##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049370_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4694995 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.595851326785226 31.0 31.0 34.0 27.0 34.0 2 30.68966015938249 31.0 31.0 34.0 27.0 34.0 3 30.882473996244936 31.0 31.0 34.0 27.0 34.0 4 34.48504013316308 37.0 35.0 37.0 32.0 37.0 5 34.48064098044833 37.0 35.0 37.0 31.0 37.0 6 34.51219522065519 37.0 35.0 37.0 31.0 37.0 7 34.37216653052879 37.0 35.0 37.0 30.0 37.0 8 34.36575800400213 37.0 35.0 37.0 30.0 37.0 9 35.90274792624912 39.0 37.0 39.0 30.0 39.0 10-11 35.88709199903301 39.0 37.0 39.0 30.5 39.0 12-13 35.846151486849294 39.0 37.0 39.0 30.0 39.0 14-15 37.01536103872316 40.0 37.0 41.0 31.0 41.0 16-17 36.9731959245963 40.0 37.0 41.0 31.0 41.0 18-19 36.96827802798512 40.0 37.0 41.0 31.0 41.0 20-21 37.05251975774202 40.0 37.0 41.0 31.0 41.0 22-23 36.98347186738218 40.0 37.0 41.0 31.0 41.0 24-25 36.98319913013752 40.0 37.0 41.0 30.5 41.0 26-27 36.971663228608335 40.0 37.0 41.0 30.0 41.0 28-29 36.913220354867256 40.0 37.0 41.0 30.0 41.0 30-31 36.84970473876969 40.0 37.0 41.0 30.0 41.0 32-33 36.80325005670933 40.0 37.0 41.0 30.0 41.0 34-35 36.70847200050267 40.0 37.0 41.0 30.0 41.0 36-37 36.60951630406422 40.0 36.5 41.0 30.0 41.0 38-39 36.50219244109951 40.0 36.0 41.0 30.0 41.0 40-41 36.40085229057752 40.0 36.0 41.0 30.0 41.0 42-43 36.24014264125947 40.0 36.0 41.0 29.5 41.0 44-45 36.0761775039164 39.0 35.0 41.0 29.0 41.0 46-47 35.94438141041684 39.0 35.0 41.0 29.0 41.0 48-49 35.80482971760353 39.0 35.0 41.0 28.0 41.0 50-51 35.64373593582101 39.0 35.0 41.0 28.0 41.0 52-53 35.47331669149807 39.0 35.0 40.0 28.0 41.0 54-55 35.313364231484805 39.0 35.0 40.0 27.0 41.0 56-57 35.1517262531696 38.5 34.0 40.0 27.0 41.0 58-59 34.937837739976295 38.0 34.0 40.0 26.5 41.0 60-61 34.69469829041351 38.0 34.0 40.0 26.0 41.0 62-63 34.451435518035694 37.5 34.0 40.0 26.0 41.0 64-65 34.183835020058595 37.0 34.0 40.0 26.0 41.0 66-67 33.901480086773255 36.5 33.0 39.5 26.0 41.0 68-69 33.59222523133677 36.0 33.0 39.0 25.0 41.0 70-71 33.295692327680854 35.5 33.0 39.0 25.0 40.5 72-73 32.94184541197595 35.0 33.0 38.5 24.0 40.0 74-75 32.59448636260528 35.0 32.5 37.5 23.5 40.0 76-77 32.270618605557615 35.0 32.0 37.0 23.0 39.0 78-79 31.957203255807514 35.0 32.0 37.0 22.0 39.0 80-81 31.61931972238522 35.0 32.0 36.0 21.0 39.0 82-83 31.3158773332027 35.0 32.0 36.0 20.0 37.0 84-85 31.042217510348785 35.0 32.0 35.0 19.5 37.0 86-87 30.740456805598303 35.0 31.0 35.0 18.0 36.5 88-89 30.494251112088513 35.0 31.0 35.0 16.0 36.0 90-91 30.294522145390996 34.0 31.0 35.0 9.5 36.0 92-93 30.0567996345044 34.0 31.0 35.0 4.5 36.0 94-95 29.845777152052342 34.0 31.0 35.0 2.0 35.0 96-97 29.571454282698916 34.0 31.0 35.0 2.0 35.0 98-99 29.352057776419358 34.0 31.0 35.0 2.0 35.0 100 29.21283877831606 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5118304367850435 1101 2 0.4739224376923481 1101 3 0.5141954896665943 1101 4 0.4825009450933919 1101 5 0.4537553867015518 1101 6 0.6218885834698895 1101 7 0.570990528214196 1101 8 0.57782371946859 1101 9 0.7012970878892943 1101 10-11 0.7370500507344033 1101 12-13 0.7147606655459668 1101 14-15 0.8799868762445016 1101 16-17 0.8349715939538385 1101 18-19 0.8099049995497793 1101 20-21 0.8141638037605219 1101 22-23 0.8652154155591205 1101 24-25 0.9094344468880067 1101 26-27 0.8263985886698393 1101 28-29 0.8475370530863344 1101 30-31 0.8811062378362138 1101 32-33 0.8170113632846565 1101 34-35 0.917497226085807 1101 36-37 0.890208577877317 1101 38-39 0.9818727435386592 1101 40-41 1.0093888924355952 1101 42-43 0.9464105825448001 1101 44-45 0.9506217638106094 1101 46-47 0.9084882269763312 1101 48-49 0.8476826321096382 1101 50-51 0.892956776656753 1101 52-53 0.9498934227036671 1101 54-55 0.9653364934880742 1101 56-57 0.9036769939469025 1101 58-59 0.9615372588570068 1101 60-61 0.9620351775831111 1101 62-63 0.9786259962159747 1101 64-65 0.9057485244527257 1101 66-67 0.9713901590511682 1101 68-69 0.9260204000928098 1101 70-71 0.9723682885041853 1101 72-73 1.0086461067851147 1101 74-75 0.9652431923937215 1101 76-77 0.9767634625567112 1101 78-79 0.9511339804502335 1101 80-81 0.9131169504754695 1101 82-83 0.9534205214440838 1101 84-85 0.9295609258354176 1101 86-87 0.8956224451218127 1101 88-89 0.9123767570078627 1101 90-91 0.9656616575567121 1101 92-93 0.9821325657316358 1101 94-95 0.9920944051146527 1101 96-97 1.0123268639042884 1101 98-99 1.0499051752988713 1101 100 1.1408132895414091 1102 1 0.39361390325996837 1102 2 0.4842310076025633 1102 3 0.5264178865337215 1102 4 0.5196159417081816 1102 5 0.5033102585362528 1102 6 0.6028120080307602 1102 7 0.6852999995393461 1102 8 0.6629063820764145 1102 9 0.7898719044021618 1102 10-11 0.7363766292004286 1102 12-13 0.7746371202146918 1102 14-15 0.8577698776137481 1102 16-17 0.8211447133981835 1102 18-19 0.81223794518597 1102 20-21 0.7820286883801728 1102 22-23 0.809783883335875 1102 24-25 0.8944633671022046 1102 26-27 0.9128668604237546 1102 28-29 0.8406547980131762 1102 30-31 0.7980827695244344 1102 32-33 0.9036522826144591 1102 34-35 0.866016343357991 1102 36-37 0.8876199331967811 1102 38-39 0.9285042778561845 1102 40-41 0.9897896948519644 1102 42-43 0.9608478488534686 1102 44-45 0.9682214432978213 1102 46-47 0.8986289356233002 1102 48-49 0.8716993301014853 1102 50-51 0.9121320125282679 1102 52-53 0.9507984917337922 1102 54-55 0.925064712084243 1102 56-57 0.87824330526513 1102 58-59 0.9849531698996614 1102 60-61 0.9194962132569628 1102 62-63 0.8955413246221369 1102 64-65 0.8818068389311406 1102 66-67 0.9056401417896822 1102 68-69 0.9541895951534869 1102 70-71 0.9559894132462077 1102 72-73 0.8993946814990892 1102 74-75 0.8934302870419586 1102 76-77 0.9089618864113049 1102 78-79 0.862742293843322 1102 80-81 0.8667810156845022 1102 82-83 0.8758303905851186 1102 84-85 0.9009038497554087 1102 86-87 0.8593959705083023 1102 88-89 0.7999534615399924 1102 90-91 0.8762635363698514 1102 92-93 0.8748268943329407 1102 94-95 0.8588380403714524 1102 96-97 0.9011926659732445 1102 98-99 1.0072928564776582 1102 100 0.929355982784692 1103 1 0.3244610566267241 1103 2 0.43328372450044483 1103 3 0.422672863305273 1103 4 0.4412209679207919 1103 5 0.42782823982367546 1103 6 0.5050248431488384 1103 7 0.5088876838685152 1103 8 0.4541325119078792 1103 9 0.6304290414908778 1103 10-11 0.610411601268126 1103 12-13 0.6187011478612163 1103 14-15 0.7231694608420582 1103 16-17 0.7197128510171353 1103 18-19 0.7599212121904486 1103 20-21 0.693953288025611 1103 22-23 0.7212523544396205 1103 24-25 0.7153052602437668 1103 26-27 0.7280987002128043 1103 28-29 0.7076795189641274 1103 30-31 0.7103254694867545 1103 32-33 0.7417759016999668 1103 34-35 0.7384387697886083 1103 36-37 0.7569192168282939 1103 38-39 0.8319137615195586 1103 40-41 0.7889320526467571 1103 42-43 0.8286197493880039 1103 44-45 0.7020252921135537 1103 46-47 0.7532777544751283 1103 48-49 0.8266069558263496 1103 50-51 0.7539506763804837 1103 52-53 0.7498967192739414 1103 54-55 0.7398141222056296 1103 56-57 0.7504913181391899 1103 58-59 0.7766226886649221 1103 60-61 0.7706497642040091 1103 62-63 0.8171642537245916 1103 64-65 0.8065151661879568 1103 66-67 0.8451322202614548 1103 68-69 0.849668319902932 1103 70-71 0.7947126579621653 1103 72-73 0.8397354665371211 1103 74-75 0.8271349481578554 1103 76-77 0.9084221021648347 1103 78-79 0.8043228987860793 1103 80-81 0.7755745273990442 1103 82-83 0.7772040870144927 1103 84-85 0.8288830212867992 1103 86-87 0.85858272199048 1103 88-89 0.8319569793155885 1103 90-91 0.8762627249969732 1103 92-93 0.7918958719862594 1103 94-95 0.9138369613508992 1103 96-97 0.9438326183108856 1103 98-99 0.9469752809188243 1103 100 0.8919842745349875 1104 1 0.3375733660607203 1104 2 0.40877300518819126 1104 3 0.38903052408685923 1104 4 0.42179032155524965 1104 5 0.49474822518250505 1104 6 0.5240271057278534 1104 7 0.5038893601528969 1104 8 0.5644572899823999 1104 9 0.6474776634555468 1104 10-11 0.6852662827983877 1104 12-13 0.6375013857802259 1104 14-15 0.768094003750889 1104 16-17 0.7622406919142719 1104 18-19 0.7095793959403665 1104 20-21 0.7529358441406515 1104 22-23 0.7316133305392114 1104 24-25 0.809090820551944 1104 26-27 0.7319585474522867 1104 28-29 0.7120716067398831 1104 30-31 0.73073291591448 1104 32-33 0.7172658504720744 1104 34-35 0.7477111327989192 1104 36-37 0.7218646485079603 1104 38-39 0.729060454218569 1104 40-41 0.7577069843401887 1104 42-43 0.7863654586668503 1104 44-45 0.7151932954887954 1104 46-47 0.6690844481135159 1104 48-49 0.669982109890654 1104 50-51 0.7539627423888362 1104 52-53 0.7896666674629884 1104 54-55 0.8136189800291831 1104 56-57 0.8079148285540185 1104 58-59 0.8343812535063577 1104 60-61 0.7373403536892482 1104 62-63 0.7480948790353494 1104 64-65 0.8015207699985538 1104 66-67 0.8565559566102579 1104 68-69 0.8062362976885211 1104 70-71 0.8265483351243716 1104 72-73 0.8243947111240217 1104 74-75 0.8951990178524127 1104 76-77 0.9252449116174617 1104 78-79 0.9210871192652625 1104 80-81 0.943012095928303 1104 82-83 0.8943389323509372 1104 84-85 0.8815341419679399 1104 86-87 0.8887150674767419 1104 88-89 0.8749655372737308 1104 90-91 0.9406026698010592 1104 92-93 0.9205968366190866 1104 94-95 1.0192849260825234 1104 96-97 1.0052228289720553 1104 98-99 0.9689306990424562 1104 100 0.9435655694687206 1105 1 0.28310900975048625 1105 2 0.362287208545105 1105 3 0.35981950991229184 1105 4 0.39901255870390884 1105 5 0.3490274638319093 1105 6 0.39253929401589005 1105 7 0.4211261825619985 1105 8 0.41085196712998595 1105 9 0.5327863144341052 1105 10-11 0.5172086556667281 1105 12-13 0.4333062234808267 1105 14-15 0.556095266527386 1105 16-17 0.5362317757142634 1105 18-19 0.48713991517564637 1105 20-21 0.494908826864787 1105 22-23 0.5115675021156036 1105 24-25 0.5598614404638909 1105 26-27 0.5450099758482523 1105 28-29 0.5953477824845947 1105 30-31 0.6092911437285764 1105 32-33 0.5984995140208156 1105 34-35 0.6535642745791677 1105 36-37 0.6229582935564579 1105 38-39 0.6273257102090852 1105 40-41 0.6508548097930031 1105 42-43 0.6861997240495015 1105 44-45 0.6332175876238892 1105 46-47 0.5531619495816287 1105 48-49 0.6057363405757314 1105 50-51 0.5960624825409084 1105 52-53 0.6321108692462261 1105 54-55 0.6423384709268234 1105 56-57 0.5983446715321747 1105 58-59 0.6033461030286844 1105 60-61 0.541386362509499 1105 62-63 0.5349061572798988 1105 64-65 0.5686384538591938 1105 66-67 0.6052994939965046 1105 68-69 0.6167276674363862 1105 70-71 0.6265219856999096 1105 72-73 0.5914572972442329 1105 74-75 0.6366226352272903 1105 76-77 0.6451431410436115 1105 78-79 0.5642009572230187 1105 80-81 0.628919606185967 1105 82-83 0.5760046650491724 1105 84-85 0.6328952154431065 1105 86-87 0.6341936175483234 1105 88-89 0.6097853901161301 1105 90-91 0.6800633030316554 1105 92-93 0.7015500409624238 1105 94-95 0.6909428287988355 1105 96-97 0.7262162195461421 1105 98-99 0.7525871662620816 1105 100 0.7739543434240517 1106 1 0.2707662326645597 1106 2 0.23488802097468664 1106 3 0.3037322182085589 1106 4 0.28649847461448275 1106 5 0.2788279145227719 1106 6 0.35710098232757304 1106 7 0.3090745754932698 1106 8 0.3127195173879471 1106 9 0.4245984919825858 1106 10-11 0.4469802710376669 1106 12-13 0.43665556820003104 1106 14-15 0.5403961768043217 1106 16-17 0.5496896420488468 1106 18-19 0.5900522083619109 1106 20-21 0.583612238179505 1106 22-23 0.5963712143850728 1106 24-25 0.5535324954363148 1106 26-27 0.5128544430249136 1106 28-29 0.5472293056418067 1106 30-31 0.5643056355153249 1106 32-33 0.5852691798572636 1106 34-35 0.68408286557721 1106 36-37 0.5745322276132683 1106 38-39 0.59564247620564 1106 40-41 0.6353488944754417 1106 42-43 0.6547965410452861 1106 44-45 0.6255657389018339 1106 46-47 0.539334153519242 1106 48-49 0.665899864266656 1106 50-51 0.6697787548530698 1106 52-53 0.6740996538634363 1106 54-55 0.7052867156045224 1106 56-57 0.6178762273051746 1106 58-59 0.7320347260494984 1106 60-61 0.7247075019864582 1106 62-63 0.6278869065534565 1106 64-65 0.6410525038502968 1106 66-67 0.666502534677285 1106 68-69 0.6955720308355424 1106 70-71 0.6662901171118847 1106 72-73 0.7032004998179104 1106 74-75 0.6834357764239982 1106 76-77 0.7640156355582306 1106 78-79 0.6856454659106781 1106 80-81 0.6444725549495303 1106 82-83 0.6393407040102126 1106 84-85 0.6005694875180652 1106 86-87 0.6702976989691827 1106 88-89 0.635291157180113 1106 90-91 0.6738836493520033 1106 92-93 0.6787274411877675 1106 94-95 0.727528141317368 1106 96-97 0.6874526543442165 1106 98-99 0.7307117215013612 1106 100 0.7826883320381768 1107 1 0.17496747811177116 1107 2 0.12968406364313978 1107 3 0.1953403635811597 1107 4 0.20083345620544435 1107 5 0.18273644437241643 1107 6 0.28438656478760294 1107 7 0.25516379762275676 1107 8 0.28479757041854015 1107 9 0.3285531098976051 1107 10-11 0.2559566620169491 1107 12-13 0.2700751847938818 1107 14-15 0.2950275726783289 1107 16-17 0.36534858425390127 1107 18-19 0.3756639144831624 1107 20-21 0.3413115459636771 1107 22-23 0.3706430011266164 1107 24-25 0.36895994241794483 1107 26-27 0.33560683683975157 1107 28-29 0.36080386325458136 1107 30-31 0.3335047340662314 1107 32-33 0.34020454962104907 1107 34-35 0.400121163122634 1107 36-37 0.38449389194606454 1107 38-39 0.45338060418190906 1107 40-41 0.4775313909920982 1107 42-43 0.45193668383026164 1107 44-45 0.3537952027583131 1107 46-47 0.36560177174622766 1107 48-49 0.39729228984771225 1107 50-51 0.4068807169315036 1107 52-53 0.48974351832335117 1107 54-55 0.4518934772438854 1107 56-57 0.4533884857847923 1107 58-59 0.5153721116405166 1107 60-61 0.39466208105218925 1107 62-63 0.3994827300071435 1107 64-65 0.44497308498333865 1107 66-67 0.4357807140917558 1107 68-69 0.42114099109461023 1107 70-71 0.3832037605178584 1107 72-73 0.4225958600483395 1107 74-75 0.42471272793891757 1107 76-77 0.5111095593251491 1107 78-79 0.4185403915237309 1107 80-81 0.44734540557967506 1107 82-83 0.41450560616237553 1107 84-85 0.4348006105448974 1107 86-87 0.4648365396771901 1107 88-89 0.4502408815392904 1107 90-91 0.4428664759275982 1107 92-93 0.42502315385548783 1107 94-95 0.38718616641721226 1107 96-97 0.3240166482959488 1107 98-99 0.37936117385728707 1107 100 0.4303596224981874 1108 1 0.14397604467767522 1108 2 0.18102571111968402 1108 3 0.21586843700295333 1108 4 0.20103612394829895 1108 5 0.23274240667315382 1108 6 0.32063906604216896 1108 7 0.26557519709463406 1108 8 0.25157643122641815 1108 9 0.2667717028068921 1108 10-11 0.3059654549231041 1108 12-13 0.369767386201886 1108 14-15 0.3446759051109751 1108 16-17 0.35124939270005484 1108 18-19 0.2862996547602208 1108 20-21 0.36305607657591565 1108 22-23 0.3622584774059021 1108 24-25 0.3259893868083239 1108 26-27 0.3427082128637622 1108 28-29 0.38804532143393544 1108 30-31 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2216 3 -2.056731166209808 2216 4 -2.153894546084004 2216 5 -2.174332885020661 2216 6 -3.473312796016266 2216 7 -3.2923641050651895 2216 8 -4.063762401015197 2216 9 -3.8580316391403144 2216 10-11 -3.132930312751533 2216 12-13 -2.9039177116819275 2216 14-15 -3.29629274386771 2216 16-17 -3.569715015458712 2216 18-19 -3.3616296306993547 2216 20-21 -3.1203946572562558 2216 22-23 -3.4256381535246163 2216 24-25 -3.547722359033969 2216 26-27 -3.59599870141907 2216 28-29 -3.992487525624462 2216 30-31 -3.716055726729074 2216 32-33 -3.731640775808728 2216 34-35 -4.520556546457705 2216 36-37 -3.733184727189702 2216 38-39 -3.676846205396785 2216 40-41 -4.239835627594125 2216 42-43 -4.040238632114885 2216 44-45 -3.68069709325529 2216 46-47 -3.618188752968692 2216 48-49 -3.7454573342689983 2216 50-51 -4.062359752406017 2216 52-53 -4.288593002488788 2216 54-55 -3.796621578405862 2216 56-57 -3.7689792504164643 2216 58-59 -4.479205982598764 2216 60-61 -5.001733049022018 2216 62-63 -4.391737898571748 2216 64-65 -4.3462179527831 2216 66-67 -4.111266818825587 2216 68-69 -4.527765791577842 2216 70-71 -4.228876640032368 2216 72-73 -4.51405474962182 2216 74-75 -4.922387847968761 2216 76-77 -5.613601298017308 2216 78-79 -4.749166218921108 2216 80-81 -4.511281688797077 2216 82-83 -4.73327186228202 2216 84-85 -4.836306652151485 2216 86-87 -4.497104228551311 2216 88-89 -4.424801008157228 2216 90-91 -4.549084606936187 2216 92-93 -4.630892587765057 2216 94-95 -4.579736817062944 2216 96-97 -4.920086912198567 2216 98-99 -4.891346234610051 2216 100 -4.648355483471072 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 82843.0 3 15118.0 4 9830.0 5 8067.0 6 7468.0 7 7684.0 8 8602.0 9 9718.0 10 10549.0 11 11715.0 12 12576.0 13 13887.0 14 14881.0 15 15216.0 16 15749.0 17 16683.0 18 16031.0 19 15275.0 20 15256.0 21 16346.0 22 18539.0 23 22490.0 24 28179.0 25 35419.0 26 42672.0 27 48706.0 28 59683.0 29 74010.0 30 89753.0 31 107808.0 32 138034.0 33 181563.0 34 252203.0 35 368315.0 36 606164.0 37 980455.0 38 1048064.0 39 269115.0 40 329.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.443076737592065 19.353135982798804 13.568095988609787 23.635691290999343 2 15.984252958269446 19.860549454833887 39.516569739014386 24.638627847882283 3 18.768234727144353 24.66464294129904 29.758115958270466 26.80900637328614 4 13.042849274908411 16.290351962109664 37.46211019602454 33.20468856695739 5 13.710343070181755 37.69327499945669 34.72121313100279 13.875168799358764 6 32.12483620441689 36.65098490417292 17.282197151288525 13.941981740121662 7 28.245246444287915 32.10962378505802 21.635288788550135 18.009840982103935 8 25.79280183932967 35.92498821229334 19.905915670970327 18.376294277406657 9 25.63332585480534 16.780367990451346 20.579588050292354 37.00671810445096 10-11 24.663491582575517 26.621584392955327 28.125008655736895 20.58991536873226 12-13 24.839696896124526 24.111414931836357 28.209969181023165 22.83891899101595 14-15 22.33941903311956 26.156378660445778 25.206956654023827 26.29724565241083 16-17 22.26179167672918 29.42949357051996 25.926865350307033 22.38184940244383 18-19 22.24354981478978 28.573903144392382 27.571602172005438 21.6109448688124 20-21 23.152136245159642 27.71001179188877 27.284794301371875 21.853057661579715 22-23 22.997247221654053 27.482954932842436 27.22153686848026 22.298260977023247 24-25 22.710957359296245 27.636411709899956 27.16798332696646 22.484647603837338 26-27 22.424945579699507 27.688124219542477 27.24761646860454 22.639313732153475 28-29 22.5314512275071 27.685868887365757 27.37110626804476 22.411573617082382 30-31 22.71115625597564 27.824287996301294 27.064774772519417 22.399780975203644 32-33 22.52967399591631 27.733746269211622 27.292118506148498 22.444461228723572 34-35 22.706518920740855 27.498389812579084 27.321966961915635 22.47312430476443 36-37 22.81081850379135 27.637559179771536 27.044640267203935 22.50698204923318 38-39 22.650254514836686 27.824313602503477 27.120200629107156 22.40523125355268 40-41 22.58883924671733 27.558127147876142 27.31211077235123 22.540922833055294 42-43 22.514462271113466 27.616855065374242 27.27268369300101 22.595998970511285 44-45 22.510309152055598 27.5923828921872 27.282889950134702 22.614418005622497 46-47 22.554501536642395 27.604744683004256 27.196045742408664 22.64470803794468 48-49 22.560389648642335 27.580113576502963 27.170657382946732 22.688839391907965 50-51 22.51029028845405 27.583480527681754 27.150638062333275 22.75559112153092 52-53 22.516161361785684 27.568624267673314 27.236065179791552 22.679149190749452 54-55 22.525536189131106 27.71517287355019 27.14400550703474 22.61528543028396 56-57 22.50012996188069 27.573170669350556 27.17091734339167 22.75578202537708 58-59 22.48919554335471 27.32703548293285 27.281910830875233 22.901858142837206 60-61 22.608894531936176 27.561744443034748 27.108081364276636 22.72127966075244 62-63 22.435990987871975 27.61695260110681 27.107746062520306 22.83931034850091 64-65 22.511830519971912 27.481245440786466 27.137717223089563 22.869206816152058 66-67 22.405174710500695 27.430271378932474 27.14326208860058 23.02129182196625 68-69 22.506369968539776 27.537768941355385 27.0624407650716 22.893420325033244 70-71 22.48533234817505 27.377599835693324 27.20353532060894 22.933532495522684 72-73 22.40203946586311 27.449103898820947 27.223986733075144 22.924869902240797 74-75 22.42480919876798 27.465321504576607 27.175777452570532 22.934091844084882 76-77 22.483782878320056 27.351950022663225 27.085839559284175 23.078427539732544 78-79 22.421685359589176 27.434084398686288 27.180649509593742 22.963580732130794 80-81 22.403724048905037 27.39411703986304 27.192900879975824 23.009258031256095 82-83 22.338965808346714 27.356454443530108 27.256758304238872 23.047821443884303 84-85 22.385847315921502 27.27729333070145 27.211141173709706 23.12571817966734 86-87 22.527510045658065 27.30615509807892 27.175351953562384 22.990982902700637 88-89 22.428114614374582 27.24536169540065 27.22928691239957 23.097236777825195 90-91 22.382809849319226 27.176942450829202 27.263292891939763 23.176954807911816 92-93 22.447228662568637 27.234010142280614 27.1792028386881 23.139558356462654 94-95 22.393515277358183 27.059994315758345 27.289270802130016 23.257219604753455 96-97 22.47999199147177 27.013095860591974 27.27926227823459 23.227649869701672 98-99 22.425433892900845 27.114533668299966 27.20603536318995 23.253997075609238 100 22.430630916539847 27.018601723750507 27.26407589358455 23.2866914661251 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2677.0 1 2175.0 2 1839.0 3 1915.0 4 1640.5 5 1307.5 6 1082.0 7 1013.0 8 1047.5 9 1022.5 10 891.5 11 788.5 12 784.0 13 832.5 14 916.5 15 995.5 16 1132.0 17 1411.0 18 1778.5 19 2312.5 20 3108.5 21 4269.0 22 5932.0 23 8388.5 24 11798.5 25 16335.0 26 22774.5 27 30591.5 28 39918.5 29 50822.0 30 61431.5 31 71286.5 32 80331.5 33 89192.0 34 98958.0 35 109250.5 36 119763.5 37 131320.5 38 142215.5 39 149721.5 40 156035.5 41 163315.5 42 168591.5 43 172161.5 44 172807.5 45 171965.0 46 171381.5 47 167668.5 48 163171.5 49 159891.5 50 157043.5 51 152650.5 52 145195.0 53 138684.5 54 134103.0 55 128370.5 56 119510.0 57 108431.0 58 99327.0 59 92531.0 60 83521.5 61 72392.5 62 62856.5 63 53686.5 64 44485.5 65 36506.0 66 29440.0 67 23375.0 68 18507.5 69 14424.5 70 10925.0 71 8048.0 72 5968.5 73 4254.0 74 3019.5 75 2181.5 76 1501.0 77 1002.0 78 669.0 79 449.0 80 339.0 81 224.0 82 133.0 83 110.0 84 99.5 85 81.5 86 65.5 87 41.5 88 36.0 89 32.5 90 25.5 91 18.5 92 13.0 93 14.5 94 12.0 95 9.5 96 10.5 97 9.5 98 8.5 99 11.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009329083417554226 2 0.02743346904522795 3 0.028562330737306428 4 0.03088395195309047 5 0.03307777750562035 6 0.035037311008850915 7 0.03324817172329257 8 0.03292868256515715 9 0.033184273891665485 10-11 0.03356766088142799 12-13 0.03451547871722973 14-15 0.033237522084688054 16-17 0.030234323998215122 18-19 0.03289673364934361 20-21 0.03225775533307277 22-23 0.033120376060038405 24-25 0.030415367854491858 26-27 0.030543163517746028 28-29 0.0310543461707627 30-31 0.031150192918203323 32-33 0.030873302314485956 34-35 0.030713557735418246 36-37 0.028785973148001222 38-39 0.02894571772706893 40-41 0.029925484478684213 42-43 0.02988288592426616 44-45 0.02875402423218768 46-47 0.028115045915916844 48-49 0.028338688326611638 50-51 0.029190659414972755 52-53 0.02966989315217588 54-55 0.029009615558696016 56-57 0.027923352421035594 58-59 0.028328038688007123 60-61 0.027976600614058163 62-63 0.028115045915916844 64-65 0.02562303048246058 66-67 0.026506950486635237 68-69 0.027486717238250524 70-71 0.028391936519634207 72-73 0.025921220363386968 74-75 0.026325906630358498 76-77 0.02699683386244288 78-79 0.027358921574996354 80-81 0.02740152012941441 82-83 0.026251359160126903 84-85 0.027060731694069962 86-87 0.0276997100103408 88-89 0.02785945458940851 90-91 0.028338688326611638 92-93 0.027209826634533155 94-95 0.02805114808428976 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4694995.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.726566056491116 #Duplication Level Percentage of deduplicated Percentage of total 1 76.19584601574313 43.98524538258021 2 13.243905074718986 15.290503222833271 3 4.36357353814251 7.556823482758207 4 1.9822205687255072 4.577071463962735 5 1.1019688868108943 3.1806439868343537 6 0.6556669799715908 2.270964193823404 7 0.46137293414113695 1.864343261156283 8 0.3321646230876386 1.5339778445038434 9 0.2516016336680832 1.3071688493275513 >10 1.3201455590173552 13.703422294538218 >50 0.07076500260636336 2.7472286515332915 >100 0.020547442324485642 1.8418250283151483 >500 1.8478428513217092E-4 0.06713782960735779 >1k 3.695675711692921E-5 0.07364450822619074 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.1299277209027912E-5 0.0 3 0.0 2.1299277209027912E-5 0.0 4.2598554418055825E-5 0.0 4 0.0 2.1299277209027912E-5 0.0 4.2598554418055825E-5 0.0 5 0.0 4.2598554418055825E-5 0.0 4.2598554418055825E-5 0.0 6 0.0 4.2598554418055825E-5 0.0 4.2598554418055825E-5 0.0 7 0.0 4.2598554418055825E-5 0.0 4.2598554418055825E-5 0.0 8 0.0 4.2598554418055825E-5 0.0 4.2598554418055825E-5 0.0 9 2.1299277209027912E-5 4.2598554418055825E-5 0.0 4.2598554418055825E-5 0.0 10-11 4.2598554418055825E-5 4.2598554418055825E-5 0.0 4.2598554418055825E-5 0.0 12-13 4.2598554418055825E-5 4.2598554418055825E-5 0.0 4.2598554418055825E-5 0.0 14-15 4.2598554418055825E-5 7.45474702315977E-5 0.0 4.2598554418055825E-5 0.0 16-17 5.3248193022569786E-5 1.2779566325416748E-4 0.0 4.2598554418055825E-5 0.0 18-19 6.389783162708374E-5 1.2779566325416748E-4 0.0 7.45474702315977E-5 0.0 20-21 6.389783162708374E-5 1.2779566325416748E-4 0.0 1.490949404631954E-4 0.0 22-23 6.389783162708374E-5 1.3844530185868145E-4 0.0 4.366351827850722E-4 0.0 24-25 6.389783162708374E-5 1.490949404631954E-4 0.0 0.0010117156674288258 0.0 26-27 6.389783162708374E-5 1.490949404631954E-4 0.0 0.0018849860329989703 0.0 28-29 6.389783162708374E-5 1.490949404631954E-4 0.0 0.005953147979923301 0.0 30-31 6.389783162708374E-5 1.490949404631954E-4 0.0 0.017827495023956363 0.0 32-33 1.0649638604513956E-4 1.490949404631954E-4 0.0 0.042545306225033254 0.0 34-35 1.0649638604513956E-4 1.490949404631954E-4 0.0 0.08297133436776823 0.0 36-37 1.0649638604513956E-4 1.490949404631954E-4 0.0 0.146144990569745 0.0 38-39 1.0649638604513956E-4 1.490949404631954E-4 0.0 0.23025583626819623 0.0 40-41 1.916934948812512E-4 1.490949404631954E-4 0.0 0.31530385016384466 0.0 42-43 1.916934948812512E-4 1.490949404631954E-4 0.0 0.4003838129753067 2.1299277209027912E-5 44-45 2.0234313348576515E-4 1.490949404631954E-4 0.0 0.5043775339483855 2.1299277209027912E-5 46-47 2.5559132650833496E-4 1.490949404631954E-4 0.0 0.6284138747751595 2.1299277209027912E-5 48-49 2.5559132650833496E-4 1.5974457906770933E-4 0.0 0.7558687495939825 2.1299277209027912E-5 50-51 2.5559132650833496E-4 1.703942176722233E-4 0.0 0.8805227694598183 2.1299277209027912E-5 52-53 2.981898809263908E-4 1.703942176722233E-4 0.0 1.002333335818249 2.1299277209027912E-5 54-55 2.981898809263908E-4 1.703942176722233E-4 0.0 1.1371364612741868 2.1299277209027912E-5 56-57 2.981898809263908E-4 1.703942176722233E-4 0.0 1.2989257709539626 2.1299277209027912E-5 58-59 2.981898809263908E-4 1.703942176722233E-4 0.0 1.4667001775294755 2.1299277209027912E-5 60-61 2.981898809263908E-4 1.703942176722233E-4 0.0 1.635773840014739 2.1299277209027912E-5 62-63 2.981898809263908E-4 1.703942176722233E-4 0.0 1.8043682687627995 2.1299277209027912E-5 64-65 3.1948915813541866E-4 1.703942176722233E-4 0.0 1.9812587659837764 2.1299277209027912E-5 66-67 3.1948915813541866E-4 1.703942176722233E-4 0.0 2.184389972726276 2.1299277209027912E-5 68-69 3.1948915813541866E-4 2.3429204929930703E-4 0.0 2.4058492075071434 2.1299277209027912E-5 70-71 3.1948915813541866E-4 2.3429204929930703E-4 0.0 2.636030496305108 2.1299277209027912E-5 72-73 3.1948915813541866E-4 2.3429204929930703E-4 0.0 2.8708337282574314 2.1299277209027912E-5 74-75 3.1948915813541866E-4 2.3429204929930703E-4 0.0 3.102442068628401 2.1299277209027912E-5 76-77 3.1948915813541866E-4 2.8754024232187684E-4 0.0 3.3539971821056254 2.1299277209027912E-5 78-79 3.1948915813541866E-4 2.981898809263908E-4 0.0 3.6345299622257317 2.1299277209027912E-5 80-81 3.1948915813541866E-4 2.981898809263908E-4 0.0 3.933401420022811 2.1299277209027912E-5 82-83 3.1948915813541866E-4 2.981898809263908E-4 0.0 4.237906536641679 2.1299277209027912E-5 84-85 3.1948915813541866E-4 3.088395195309047E-4 0.0 4.536383957810392 2.1299277209027912E-5 86-87 3.1948915813541866E-4 3.1948915813541866E-4 0.0 4.853956180996998 2.1299277209027912E-5 88 3.1948915813541866E-4 3.1948915813541866E-4 0.0 5.107225886289549 2.1299277209027912E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4785 0.0 42.049786 1 GTATCAA 7475 0.0 33.835537 1 TCAACGC 8185 0.0 29.635334 4 ATCAACG 8220 0.0 29.56634 3 CAACGCA 8495 0.0 28.332232 5 TATCAAC 9170 0.0 27.01594 2 AACGCAG 9005 0.0 26.775267 6 GTACATG 14900 0.0 26.692303 1 TACATGG 15025 0.0 25.154772 2 ACATGGG 15960 0.0 22.738577 3 ACGCAGA 10905 0.0 21.765362 7 GTGGTAT 2570 0.0 21.58494 1 CGCAGAG 11425 0.0 20.363348 8 CATGGGG 11490 0.0 19.924513 4 GAGTACA 11185 0.0 18.913788 1 GCAGAGT 12990 0.0 17.331116 9 AGTACAT 11330 0.0 17.177076 2 CAGAGTA 11900 0.0 16.469835 10-11 AGAGTAC 11175 0.0 16.003218 10-11 TGGTATC 3205 0.0 15.694034 2 >>END_MODULE