##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049371_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8106049
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.873651022834924	31.0	31.0	34.0	30.0	34.0
2	31.93732248596079	33.0	31.0	34.0	30.0	34.0
3	31.920751280926133	33.0	31.0	34.0	30.0	34.0
4	35.66868865460843	37.0	35.0	37.0	33.0	37.0
5	35.764875341858904	37.0	35.0	37.0	35.0	37.0
6	35.83106171699678	37.0	35.0	37.0	35.0	37.0
7	35.794508644100226	37.0	35.0	37.0	35.0	37.0
8	35.80459358190408	37.0	35.0	37.0	35.0	37.0
9	37.544974623272076	39.0	37.0	39.0	35.0	39.0
10-11	37.51116407019005	39.0	37.0	39.0	35.0	39.0
12-13	37.466847103934356	39.0	37.0	39.0	34.5	39.0
14-15	38.772269326277204	40.0	38.0	41.0	35.0	41.0
16-17	38.80530632124232	40.0	38.0	41.0	35.0	41.0
18-19	38.86805976623137	40.0	38.0	41.0	35.0	41.0
20-21	38.89703565818563	40.0	38.0	41.0	35.0	41.0
22-23	38.86735708111313	40.0	38.0	41.0	35.0	41.0
24-25	38.85043656903628	40.0	38.0	41.0	35.0	41.0
26-27	38.743876147306786	40.0	38.0	41.0	35.0	41.0
28-29	38.66680777527992	40.0	38.0	41.0	34.0	41.0
30-31	38.573540327723165	40.0	38.0	41.0	34.0	41.0
32-33	38.4513680462578	40.0	38.0	41.0	34.0	41.0
34-35	38.3558567188528	40.0	38.0	41.0	34.0	41.0
36-37	38.25245659136775	40.0	38.0	41.0	34.0	41.0
38-39	38.1676612736982	40.0	38.0	41.0	34.0	41.0
40-41	38.064256088261985	40.0	38.0	41.0	33.0	41.0
42-43	37.94070193752838	40.0	38.0	41.0	33.0	41.0
44-45	37.78177074922691	40.0	37.0	41.0	33.0	41.0
46-47	37.57418934921316	40.0	37.0	41.0	33.0	41.0
48-49	37.38523021511466	40.0	37.0	41.0	32.5	41.0
50-51	37.24780099404778	40.0	37.0	41.0	32.0	41.0
52-53	37.11617071399395	40.0	37.0	41.0	32.0	41.0
54-55	36.980282811021745	40.0	36.0	41.0	31.0	41.0
56-57	36.84108774817423	39.5	36.0	41.0	31.0	41.0
58-59	36.65223174693368	39.0	35.5	41.0	31.0	41.0
60-61	36.477928581482786	39.0	35.0	41.0	31.0	41.0
62-63	36.27438971809818	39.0	35.0	41.0	31.0	41.0
64-65	36.04768482154499	38.5	35.0	40.5	30.5	41.0
66-67	35.778481292180686	38.0	35.0	40.0	30.0	41.0
68-69	35.48672152117511	37.0	35.0	40.0	30.0	41.0
70-71	35.15244504443534	37.0	34.5	39.5	29.0	41.0
72-73	34.85109219053574	36.0	34.0	39.0	29.0	41.0
74-75	34.53871590216146	36.0	34.0	39.0	29.0	41.0
76-77	34.20770865066323	35.5	34.0	38.5	29.0	40.0
78-79	33.86620775423391	35.0	34.0	37.0	29.0	39.0
80-81	33.5442814989152	35.0	34.0	37.0	29.0	39.0
82-83	33.24050243219601	35.0	34.0	36.5	29.0	39.0
84-85	32.96681971697926	35.0	34.0	36.0	28.0	37.0
86-87	32.730868207187	35.0	33.5	36.0	28.0	37.0
88-89	32.503196193361276	35.0	33.0	35.0	27.5	37.0
90-91	32.309936567124126	35.0	33.0	35.0	27.0	36.0
92-93	32.130302321143134	35.0	33.0	35.0	27.0	36.0
94-95	31.974967459486123	35.0	33.0	35.0	27.0	36.0
96-97	31.774807677575104	35.0	33.0	35.0	26.0	36.0
98-99	31.628753231074718	35.0	33.0	35.0	26.0	35.0
100	31.51669352109764	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1830626913224691
1101	2	0.1891756746644333
1101	3	0.3818783597576463
1101	4	0.21309801760532565
1101	5	0.2220421534822279
1101	6	0.17346046247489966
1101	7	0.08727136527974722
1101	8	0.16237982891501446
1101	9	0.22568544071389596
1101	10-11	0.26144070223219984
1101	12-13	0.2242660299637862
1101	14-15	0.3389734036829637
1101	16-17	0.24918045640343678
1101	18-19	0.3115635258851839
1101	20-21	0.30901380387575017
1101	22-23	0.2882621620507422
1101	24-25	0.3329828206032346
1101	26-27	0.39851909611093106
1101	28-29	0.3742090565239522
1101	30-31	0.3381704000677601
1101	32-33	0.3511265025328356
1101	34-35	0.3930666219654171
1101	36-37	0.4051140830418376
1101	38-39	0.3840922286134898
1101	40-41	0.38524434284331477
1101	42-43	0.41755994837554056
1101	44-45	0.4338419385677099
1101	46-47	0.49720750565928284
1101	48-49	0.5030789161645615
1101	50-51	0.46084480963498464
1101	52-53	0.48004405960239893
1101	54-55	0.4755900313343915
1101	56-57	0.4134542193469173
1101	58-59	0.4263450976349432
1101	60-61	0.40693347756906917
1101	62-63	0.3979298205073789
1101	64-65	0.35364900471454064
1101	66-67	0.40694353220869317
1101	68-69	0.45684644961941956
1101	70-71	0.40775821539401846
1101	72-73	0.33323260452772985
1101	74-75	0.38560988196836377
1101	76-77	0.32990652544268784
1101	78-79	0.35994437437211957
1101	80-81	0.39034630106890944
1101	82-83	0.3440727885742021
1101	84-85	0.4047013561094701
1101	86-87	0.32972309176662407
1101	88-89	0.37860270248957306
1101	90-91	0.3963528594398227
1101	92-93	0.3926617291619152
1101	94-95	0.4187854806769238
1101	96-97	0.45454125746924134
1101	98-99	0.4829780259689862
1101	100	0.5347815255503363
1102	1	0.16296168740377937
1102	2	0.11330211474710694
1102	3	0.31751917594965917
1102	4	0.13846723275339912
1102	5	0.12903195108205523
1102	6	0.14385802023906535
1102	7	0.1097961336816553
1102	8	0.15870891085593541
1102	9	0.09624915431850667
1102	10-11	0.19124059331871734
1102	12-13	0.14138201576422915
1102	14-15	0.2340027585353397
1102	16-17	0.1942136326439723
1102	18-19	0.2317431115117401
1102	20-21	0.16937006004528854
1102	22-23	0.19911589704571497
1102	24-25	0.24589030456046146
1102	26-27	0.29454573911166904
1102	28-29	0.22800036008028002
1102	30-31	0.20204326318987142
1102	32-33	0.26476133711459937
1102	34-35	0.27224835839791695
1102	36-37	0.300854376951321
1102	38-39	0.3038923786441714
1102	40-41	0.2701118222807892
1102	42-43	0.24943982809989507
1102	44-45	0.25456360026735325
1102	46-47	0.20253224126158642
1102	48-49	0.19679288565309605
1102	50-51	0.2547253037632231
1102	52-53	0.3624669805553822
1102	54-55	0.2442001053610383
1102	56-57	0.30153159029462984
1102	58-59	0.337264216032068
1102	60-61	0.31289787196142527
1102	62-63	0.3161696828432454
1102	64-65	0.1961858454073777
1102	66-67	0.19110972414438976
1102	68-69	0.20518324731183668
1102	70-71	0.20689346350176407
1102	72-73	0.21119811159533697
1102	74-75	0.19016961803363586
1102	76-77	0.17547295403765162
1102	78-79	0.254398225813496
1102	80-81	0.25178484969470105
1102	82-83	0.2777604124554429
1102	84-85	0.22430443785892606
1102	86-87	0.25095681163297456
1102	88-89	0.2070285848222042
1102	90-91	0.2258278069840074
1102	92-93	0.2575277020151532
1102	94-95	0.22084347867755838
1102	96-97	0.2845395625619709
1102	98-99	0.26211169294795766
1102	100	0.27632695926612527
1103	1	0.16381480057887643
1103	2	0.1510867724698457
1103	3	0.2744111608137807
1103	4	0.17294951689513027
1103	5	0.17907323075181836
1103	6	0.1430960663346994
1103	7	0.14044628848041185
1103	8	0.10823763462614266
1103	9	0.20352813608665343
1103	10-11	0.2110393549286229
1103	12-13	0.21509460606350927
1103	14-15	0.2824943306027663
1103	16-17	0.24309564158789954
1103	18-19	0.2686256364515387
1103	20-21	0.23536163211270633
1103	22-23	0.21092618256343343
1103	24-25	0.20809790689964558
1103	26-27	0.2498054570339292
1103	28-29	0.3370148079646995
1103	30-31	0.2706380344316912
1103	32-33	0.2917935008572883
1103	34-35	0.3238703054223393
1103	36-37	0.3118115492939353
1103	38-39	0.3067699156238817
1103	40-41	0.33765138196431366
1103	42-43	0.2992351497373278
1103	44-45	0.32820653112391085
1103	46-47	0.30626031143702903
1103	48-49	0.29532143742468264
1103	50-51	0.2934275741450634
1103	52-53	0.27862023132936997
1103	54-55	0.23654444660630958
1103	56-57	0.20190654729497481
1103	58-59	0.304582757483729
1103	60-61	0.26422398135255776
1103	62-63	0.2700465318284486
1103	64-65	0.3088286042653081
1103	66-67	0.29389610185337034
1103	68-69	0.2955349844979409
1103	70-71	0.3396600613689813
1103	72-73	0.3140636086713613
1103	74-75	0.29967340200336423
1103	76-77	0.2749879179179473
1103	78-79	0.2673849819194487
1103	80-81	0.30636947301771045
1103	82-83	0.26415704128240947
1103	84-85	0.30718799479046766
1103	86-87	0.3219372037898367
1103	88-89	0.3900119009556704
1103	90-91	0.3436210646379507
1103	92-93	0.35129877184659364
1103	94-95	0.2797573073669284
1103	96-97	0.32774045695481036
1103	98-99	0.40594038231843754
1103	100	0.43266510856093277
1104	1	0.11316661593043165
1104	2	0.17507716132177364
1104	3	0.286955114815445
1104	4	0.17543895244013896
1104	5	0.16181203919867215
1104	6	0.14468837367676457
1104	7	0.17232052467927872
1104	8	0.13981292907965326
1104	9	0.19712034336308903
1104	10-11	0.18471272356884327
1104	12-13	0.17934767412250352
1104	14-15	0.23052912730073416
1104	16-17	0.21087301512130097
1104	18-19	0.22511933326895672
1104	20-21	0.24866055793066266
1104	22-23	0.24050711098897892
1104	24-25	0.25089399080173536
1104	26-27	0.24791284459556095
1104	28-29	0.31123092012407483
1104	30-31	0.2840178524057322
1104	32-33	0.31795624877725004
1104	34-35	0.2975402115839785
1104	36-37	0.28793193811642936
1104	38-39	0.2993254568101449
1104	40-41	0.2753828161973857
1104	42-43	0.3593444272928963
1104	44-45	0.3246581181423096
1104	46-47	0.34825366295876137
1104	48-49	0.3050779446771017
1104	50-51	0.279184971427604
1104	52-53	0.2537473865275359
1104	54-55	0.2498933835994137
1104	56-57	0.28482037970650964
1104	58-59	0.3600135339670629
1104	60-61	0.3282702485496003
1104	62-63	0.38038678700274176
1104	64-65	0.301169709824336
1104	66-67	0.3578567376267614
1104	68-69	0.3083650474336821
1104	70-71	0.38374689236834314
1104	72-73	0.3477147239616798
1104	74-75	0.353024159394586
1104	76-77	0.3320089026156907
1104	78-79	0.34664336747994184
1104	80-81	0.32007251336822407
1104	82-83	0.36605215968965865
1104	84-85	0.3497624367817451
1104	86-87	0.32256109915096687
1104	88-89	0.3658967763125034
1104	90-91	0.3150059296939034
1104	92-93	0.44724152166745057
1104	94-95	0.44060382884840976
1104	96-97	0.46420537278455853
1104	98-99	0.4495080146530732
1104	100	0.4638601795841595
1105	1	0.20830077149710036
1105	2	0.16576099767929975
1105	3	0.28883176093359353
1105	4	0.20811141891405072
1105	5	0.1822696116313196
1105	6	0.18088920483211268
1105	7	0.1789220297118419
1105	8	0.18371565183211658
1105	9	0.2294894419719853
1105	10-11	0.2505651099748434
1105	12-13	0.21434515114093244
1105	14-15	0.29911288039256334
1105	16-17	0.2684230474925897
1105	18-19	0.22137073392469375
1105	20-21	0.2768510640609989
1105	22-23	0.2790443950638348
1105	24-25	0.2559053093672006
1105	26-27	0.28574711964049015
1105	28-29	0.3424672167067939
1105	30-31	0.29844877837654593
1105	32-33	0.3353185069438922
1105	34-35	0.3415797459419778
1105	36-37	0.35244418638521324
1105	38-39	0.381440611988765
1105	40-41	0.34310955748600946
1105	42-43	0.3464373799852041
1105	44-45	0.4132920385260803
1105	46-47	0.38257155399958265
1105	48-49	0.3599642984288991
1105	50-51	0.3508244942687
1105	52-53	0.34739635908645283
1105	54-55	0.3059756380713736
1105	56-57	0.3301321087868416
1105	58-59	0.3969229090044166
1105	60-61	0.3434639696146462
1105	62-63	0.3505456734203207
1105	64-65	0.3472337785114874
1105	66-67	0.3636999334177844
1105	68-69	0.3424927245181024
1105	70-71	0.32970904800357914
1105	72-73	0.397128337439689
1105	74-75	0.3586982592433685
1105	76-77	0.33259891646718387
1105	78-79	0.34187016487622657
1105	80-81	0.40687902849367674
1105	82-83	0.37309697048701906
1105	84-85	0.36940740735276023
1105	86-87	0.3352469594148033
1105	88-89	0.33844709840087006
1105	90-91	0.3409829504787538
1105	92-93	0.4078438855544704
1105	94-95	0.39959832510744064
1105	96-97	0.41424249770136257
1105	98-99	0.44627882874037894
1105	100	0.49035822180621835
1106	1	0.12044661198397932
1106	2	0.08990625899281923
1106	3	0.18013632007579972
1106	4	0.11209558308394918
1106	5	0.0636001876422938
1106	6	0.11502587414842935
1106	7	0.12258589777054851
1106	8	0.07900666107373411
1106	9	0.15620439783138096
1106	10-11	0.12801325769630978
1106	12-13	0.12232425085536391
1106	14-15	0.1753077859529597
1106	16-17	0.1393924877662016
1106	18-19	0.18330741052607635
1106	20-21	0.17865425519723743
1106	22-23	0.17796081240128814
1106	24-25	0.16099540543338975
1106	26-27	0.19460390546230855
1106	28-29	0.19777367359776576
1106	30-31	0.1804881460884431
1106	32-33	0.13200543933704978
1106	34-35	0.1299351996490472
1106	36-37	0.1658869620655139
1106	38-39	0.2073218017638041
1106	40-41	0.13723865225738763
1106	42-43	0.20788650467449088
1106	44-45	0.15069113917304122
1106	46-47	0.2119748777944963
1106	48-49	0.16095408587858628
1106	50-51	0.1322142854677324
1106	52-53	0.16050252096433582
1106	54-55	0.15667281586173232
1106	56-57	0.1387666814304538
1106	58-59	0.1741356180034046
1106	60-61	0.11074862103470196
1106	62-63	0.09820541970243113
1106	64-65	0.05994492055386047
1106	66-67	0.06869090933717104
1106	68-69	0.12361783792663772
1106	70-71	0.18158285860587853
1106	72-73	0.22680625317616432
1106	74-75	0.18392536206752652
1106	76-77	0.16822060944686967
1106	78-79	0.2083232586975612
1106	80-81	0.16487129633710396
1106	82-83	0.1416551663801755
1106	84-85	0.1526461843868816
1106	86-87	0.18537326903744145
1106	88-89	0.1619889958302494
1106	90-91	0.14950860098459628
1106	92-93	0.19597254220715854
1106	94-95	0.18979516046236
1106	96-97	0.24592718448922923
1106	98-99	0.2845171833048106
1106	100	0.26079586034062174
1107	1	0.12446502558115924
1107	2	0.08494593016801133
1107	3	0.19900766608255438
1107	4	0.14380663167086283
1107	5	0.07391221897868405
1107	6	0.10658532168596224
1107	7	0.09076220432043414
1107	8	0.11417825648892688
1107	9	0.1486475873759403
1107	10-11	0.1653763528856942
1107	12-13	0.1387293231082154
1107	14-15	0.20654482799469776
1107	16-17	0.10332201283815579
1107	18-19	0.14771797514139706
1107	20-21	0.12122527581581011
1107	22-23	0.10291999763332171
1107	24-25	0.13666003350467548
1107	26-27	0.22167753920012245
1107	28-29	0.21326056942717742
1107	30-31	0.14819730375346296
1107	32-33	0.1750885732722196
1107	34-35	0.09081199437862608
1107	36-37	0.12404117798752168
1107	38-39	0.11707438829014194
1107	40-41	0.09030858519648177
1107	42-43	0.1316856738066008
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2216	74-75	-0.617615848379657
2216	76-77	-0.5439907777193085
2216	78-79	-0.6877945626010984
2216	80-81	-0.8502697561005093
2216	82-83	-0.7247115892270841
2216	84-85	-0.7967350552294405
2216	86-87	-0.6336751478862013
2216	88-89	-0.9296107094674646
2216	90-91	-0.7541121152491428
2216	92-93	-0.8605224245500125
2216	94-95	-0.7982111822004043
2216	96-97	-0.8943906050148165
2216	98-99	-0.9950965874934035
2216	100	-0.9663931652209143
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	471.0
3	31.0
4	55.0
5	112.0
6	297.0
7	863.0
8	2153.0
9	3764.0
10	5610.0
11	8117.0
12	11325.0
13	15968.0
14	19311.0
15	24231.0
16	30159.0
17	41682.0
18	41272.0
19	23151.0
20	18646.0
21	18774.0
22	20212.0
23	22242.0
24	26736.0
25	33065.0
26	42372.0
27	54907.0
28	72350.0
29	95495.0
30	124074.0
31	160921.0
32	208619.0
33	272853.0
34	372485.0
35	537896.0
36	877535.0
37	1620563.0
38	2339337.0
39	955036.0
40	3359.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.28395757707845	20.62909463656448	14.629028981690434	23.45791880466663
2	15.296897415744711	20.689462893698273	39.99176417512403	24.02187551543298
3	17.87314633800018	25.308457918278066	30.941670843588536	25.87672490013322
4	12.665160793679775	17.30465183986262	38.27311486249759	31.75707250396001
5	12.632751171378311	37.830810053085045	35.7884217082823	13.748017067254342
6	30.616333555348607	38.26194487598089	17.315241987804416	13.80647958086609
7	26.438663274796394	33.01233436906192	22.516185135323017	18.032817220818675
8	24.710053279236593	36.600336147225775	20.31319624592831	18.37641432760932
9	24.55930133163518	16.699084843923345	21.73755673078216	37.00405709365931
10-11	23.64202338278488	27.086161211213994	28.865819834052324	20.4059955719488
12-13	24.28522897782654	24.560253530835276	29.275091508747288	21.87942598259089
14-15	21.597465790628444	26.628918266985885	26.745374652157484	25.028241290228188
16-17	20.727565348270492	30.03956860115012	27.45385744977963	21.77900860079976
18-19	20.566772011903215	28.417936439048084	30.271577536076162	20.74371401297254
20-21	21.599121050530158	27.637463691428632	30.069594396919186	20.693820861122024
22-23	21.303973119333474	27.483919724640206	30.1008234714594	21.11128368456692
24-25	21.192924704067728	27.61577469035264	30.0519656002	21.139335005379635
26-27	20.957173850784486	27.60464248852445	30.120025923877385	21.318157736813674
28-29	20.986047224276422	27.565403077206245	30.253343147436755	21.195206551080574
30-31	21.294612368443907	27.86275136786755	29.870357293714523	20.972278969974024
32-33	21.017497896473756	27.658922865029577	30.17211319743823	21.151466041058438
34-35	21.1748418834144	27.455396245073427	30.15581336592794	21.21394850558423
36-37	21.442831950897876	27.71243113703607	29.659564538536642	21.185172373529408
38-39	21.285690899223948	27.81396623226951	29.776636459087275	21.123706409419267
40-41	21.16491174049372	27.62396652668461	29.865523716330504	21.345598016491163
42-43	21.181632774915975	27.64076479606968	29.735125963421684	21.442476465592662
44-45	21.31631323929178	27.77453293546474	29.521862530303842	21.387291294939637
46-47	21.383640195123686	27.92418441660402	29.187236724032132	21.50493866424016
48-49	21.407446368134334	27.876079365372114	29.03092452071024	21.685549745783312
50-51	21.435001842151575	27.90499716229087	28.88066823994368	21.779332755613876
52-53	21.391029553925268	27.96255389220523	28.893648602836713	21.75276795103279
54-55	21.49896758733101	28.218436623432297	28.68943885788481	21.59315693135188
56-57	21.387601811841513	28.017573880703534	28.7293978403917	21.865426467063255
58-59	21.234787262232736	27.704978117541053	29.060418680598016	21.9998159396282
60-61	21.43225739436637	28.038976140742527	28.726155422946555	21.802611041944548
62-63	21.261776741860455	28.061823349504344	28.77081961787037	21.905580290764835
64-65	21.272347386220066	27.794527011102073	28.895777879362218	22.037347723315644
66-67	21.208640403221448	27.87026713075558	28.70979573195781	22.21129673406516
68-69	21.517632885448716	27.99573200794802	28.58147678372577	21.905158322877497
70-71	21.501693095512277	27.852548426874108	28.570768567348882	22.074989910264737
72-73	21.326197020463955	27.972023054641276	28.595262004747124	22.106517920147645
74-75	21.445599882210278	27.96154390149829	28.485466360875606	22.107389855415832
76-77	21.50413419811817	27.992487773386713	28.299754545576228	22.20362348291889
78-79	21.543675011397738	28.0172768212682	28.35257486531051	22.086473302023553
80-81	21.4341243139424	27.92875666133655	28.36301846900337	22.274100555717684
82-83	21.374445329529372	27.96526719483085	28.451799353535723	22.208488122104054
84-85	21.46652878198748	27.8708629584406	28.311991124881757	22.35061713469016
86-87	21.523418780559073	27.94831604236879	28.235007761920162	22.293257415151977
88-89	21.339320097388544	27.87746773746939	28.286133963345012	22.497078201797052
90-91	21.284525702493113	27.780971646383477	28.316680050072733	22.617822601050676
92-93	21.36652883594871	27.87862622946896	28.18143271324109	22.573412221341236
94-95	21.223038468364113	27.709436962468008	28.35013120642863	22.717393362739248
96-97	21.273138538539403	27.679746403653187	28.32288504705584	22.72423001075157
98-99	21.305179168642105	27.767237998376228	28.179455227054113	22.74812760592756
100	21.322382828961718	27.692683675453324	28.15831820859993	22.82661528698503
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	791.0
1	585.5
2	705.5
3	1083.0
4	751.5
5	464.0
6	1063.5
7	2872.0
8	4893.5
9	5623.0
10	4557.0
11	3250.5
12	3141.5
13	3570.5
14	4138.0
15	4862.5
16	5669.5
17	6471.0
18	7638.0
19	9073.0
20	11049.5
21	13702.0
22	17397.5
23	22669.0
24	30086.5
25	40677.5
26	54464.0
27	71286.0
28	92127.0
29	116192.0
30	140069.0
31	162426.5
32	182965.0
33	202709.0
34	221538.0
35	238360.5
36	254011.0
37	269001.0
38	282008.5
39	290396.5
40	296272.0
41	300803.0
42	302027.0
43	301522.0
44	297553.5
45	291436.5
46	286203.5
47	277413.0
48	265988.0
49	257309.5
50	250140.5
51	240478.5
52	225120.5
53	210565.5
54	199657.5
55	188401.5
56	172710.0
57	153639.5
58	138009.5
59	125081.0
60	109612.0
61	91981.0
62	76810.5
63	62963.5
64	50029.0
65	39076.5
66	30306.5
67	22966.0
68	17138.5
69	12625.0
70	8839.5
71	6296.0
72	4584.0
73	3087.5
74	2119.0
75	1384.5
76	841.0
77	512.5
78	280.5
79	141.5
80	71.0
81	37.0
82	22.0
83	14.0
84	8.5
85	5.5
86	4.0
87	2.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	1.0
98	3.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.037885287888094435
2	0.0
3	0.0
4	1.1102819635065123E-4
5	0.0
6	0.0
7	0.0
8	9.499079021111271E-4
9	0.0
10-11	0.0
12-13	0.045416700540546945
14-15	1.233646626118347E-4
16-17	6.168233130591735E-6
18-19	3.700939878355041E-5
20-21	1.0485996322005949E-4
22-23	0.0
24-25	1.6654229452597684E-4
26-27	1.0485996322005949E-4
28-29	3.392528221825454E-4
30-31	1.233646626118347E-5
32-33	2.158881595707107E-4
34-35	6.168233130591735E-5
36-37	5.5514098175325614E-5
38-39	3.207481227907702E-4
40-41	1.233646626118347E-4
42-43	5.551409817532561E-4
44-45	2.7757049087662807E-4
46-47	1.3570112887301817E-4
48-49	8.697208714134346E-4
50-51	4.7495395105556353E-4
52-53	6.538327118427238E-4
54-55	4.502810185331966E-4
56-57	5.181315829697057E-4
58-59	1.603740613953851E-4
60-61	1.603740613953851E-4
62-63	0.0010177584665476361
64-65	4.8729041731674705E-4
66-67	0.0016160770802150345
68-69	0.0015975723808232594
70-71	4.7495395105556353E-4
72-73	0.0010979454972453288
74-75	9.684126015029023E-4
76-77	7.833656075851503E-4
78-79	0.0033986964549560456
80-81	0.002769536675635689
82-83	0.0013570112887301817
84-85	0.0029792566020758076
86-87	0.0015975723808232594
88-89	0.0020663580987482313
90-91	0.0029607519026840327
92-93	0.002615330847370895
94-95	0.0038304727740974672
96-97	0.0031334624303406013
98-99	0.0030841165652958674
100	0.0014927124176031998
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8106049.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.03626839489696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.76253042375937	40.77340683917636
2	15.718229151500825	17.615818148519878
3	5.158042135044384	8.671123004146034
4	2.1685541765483927	4.86070735463762
5	1.1515223082358528	3.226350656350776
6	0.7222823790482562	2.4284405549551678
7	0.46464670745253883	1.8225947325330945
8	0.3091654048189051	1.3859580482279308
9	0.237276347661037	1.1966472991165278
>10	1.2141346316330757	12.14552986111567
>50	0.06719153713021363	2.566785741922599
>100	0.0254987097369219	2.2909834096062363
>500	5.293232303856309E-4	0.18811057549637694
>1k	3.3063684246119926E-4	0.31956497082701624
>5k	2.204245245781351E-5	0.11274693666167143
>10k+	4.408490491562702E-5	0.3952318667068539
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16188	0.19970271583603802	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15810	0.19503953158931064	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9128	0.11260726403208271	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.233646626118347E-5	0.0
5	0.0	0.0	0.0	1.233646626118347E-5	0.0
6	0.0	0.0	0.0	1.233646626118347E-5	0.0
7	0.0	0.0	0.0	1.233646626118347E-5	0.0
8	0.0	0.0	0.0	1.233646626118347E-5	0.0
9	0.0	0.0	0.0	1.233646626118347E-5	0.0
10-11	0.0	0.0	0.0	3.700939878355041E-5	0.0
12-13	0.0	0.0	0.0	6.168233130591735E-5	0.0
14-15	0.0	0.0	0.0	7.401879756710082E-5	0.0
16-17	0.0	0.0	0.0	7.401879756710082E-5	0.0
18-19	0.0	0.0	0.0	7.401879756710082E-5	0.0
20-21	0.0	0.0	0.0	1.9738346017893552E-4	0.0
22-23	0.0	0.0	0.0	4.1327161974964624E-4	0.0
24-25	0.0	0.0	0.0	8.512161720216594E-4	0.0
26-27	0.0	0.0	0.0	0.0016962641109127272	0.0
28-29	0.0	0.0	0.0	0.005236829927872383	0.0
30-31	0.0	0.0	0.0	0.016956472875996678	0.0
32-33	0.0	0.0	0.0	0.043535389435716464	0.0
34-35	0.0	0.0	0.0	0.08439376569275611	0.0
36-37	0.0	0.0	0.0	0.15104769290193037	0.0
38-39	0.0	0.0	0.0	0.237156227404991	0.0
40-41	0.0	0.0	0.0	0.3262378502769968	0.0
42-43	0.0	0.0	0.0	0.41635573631494205	0.0
44-45	0.0	0.0	0.0	0.5286977663224094	0.0
46-47	0.0	0.0	0.0	0.6621166489371085	0.0
48-49	0.0	0.0	0.0	0.7994708642891253	0.0
50-51	0.0	4.934586504473388E-5	0.0	0.9342159170269018	0.0
52-53	0.0	4.934586504473388E-5	0.0	1.0682639594209213	0.0
54-55	0.0	6.168233130591735E-5	0.0	1.214864356235695	0.0
56-57	0.0	8.018703069769255E-5	0.0	1.3826834750197043	0.0
58-59	0.0	8.635526382828429E-5	0.0	1.5663302800168122	0.0
60-61	0.0	8.635526382828429E-5	0.0	1.7498475521181773	0.0
62-63	0.0	8.635526382828429E-5	0.0	1.9312059426238357	0.0
64-65	0.0	9.252349695887602E-5	0.0	2.122032570984952	0.0
66-67	0.0	9.869173008946776E-5	0.0	2.339715686396665	0.0
68-69	0.0	1.1102819635065123E-4	0.0	2.5774825688815843	0.0
70-71	0.0	1.1102819635065123E-4	0.0	2.8253221760687603	0.0
72-73	0.0	1.1102819635065123E-4	0.0	3.0785281460795515	0.0
74-75	0.0	1.1102819635065123E-4	0.0	3.333411875501863	0.0
76-77	0.0	1.1102819635065123E-4	0.0	3.6065350702913346	0.0
78-79	0.0	1.1102819635065123E-4	0.0	3.906619612094622	0.0
80-81	0.0	1.1102819635065123E-4	0.0	4.222982121129542	0.0
82-83	0.0	1.1102819635065123E-4	0.0	4.545519031528183	0.0
84-85	1.233646626118347E-5	1.1102819635065123E-4	0.0	4.866988837595233	0.0
86-87	1.233646626118347E-5	1.1102819635065123E-4	0.0	5.204236984010336	0.0
88	1.233646626118347E-5	1.1102819635065123E-4	0.0	5.469939794343706	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12380	0.0	56.734383	1
GTATCAA	22290	0.0	39.567547	1
TCAACGC	27185	0.0	31.998922	4
ATCAACG	27285	0.0	31.81275	3
CAACGCA	27990	0.0	31.128996	5
TATCAAC	28870	0.0	30.228973	2
AACGCAG	29110	0.0	30.01159	6
GTGGTAT	5435	0.0	28.458548	1
ACGCAGA	33695	0.0	25.746454	7
CGCAGAG	34440	0.0	25.025896	8
TGGTATC	5490	0.0	24.824694	2
GTACATG	21505	0.0	22.910692	1
GCAGAGT	37765	0.0	22.498327	9
TACATGG	22015	0.0	21.475216	2
GAGTACT	25945	0.0	20.932432	12-13
CAGAGTA	36830	0.0	20.227318	10-11
AGAGTAC	34825	0.0	20.189556	10-11
ACATGGG	22410	0.0	20.069122	3
AGTACTT	27325	0.0	19.08371	12-13
GTACTTT	28780	0.0	18.794905	14-15
>>END_MODULE