FastQCFastQC Report
Wed 25 May 2016
SRR2049370_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049370_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4694995
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47850.042.0497861
GTATCAA74750.033.8355371
TCAACGC81850.029.6353344
ATCAACG82200.029.566343
CAACGCA84950.028.3322325
TATCAAC91700.027.015942
AACGCAG90050.026.7752676
GTACATG149000.026.6923031
TACATGG150250.025.1547722
ACATGGG159600.022.7385773
ACGCAGA109050.021.7653627
GTGGTAT25700.021.584941
CGCAGAG114250.020.3633488
CATGGGG114900.019.9245134
GAGTACA111850.018.9137881
GCAGAGT129900.017.3311169
AGTACAT113300.017.1770762
CAGAGTA119000.016.46983510-11
AGAGTAC111750.016.00321810-11
TGGTATC32050.015.6940342
Produced by FastQC (version 0.11.5)