FastQCFastQC Report
Wed 25 May 2016
SRR2049371_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049371_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8106049
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161880.19970271583603802No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158100.19503953158931064No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91280.11260726403208271No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123800.056.7343831
GTATCAA222900.039.5675471
TCAACGC271850.031.9989224
ATCAACG272850.031.812753
CAACGCA279900.031.1289965
TATCAAC288700.030.2289732
AACGCAG291100.030.011596
GTGGTAT54350.028.4585481
ACGCAGA336950.025.7464547
CGCAGAG344400.025.0258968
TGGTATC54900.024.8246942
GTACATG215050.022.9106921
GCAGAGT377650.022.4983279
TACATGG220150.021.4752162
GAGTACT259450.020.93243212-13
CAGAGTA368300.020.22731810-11
AGAGTAC348250.020.18955610-11
ACATGGG224100.020.0691223
AGTACTT273250.019.0837112-13
GTACTTT287800.018.79490514-15
Produced by FastQC (version 0.11.5)