##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049369_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5255794
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.860243380923986	31.0	31.0	34.0	28.0	34.0
2	31.06682872273913	31.0	31.0	34.0	28.0	34.0
3	31.116955497114233	31.0	31.0	34.0	28.0	34.0
4	34.71292215790802	37.0	35.0	37.0	32.0	37.0
5	34.743776677700836	37.0	35.0	37.0	32.0	37.0
6	34.729610407105	37.0	35.0	37.0	32.0	37.0
7	34.685144432981964	37.0	35.0	37.0	32.0	37.0
8	34.65001900759429	37.0	35.0	37.0	32.0	37.0
9	36.23856928182497	39.0	37.0	39.0	32.0	39.0
10-11	36.21082009683028	39.0	37.0	39.0	32.0	39.0
12-13	36.1634310629374	39.0	37.0	39.0	32.0	39.0
14-15	37.38758282763746	40.0	38.0	41.0	32.0	41.0
16-17	37.35781891375499	40.0	38.0	41.0	32.0	41.0
18-19	37.3879635503218	40.0	38.0	41.0	32.0	41.0
20-21	37.46690500046235	40.0	38.0	41.0	32.0	41.0
22-23	37.41199655085416	40.0	38.0	41.0	32.0	41.0
24-25	37.418406143771996	40.0	38.0	41.0	32.0	41.0
26-27	37.406475120600234	40.0	38.0	41.0	32.0	41.0
28-29	37.342644422517324	40.0	38.0	41.0	32.0	41.0
30-31	37.271390107755366	40.0	38.0	41.0	32.0	41.0
32-33	37.212363726584414	40.0	38.0	41.0	31.0	41.0
34-35	37.100043304589185	40.0	37.0	41.0	31.0	41.0
36-37	37.01585364647092	40.0	37.0	41.0	31.0	41.0
38-39	36.915353132181366	40.0	37.0	41.0	30.5	41.0
40-41	36.812255389004974	40.0	37.0	41.0	30.0	41.0
42-43	36.67566013812566	40.0	37.0	41.0	30.0	41.0
44-45	36.56036661634759	40.0	37.0	41.0	30.0	41.0
46-47	36.44902378213453	40.0	36.5	41.0	30.0	41.0
48-49	36.327877957164986	40.0	36.0	41.0	30.0	41.0
50-51	36.194815188723155	40.0	36.0	41.0	29.5	41.0
52-53	36.063657746098876	39.5	36.0	41.0	29.0	41.0
54-55	35.93488595633695	39.0	35.0	41.0	29.0	41.0
56-57	35.8157531478593	39.0	35.0	41.0	28.5	41.0
58-59	35.61736171547058	39.0	35.0	41.0	28.0	41.0
60-61	35.41138474985892	39.0	35.0	41.0	28.0	41.0
62-63	35.20627844241993	38.5	35.0	40.0	28.0	41.0
64-65	34.95901703910009	38.0	34.5	40.0	27.0	41.0
66-67	34.686717553998506	37.5	34.0	40.0	26.5	41.0
68-69	34.37742917625767	37.0	34.0	40.0	26.0	41.0
70-71	34.06681587977002	37.0	34.0	39.5	26.0	41.0
72-73	33.69207174025466	36.0	34.0	39.0	26.0	41.0
74-75	33.30056686011666	36.0	33.5	39.0	25.0	40.5
76-77	32.899454107219576	35.0	33.0	38.5	24.0	40.0
78-79	32.49177298425319	35.0	33.0	37.0	23.5	39.0
80-81	32.03634084593118	35.0	33.0	37.0	21.0	39.0
82-83	31.6207606310293	35.0	32.0	36.5	19.5	39.0
84-85	31.23153314228069	35.0	32.0	36.0	16.5	37.0
86-87	30.807147882888863	35.0	32.0	36.0	7.5	37.0
88-89	30.430227763873546	35.0	32.0	35.0	2.0	37.0
90-91	30.10408693719731	35.0	31.5	35.0	2.0	36.0
92-93	29.755883792249087	35.0	31.0	35.0	2.0	36.0
94-95	29.45468011113069	35.0	31.0	35.0	2.0	36.0
96-97	29.10614875697183	34.5	31.0	35.0	2.0	35.5
98-99	28.80545432336199	34.0	30.5	35.0	2.0	35.0
100	28.638784739280116	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3517446732141778
1101	2	0.41468184989473045
1101	3	0.3707252774240217
1101	4	0.3821525916119768
1101	5	0.40259699187525655
1101	6	0.4746486123688882
1101	7	0.4451565711095782
1101	8	0.4661192249350634
1101	9	0.5961736772927395
1101	10-11	0.5696377792926057
1101	12-13	0.5267363373599565
1101	14-15	0.6697710182045356
1101	16-17	0.6615235866348712
1101	18-19	0.6228773282037352
1101	20-21	0.596486626975171
1101	22-23	0.6367492635843419
1101	24-25	0.6551878497032604
1101	26-27	0.5522051618415205
1101	28-29	0.6013443080443324
1101	30-31	0.6377787737381411
1101	32-33	0.6437160296268445
1101	34-35	0.6950533547637363
1101	36-37	0.663768503956021
1101	38-39	0.7172620833225452
1101	40-41	0.7392362044362173
1101	42-43	0.7128793960652189
1101	44-45	0.6169524264901867
1101	46-47	0.6251282516960615
1101	48-49	0.5440817205755835
1101	50-51	0.6253179066485686
1101	52-53	0.629043770435338
1101	54-55	0.6783004168122702
1101	56-57	0.6157754009759557
1101	58-59	0.6457897547685008
1101	60-61	0.6476456480032766
1101	62-63	0.6530003062702079
1101	64-65	0.6471256828872924
1101	66-67	0.636096182011542
1101	68-69	0.5914502200252301
1101	70-71	0.7015125934847362
1101	72-73	0.7510136886887011
1101	74-75	0.7375880704234774
1101	76-77	0.7566242869018325
1101	78-79	0.7090250799386837
1101	80-81	0.7445942217916226
1101	82-83	0.7748295357771546
1101	84-85	0.7493820471746844
1101	86-87	0.7827859280030545
1101	88-89	0.813850343932522
1101	90-91	0.8651929647926018
1101	92-93	0.825265931531991
1101	94-95	0.8644490532254316
1101	96-97	0.8270956282604303
1101	98-99	0.8654253557354181
1101	100	0.9071563608157263
1102	1	0.20731725090471542
1102	2	0.2809872761999195
1102	3	0.2971318675940289
1102	4	0.3273357295371184
1102	5	0.2448672793965585
1102	6	0.36792885781993334
1102	7	0.43762839484598715
1102	8	0.43303051369379375
1102	9	0.4645579061387437
1102	10-11	0.39715072447818756
1102	12-13	0.4420032065999848
1102	14-15	0.5221756820481573
1102	16-17	0.5169047181898918
1102	18-19	0.5271683177145903
1102	20-21	0.48229744438661726
1102	22-23	0.48953769467253494
1102	24-25	0.5312686054927198
1102	26-27	0.47616141262562905
1102	28-29	0.5336989107090417
1102	30-31	0.5235584286301034
1102	32-33	0.5603874621750791
1102	34-35	0.5189130450054122
1102	36-37	0.48490784374367735
1102	38-39	0.4733546683697796
1102	40-41	0.48854710988346284
1102	42-43	0.5022302833807046
1102	44-45	0.40693621774941846
1102	46-47	0.32064950439973217
1102	48-49	0.38565586568027754
1102	50-51	0.4703847634735894
1102	52-53	0.48978564377716793
1102	54-55	0.46219095958278444
1102	56-57	0.3744195627332161
1102	58-59	0.464417814704845
1102	60-61	0.40561837813535817
1102	62-63	0.49226875184719887
1102	64-65	0.4116265233618819
1102	66-67	0.5351556469830996
1102	68-69	0.554349101235978
1102	70-71	0.5618911028113445
1102	72-73	0.5883300490479613
1102	74-75	0.621930701450097
1102	76-77	0.6816430247969052
1102	78-79	0.6351694088062843
1102	80-81	0.5713782851657072
1102	82-83	0.5552662887276192
1102	84-85	0.6331398785725497
1102	86-87	0.6426546835091393
1102	88-89	0.593701960725955
1102	90-91	0.6375968451983702
1102	92-93	0.613394932709113
1102	94-95	0.62746133293113
1102	96-97	0.5534919187418019
1102	98-99	0.6311019010521655
1102	100	0.5464968599837121
1103	1	0.1814507741614797
1103	2	0.2866519588065728
1103	3	0.2796838262601469
1103	4	0.32150352354396006
1103	5	0.36283749765686224
1103	6	0.3689523427936052
1103	7	0.42479337375550585
1103	8	0.37066345740623063
1103	9	0.45181137966241636
1103	10-11	0.5026971721165197
1103	12-13	0.5035422952434132
1103	14-15	0.5742243837309005
1103	16-17	0.5823883478603307
1103	18-19	0.5267901610031132
1103	20-21	0.5043661740295562
1103	22-23	0.5573706141993853
1103	24-25	0.532194056631802
1103	26-27	0.5146399862157267
1103	28-29	0.5111625024397952
1103	30-31	0.5021294871636499
1103	32-33	0.5483900213277835
1103	34-35	0.5192762691679889
1103	36-37	0.5838495552911525
1103	38-39	0.4967009564536511
1103	40-41	0.5527548321318889
1103	42-43	0.5275172157934236
1103	44-45	0.4418215504495464
1103	46-47	0.5022683732125515
1103	48-49	0.5209974079286113
1103	50-51	0.45801151403060913
1103	52-53	0.5153633022771871
1103	54-55	0.5072872205899444
1103	56-57	0.5100546194563549
1103	58-59	0.6416903211746359
1103	60-61	0.5392342020290286
1103	62-63	0.5129392152214294
1103	64-65	0.5306322240000156
1103	66-67	0.548976950087571
1103	68-69	0.6207683449841923
1103	70-71	0.5461037663759498
1103	72-73	0.5768328302443635
1103	74-75	0.5263685155656148
1103	76-77	0.6174733126729706
1103	78-79	0.5915152577925014
1103	80-81	0.5796207219581007
1103	82-83	0.5766822054892664
1103	84-85	0.7026563237483039
1103	86-87	0.6368273938834363
1103	88-89	0.5288286210272695
1103	90-91	0.5804077144502919
1103	92-93	0.5954054305478138
1103	94-95	0.6858132212711823
1103	96-97	0.6070961003838242
1103	98-99	0.6385172507438419
1103	100	0.6741567248437796
1104	1	0.11614571289076991
1104	2	0.1783024788814025
1104	3	0.18431421482832988
1104	4	0.23713378650433725
1104	5	0.28647748672814544
1104	6	0.28110207176202096
1104	7	0.28437080233799605
1104	8	0.2650575751728823
1104	9	0.36210059513161497
1104	10-11	0.3646802618514684
1104	12-13	0.30246946248645656
1104	14-15	0.45666772583449955
1104	16-17	0.46984799530621757
1104	18-19	0.3911058727579686
1104	20-21	0.44223246632366653
1104	22-23	0.4227015281201574
1104	24-25	0.4600816646552133
1104	26-27	0.36922248506478184
1104	28-29	0.41236109618931494
1104	30-31	0.4494687251781926
1104	32-33	0.425781130355638
1104	34-35	0.4218349643618353
1104	36-37	0.3465903233258274
1104	38-39	0.38556370025743547
1104	40-41	0.4235133403901088
1104	42-43	0.48872797066294993
1104	44-45	0.4529146211038011
1104	46-47	0.3474744995316783
1104	48-49	0.3402722748637004
1104	50-51	0.3886993616279497
1104	52-53	0.4072070731741064
1104	54-55	0.375443718454747
1104	56-57	0.3972499613282352
1104	58-59	0.47611701341961776
1104	60-61	0.4397884639076679
1104	62-63	0.4219297406046607
1104	64-65	0.48031720879756534
1104	66-67	0.4749048255804311
1104	68-69	0.47607061698163733
1104	70-71	0.49037379420741445
1104	72-73	0.5000254981459449
1104	74-75	0.5164869963182355
1104	76-77	0.5336628924605336
1104	78-79	0.43019158818393777
1104	80-81	0.46450008275637344
1104	82-83	0.3979117917828141
1104	84-85	0.41134291164689785
1104	86-87	0.4357740219821018
1104	88-89	0.4267936839523294
1104	90-91	0.43179163542487586
1104	92-93	0.4306750667948336
1104	94-95	0.45992981679258094
1104	96-97	0.4619282482412075
1104	98-99	0.5082186063334326
1104	100	0.5033544000687655
1105	1	0.12415374612956853
1105	2	0.19809339475744636
1105	3	0.17987006778282577
1105	4	0.22530154297808025
1105	5	0.1831917018840059
1105	6	0.2984307778079085
1105	7	0.22748208602050823
1105	8	0.2691009504748081
1105	9	0.3481858088631
1105	10-11	0.30334668166367607
1105	12-13	0.28310288188544064
1105	14-15	0.3042617634125264
1105	16-17	0.33824914793382277
1105	18-19	0.3124227235885897
1105	20-21	0.31091231376468187
1105	22-23	0.3352195962726512
1105	24-25	0.3048799021464106
1105	26-27	0.2525040619806518
1105	28-29	0.30572323854458006
1105	30-31	0.2966781945871517
1105	32-33	0.3238310234954014
1105	34-35	0.33972663459762487
1105	36-37	0.29849521354461217
1105	38-39	0.33650036855313203
1105	40-41	0.40022356933328496
1105	42-43	0.3826103027720791
1105	44-45	0.3997369567777156
1105	46-47	0.38346472230815465
1105	48-49	0.4043862017293023
1105	50-51	0.41694363398109857
1105	52-53	0.42990311325387864
1105	54-55	0.403261905146735
1105	56-57	0.3539361479676799
1105	58-59	0.3904527575514436
1105	60-61	0.35167826013005055
1105	62-63	0.29855761638071243
1105	64-65	0.3562778707195875
1105	66-67	0.38171589310192644
1105	68-69	0.35492130811076805
1105	70-71	0.38884044595079104
1105	72-73	0.3251675744879847
1105	74-75	0.40727449570727003
1105	76-77	0.3728225610834315
1105	78-79	0.26185802880073794
1105	80-81	0.3068715765745793
1105	82-83	0.2542060736347196
1105	84-85	0.2613702110024647
1105	86-87	0.2833456212769043
1105	88-89	0.3164375727275228
1105	90-91	0.35290826708715173
1105	92-93	0.35479066836320783
1105	94-95	0.2980035458572132
1105	96-97	0.3418626200969719
1105	98-99	0.34066263515285655
1105	100	0.3650813970442961
1106	1	0.15211480314105685
1106	2	0.11649869709105687
1106	3	0.16272434953188863
1106	4	0.2429660281259558
1106	5	0.21397216026979038
1106	6	0.2656265951702821
1106	7	0.2464073263803357
1106	8	0.26104767755482783
1106	9	0.32484372429699704
1106	10-11	0.2911707605668852
1106	12-13	0.30547604573264664
1106	14-15	0.357122539065422
1106	16-17	0.36895054304439157
1106	18-19	0.40303648224352884
1106	20-21	0.3069494548527132
1106	22-23	0.3629211252518161
1106	24-25	0.3842787083110366
1106	26-27	0.26782789505112703
1106	28-29	0.33136778898363417
1106	30-31	0.30188715754638196
1106	32-33	0.3543243945582688
1106	34-35	0.38828335752149457
1106	36-37	0.3333066780359957
1106	38-39	0.35184156775192577
1106	40-41	0.4059426000604205
1106	42-43	0.39951513627644886
1106	44-45	0.3312436534764842
1106	46-47	0.3110122951780667
1106	48-49	0.38893162468536957
1106	50-51	0.3672278866570693
1106	52-53	0.3502081832749653
1106	54-55	0.39142166761536856
1106	56-57	0.3056451621497658
1106	58-59	0.34214953833231476
1106	60-61	0.2828435220386538
1106	62-63	0.30868638260758274
1106	64-65	0.38453750143858656
1106	66-67	0.39691026738884005
1106	68-69	0.3336579298262663
1106	70-71	0.29169876608776235
1106	72-73	0.31455357678773765
1106	74-75	0.31640206045830865
1106	76-77	0.34171189725409334
1106	78-79	0.27793780252892475
1106	80-81	0.32061424856242127
1106	82-83	0.2995353232986169
1106	84-85	0.3134179112748541
1106	86-87	0.33560432563103504
1106	88-89	0.3055165913584581
1106	90-91	0.29601406756518145
1106	92-93	0.2852344407354508
1106	94-95	0.31648012642831347
1106	96-97	0.2883423894169539
1106	98-99	0.3462638942919405
1106	100	0.2829851540254289
1107	1	0.22065376311678264
1107	2	0.20510000664447858
1107	3	0.21831837391174957
1107	4	0.23586343708918633
1107	5	0.2075491646355161
1107	6	0.2674772499929716
1107	7	0.24479458683994437
1107	8	0.3006517655765606
1107	9	0.32025843094351103
1107	10-11	0.34395958545663774
1107	12-13	0.2970449385027578
1107	14-15	0.32256461173648177
1107	16-17	0.4229078711366867
1107	18-19	0.35911776379264637
1107	20-21	0.2979529948868489
1107	22-23	0.3269702463407924
1107	24-25	0.29702970024428055
1107	26-27	0.2886694623797723
1107	28-29	0.3665084404179382
1107	30-31	0.3451245270918051
1107	32-33	0.2742370433014969
1107	34-35	0.30103487940230167
1107	36-37	0.28221183832401664
1107	38-39	0.32594059883476945
1107	40-41	0.34370643893572606
1107	42-43	0.31208066994276606
1107	44-45	0.2514279393123644
1107	46-47	0.3222000259692521
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2216	3	-1.1913238729576427
2216	4	-1.4000876905058703
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2216	90-91	-3.490088410281462
2216	92-93	-3.487040330527371
2216	94-95	-3.2727548715845955
2216	96-97	-3.558357043757699
2216	98-99	-3.3609788313792635
2216	100	-3.0264789208443688
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	104727.0
3	11956.0
4	7844.0
5	6452.0
6	6139.0
7	6775.0
8	8156.0
9	9323.0
10	10369.0
11	11476.0
12	13100.0
13	14509.0
14	16456.0
15	17053.0
16	16745.0
17	17582.0
18	16649.0
19	15109.0
20	15265.0
21	16003.0
22	17744.0
23	21002.0
24	25795.0
25	32206.0
26	40403.0
27	49313.0
28	62574.0
29	80130.0
30	98976.0
31	117210.0
32	145536.0
33	187745.0
34	251310.0
35	352549.0
36	567987.0
37	996797.0
38	1358202.0
39	507371.0
40	1256.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.14072755175212	21.006424007319975	14.90987815776613	22.942970283161777
2	15.203674636805834	20.477066259879557	40.91444488901359	23.404814214301023
3	17.97208487796057	25.81167524043838	30.809995383963795	25.406244497637253
4	12.651212124038627	17.448254896589745	39.60081084286274	30.29972213650889
5	13.001746579417015	37.83881023200476	36.058936347412256	13.100506841165974
6	29.986494165079563	38.73060380314413	18.02380796016207	13.25909407161424
7	26.23114055066423	32.921035344532044	23.107525962704923	17.740298142098805
8	24.43252075605017	36.59721460811725	20.895502500472073	18.074762135360512
9	24.39127180582829	17.398673471640908	22.191273024084158	36.01878169844665
10-11	23.358332486259318	27.383742422494635	29.434879804955973	19.82304528629008
12-13	23.85153893530448	24.95219454884132	29.35867170563549	21.837594810218715
14-15	21.405879392962657	27.315157669299587	26.512051728622698	24.766911209115058
16-17	20.8108408545137	30.62042314538885	27.249558779414073	21.319177220683386
18-19	20.721831103209418	29.52294161259897	29.25056796064297	20.504659323548644
20-21	21.891698172000613	28.664214766015373	28.82522246463318	20.61886459735084
22-23	21.644184013063487	28.53603666411246	28.727464325385714	21.092314997438336
24-25	21.422530683193212	28.73019290167959	28.649990891975403	21.1972855231518
26-27	21.16746924415274	28.657997871742865	28.767579987378166	21.40695289672623
28-29	21.179556895259335	28.595376930641013	28.927636961256407	21.297429212843237
30-31	21.487999809652976	28.89499268353617	28.51405117955673	21.102956327254123
32-33	21.172234368450887	28.718944474751858	28.79205660755897	21.31676454923829
34-35	21.400692480175422	28.42824321700631	28.800893866658544	21.370170436159725
36-37	21.54836117355882	28.613209779187315	28.447413256374666	21.3910157908792
38-39	21.322538794519527	28.617436910249637	28.69796102027788	21.36206327495296
40-41	21.24628424363218	28.315476828355802	28.960020298303597	21.478218629708426
42-43	21.03645833432471	28.287182165663776	29.009557371735145	21.666802128276373
44-45	21.078086238752487	28.380773079326506	28.91666033781416	21.624480344106846
46-47	21.09435395555063	28.52808423199659	28.780150579331	21.59741123312178
48-49	20.968597695693024	28.386746242531913	28.777751140823526	21.866904920951534
50-51	20.92266355722778	28.319010994386602	28.74374176886644	22.014583679519177
52-53	20.862379340127006	28.2723052727502	28.868508397713022	21.99680698940977
54-55	21.021970070514858	28.51531454263178	28.631251539981562	21.8314638468718
56-57	20.986074398353576	28.29780278607202	28.55627223087996	22.15985058469445
58-59	20.94584222340931	27.934296092991833	28.74075791365465	22.3791037699442
60-61	21.0958004562425	28.174849180162493	28.445292298790303	22.284058064804707
62-63	21.027266330133664	28.129919682422276	28.43905013714916	22.403763850294904
64-65	21.09870921804122	27.957676390721108	28.449040311978784	22.49457407925889
66-67	21.03276859278011	28.136498925795422	28.22916364311189	22.601568838312584
68-69	21.375144454044843	28.320701445906117	28.09735976596898	22.206794334080058
70-71	21.336513532501204	28.287013165240353	28.07094881969272	22.305524482565723
72-73	21.3158742973007	28.33463061458775	28.1491791916404	22.20031589647115
74-75	21.566155904692025	28.34844111486387	27.952222295183688	22.13318068526042
76-77	21.718090857466592	28.393165300260293	27.722345057682755	22.166398784590356
78-79	21.83617104518466	28.397329083244934	27.825624686955848	21.940875184614566
80-81	21.855875294237222	28.28308023904044	27.868859323629824	21.992185143092506
82-83	21.833593487845	28.32089089898246	27.928651110435638	21.916864502736903
84-85	22.009556036218758	28.15891083342279	27.797861454795452	22.033671675562996
86-87	22.272846579425064	28.169268224625842	27.658765333311507	21.899119862637587
88-89	22.298080708806605	27.997958044942234	27.693377835822663	22.010583410428495
90-91	22.453263785794903	27.880996279147052	27.664151932084557	22.001588002973484
92-93	22.711584742388286	27.941076264456942	27.430044028446808	21.91729496470796
94-95	22.712098394529814	27.682055309426268	27.489061225919283	22.116785070124642
96-97	22.845368368699383	27.63761288969849	27.41097729477221	22.10604144682992
98-99	23.011803735077898	27.621097782751757	27.26692674789004	22.100171734280302
100	23.091125717636572	27.553439118808694	27.276126119098276	22.079309044456462
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1071.0
1	876.0
2	844.0
3	1070.5
4	1079.5
5	908.5
6	749.0
7	652.5
8	633.0
9	661.0
10	624.5
11	601.0
12	645.0
13	731.0
14	812.0
15	965.5
16	1191.5
17	1490.0
18	1974.5
19	2709.0
20	3947.0
21	5754.5
22	8278.0
23	11864.5
24	17150.0
25	24300.5
26	33938.5
27	46178.0
28	60845.0
29	77201.0
30	93428.5
31	108521.5
32	122197.5
33	135054.5
34	147649.5
35	158933.5
36	168645.0
37	179145.5
38	188418.0
39	193739.5
40	198065.5
41	201392.0
42	200781.0
43	198105.5
44	195442.0
45	191352.5
46	186970.5
47	181610.5
48	174515.5
49	168524.0
50	162649.0
51	154988.0
52	145699.0
53	137086.5
54	129019.0
55	120764.0
56	109817.0
57	96598.5
58	85479.0
59	76225.0
60	66019.5
61	54740.0
62	45368.0
63	37264.0
64	29576.5
65	23485.5
66	18387.5
67	14172.5
68	11009.5
69	8576.5
70	6803.0
71	5260.0
72	3900.0
73	2682.0
74	1962.0
75	1492.0
76	1073.0
77	798.0
78	592.5
79	437.5
80	343.0
81	265.5
82	195.5
83	154.0
84	137.0
85	102.5
86	82.0
87	74.0
88	62.5
89	42.5
90	40.0
91	42.0
92	37.0
93	43.0
94	40.5
95	39.0
96	40.5
97	34.5
98	39.5
99	71.0
100	165.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.021500081624203686
2	0.04315237621565838
3	0.04507406492720225
4	0.047471419161405494
5	0.050782051199114726
6	0.047737791854094734
7	0.045530703828955243
8	0.045397517482610616
9	0.04532141099898512
10-11	0.045968316109801864
12-13	0.047005266949199306
14-15	0.045235791204906436
16-17	0.041592193301335637
18-19	0.04469353250907475
20-21	0.044113220571430314
22-23	0.04515968472128093
24-25	0.041573166680429256
26-27	0.04184905268357169
28-29	0.04239131137940338
30-31	0.04245790455257569
32-33	0.04223909841215238
34-35	0.04212493868671413
36-37	0.03928997217166426
38-39	0.039727584452510886
40-41	0.04096431481142526
42-43	0.04096431481142526
44-45	0.03951829162254076
46-47	0.03840523429951783
48-49	0.0385003674040497
50-51	0.04020324997517026
52-53	0.040555242461938194
54-55	0.039546831553900326
56-57	0.03770124932598195
58-59	0.03886187320127083
60-61	0.03822448140090726
62-63	0.038528907335409264
64-65	0.035142168814074524
66-67	0.03627425275800383
68-69	0.037301690286948075
70-71	0.03871917354447301
72-73	0.03534194833359146
74-75	0.03575102068307852
76-77	0.03667381179703771
78-79	0.03695921111063333
80-81	0.037197043871963015
82-83	0.03548464799038927
84-85	0.03694969780018014
86-87	0.037197043871963015
88-89	0.03815788822773495
90-91	0.03853842064586245
92-93	0.03649305889842715
94-95	0.03816740153818814
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5255794.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.91653149862543
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.65071756348932	51.84382501663237
2	13.007699764962553	17.148449025636335
3	3.919091372861395	7.749987297756279
4	1.642629681614636	4.331058045949129
5	0.8383242683437726	2.7629714020172202
6	0.474246012199133	1.8756391320732992
7	0.3241389698591961	1.495628163165899
8	0.21421058372784424	1.1296014951708309
9	0.15820246242975078	0.9385341838119697
>10	0.7281138313490482	8.198399277773312
>50	0.033408177304752366	1.4985148900198206
>100	0.00907287550651054	0.9375355273413577
>500	8.666216767169552E-5	0.04015856896385374
>1k	5.7774184413854046E-5	0.04969797368827869
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.9026620906374947E-5	0.0
2	0.0	0.0	0.0	1.9026620906374947E-5	0.0
3	0.0	0.0	0.0	1.9026620906374947E-5	0.0
4	0.0	0.0	0.0	3.8053241812749894E-5	0.0
5	0.0	0.0	0.0	5.707986271912484E-5	0.0
6	0.0	0.0	0.0	5.707986271912484E-5	0.0
7	0.0	0.0	0.0	7.610648362549979E-5	0.0
8	0.0	0.0	0.0	9.513310453187473E-5	0.0
9	0.0	0.0	0.0	1.1415972543824968E-4	0.0
10-11	0.0	0.0	0.0	1.6172627770418704E-4	0.0
12-13	0.0	0.0	0.0	1.9026620906374946E-4	0.0
14-15	0.0	0.0	0.0	2.568593822360618E-4	0.0
16-17	0.0	0.0	0.0	3.519924867679365E-4	0.0
18-19	0.0	0.0	0.0	5.137187644721235E-4	0.0
20-21	0.0	0.0	0.0	8.371713198804976E-4	0.0
22-23	0.0	0.0	0.0	0.002092928299701244	0.0
24-25	0.0	0.0	0.0	0.005004001298376611	0.0
26-27	0.0	0.0	0.0	0.009998489286300035	0.0
28-29	0.0	0.0	0.0	0.02902511019267498	0.0
30-31	0.0	0.0	0.0	0.083222439844484	0.0
32-33	0.0	0.0	0.0	0.20628662386691715	0.0
34-35	0.0	0.0	0.0	0.3996827120697653	0.0
36-37	0.0	0.0	0.0	0.6980581811235371	0.0
38-39	0.0	0.0	0.0	1.083404334340349	0.0
40-41	0.0	0.0	0.0	1.4652305626894813	0.0
42-43	9.513310453187474E-6	0.0	0.0	1.8430041207855559	0.0
44-45	1.9026620906374947E-5	0.0	0.0	2.287846517576602	0.0
46-47	1.9026620906374947E-5	0.0	0.0	2.7971225660670873	0.0
48-49	1.9026620906374947E-5	0.0	0.0	3.304714758607358	0.0
50-51	1.9026620906374947E-5	0.0	0.0	3.7922814326436693	0.0
52-53	2.8539931359562422E-5	0.0	0.0	4.249786045647908	0.0
54-55	3.8053241812749894E-5	0.0	0.0	4.738617989974493	0.0
56-57	3.8053241812749894E-5	0.0	0.0	5.290475616053445	0.0
58-59	3.8053241812749894E-5	0.0	0.0	5.8667539100657295	0.0
60-61	3.8053241812749894E-5	0.0	0.0	6.4257274923636665	0.0
62-63	3.8053241812749894E-5	0.0	0.0	6.952241659395327	0.0
64-65	3.8053241812749894E-5	0.0	0.0	7.491341936156554	0.0
66-67	3.8053241812749894E-5	0.0	0.0	8.078417837533205	0.0
68-69	3.8053241812749894E-5	0.0	0.0	8.69334871191679	0.0
70-71	3.8053241812749894E-5	0.0	0.0	9.314929390307155	0.0
72-73	3.8053241812749894E-5	0.0	0.0	9.918482345388728	0.0
74-75	3.8053241812749894E-5	0.0	0.0	10.502789873423502	0.0
76-77	3.8053241812749894E-5	0.0	0.0	11.113515864586777	0.0
78-79	3.8053241812749894E-5	0.0	0.0	11.763788687303954	0.0
80-81	3.8053241812749894E-5	0.0	0.0	12.425154030009548	0.0
82-83	3.8053241812749894E-5	0.0	0.0	13.076577963291559	0.0
84-85	3.8053241812749894E-5	0.0	0.0	13.698881653276366	0.0
86-87	3.8053241812749894E-5	0.0	0.0	14.331250425720643	0.0
88	3.8053241812749894E-5	0.0	0.0	14.826532394534489	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5950	0.0	45.83375	1
GTATCAA	10045	0.0	34.965927	1
TCAACGC	10540	0.0	32.69582	4
ATCAACG	10745	0.0	32.028275	3
CAACGCA	11235	0.0	30.715097	5
TATCAAC	11815	0.0	29.207287	2
AACGCAG	12045	0.0	28.603186	6
ACGCAGA	13645	0.0	24.83561	7
GTGGTAT	3330	0.0	24.70974	1
CGCAGAG	13645	0.0	24.49115	8
GTACATG	13655	0.0	22.45072	1
TACATGG	13755	0.0	20.7129	2
GCAGAGT	15565	0.0	20.201994	9
TGGTATC	3700	0.0	20.076326	2
ACATGGG	14405	0.0	18.929695	3
GTCGTAT	280	3.92738E-8	18.471792	1
CAGAGTA	14675	0.0	17.711864	10-11
GAGTACT	6505	0.0	17.449665	12-13
AGAGTAC	13125	0.0	16.974472	10-11
CATGGGG	10340	0.0	16.141232	4
>>END_MODULE