FastQCFastQC Report
Wed 25 May 2016
SRR2049369_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049369_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5255794
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT199700.37996161950030766No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104190.19823836322352054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104120.19810517687717596No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT101140.1924352438470762TruSeq Adapter, Index 11 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84250.058.5407941
GTATCAA151900.039.4334451
ATCAACG185850.032.064453
TCAACGC185850.032.064454
CAACGCA192850.030.9249555
TATCAAC193900.030.7090152
AACGCAG201500.029.7380716
GTGGTAT36650.028.3511941
TGGTATC37100.027.2352662
ACGCAGA228250.025.9852497
CGCAGAG230050.025.5981648
GCAGAGT249800.023.216099
GAGTACT169500.021.46910912-13
GTACATG132650.021.2311061
CAGAGTA243650.020.83889410-11
TACATGG132950.020.2904012
AGAGTAC229950.020.11682110-11
GTACTTT187850.019.10111414-15
ACATGGG137150.018.9494423
AGTACTT180700.018.38216812-13
Produced by FastQC (version 0.11.5)