FastQCFastQC Report
Wed 25 May 2016
SRR2049369_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049369_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5255794
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59500.045.833751
GTATCAA100450.034.9659271
TCAACGC105400.032.695824
ATCAACG107450.032.0282753
CAACGCA112350.030.7150975
TATCAAC118150.029.2072872
AACGCAG120450.028.6031866
ACGCAGA136450.024.835617
GTGGTAT33300.024.709741
CGCAGAG136450.024.491158
GTACATG136550.022.450721
TACATGG137550.020.71292
GCAGAGT155650.020.2019949
TGGTATC37000.020.0763262
ACATGGG144050.018.9296953
GTCGTAT2803.92738E-818.4717921
CAGAGTA146750.017.71186410-11
GAGTACT65050.017.44966512-13
AGAGTAC131250.016.97447210-11
CATGGGG103400.016.1412324
Produced by FastQC (version 0.11.5)