##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049370_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4694995
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.772196988495196	31.0	31.0	34.0	30.0	34.0
2	31.826030059669925	31.0	31.0	34.0	30.0	34.0
3	31.821911205443243	33.0	31.0	34.0	30.0	34.0
4	35.5905186267504	37.0	35.0	37.0	33.0	37.0
5	35.69822140385666	37.0	35.0	37.0	33.0	37.0
6	35.77175950134132	37.0	35.0	37.0	33.0	37.0
7	35.742290460373226	37.0	35.0	37.0	33.0	37.0
8	35.751636157227004	37.0	35.0	37.0	33.0	37.0
9	37.476946407823654	39.0	37.0	39.0	34.0	39.0
10-11	37.440923685754726	39.0	37.0	39.0	34.5	39.0
12-13	37.39294642486307	39.0	37.0	39.0	34.0	39.0
14-15	38.68266600922898	40.0	38.0	41.0	34.0	41.0
16-17	38.7126347312404	40.0	38.0	41.0	34.5	41.0
18-19	38.76757291541311	40.0	38.0	41.0	34.5	41.0
20-21	38.789687209464546	40.0	38.0	41.0	34.5	41.0
22-23	38.75690389446635	40.0	38.0	41.0	34.0	41.0
24-25	38.733162122643364	40.0	38.0	41.0	34.0	41.0
26-27	38.6210522055934	40.0	38.0	41.0	34.0	41.0
28-29	38.538529966485584	40.0	38.0	41.0	34.0	41.0
30-31	38.43954477054821	40.0	38.0	41.0	34.0	41.0
32-33	38.31391726721753	40.0	38.0	41.0	34.0	41.0
34-35	38.21524953700696	40.0	38.0	41.0	34.0	41.0
36-37	38.10995411070725	40.0	38.0	41.0	33.5	41.0
38-39	38.02429576602318	40.0	37.5	41.0	33.0	41.0
40-41	37.91788958241702	40.0	37.0	41.0	33.0	41.0
42-43	37.781370054707196	40.0	37.0	41.0	33.0	41.0
44-45	37.61116572009129	40.0	37.0	41.0	33.0	41.0
46-47	37.38554652347872	40.0	37.0	41.0	32.0	41.0
48-49	37.18015162955445	40.0	37.0	41.0	32.0	41.0
50-51	37.02345636150837	40.0	36.0	41.0	31.0	41.0
52-53	36.87379549924973	39.5	36.0	41.0	31.0	41.0
54-55	36.718432075007534	39.0	35.5	41.0	31.0	41.0
56-57	36.55466555342444	39.0	35.0	41.0	31.0	41.0
58-59	36.34529717284044	39.0	35.0	41.0	31.0	41.0
60-61	36.14554616139101	39.0	35.0	41.0	30.0	41.0
62-63	35.92228330381609	38.0	35.0	40.0	30.0	41.0
64-65	35.67364214445383	37.5	35.0	40.0	30.0	41.0
66-67	35.38462447776835	37.0	35.0	40.0	29.5	41.0
68-69	35.082126818026424	37.0	34.0	39.5	29.0	41.0
70-71	34.743991633643915	36.0	34.0	39.0	29.0	41.0
72-73	34.44398204896917	36.0	34.0	39.0	29.0	41.0
74-75	34.13819226644544	35.0	34.0	38.5	29.0	40.0
76-77	33.818200764857046	35.0	34.0	37.0	29.0	39.5
78-79	33.49790340564793	35.0	34.0	37.0	28.5	39.0
80-81	33.2057258846921	35.0	34.0	36.5	28.0	39.0
82-83	32.930980757168	35.0	33.0	36.0	28.0	37.5
84-85	32.69083971335433	35.0	33.0	36.0	27.0	37.0
86-87	32.48252362356084	35.0	33.0	35.0	27.0	37.0
88-89	32.27511179458125	35.0	33.0	35.0	27.0	36.0
90-91	32.10074739163727	35.0	33.0	35.0	27.0	36.0
92-93	31.94275329366698	35.0	33.0	35.0	26.0	36.0
94-95	31.7972457904641	35.0	33.0	35.0	26.0	36.0
96-97	31.61318904492976	35.0	33.0	35.0	25.5	35.0
98-99	31.466162370779948	35.0	33.0	35.0	25.0	35.0
100	31.353299204791487	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.23606789480094648
1101	2	0.23934769561042657
1101	3	0.4162475475241898
1101	4	0.29815387214666345
1101	5	0.2609516956484157
1101	6	0.210652048717769
1101	7	0.09055333688539235
1101	8	0.17318157392193
1101	9	0.2707729307765021
1101	10-11	0.28526946395825803
1101	12-13	0.24895762774644936
1101	14-15	0.34436477946097455
1101	16-17	0.29554376348482236
1101	18-19	0.3776404961415736
1101	20-21	0.3736383100079408
1101	22-23	0.3138229383590172
1101	24-25	0.3166869185299035
1101	26-27	0.40028169934370794
1101	28-29	0.4510553322831967
1101	30-31	0.38404228415535613
1101	32-33	0.3284624475473308
1101	34-35	0.33486301804514085
1101	36-37	0.33713561033138717
1101	38-39	0.3546669252263257
1101	40-41	0.30727243340161436
1101	42-43	0.27379919108300754
1101	44-45	0.28078945124609334
1101	46-47	0.29038219309181557
1101	48-49	0.33545633233023864
1101	50-51	0.22578187684960938
1101	52-53	0.3133884896155834
1101	54-55	0.27503752920831914
1101	56-57	0.3075117916213159
1101	58-59	0.35071152898409963
1101	60-61	0.35062995202129343
1101	62-63	0.3363836135218392
1101	64-65	0.28327322904407026
1101	66-67	0.2735054257818632
1101	68-69	0.2858335210327212
1101	70-71	0.27391067364477806
1101	72-73	0.21906022336987974
1101	74-75	0.27328908330164836
1101	76-77	0.2427134141247862
1101	78-79	0.2422242328920774
1101	80-81	0.34235575610328794
1101	82-83	0.24176121200170542
1101	84-85	0.3134490825266312
1101	86-87	0.22147320203992393
1101	88-89	0.27740067682307057
1101	90-91	0.34516888326071893
1101	92-93	0.33350181771010057
1101	94-95	0.3422650051220444
1101	96-97	0.3530393747902636
1101	98-99	0.3805442094615401
1101	100	0.3760963285275416
1102	1	0.2848147655090969
1102	2	0.24117050845480037
1102	3	0.43212789581678734
1102	4	0.25028880427711897
1102	5	0.2296464186732905
1102	6	0.21339543047888299
1102	7	0.19183561708996422
1102	8	0.21269199565955432
1102	9	0.2245728845059034
1102	10-11	0.27366065971724396
1102	12-13	0.24560660898067965
1102	14-15	0.334717185362976
1102	16-17	0.276918233655401
1102	18-19	0.2974360874327999
1102	20-21	0.2576105546303964
1102	22-23	0.2831826512413471
1102	24-25	0.2865346760475376
1102	26-27	0.36672732446525913
1102	28-29	0.3604701881118828
1102	30-31	0.2711247048296954
1102	32-33	0.33619599653379595
1102	34-35	0.3109332110432774
1102	36-37	0.33377025880628963
1102	38-39	0.40463730528114894
1102	40-41	0.3889956564748758
1102	42-43	0.38814590140386684
1102	44-45	0.3568719154422766
1102	46-47	0.29105879968342663
1102	48-49	0.21869805344375237
1102	50-51	0.3374658353613924
1102	52-53	0.4217389908094944
1102	54-55	0.32563137338635073
1102	56-57	0.37933048897856025
1102	58-59	0.3135449715220062
1102	60-61	0.3462092120167881
1102	62-63	0.3520530826392658
1102	64-65	0.28212231065124627
1102	66-67	0.2544443502293561
1102	68-69	0.28085335619726237
1102	70-71	0.2647072205623573
1102	72-73	0.2480442259816229
1102	74-75	0.26825287454119007
1102	76-77	0.29711661995073513
1102	78-79	0.36196346393408163
1102	80-81	0.39309204434839273
1102	82-83	0.3845249737685563
1102	84-85	0.38633074923674826
1102	86-87	0.411029419888699
1102	88-89	0.3736327863707274
1102	90-91	0.38496734546689737
1102	92-93	0.4051648181063783
1102	94-95	0.43547855265559576
1102	96-97	0.4320588111681367
1102	98-99	0.4540844304674536
1102	100	0.5109932924586182
1103	1	0.167621584581795
1103	2	0.16019834044118753
1103	3	0.305346816442178
1103	4	0.18694056135250747
1103	5	0.1482003456758676
1103	6	0.07865402449824188
1103	7	0.16873652675486994
1103	8	0.1868215927718282
1103	9	0.23836894203456183
1103	10-11	0.21436034553680372
1103	12-13	0.2011140710821877
1103	14-15	0.28410585278102474
1103	16-17	0.2389067947730581
1103	18-19	0.27551782914057554
1103	20-21	0.29066677075821445
1103	22-23	0.27437450320309864
1103	24-25	0.3092840646728092
1103	26-27	0.29722361701808353
1103	28-29	0.34579341166892874
1103	30-31	0.2791787081223944
1103	32-33	0.3011018330234023
1103	34-35	0.31463191225657994
1103	36-37	0.36294602619643257
1103	38-39	0.32547493081212053
1103	40-41	0.3199378563786084
1103	42-43	0.3937663550814321
1103	44-45	0.3915691279444147
1103	46-47	0.2965988220731006
1103	48-49	0.21759967585231266
1103	50-51	0.2793752465280832
1103	52-53	0.2958701369063377
1103	54-55	0.24442703681960865
1103	56-57	0.24572995756093974
1103	58-59	0.35788864884090543
1103	60-61	0.3275068353897197
1103	62-63	0.2978058512121251
1103	64-65	0.344609107401709
1103	66-67	0.3160729568344749
1103	68-69	0.31366403192546244
1103	70-71	0.34635329170522056
1103	72-73	0.3411882621591644
1103	74-75	0.28297089401581843
1103	76-77	0.2423370646926557
1103	78-79	0.27084467768046494
1103	80-81	0.290592415261699
1103	82-83	0.2806821959144443
1103	84-85	0.3093870054063501
1103	86-87	0.2612113570846475
1103	88-89	0.2804521119660208
1103	90-91	0.341638959049412
1103	92-93	0.3651499731439358
1103	94-95	0.330386597765397
1103	96-97	0.38936736299610786
1103	98-99	0.4875964379430435
1103	100	0.5263755101999088
1104	1	0.20253996491857507
1104	2	0.22978054073044518
1104	3	0.31874113845398355
1104	4	0.20761773165489927
1104	5	0.16801224551341676
1104	6	0.2209731194018545
1104	7	0.22909952735250272
1104	8	0.22772106915565615
1104	9	0.23099438398248395
1104	10-11	0.22548868737573002
1104	12-13	0.25955802973707875
1104	14-15	0.33594191054886124
1104	16-17	0.35341246498718704
1104	18-19	0.33460916153561726
1104	20-21	0.3360582843863327
1104	22-23	0.32609267444971834
1104	24-25	0.3475375828455114
1104	26-27	0.3536765628247025
1104	28-29	0.40494201711369016
1104	30-31	0.41732227580622094
1104	32-33	0.44382406622289494
1104	34-35	0.39029206057940513
1104	36-37	0.4177025843554034
1104	38-39	0.4565296578531033
1104	40-41	0.41806815145324094
1104	42-43	0.4718418915329252
1104	44-45	0.506159928301372
1104	46-47	0.503962872980317
1104	48-49	0.4713658293222238
1104	50-51	0.46946378997461835
1104	52-53	0.4376620322738063
1104	54-55	0.4395811780518315
1104	56-57	0.458166530037154
1104	58-59	0.5101301325874346
1104	60-61	0.4617619091867198
1104	62-63	0.4902418811228628
1104	64-65	0.3976593781175879
1104	66-67	0.45550675736797075
1104	68-69	0.3407952615083971
1104	70-71	0.45733246852812215
1104	72-73	0.400322088768192
1104	74-75	0.3811440477140948
1104	76-77	0.3450215491015953
1104	78-79	0.36223742301319106
1104	80-81	0.4127952675365094
1104	82-83	0.38450305132155194
1104	84-85	0.39941264769859686
1104	86-87	0.3760907293930771
1104	88-89	0.38448086307747786
1104	90-91	0.3434904434923567
1104	92-93	0.43704168031406
1104	94-95	0.4908163865535684
1104	96-97	0.5185556850435411
1104	98-99	0.48859404984514754
1104	100	0.44292485045648533
1105	1	0.22304323316837937
1105	2	0.16919390620252983
1105	3	0.2751741978658906
1105	4	0.17340504785182986
1105	5	0.14593730030410512
1105	6	0.13657032824359305
1105	7	0.17077310922379496
1105	8	0.19597037731534073
1105	9	0.23935358056493072
1105	10-11	0.24956265767553276
1105	12-13	0.1949237551426819
1105	14-15	0.2588081253283363
1105	16-17	0.29058566879317027
1105	18-19	0.24636468954123103
1105	20-21	0.28770256139880956
1105	22-23	0.27630201131933774
1105	24-25	0.2759815466587128
1105	26-27	0.2966984368771364
1105	28-29	0.30420678564944836
1105	30-31	0.3156083541751258
1105	32-33	0.3510964881821579
1105	34-35	0.38588339693470175
1105	36-37	0.35732008590029096
1105	38-39	0.3534120200511808
1105	40-41	0.3597157485315847
1105	42-43	0.36389027621533643
1105	44-45	0.3505452939116367
1105	46-47	0.34140185477561147
1105	48-49	0.21513609995557204
1105	50-51	0.25813405561827096
1105	52-53	0.277744592524364
1105	54-55	0.22582322812534272
1105	56-57	0.19330973426534825
1105	58-59	0.19973915165190448
1105	60-61	0.2904682733499726
1105	62-63	0.27996318750161464
1105	64-65	0.26465226204578585
1105	66-67	0.2999970600888986
1105	68-69	0.3029268397487215
1105	70-71	0.30942439788940135
1105	72-73	0.29777488817863684
1105	74-75	0.3023134785434962
1105	76-77	0.24612398368862642
1105	78-79	0.25524981664634083
1105	80-81	0.22230217058675095
1105	82-83	0.27003863743791356
1105	84-85	0.22560741082244817
1105	86-87	0.2007635781631123
1105	88-89	0.24001145949947755
1105	90-91	0.27006678389698635
1105	92-93	0.2879047439096283
1105	94-95	0.2581352318615302
1105	96-97	0.311917050223915
1105	98-99	0.4063459430503329
1105	100	0.4229678769747913
1106	1	0.22687655167635512
1106	2	0.18952703198085885
1106	3	0.29493343406348416
1106	4	0.20121845590496434
1106	5	0.20513332117820227
1106	6	0.09319763358665512
1106	7	0.12524614189887728
1106	8	0.17782717918926494
1106	9	0.2435568658641003
1106	10-11	0.19618603429890413
1106	12-13	0.1926798631898805
1106	14-15	0.2685371548604465
1106	16-17	0.24883457171673484
1106	18-19	0.2327774349009033
1106	20-21	0.2697026132875564
1106	22-23	0.20822597206178983
1106	24-25	0.23967639632958537
1106	26-27	0.2925295093434812
1106	28-29	0.30560099931249596
1106	30-31	0.2986663435601713
1106	32-33	0.2852730507748191
1106	34-35	0.2967036098268352
1106	36-37	0.33834733490698454
1106	38-39	0.3471933015550661
1106	40-41	0.31370021155714767
1106	42-43	0.30388931256710805
1106	44-45	0.29283232765414624
1106	46-47	0.3420196644773199
1106	48-49	0.37073304296689713
1106	50-51	0.35546367519960853
1106	52-53	0.3283998362532401
1106	54-55	0.3214479686815892
1106	56-57	0.30909607121606797
1106	58-59	0.35573485971096375
1106	60-61	0.2986128428215977
1106	62-63	0.2925032900930873
1106	64-65	0.29834881663274615
1106	66-67	0.30528422725961946
1106	68-69	0.3554410350283277
1106	70-71	0.3662929859223709
1106	72-73	0.3444759126108323
1106	74-75	0.24794896748377937
1106	76-77	0.27180854456154435
1106	78-79	0.3601468916033497
1106	80-81	0.392046025476148
1106	82-83	0.3750375996629103
1106	84-85	0.34783647741795676
1106	86-87	0.3492585820177041
1106	88-89	0.39364478393759583
1106	90-91	0.34968069452973083
1106	92-93	0.4068215662425487
1106	94-95	0.40356624206137326
1106	96-97	0.4936327146964281
1106	98-99	0.4879899269203456
1106	100	0.5236024763936378
1107	1	0.1765258676960002
1107	2	0.14868485788714025
1107	3	0.2181817820227714
1107	4	0.11652081398428038
1107	5	0.12460972292470984
1107	6	0.12542264898086586
1107	7	0.12360948812959549
1107	8	0.11501154687410065
1107	9	0.12690480769551016
1107	10-11	0.1295922355190413
1107	12-13	0.09141739940557159
1107	14-15	0.19002289804305406
1107	16-17	0.14374698038452038
1107	18-19	0.16550888429089383
1107	20-21	0.18134555351260673
1107	22-23	0.15606443088270794
1107	24-25	0.15467185227187485
1107	26-27	0.229344972438696
1107	28-29	0.23187000709283012
1107	30-31	0.17227640826243373
1107	32-33	0.23717469818853232
1107	34-35	0.20541479254480066
1107	36-37	0.18091246785595416
1107	38-39	0.1928912953019264
1107	40-41	0.2043352330741257
1107	42-43	0.2750504422637974
1107	44-45	0.2332528268690126
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2216	94-95	-1.3716042298836157
2216	96-97	-1.4676257414776224
2216	98-99	-1.6284214885666302
2216	100	-1.5697943130441487
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	227.0
3	31.0
4	47.0
5	93.0
6	225.0
7	675.0
8	1542.0
9	2531.0
10	3461.0
11	4764.0
12	6587.0
13	9022.0
14	10670.0
15	13596.0
16	17422.0
17	24506.0
18	24053.0
19	13907.0
20	11517.0
21	11715.0
22	12689.0
23	14202.0
24	16830.0
25	21069.0
26	26889.0
27	35000.0
28	45795.0
29	59411.0
30	77986.0
31	100569.0
32	129857.0
33	170319.0
34	234014.0
35	343555.0
36	570146.0
37	1005498.0
38	1255602.0
39	417858.0
40	1115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.32467798388391	19.77819117848534	14.117338234482576	23.779792603148174
2	16.005107566674724	20.430437093117245	38.67806036002168	24.886394980186346
3	18.501404154850004	24.784115851028595	30.303461452035624	26.411018542085774
4	13.195908918288058	16.727990861754215	36.809192260776534	33.2669079591812
5	13.304444413678823	37.57275566853639	34.93202868160669	14.1907712361781
6	31.82940556912201	37.04327693639716	16.844086095938334	14.28323139854249
7	27.653000695421404	32.47505055915927	21.591524591612984	18.28042415380634
8	25.93540254876268	35.68838573251794	19.604624850691717	18.771586868027665
9	25.522433996202338	16.091433537202914	20.630139116229092	37.75599335036566
10-11	24.690761119021428	26.441146369697943	27.936856162786118	20.931236348494515
12-13	25.118308549720485	23.938350103810645	28.374415968567114	22.568925377901753
14-15	22.43154350308382	25.68455431950959	25.938026691862326	25.945875485544267
16-17	21.73869657767401	29.064254395213286	26.662281144555244	22.534767882557464
18-19	21.708863219696305	27.509010930262402	29.258822032197067	21.523303817844226
20-21	22.54625570927977	26.67071779523185	29.240042786051852	21.54298370943653
22-23	22.359672374517974	26.46099729605676	29.26557962255551	21.913750706869763
24-25	22.180569074694514	26.624976837011367	29.15951897661124	22.03493511168288
26-27	21.924161098055237	26.692646834397888	29.242864443950538	22.140327623596338
28-29	21.977938770113646	26.65845008661914	29.380880018939383	21.98273112432783
30-31	22.194447958825933	26.77953369056489	29.069302324986996	21.956716025622182
32-33	22.055338426641967	26.713491112375582	29.22070972134407	22.010460739638383
34-35	22.190888585492193	26.556787128407088	29.227250750267842	22.025073535832878
36-37	22.326696438108566	26.639568996024803	28.889360076583735	22.14437448928289
38-39	22.193414860828494	26.836181747719717	28.963879615687432	22.00652377576435
40-41	22.134373166261447	26.64395093914038	29.06624834797129	22.15542754662688
42-43	22.11147372124903	26.72965887762483	28.935135289411733	22.223732111714405
44-45	22.186621568876987	26.782389497724434	28.785314961237873	22.245673972160706
46-47	22.273186708258518	26.854846671148746	28.46230466281029	22.409661957782447
48-49	22.35981676921827	26.9206500128276	28.207180326706112	22.512352891248018
50-51	22.343131685741323	27.01256215956939	28.034612765611218	22.60969338907806
52-53	22.309922519157215	27.106154313757692	28.09424432589196	22.489678841193133
54-55	22.35081361996748	27.24813521351394	27.968234971244254	22.43281619527433
56-57	22.268678549878157	27.078697610698764	28.018767315069677	22.633856524353398
58-59	22.241746576298016	26.78147838835048	28.242184063824617	22.734590971526888
60-61	22.392594229484104	27.0060356923208	28.02545267692406	22.575917401271038
62-63	22.11115144992353	27.114096632885364	28.074883366814312	22.69986855037679
64-65	22.19225045844232	26.94198871580666	28.132080376581385	22.73368044916964
66-67	22.14086357409612	26.915898540091803	28.060444499728803	22.88279338608327
68-69	22.27434009167581	27.002169475539855	27.909012594161748	22.814477838622587
70-71	22.30461413778249	26.91295848910723	27.924806801317793	22.857620571792488
72-73	22.18482981134097	27.022206725671182	27.905336189308876	22.887627273678973
74-75	22.235328497501147	27.0339398571061	27.779815427292974	22.95091621809978
76-77	22.306222482454054	26.934814553226133	27.696056557682397	23.062906406637417
78-79	22.237139281491192	27.018454376110324	27.72241745393914	23.02198888845934
80-81	22.150929322262815	26.963572816559683	27.7352443825424	23.1502534786351
82-83	22.104217796760437	26.925463705243587	27.773293623809952	23.197024874186024
84-85	22.20828026556268	26.835850216241735	27.68975187020959	23.266117647985997
86-87	22.2303361776078	26.934443524066072	27.62957650690777	23.205643791418357
88-89	22.117600399923408	26.839107720954168	27.69054243765414	23.352749441468283
90-91	22.004349641667357	26.735716509266126	27.725712077833048	23.534221771233472
92-93	22.03317104299622	26.77877891608685	27.698504625679305	23.48954541523763
94-95	22.013168319140945	26.6020473378314	27.80579737896709	23.57898696406056
96-97	22.0324103795347	26.620305977566076	27.751491313680894	23.59579232921833
98-99	21.960123641546865	26.718644765277443	27.678822082523407	23.642409510652282
100	21.92371083165102	26.5806319418027	27.756135027474343	23.73952219907193
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	861.0
1	639.0
2	844.0
3	1317.0
4	869.0
5	497.0
6	1197.0
7	3058.5
8	5083.0
9	5704.5
10	4281.0
11	2652.0
12	2420.0
13	2656.0
14	2978.5
15	3399.0
16	3865.0
17	4269.5
18	4733.0
19	5275.0
20	6099.0
21	7184.5
22	8602.5
23	10912.5
24	14032.5
25	18088.0
26	23966.5
27	31519.5
28	40628.0
29	51186.5
30	61591.0
31	71329.5
32	80120.0
33	88693.0
34	98344.5
35	108061.0
36	118296.5
37	129571.0
38	140078.0
39	147641.5
40	154213.5
41	161582.5
42	166670.5
43	170333.5
44	172006.0
45	171463.5
46	170266.0
47	166883.5
48	162498.5
49	159146.5
50	157044.0
51	152487.5
52	144610.0
53	138228.5
54	133602.5
55	127767.0
56	118273.5
57	107288.5
58	98499.0
59	91718.0
60	82614.5
61	70968.5
62	61640.5
63	52688.5
64	43231.5
65	35013.0
66	27949.5
67	21933.5
68	17036.5
69	13199.0
70	9737.0
71	7012.5
72	5103.0
73	3491.0
74	2401.0
75	1650.5
76	1053.5
77	637.0
78	387.0
79	249.0
80	142.0
81	67.0
82	35.0
83	16.0
84	9.0
85	9.0
86	8.5
87	4.0
88	1.5
89	1.5
90	1.0
91	1.0
92	0.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.5
98	1.5
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03565499004791273
2	0.0
3	0.0
4	4.2598554418055825E-5
5	0.0
6	0.0
7	0.0
8	2.3429204929930703E-4
9	0.0
10-11	0.0
12-13	0.04420664984733743
14-15	1.2779566325416748E-4
16-17	2.1299277209027912E-5
18-19	5.3248193022569786E-5
20-21	1.4909494046319536E-4
22-23	0.0
24-25	1.0649638604513957E-4
26-27	7.454747023159768E-5
28-29	3.5143807394896054E-4
30-31	2.1299277209027912E-5
32-33	2.4494168790382097E-4
34-35	1.0649638604513957E-4
36-37	7.454747023159768E-5
38-39	3.088395195309047E-4
40-41	2.1299277209027914E-4
42-43	4.898833758076419E-4
44-45	2.662409651128489E-4
46-47	9.58467474406256E-5
48-49	8.839200041746583E-4
50-51	4.6858409859861405E-4
52-53	5.963797618527815E-4
54-55	4.366351827850722E-4
56-57	5.111826530166699E-4
58-59	8.519710883611165E-5
60-61	1.5974457906770933E-4
62-63	8.626207269656304E-4
64-65	4.366351827850722E-4
66-67	0.001629394706490635
68-69	0.0017358910925357747
70-71	4.7923373720312805E-4
72-73	9.265185585927142E-4
74-75	9.158689199882002E-4
76-77	7.667739795250048E-4
78-79	0.0031203441111225887
80-81	0.002598511819501405
82-83	0.001437701211609384
84-85	0.002843453507405226
86-87	0.0014057522957958422
88-89	0.0020447306120666797
90-91	0.002673059289733003
92-93	0.0024920154334562654
94-95	0.0035463296553031477
96-97	0.0030031980864729355
98-99	0.0028328038688007124
100	0.001469650127422926
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4694995.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.9889755345225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.39871273480068	40.91108994373922
2	14.173301158149748	15.587506212578317
3	4.734999939277169	7.811183874506334
4	2.116419491994301	4.655189586642446
5	1.2088085376546567	3.3235571551506915
6	0.7109350926027953	2.345615544826116
7	0.5118070970552576	1.9700623556856582
8	0.34685159456015235	1.5258411077902667
9	0.25560812592835663	1.2650066084789593
>10	1.4349866266113978	14.215246475693395
>50	0.08149246195976104	3.028245265042371
>100	0.025029048000565216	2.1867369709522166
>500	6.599097165102806E-4	0.24235948406802563
>1k	2.717271820892866E-4	0.25996944328906746
>5k	3.88181688697944E-5	0.15539679342649915
>10k+	7.76363377395888E-5	0.5169931781304683
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12504	0.26632616222168504	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11716	0.24954233178097102	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7280	0.15505873808172319	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.1299277209027912E-5	0.0
3	0.0	0.0	0.0	4.2598554418055825E-5	0.0
4	0.0	0.0	0.0	4.2598554418055825E-5	0.0
5	0.0	0.0	0.0	4.2598554418055825E-5	0.0
6	0.0	0.0	0.0	4.2598554418055825E-5	0.0
7	0.0	0.0	0.0	4.2598554418055825E-5	0.0
8	0.0	0.0	0.0	4.2598554418055825E-5	0.0
9	0.0	0.0	0.0	4.2598554418055825E-5	0.0
10-11	0.0	2.1299277209027912E-5	0.0	4.2598554418055825E-5	0.0
12-13	0.0	2.1299277209027912E-5	0.0	4.2598554418055825E-5	0.0
14-15	0.0	3.194891581354187E-5	0.0	4.2598554418055825E-5	0.0
16-17	0.0	4.2598554418055825E-5	0.0	4.2598554418055825E-5	0.0
18-19	0.0	4.2598554418055825E-5	0.0	8.519710883611165E-5	0.0
20-21	0.0	4.2598554418055825E-5	0.0	1.490949404631954E-4	0.0
22-23	0.0	4.2598554418055825E-5	0.0	4.366351827850722E-4	0.0
24-25	0.0	6.389783162708374E-5	0.0	0.0010117156674288258	0.0
26-27	0.0	7.45474702315977E-5	0.0	0.0018849860329989703	0.0
28-29	0.0	1.0649638604513956E-4	0.0	0.005953147979923301	0.0
30-31	0.0	1.0649638604513956E-4	0.0	0.0179339914100015	0.0
32-33	0.0	1.0649638604513956E-4	0.0	0.0426518026110784	0.0
34-35	0.0	1.0649638604513956E-4	0.0	0.08329082352590365	0.0
36-37	0.0	1.0649638604513956E-4	0.0	0.14692241418787455	0.0
38-39	0.0	1.2779566325416748E-4	0.0	0.2315870410937605	0.0
40-41	0.0	1.2779566325416748E-4	0.0	0.31749767571637455	0.0
42-43	0.0	1.2779566325416748E-4	0.0	0.40307817154224873	0.0
44-45	0.0	1.2779566325416748E-4	0.0	0.5079451628808976	0.0
46-47	6.389783162708374E-5	1.5974457906770933E-4	0.0	0.6333233581718405	0.0
48-49	6.389783162708374E-5	2.1299277209027912E-4	0.0	0.7616728026334426	0.0
50-51	6.389783162708374E-5	2.1299277209027912E-4	0.0	0.8875302316615885	0.0
52-53	1.0649638604513956E-4	2.1299277209027912E-4	0.0	1.010416411519075	0.0
54-55	1.0649638604513956E-4	2.1299277209027912E-4	0.0	1.146220603003837	0.0
56-57	1.0649638604513956E-4	2.3429204929930703E-4	0.0	1.3093411175091774	0.0
58-59	1.0649638604513956E-4	2.3429204929930703E-4	0.0	1.4788194662614123	0.0
60-61	1.0649638604513956E-4	2.3429204929930703E-4	0.0	1.6497887644182794	0.0
62-63	1.0649638604513956E-4	2.4494168790382097E-4	0.0	1.820853909322587	0.0
64-65	1.0649638604513956E-4	2.5559132650833496E-4	0.0	1.999427049443077	0.0
66-67	1.0649638604513956E-4	2.5559132650833496E-4	0.0	2.2046988335450837	0.0
68-69	1.0649638604513956E-4	2.7689060371736284E-4	0.0	2.4285222880961532	0.0
70-71	1.1714602464965351E-4	2.7689060371736284E-4	0.0	2.6615044318471055	0.0
72-73	1.2779566325416748E-4	3.1948915813541866E-4	0.0	2.899481256103574	0.0
74-75	1.2779566325416748E-4	3.620877125534745E-4	0.0	3.1336242104624183	0.0
76-77	1.2779566325416748E-4	3.620877125534745E-4	0.0	3.38828901841216	0.0
78-79	1.2779566325416748E-4	3.620877125534745E-4	0.0	3.6726663180684964	0.0
80-81	1.3844530185868145E-4	3.833869897625024E-4	0.0	3.976553755648302	0.0
82-83	1.490949404631954E-4	4.046862669715303E-4	0.0	4.284839493971772	0.0
84-85	1.490949404631954E-4	4.046862669715303E-4	0.0	4.586341412504167	0.0
86-87	1.490949404631954E-4	4.046862669715303E-4	0.0	4.90824803860281	0.0
88	1.490949404631954E-4	4.046862669715303E-4	0.0	5.16526641668415	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7755	0.0	56.438667	1
GTATCAA	13405	0.0	39.594604	1
TCAACGC	16815	0.0	31.107737	4
ATCAACG	16825	0.0	31.089247	3
GTGGTAT	3165	0.0	30.301584	1
TATCAAC	17360	0.0	30.185284	2
CAACGCA	17345	0.0	30.156874	5
AACGCAG	18050	0.0	29.035034	6
GTACATG	14545	0.0	25.114017	1
ACGCAGA	21140	0.0	24.546236	7
TGGTATC	3735	0.0	23.907442	2
CGCAGAG	21600	0.0	23.805689	8
TACATGG	14755	0.0	23.506462	2
ACATGGG	15105	0.0	22.183952	3
GCAGAGT	23430	0.0	21.524458	9
CAGAGTA	22420	0.0	20.112509	10-11
GAGTACT	15395	0.0	19.86794	12-13
AGAGTAC	21555	0.0	19.588274	10-11
GTACTTT	16515	0.0	18.454075	14-15
AGTACTT	15625	0.0	18.38681	12-13
>>END_MODULE