FastQCFastQC Report
Wed 25 May 2016
SRR2049370_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049370_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4694995
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125040.26632616222168504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117160.24954233178097102No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72800.15505873808172319No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77550.056.4386671
GTATCAA134050.039.5946041
TCAACGC168150.031.1077374
ATCAACG168250.031.0892473
GTGGTAT31650.030.3015841
TATCAAC173600.030.1852842
CAACGCA173450.030.1568745
AACGCAG180500.029.0350346
GTACATG145450.025.1140171
ACGCAGA211400.024.5462367
TGGTATC37350.023.9074422
CGCAGAG216000.023.8056898
TACATGG147550.023.5064622
ACATGGG151050.022.1839523
GCAGAGT234300.021.5244589
CAGAGTA224200.020.11250910-11
GAGTACT153950.019.8679412-13
AGAGTAC215550.019.58827410-11
GTACTTT165150.018.45407514-15
AGTACTT156250.018.3868112-13
Produced by FastQC (version 0.11.5)