.0	32.0	41.0
26-27	37.58552656170719	40.0	38.0	41.0	32.0	41.0
28-29	37.525913364204925	40.0	38.0	41.0	32.0	41.0
30-31	37.46000369600529	40.0	38.0	41.0	32.0	41.0
32-33	37.41584315614179	40.0	38.0	41.0	32.0	41.0
34-35	37.315112331544015	40.0	37.5	41.0	31.0	41.0
36-37	37.2322617960982	40.0	37.0	41.0	31.0	41.0
38-39	37.13474640974907	40.0	37.0	41.0	31.0	41.0
40-41	37.0355262471273	40.0	37.0	41.0	31.0	41.0
42-43	36.89257997330142	40.0	37.0	41.0	30.0	41.0
44-45	36.76396787140072	40.0	37.0	41.0	30.0	41.0
46-47	36.652349930280465	40.0	36.5	41.0	30.0	41.0
48-49	36.53012861136171	40.0	36.0	41.0	30.0	41.0
50-51	36.39187957042944	40.0	36.0	41.0	30.0	41.0
52-53	36.25384499896312	39.0	36.0	41.0	30.0	41.0
54-55	36.11863325770668	39.0	35.0	41.0	29.0	41.0
56-57	35.995899913755764	39.0	35.0	41.0	29.0	41.0
58-59	35.792865180064915	39.0	35.0	41.0	28.5	41.0
60-61	35.578278887778744	39.0	35.0	40.0	28.0	41.0
62-63	35.37173264064897	38.0	35.0	40.0	28.0	41.0
64-65	35.12153158709009	38.0	34.5	40.0	28.0	41.0
66-67	34.85514034025701	37.5	34.0	40.0	28.0	41.0
68-69	34.563966736445835	37.0	34.0	40.0	27.5	41.0
70-71	34.27192822298508	36.5	34.0	39.5	27.0	41.0
72-73	33.919167216975865	36.0	34.0	39.0	26.0	41.0
74-75	33.55945535241645	35.5	34.0	39.0	26.0	40.0
76-77	33.21392838854046	35.0	33.0	38.0	26.0	40.0
78-79	32.885706896170994	35.0	33.0	37.0	26.0	39.0
80-81	32.51724274057559	35.0	33.0	37.0	25.5	39.0
82-83	32.19221201352225	35.0	33.0	36.0	25.0	39.0
84-85	31.88756396611962	35.0	33.0	36.0	24.5	37.0
86-87	31.556244231930993	35.0	32.5	35.5	23.5	37.0
88-89	31.296182825936533	35.0	32.0	35.0	22.5	36.5
90-91	31.081973104282987	35.0	32.0	35.0	21.0	36.0
92-93	30.832385851602922	35.0	32.0	35.0	19.5	36.0
94-95	30.622594743752472	35.0	32.0	35.0	17.5	36.0
96-97	30.363249099530485	35.0	32.0	35.0	6.5	35.5
98-99	30.146764780227706	35.0	32.0	35.0	2.0	35.0
100	30.01592131999202	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.401412274401693
1101	2	0.37165168536244053
1101	3	0.3841732968483562
1101	4	0.4087890057845627
1101	5	0.41222591894937466
1101	6	0.44821721906083667
1101	7	0.4856791991355678
1101	8	0.4953597647306367
1101	9	0.6328107685336875
1101	10-11	0.6402060903257265
1101	12-13	0.6556207147082347
1101	14-15	0.7164165765677453
1101	16-17	0.748260514372987
1101	18-19	0.7025079340726137
1101	20-21	0.6457431120952535
1101	22-23	0.6534652321544385
1101	24-25	0.6975203955573335
1101	26-27	0.6773974657304507
1101	28-29	0.7004872311207606
1101	30-31	0.7097834405218606
1101	32-33	0.6335315194325233
1101	34-35	0.6651977808976497
1101	36-37	0.6699281437286047
1101	38-39	0.6765573741121145
1101	40-41	0.7247502364068978
1101	42-43	0.6990011415236026
1101	44-45	0.6012105327728747
1101	46-47	0.5875057785336395
1101	48-49	0.5153141018929759
1101	50-51	0.6085669920562466
1101	52-53	0.6027231998225702
1101	54-55	0.5841723685210525
1101	56-57	0.5553728441584411
1101	58-59	0.6665979137837397
1101	60-61	0.6583196700688418
1101	62-63	0.6343955959807701
1101	64-65	0.5839009054407569
1101	66-67	0.6178065889500814
1101	68-69	0.5552782731613917
1101	70-71	0.6002945753570472
1101	72-73	0.7061793477168905
1101	74-75	0.6807876355446751
1101	76-77	0.6963861087250436
1101	78-79	0.6268571388310136
1101	80-81	0.6117379663025844
1101	82-83	0.6516429708628735
1101	84-85	0.6345455707459422
1101	86-87	0.6283601201597762
1101	88-89	0.6628422737261488
1101	90-91	0.6225820075447395
1101	92-93	0.6531432083204329
1101	94-95	0.6267906626034438
1101	96-97	0.6189616759684853
1101	98-99	0.6925885935600427
1101	100	0.699608146438532
1102	1	0.1752014593239224
1102	2	0.22603367195070234
1102	3	0.26655106343137547
1102	4	0.2299851787467091
1102	5	0.17394220088777956
1102	6	0.2713332511573583
1102	7	0.35287974590009696
1102	8	0.383453833653725
1102	9	0.430175446666091
1102	10-11	0.353076631581807
1102	12-13	0.43621878549572557
1102	14-15	0.5155512793233754
1102	16-17	0.48852292289900134
1102	18-19	0.48318624616467787
1102	20-21	0.4033446770596356
1102	22-23	0.4129075958909567
1102	24-25	0.47018865433829404
1102	26-27	0.4312450190219437
1102	28-29	0.46255532009711686
1102	30-31	0.47253788455304147
1102	32-33	0.46382778294665883
1102	34-35	0.47639854031027795
1102	36-37	0.45056912077988187
1102	38-39	0.4586003298106931
1102	40-41	0.47827884895266237
1102	42-43	0.4320412755154379
1102	44-45	0.3681263233451091
1102	46-47	0.3262166171517933
1102	48-49	0.379323114719341
1102	50-51	0.42808490079176664
1102	52-53	0.4213292837550071
1102	54-55	0.44455926249361255
1102	56-57	0.357531948086077
1102	58-59	0.4427260511277282
1102	60-61	0.3931892132128638
1102	62-63	0.42610887259748154
1102	64-65	0.35498175016370936
1102	66-67	0.42582567784326386
1102	68-69	0.44280495618077254
1102	70-71	0.39864068326927793
1102	72-73	0.3761503224298153
1102	74-75	0.36086347087501736
1102	76-77	0.38259083076809475
1102	78-79	0.336615084885878
1102	80-81	0.3238183237396939
1102	82-83	0.3296619695179359
1102	84-85	0.3646418234315121
1102	86-87	0.3714400805491671
1102	88-89	0.3343202054056924
1102	90-91	0.42806110133431474
1102	92-93	0.3519641295966984
1102	94-95	0.37120925448076036
1102	96-97	0.39413241825940304
1102	98-99	0.47524565889087
1102	100	0.45059238537023916
1103	1	0.17683200627954676
1103	2	0.21613171116638696
1103	3	0.27138766473856535
1103	4	0.25857651001605575
1103	5	0.23857928379111826
1103	6	0.3270281255983605
1103	7	0.3641422158003351
1103	8	0.34556425677033786
1103	9	0.4400189279182385
1103	10-11	0.4246443990396713
1103	12-13	0.4177401133251024
1103	14-15	0.4905013997224188
1103	16-17	0.5153985678938398
1103	18-19	0.4844117363607552
1103	20-21	0.4646415466846818
1103	22-23	0.4675266189387699
1103	24-25	0.5010649873276236
1103	26-27	0.47582620237247397
1103	28-29	0.45382552993544323
1103	30-31	0.5056974992761241
1103	32-33	0.5543833385022126
1103	34-35	0.521688357991728
1103	36-37	0.5074396058159962
1103	38-39	0.4896521013965227
1103	40-41	0.44928590089624976
1103	42-43	0.4472364939536959
1103	44-45	0.3794584526880769
1103	46-47	0.4218091524115053
1103	48-49	0.47643697780843297
1103	50-51	0.4280401811495267
1103	52-53	0.4711495451929224
1103	54-55	0.4357563729167282
1103	56-57	0.33691361303275613
1103	58-59	0.41219715535889634
1103	60-61	0.3487370288303424
1103	62-63	0.3557442355145781
1103	64-65	0.402445630452668
1103	66-67	0.5004945804920453
1103	68-69	0.4505844539447921
1103	70-71	0.42898468051730276
1103	72-73	0.40348606374387685
1103	74-75	0.40510581693624914
1103	76-77	0.4763068610398591
1103	78-79	0.3911962682364134
1103	80-81	0.3511454651225634
1103	82-83	0.42217658940097635
1103	84-85	0.4516980669815638
1103	86-87	0.45259935127500484
1103	88-89	0.4528969167919996
1103	90-91	0.4921890683105765
1103	92-93	0.4553482025241138
1103	94-95	0.4867699699950343
1103	96-97	0.4550792706845854
1103	98-99	0.4304245374598423
1103	100	0.3815211779398986
1104	1	0.1726288602368733
1104	2	0.17363125801450252
1104	3	0.19526065825386496
1104	4	0.2571949810582268
1104	5	0.26556229288929956
1104	6	0.310073969334276
1104	7	0.3552294929400688
1104	8	0.4036609253638801
1104	9	0.4031423190559238
1104	10-11	0.4546635474118901
1104	12-13	0.4049945781479636
1104	14-15	0.5638512819721768
1104	16-17	0.5243729877945071
1104	18-19	0.48379271011015135
1104	20-21	0.4637043835730239
1104	22-23	0.4802931237518777
1104	24-25	0.535625198012518
1104	26-27	0.44347236960777536
1104	28-29	0.492406435681751
1104	30-31	0.45513299840712307
1104	32-33	0.4461377097515893
1104	34-35	0.5463307532028452
1104	36-37	0.48617386300787047
1104	38-39	0.5587735831503764
1104	40-41	0.5262710394187664
1104	42-43	0.49202553725181986
1104	44-45	0.47275154076712766
1104	46-47	0.39165148325514565
1104	48-49	0.4128775783822718
1104	50-51	0.4823736548863664
1104	52-53	0.4371016947775317
1104	54-55	0.47281679611125327
1104	56-57	0.4444395006867481
1104	58-59	0.5050729065652604
1104	60-61	0.4393435364896092
1104	62-63	0.4122912068932081
1104	64-65	0.4535636950864159
1104	66-67	0.5110666962170782
1104	68-69	0.4769877142838794
1104	70-71	0.4485276238214695
1104	72-73	0.48632880714184523
1104	74-75	0.5766413560761947
1104	76-77	0.5824668008896765
1104	78-79	0.5212048084398617
1104	80-81	0.5943379292456541
1104	82-83	0.5085795831292188
1104	84-85	0.522303090968748
1104	86-87	0.5098432979535232
1104	88-89	0.49556449945738024
1104	90-91	0.49602845933676676
1104	92-93	0.4502654787168545
1104	94-95	0.5142550168484306
1104	96-97	0.5290135976800237
1104	98-99	0.4948463970220587
1104	100	0.5474303408528058
1105	1	0.17187638260381988
1105	2	0.17319655818554125
1105	3	0.23677563753736663
1105	4	0.2520733657272416
1105	5	0.26934592329236295
1105	6	0.28095302834427827
1105	7	0.2908533247377605
1105	8	0.3735331519923548
1105	9	0.44355232930382016
1105	10-11	0.4191595831189474
1105	12-13	0.37912907566082765
1105	14-15	0.4400281247197455
1105	16-17	0.4051096721895391
1105	18-19	0.37295270093085264
1105	20-21	0.409276967057302
1105	22-23	0.41236194490319633
1105	24-25	0.39953745727371626
1105	26-27	0.39775415326119656
1105	28-29	0.43949939886405076
1105	30-31	0.4301831619723515
1105	32-33	0.43160964091558895
1105	34-35	0.4443150373699396
1105	36-37	0.45245452274125597
1105	38-39	0.4557476049839764
1105	40-41	0.5305535242454695
1105	42-43	0.5217576998245264
1105	44-45	0.44912795012887585
1105	46-47	0.4136911400148122
1105	48-49	0.4325609340496328
1105	50-51	0.47311805715643374
1105	52-53	0.4485677136705064
1105	54-55	0.46218054954372434
1105	56-57	0.4429263673661339
1105	58-59	0.44772568304444604
1105	60-61	0.5020721959288323
1105	62-63	0.4154763900417464
1105	64-65	0.5004237748815825
1105	66-67	0.4715551539524938
1105	68-69	0.44867727025540916
1105	70-71	0.4702885849058802
1105	72-73	0.4353180644770447
1105	74-75	0.4948253244558174
1105	76-77	0.5767379529342236
1105	78-79	0.5068774849620894
1105	80-81	0.5118565697578958
1105	82-83	0.5132341383801204
1105	84-85	0.5684685399265561
1105	86-87	0.5163677774448558
1105	88-89	0.508872782133011
1105	90-91	0.5060463000559814
1105	92-93	0.5340242917265563
1105	94-95	0.5425162821288652
1105	96-97	0.507315956131567
1105	98-99	0.5079860461433547
1105	100	0.5333134590763926
1106	1	0.09484870304178727
1106	2	0.09154307593778555
1106	3	0.10697146692306703
1106	4	0.11716902199651003
1106	5	0.10678270038384596
1106	6	0.17678406172808536
1106	7	0.15613449914152255
1106	8	0.1552783438093499
1106	9	0.2164522023332509
1106	10-11	0.22080212228031115
1106	12-13	0.21890216751454972
1106	14-15	0.31395251610570085
1106	16-17	0.35738714162497587
1106	18-19	0.3327662270321383
1106	20-21	0.3036804361262426
1106	22-23	0.3251198390109238
1106	24-25	0.3355361577121059
1106	26-27	0.27852858211663545
1106	28-29	0.3533918991299032
1106	30-31	0.3183870620244704
1106	32-33	0.31914628855830784
1106	34-35	0.3558293898294025
1106	36-37	0.33101348265828534
1106	38-39	0.37045990206166834
1106	40-41	0.39883390626488335
1106	42-43	0.3777596427577947
1106	44-45	0.3393390618613168
1106	46-47	0.3308581808956177
1106	48-49	0.3452666737141996
1106	50-51	0.3853055571063635
1106	52-53	0.4076861142440862
1106	54-55	0.4022019451496277
1106	56-57	0.3235385846873129
1106	58-59	0.40677254150166675
1106	60-61	0.37837631070343036
1106	62-63	0.3882413825481166
1106	64-65	0.4046519273105673
1106	66-67	0.43393245281379933
1106	68-69	0.49388380959473466
1106	70-71	0.4332487223543353
1106	72-73	0.4936114167430006
1106	74-75	0.5150711358505049
1106	76-77	0.49935149459904693
1106	78-79	0.3991144015807322
1106	80-81	0.4373452886690643
1106	82-83	0.4375409903416667
1106	84-85	0.45368002873054536
1106	86-87	0.5376965078882066
1106	88-89	0.48875729008428337
1106	90-91	0.45627887068465967
1106	92-93	0.4328292366193658
1106	94-95	0.4732266275141228
1106	96-97	0.4600954087443796
1106	98-99	0.44697237099732945
1106	100	0.41274018427353454
1107	1	0.09306481519258725
1107	2	0.05748088241383442
1107	3	0.050946505818856735
1107	4	0.06918075171273586
1107	5	0.07438448306380963
1107	6	0.106826711699469
1107	7	0.09047731124202585
1107	8	0.13689763222578932
1107	9	0.17748795061503841
1107	10-11	0.16025882024674587
1107	12-13	0.15587764904114465
1107	14-15	0.13706283131259767
1107	16-17	0.19572701506217527
1107	18-19	0.14914552461290498
1107	20-21	0.15903158340112356
1107	22-23	0.218054549409203
1107	24-25	0.25871927711371256
1107	26-27	0.2718884869354241
1107	28-29	0.29789245495106087
1107	30-31	0.24146987840320833
1107	32-33	0.23478058512016275
1107	34-35	0.26249924374954503
1107	36-37	0.21079831772181024
1107	38-39	0.26792885069272643
1107	40-41	0.2851174264889451
1107	42-43	0.2172994314093799
1107	44-45	0.16222822726457053
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2216	2	-1.092862331794926
2216	3	-1.2131711692231875
2216	4	-1.3773659788676085
2216	5	-1.3200555307039323
2216	6	-2.7313950105558717
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2216	9	-3.0452086804849543
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2216	12-13	-1.8706542224487066
2216	14-15	-2.115303429877194
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2216	64-65	-3.3770028254110898
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2216	80-81	-3.6001722721305995
2216	82-83	-3.820716273792634
2216	84-85	-3.8489966496242545
2216	86-87	-3.5262048559679506
2216	88-89	-3.4827826979566083
2216	90-91	-3.6628922686040184
2216	92-93	-3.690072123623768
2216	94-95	-3.5302541442758724
2216	96-97	-3.813149128514624
2216	98-99	-3.6355844633678203
2216	100	-3.2546623348468877
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	109877.0
3	18881.0
4	12152.0
5	10159.0
6	9518.0
7	10128.0
8	11070.0
9	12749.0
10	14476.0
11	16220.0
12	18536.0
13	20241.0
14	23310.0
15	23822.0
16	24313.0
17	25272.0
18	24562.0
19	22881.0
20	23207.0
21	24207.0
22	27397.0
23	32066.0
24	39062.0
25	48460.0
26	59996.0
27	71350.0
28	87899.0
29	110218.0
30	135657.0
31	162540.0
32	209634.0
33	277576.0
34	383353.0
35	559386.0
36	925814.0
37	1635605.0
38	2139947.0
39	742777.0
40	1731.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.27191477910476	20.23246077975451	14.21258016511203	23.2830442760287
2	15.30169621046131	20.11818998811041	40.822516816847724	23.757596984580555
3	18.22568540559713	25.147625678519052	30.51778548955621	26.108903426327608
4	12.570925237573475	16.80708755001206	38.956878492266505	31.665108720147963
5	13.01873644518015	37.7420613742989	35.794264925791296	13.444937254729656
6	30.812839697305773	37.825078241920885	17.8426137133607	13.51946834741264
7	26.88126697140843	32.54991793990026	22.7635012868712	17.80531380182011
8	24.649672952393434	36.63552744456346	20.710279349590007	18.0045202534531
9	24.737678090103966	17.084752623836184	21.827392059871844	36.35017722618801
10-11	23.680255000007406	27.05959503309313	29.20384820257429	20.056301764325177
12-13	24.049594554275053	24.579362980758106	29.28657535125722	22.08446711370962
14-15	21.60156042939824	26.85685896383297	26.28950694659266	25.252073660176126
16-17	21.181396320634267	30.20674395502228	26.94027128776813	21.671588436575316
18-19	21.074615165125287	29.193635056776163	28.888372462696367	20.843377315402183
20-21	22.163460986654783	28.402797172763382	28.46672606211741	20.967015778464432
22-23	21.890948673906475	28.249699712532404	28.421797676748444	21.437553936812666
24-25	21.697020019260954	28.389303783826612	28.389649338204748	21.52402685870769
26-27	21.41928595859843	28.3858179019859	28.458588095344318	21.73630804407135
28-29	21.49811906244624	28.37334012598493	28.58293242619841	21.545608385370425
30-31	21.72907387932139	28.699752599989424	28.19761567183082	21.373557848858358
32-33	21.428933292697593	28.495195568343046	28.52553661915539	21.550334519803968
34-35	21.631261340879295	28.211066266956784	28.544571267711454	21.613101124452463
36-37	21.86841070572198	28.49391332163247	28.096806335743356	21.540869636902197
38-39	21.706685424977554	28.580366095587166	28.24584494158082	21.467103537854456
40-41	21.570990901489886	28.33669732413095	28.41988282081581	21.67242895356335
42-43	21.52584291946294	28.32245678397346	28.374733824893017	21.77696647167059
44-45	21.638266448765915	28.421133822303936	28.284037762195908	21.656561966734245
46-47	21.603407853177387	28.528404823497382	28.202451979880454	21.665735343444773
48-49	21.585456039821512	28.35552684246851	28.264124200967338	21.79489291674264
50-51	21.598492864077194	28.291298654587106	28.229019647721604	21.8811888336141
52-53	21.563907829553948	28.287638068300424	28.28640395892415	21.862050143221477
54-55	21.687163831198834	28.531419935550367	28.109586092780614	21.671830140470185
56-57	21.535753688040064	28.38542579369683	28.177397380300672	21.90142313796244
58-59	21.45798196515168	28.03210185476739	28.438511933421413	22.071404246659522
60-61	21.63967523901081	28.375960578488225	28.11453884211033	21.869825340390637
62-63	21.521587042899625	28.3992957818612	28.121750114862444	21.957367060376733
64-65	21.555376221311757	28.124404380195372	28.261827694290137	22.058391704202734
66-67	21.445996372353772	28.17214190814981	28.12696909886138	22.254892620635033
68-69	21.720129471500357	28.291584963342796	28.081021313607422	21.907264251549424
70-71	21.640428157770305	28.120630356067768	28.144010197535284	22.094931288626643
72-73	21.48701871252728	28.210632703946924	28.249054871445857	22.053293712079935
74-75	21.54642621703009	28.147738213736652	28.185191748523515	22.12064382070974
76-77	21.563435188830244	28.158653685962896	28.053647213299254	22.224263911907602
78-79	21.602154534421977	28.193786172072006	28.14645966905755	22.057599624448464
80-81	21.523527779889406	28.086413735093675	28.198997886285937	22.19106059873098
82-83	21.43798414480244	28.09578868720467	28.2987220363503	22.16750513164259
84-85	21.503279225647375	27.966725840199373	28.24402078909973	22.28597414505352
86-87	21.611989694516737	28.05887729862763	28.165951501735286	22.163181505120345
88-89	21.48360519483212	27.953111650732854	28.21916063602966	22.344122518405367
90-91	21.48997341530302	27.888651560292914	28.250134083526447	22.37124094087762
92-93	21.607134527201147	27.956179076716666	28.10908595487911	22.327600441203074
94-95	21.471673464189507	27.77735750469845	28.236898350120327	22.51407068099171
96-97	21.546841130617395	27.732832604392105	28.25438755674929	22.465938708241215
98-99	21.58410959519243	27.779020334073973	28.158015082316922	22.478854988416675
100	21.614599171556943	27.697241899228587	28.167939769424045	22.52021915979042
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1684.0
1	1395.0
2	1351.5
3	1730.5
4	1713.5
5	1437.5
6	1187.0
7	1043.0
8	1063.5
9	1065.5
10	961.5
11	905.5
12	960.5
13	1068.0
14	1200.5
15	1369.0
16	1658.0
17	2126.0
18	2866.0
19	4137.0
20	5950.5
21	8633.5
22	12616.5
23	18070.5
24	26080.0
25	36937.0
26	51778.0
27	69790.0
28	91006.5
29	115930.0
30	140704.0
31	163611.0
32	184392.5
33	204662.5
34	224129.5
35	240982.0
36	257746.0
37	273557.5
38	285565.5
39	293673.0
40	300050.0
41	304115.0
42	304817.5
43	303806.0
44	299228.0
45	292014.5
46	285695.5
47	277442.0
48	266368.5
49	256883.0
50	249600.0
51	240816.5
52	225924.0
53	211140.0
54	200843.5
55	190583.0
56	174366.0
57	154914.5
58	139186.5
59	125723.0
60	110194.0
61	93351.5
62	78434.0
63	64801.5
64	52059.5
65	40955.5
66	32422.5
67	24892.0
68	18669.0
69	14072.0
70	10189.0
71	7437.0
72	5574.0
73	3955.5
74	2790.0
75	1928.5
76	1310.0
77	920.5
78	641.5
79	426.5
80	295.5
81	233.5
82	175.0
83	134.5
84	107.5
85	88.5
86	73.5
87	58.0
88	58.0
89	51.5
90	38.5
91	31.0
92	30.5
93	27.0
94	22.5
95	19.0
96	17.0
97	19.5
98	30.0
99	33.0
100	40.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016900958777821353
2	0.03902024278412331
3	0.04061164693181598
4	0.0432146413129257
5	0.04644679547335576
6	0.04459632553417824
7	0.04257314506734415
8	0.04201800408559089
9	0.042055013484374446
10-11	0.042696509729955985
12-13	0.04375127759528718
14-15	0.04203650878498267
16-17	0.0382862230415829
18-19	0.041364171373748176
20-21	0.040660992796860716
22-23	0.04190080765610966
24-25	0.0385822982318513
26-27	0.038779681692030235
28-29	0.039168280379257514
30-31	0.03922379447743284
32-33	0.03905108394977627
34-35	0.03913127098047397
36-37	0.03630622020666295
38-39	0.03668865066075964
40-41	0.03792229728687799
42-43	0.03781743732365792
44-45	0.036657809495106684
46-47	0.03556603223099194
48-49	0.035670892194211996
50-51	0.03697855761789745
52-53	0.037496689200867156
54-55	0.03672566005954319
56-57	0.03489986305288804
58-59	0.035818929789346206
60-61	0.03526995704072354
62-63	0.035504349899686025
64-65	0.03232770983743128
66-67	0.03359219762920259
68-69	0.03452360083192194
70-71	0.03595463091821922
72-73	0.03274098145718093
74-75	0.03300004724866578
76-77	0.03378958108938152
78-79	0.03413500214469466
80-81	0.0342583668073065
82-83	0.032685467359005606
84-85	0.034097992745911104
86-87	0.03441874086870188
88-89	0.03506023711428342
90-91	0.035664723961081404
92-93	0.033937618684515725
94-95	0.03518360177689526
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8106049.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.27516783401896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.30319783663472	43.30031324534204
2	14.744569456412723	17.184845194256024
3	4.958031557462342	8.667903634124412
4	2.091150842304114	4.874486652060894
5	1.09770908738619	3.198459065017901
6	0.6573541814189852	2.29844551491513
7	0.4207763860749028	1.7164570163374848
8	0.2941912889871138	1.3715237392824344
9	0.2137316043292063	1.1209720602346767
>10	1.1350790692198045	11.74088292803071
>50	0.06273972159301448	2.488619867148851
>100	0.021172143877717143	1.9036455526964295
>500	2.332640395140078E-4	0.07489788139043863
>1k	6.356025958610156E-5	0.05854764916249189
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.233646626118347E-5	0.0
10-11	0.0	0.0	0.0	3.700939878355041E-5	0.0
12-13	0.0	0.0	0.0	6.168233130591735E-5	0.0
14-15	0.0	0.0	0.0	8.018703069769255E-5	0.0
16-17	0.0	0.0	0.0	8.635526382828429E-5	0.0
18-19	0.0	1.233646626118347E-5	0.0	9.252349695887602E-5	0.0
20-21	0.0	1.233646626118347E-5	0.0	1.8504699391775205E-4	0.0
22-23	0.0	1.233646626118347E-5	0.0	3.9476692035787103E-4	6.168233130591735E-6
24-25	0.0	1.233646626118347E-5	0.0	8.018703069769255E-4	1.233646626118347E-5
26-27	0.0	1.233646626118347E-5	0.0	0.0016777594115209519	1.233646626118347E-5
28-29	0.0	1.233646626118347E-5	0.0	0.005181315829697057	1.233646626118347E-5
30-31	0.0	1.233646626118347E-5	0.0	0.016796098814601295	1.233646626118347E-5
32-33	6.168233130591735E-6	1.233646626118347E-5	0.0	0.04326398717797043	1.233646626118347E-5
34-35	1.233646626118347E-5	2.467293252236694E-5	0.0	0.08382012001161109	1.233646626118347E-5
36-37	1.233646626118347E-5	2.467293252236694E-5	0.0	0.1500669438341663	1.233646626118347E-5
38-39	1.233646626118347E-5	2.467293252236694E-5	0.0	0.23570052438617134	1.233646626118347E-5
40-41	1.233646626118347E-5	2.467293252236694E-5	0.0	0.3241221463132039	1.233646626118347E-5
42-43	1.233646626118347E-5	2.467293252236694E-5	0.0	0.41387610659644425	1.233646626118347E-5
44-45	1.233646626118347E-5	3.0841165652958674E-5	0.0	0.5256444909227664	1.233646626118347E-5
46-47	2.467293252236694E-5	3.700939878355041E-5	0.0	0.6583540267274477	1.233646626118347E-5
48-49	2.467293252236694E-5	3.700939878355041E-5	0.0	0.7949310447050097	1.233646626118347E-5
50-51	2.467293252236694E-5	3.700939878355041E-5	0.0	0.9291394611604247	1.233646626118347E-5
52-53	2.467293252236694E-5	3.700939878355041E-5	0.0	1.062422642646251	1.233646626118347E-5
54-55	2.467293252236694E-5	3.700939878355041E-5	0.0	1.208066963325783	1.233646626118347E-5
56-57	2.467293252236694E-5	4.3177631914142144E-5	0.0	1.374788136612547	1.233646626118347E-5
58-59	2.467293252236694E-5	4.934586504473388E-5	0.0	1.557417183143107	1.233646626118347E-5
60-61	2.467293252236694E-5	4.934586504473388E-5	0.0	1.7395466027900892	1.233646626118347E-5
62-63	3.700939878355041E-5	4.934586504473388E-5	0.0	1.9193752714793606	1.233646626118347E-5
64-65	3.700939878355041E-5	4.934586504473388E-5	0.0	2.1084994674964337	1.233646626118347E-5
66-67	3.700939878355041E-5	4.934586504473388E-5	0.0	2.324745384588719	1.233646626118347E-5
68-69	3.700939878355041E-5	4.934586504473388E-5	0.0	2.560452077208021	1.233646626118347E-5
70-71	4.3177631914142144E-5	4.934586504473388E-5	0.0	2.8057997182104377	1.233646626118347E-5
72-73	4.934586504473388E-5	4.934586504473388E-5	0.0	3.0560264316191526	1.233646626118347E-5
74-75	6.168233130591735E-5	4.934586504473388E-5	0.0	3.308294830194093	1.233646626118347E-5
76-77	6.168233130591735E-5	6.168233130591735E-5	0.0	3.578734843571757	1.233646626118347E-5
78-79	6.168233130591735E-5	6.168233130591735E-5	0.0	3.875969661668712	1.233646626118347E-5
80-81	6.168233130591735E-5	6.785056443650908E-5	0.0	4.188921137782414	1.233646626118347E-5
82-83	6.168233130591735E-5	8.018703069769255E-5	0.0	4.507843463566529	1.233646626118347E-5
84-85	6.168233130591735E-5	9.869173008946776E-5	0.0	4.8260132649087115	1.233646626118347E-5
86-87	6.168233130591735E-5	9.869173008946776E-5	0.0	5.159511125580416	1.233646626118347E-5
88	6.168233130591735E-5	9.869173008946776E-5	0.0	5.421728884195001	1.233646626118347E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8155	0.0	44.967285	1
GTATCAA	13265	0.0	34.555992	1
TCAACGC	14085	0.0	31.03815	4
ATCAACG	14425	0.0	30.371752	3
CAACGCA	14800	0.0	29.570618	5
AACGCAG	15605	0.0	27.951193	6
TATCAAC	16710	0.0	26.753082	2
GTACATG	22130	0.0	23.538803	1
ACGCAGA	18475	0.0	23.278238	7
TACATGG	22495	0.0	22.401573	2
CGCAGAG	19090	0.0	22.208237	8
ACATGGG	23360	0.0	20.948244	3
GCAGAGT	21845	0.0	18.353256	9
GTGGTAT	4885	0.0	17.80464	1
CATGGGG	17555	0.0	17.646326	4
AGAGTAC	18805	0.0	16.946207	10-11
CAGAGTA	20730	0.0	16.778324	10-11
GAGTACA	17055	0.0	16.67745	1
GTATAGA	3565	0.0	16.616404	1
TGGTATC	4925	0.0	15.557904	2
>>END_MODULE