##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049369_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5255794
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.819247291655646	31.0	31.0	34.0	30.0	34.0
2	31.998950301324594	33.0	31.0	34.0	30.0	34.0
3	31.922527024461004	33.0	31.0	34.0	30.0	34.0
4	35.66504984784411	37.0	35.0	37.0	33.0	37.0
5	35.7608003662244	37.0	35.0	37.0	35.0	37.0
6	35.79981102760116	37.0	35.0	37.0	35.0	37.0
7	35.766639255648144	37.0	35.0	37.0	33.0	37.0
8	35.76467057118297	37.0	35.0	37.0	33.0	37.0
9	37.50554968478597	39.0	37.0	39.0	35.0	39.0
10-11	37.466617983885975	39.0	37.0	39.0	34.5	39.0
12-13	37.412146575760005	39.0	37.0	39.0	34.0	39.0
14-15	38.69270818072398	40.0	38.0	41.0	34.0	41.0
16-17	38.713206130225046	40.0	38.0	41.0	34.0	41.0
18-19	38.78572590554349	40.0	38.0	41.0	35.0	41.0
20-21	38.82255335730434	40.0	38.0	41.0	35.0	41.0
22-23	38.79165735947794	40.0	38.0	41.0	35.0	41.0
24-25	38.76784012082665	40.0	38.0	41.0	34.0	41.0
26-27	38.64635324367735	40.0	38.0	41.0	34.0	41.0
28-29	38.56294672127561	40.0	38.0	41.0	34.0	41.0
30-31	38.46392609756014	40.0	38.0	41.0	34.0	41.0
32-33	38.33164732103275	40.0	38.0	41.0	34.0	41.0
34-35	38.228290625545824	40.0	38.0	41.0	34.0	41.0
36-37	38.1060872058532	40.0	38.0	41.0	33.5	41.0
38-39	37.995992898504014	40.0	38.0	41.0	33.0	41.0
40-41	37.90873415130045	40.0	37.5	41.0	33.0	41.0
42-43	37.78711361213929	40.0	37.0	41.0	33.0	41.0
44-45	37.62388470704902	40.0	37.0	41.0	33.0	41.0
46-47	37.41390653819385	40.0	37.0	41.0	32.0	41.0
48-49	37.21820708726408	40.0	37.0	41.0	32.0	41.0
50-51	37.07028700135508	40.0	37.0	41.0	31.0	41.0
52-53	36.94499508542382	40.0	36.5	41.0	31.0	41.0
54-55	36.79198737621756	40.0	36.0	41.0	31.0	41.0
56-57	36.65004983072015	39.0	36.0	41.0	31.0	41.0
58-59	36.44843148342572	39.0	35.0	41.0	30.5	41.0
60-61	36.24257733465201	39.0	35.0	41.0	30.0	41.0
62-63	36.024873977176426	39.0	35.0	41.0	30.0	41.0
64-65	35.76605304545802	38.5	35.0	40.5	29.5	41.0
66-67	35.442184377850424	38.0	35.0	40.0	29.0	41.0
68-69	35.09133662772932	37.0	35.0	40.0	29.0	41.0
70-71	34.71702458277474	37.0	34.0	39.5	28.0	41.0
72-73	34.406096395711096	36.0	34.0	39.0	28.0	41.0
74-75	34.086995418770215	36.0	34.0	39.0	28.0	41.0
76-77	33.747482397521665	35.0	34.0	38.5	28.0	40.0
78-79	33.397808019111864	35.0	34.0	37.0	27.0	39.0
80-81	33.058487927799305	35.0	33.5	37.0	26.5	39.0
82-83	32.74185203986305	35.0	33.0	36.5	26.0	39.0
84-85	32.46403759355865	35.0	33.0	36.0	26.0	37.0
86-87	32.221643294999765	35.0	33.0	36.0	26.0	37.0
88-89	31.982859678290282	35.0	33.0	35.0	26.0	37.0
90-91	31.77708097006846	35.0	33.0	35.0	25.0	36.0
92-93	31.584626699600477	35.0	33.0	35.0	25.0	36.0
94-95	31.420613992861973	35.0	33.0	35.0	25.0	36.0
96-97	31.21406879721694	35.0	33.0	35.0	24.0	36.0
98-99	31.053762285964787	35.0	33.0	35.0	23.5	35.0
100	30.928901322997056	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.20917639398846433
1101	2	0.21793263606058844
1101	3	0.3678907051518969
1101	4	0.22834761066037856
1101	5	0.16282663472729553
1101	6	0.13679974779532245
1101	7	0.056736816393090805
1101	8	0.10821646083534375
1101	9	0.22290853215285722
1101	10-11	0.2267006422792619
1101	12-13	0.17679610811777025
1101	14-15	0.3139838043351233
1101	16-17	0.24618869350349115
1101	18-19	0.2937487901428355
1101	20-21	0.3166282022325575
1101	22-23	0.3073103316677219
1101	24-25	0.2926164258774051
1101	26-27	0.34765519566484926
1101	28-29	0.36882812814830146
1101	30-31	0.3835314991431744
1101	32-33	0.35365748187116
1101	34-35	0.36742936656875
1101	36-37	0.37802576949706435
1101	38-39	0.35843088090021524
1101	40-41	0.3139656809756275
1101	42-43	0.35508445217325857
1101	44-45	0.36014078359688284
1101	46-47	0.39534678361932407
1101	48-49	0.3654611374586949
1101	50-51	0.27483140432168796
1101	52-53	0.3386999209429007
1101	54-55	0.34452281029603427
1101	56-57	0.32208413690636206
1101	58-59	0.3882167526959961
1101	60-61	0.3904567302872266
1101	62-63	0.3609774061870681
1101	64-65	0.2693694269119078
1101	66-67	0.31649805258457064
1101	68-69	0.3954467667780861
1101	70-71	0.3540731294015771
1101	72-73	0.33145173024822583
1101	74-75	0.40386086503134777
1101	76-77	0.2989007785849864
1101	78-79	0.33085747782965313
1101	80-81	0.3526622497692955
1101	82-83	0.34727965838489183
1101	84-85	0.39827535752525733
1101	86-87	0.2808283831522189
1101	88-89	0.3760460061158213
1101	90-91	0.38637525318033994
1101	92-93	0.4268799386821449
1101	94-95	0.4211260895201683
1101	96-97	0.42340650984844075
1101	98-99	0.4865454178938293
1101	100	0.5431368240340255
1102	1	0.17990210589579192
1102	2	0.16987168513395545
1102	3	0.3502294698338524
1102	4	0.18644426204622278
1102	5	0.1301604491742907
1102	6	0.14860002567922947
1102	7	0.15866019670936282
1102	8	0.19300080158565436
1102	9	0.1340274690957628
1102	10-11	0.2046100216341884
1102	12-13	0.19063950692886067
1102	14-15	0.2539144279708836
1102	16-17	0.20881045809168342
1102	18-19	0.24654260183760357
1102	20-21	0.17963724742065068
1102	22-23	0.22878562860682194
1102	24-25	0.1772240296629235
1102	26-27	0.2593147316496456
1102	28-29	0.2780720160061563
1102	30-31	0.13641484466972997
1102	32-33	0.1635958525804142
1102	34-35	0.27612291529455035
1102	36-37	0.3473210054180029
1102	38-39	0.28257606828032067
1102	40-41	0.3006902457637821
1102	42-43	0.3724484657073148
1102	44-45	0.34039882271924427
1102	46-47	0.26993331534955445
1102	48-49	0.22760334346739342
1102	50-51	0.35826989152742783
1102	52-53	0.3763935122058797
1102	54-55	0.22939768484790335
1102	56-57	0.3085859295829394
1102	58-59	0.30093396824479157
1102	60-61	0.2437493576716463
1102	62-63	0.30815964713931265
1102	64-65	0.24948066277993064
1102	66-67	0.24125369266406693
1102	68-69	0.3110637408187955
1102	70-71	0.31523332278957383
1102	72-73	0.3812904543544846
1102	74-75	0.35592797754095074
1102	76-77	0.36208867605854067
1102	78-79	0.39263395643303056
1102	80-81	0.32399245301777313
1102	82-83	0.3477925755713187
1102	84-85	0.2517716518295856
1102	86-87	0.2847136599280802
1102	88-89	0.25299907207313055
1102	90-91	0.2846503860936238
1102	92-93	0.374744783998743
1102	94-95	0.35079609065013884
1102	96-97	0.3782758741822754
1102	98-99	0.3883677801101122
1102	100	0.44246265595310774
1103	1	0.18763723960777412
1103	2	0.1759668800965386
1103	3	0.33554655498856434
1103	4	0.21078094134673364
1103	5	0.18915517090349
1103	6	0.16714456371239095
1103	7	0.1708690046678285
1103	8	0.14423146375137463
1103	9	0.2205142241083351
1103	10-11	0.22423256495471833
1103	12-13	0.264756057711125
1103	14-15	0.33853883364076154
1103	16-17	0.26925092205588186
1103	18-19	0.2737466607318737
1103	20-21	0.2776119755308599
1103	22-23	0.2657054568891155
1103	24-25	0.30818828048043656
1103	26-27	0.3207520808382398
1103	28-29	0.3956745340081298
1103	30-31	0.3637200119626982
1103	32-33	0.3551158818614226
1103	34-35	0.3757444063656905
1103	36-37	0.38366308714594055
1103	38-39	0.3560057951915212
1103	40-41	0.3670452832206337
1103	42-43	0.42189886719108927
1103	44-45	0.4481155897629634
1103	46-47	0.4180563856753352
1103	48-49	0.39058675813848254
1103	50-51	0.4024848386346491
1103	52-53	0.350896606875736
1103	54-55	0.33185566311433234
1103	56-57	0.3106844113206151
1103	58-59	0.3921248063665672
1103	60-61	0.36264744856544695
1103	62-63	0.3902451159127551
1103	64-65	0.4422911651387551
1103	66-67	0.4018900188373067
1103	68-69	0.37087124081806166
1103	70-71	0.41909187875119613
1103	72-73	0.3882535716191029
1103	74-75	0.3871240534285292
1103	76-77	0.3266539209508821
1103	78-79	0.3584244041218554
1103	80-81	0.42468739990192006
1103	82-83	0.39234843974104194
1103	84-85	0.36126220022322286
1103	86-87	0.37768818551329986
1103	88-89	0.4853834339741354
1103	90-91	0.47917108722926827
1103	92-93	0.46786452587037886
1103	94-95	0.42184181961828315
1103	96-97	0.530008255315515
1103	98-99	0.603514660623599
1103	100	0.625553096274718
1104	1	0.21841052697589447
1104	2	0.16173587702486003
1104	3	0.26961442943980174
1104	4	0.1596092655463437
1104	5	0.19642155499435177
1104	6	0.1715808032651509
1104	7	0.18912809152966759
1104	8	0.1494450234906992
1104	9	0.17328624370092172
1104	10-11	0.2061657541315043
1104	12-13	0.20944917638043137
1104	14-15	0.2637233322998753
1104	16-17	0.23239658729129076
1104	18-19	0.2543157797030773
1104	20-21	0.24363001431665765
1104	22-23	0.2698874868394725
1104	24-25	0.2659744121525094
1104	26-27	0.26678030471870784
1104	28-29	0.3105148045968775
1104	30-31	0.3807348921715601
1104	32-33	0.39042372265755176
1104	34-35	0.34519953165182926
1104	36-37	0.3549837739170911
1104	38-39	0.3340315648457306
1104	40-41	0.3296657142364552
1104	42-43	0.38247395879984936
1104	44-45	0.33743164269203163
1104	46-47	0.4185314533601243
1104	48-49	0.4535810255761703
1104	50-51	0.42343324863747966
1104	52-53	0.4089235170266079
1104	54-55	0.3931090369095287
1104	56-57	0.41853960640124654
1104	58-59	0.48027179452238755
1104	60-61	0.46999474664671226
1104	62-63	0.4656575328716528
1104	64-65	0.3923846886495639
1104	66-67	0.4279452846036662
1104	68-69	0.4125910679973046
1104	70-71	0.4938776491963708
1104	72-73	0.46839953708781223
1104	74-75	0.4582813316771208
1104	76-77	0.41302241667339246
1104	78-79	0.4680020067728208
1104	80-81	0.5080929020254175
1104	82-83	0.4867264526922952
1104	84-85	0.5142277282592502
1104	86-87	0.5381125477472892
1104	88-89	0.5283432553492418
1104	90-91	0.3992686435585213
1104	92-93	0.5253949231002046
1104	94-95	0.5400238359216765
1104	96-97	0.5516789102391044
1104	98-99	0.532343422083219
1104	100	0.5142326156006369
1105	1	0.10701608669569751
1105	2	0.12070745948928163
1105	3	0.23581233936539192
1105	4	0.11924197718920482
1105	5	0.14782008350974252
1105	6	0.09652649628502985
1105	7	0.14262735013278416
1105	8	0.07549662714113481
1105	9	0.14231407790781248
1105	10-11	0.1848905191479986
1105	12-13	0.13460419057656026
1105	14-15	0.1822976781338994
1105	16-17	0.18691337869547198
1105	18-19	0.14497487576633716
1105	20-21	0.1842651517613092
1105	22-23	0.15387862028351407
1105	24-25	0.19277985710033363
1105	26-27	0.19453357542355576
1105	28-29	0.2221008421434263
1105	30-31	0.18346199043610767
1105	32-33	0.23250402723941477
1105	34-35	0.2258856776823066
1105	36-37	0.17639242666589183
1105	38-39	0.17423375511557282
1105	40-41	0.17876532561072622
1105	42-43	0.2004759683764945
1105	44-45	0.13489378784125705
1105	46-47	0.11457246667432486
1105	48-49	0.20102498671400326
1105	50-51	0.1612714490778373
1105	52-53	0.21504178838392818
1105	54-55	0.21101202464195268
1105	56-57	0.20253458902774213
1105	58-59	0.24463000360998421
1105	60-61	0.2412982045228489
1105	62-63	0.2616473032149784
1105	64-65	0.23582899413001712
1105	66-67	0.2127965811330128
1105	68-69	0.21842916088059638
1105	70-71	0.20763052808144522
1105	72-73	0.2706709598303334
1105	74-75	0.27170924887565917
1105	76-77	0.24324098789020354
1105	78-79	0.25604657440444356
1105	80-81	0.23640639298152166
1105	82-83	0.21799810455202362
1105	84-85	0.24361452265275574
1105	86-87	0.20904891464315511
1105	88-89	0.2385650969541686
1105	90-91	0.23487902920958703
1105	92-93	0.2684113992015007
1105	94-95	0.29726904254759035
1105	96-97	0.3461230062733591
1105	98-99	0.3558988817509565
1105	100	0.3138451934686479
1106	1	0.0916203311863022
1106	2	0.1301564807537332
1106	3	0.22809906342939357
1106	4	0.16539012575027812
1106	5	0.12528071248070916
1106	6	0.12625142821856628
1106	7	0.08995466201950109
1106	8	0.12687937016720952
1106	9	0.16737274240821165
1106	10-11	0.13723877874626567
1106	12-13	0.1690221540225778
1106	14-15	0.2006391370534999
1106	16-17	0.18120483833076406
1106	18-19	0.17331219621387106
1106	20-21	0.13471445494822376
1106	22-23	0.18242439049789283
1106	24-25	0.16505236728550443
1106	26-27	0.20372865923167183
1106	28-29	0.2314686072774208
1106	30-31	0.24629060244500778
1106	32-33	0.18642175269236105
1106	34-35	0.20239010128179302
1106	36-37	0.1841914006698957
1106	38-39	0.19941250454580484
1106	40-41	0.2628775415799467
1106	42-43	0.3050026224194937
1106	44-45	0.26854678859657355
1106	46-47	0.3348098666285182
1106	48-49	0.30752211798542817
1106	50-51	0.35203438454213654
1106	52-53	0.3383632681432118
1106	54-55	0.2865933830976317
1106	56-57	0.29060535049038094
1106	58-59	0.32843386236368843
1106	60-61	0.2777709054591213
1106	62-63	0.21827712471930028
1106	64-65	0.1723512246779535
1106	66-67	0.18067483432412246
1106	68-69	0.18708513066552257
1106	70-71	0.21409523401209185
1106	72-73	0.24941681081568845
1106	74-75	0.21248887975981035
1106	76-77	0.2244874007043407
1106	78-79	0.3026334707272582
1106	80-81	0.29581632760535825
1106	82-83	0.3497570326175605
1106	84-85	0.32671496485286156
1106	86-87	0.31444863833697667
1106	88-89	0.35690193561612915
1106	90-91	0.28987681860267145
1106	92-93	0.2532239970697141
1106	94-95	0.31052378416329773
1106	96-97	0.38955146813031405
1106	98-99	0.39419311444046556
1106	100	0.32975613340817134
1107	1	0.12874703904558515
1107	2	0.13225329621933213
1107	3	0.228289595164906
1107	4	0.14549993119701554
1107	5	0.1196769846051069
1107	6	0.11865550276092307
1107	7	0.12101029059957824
1107	8	0.14687907682564827
1107	9	0.17608547000763508
1107	10-11	0.16440207496462023
1107	12-13	0.15543112662087566
1107	14-15	0.25987085399117404
1107	16-17	0.19310761645773056
1107	18-19	0.19135979426472716
1107	20-21	0.18885774008163736
1107	22-23	0.1753599089255644
1107	24-25	0.23404849276093387
1107	26-27	0.27955587893145406
1107	28-29	0.2764530736570876
1107	30-31	0.28443876085151487
1107	32-33	0.2841575189983345
1107	34-35	0.2829178623432682
1107	36-37	0.36227519008674847
1107	38-39	0.2904143128983492
1107	40-41	0.2721848859315017
1107	42-43	0.29774862224670073
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2216	74-75	-0.6504342333177746
2216	76-77	-0.6209406208107779
2216	78-79	-0.8250153566670804
2216	80-81	-1.045818529526862
2216	82-83	-0.9563711762914835
2216	84-85	-1.0709538861183745
2216	86-87	-0.9313648102277199
2216	88-89	-1.2244047139480223
2216	90-91	-0.9735438486788581
2216	92-93	-1.0682743455984074
2216	94-95	-1.0169802254837954
2216	96-97	-1.1042287579925087
2216	98-99	-1.267498841374067
2216	100	-1.3080917627683455
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	283.0
3	29.0
4	41.0
5	91.0
6	215.0
7	588.0
8	1609.0
9	2870.0
10	4168.0
11	6095.0
12	8321.0
13	11341.0
14	14182.0
15	18032.0
16	22259.0
17	30157.0
18	30573.0
19	18763.0
20	15854.0
21	16903.0
22	19116.0
23	22133.0
24	25385.0
25	31236.0
26	36903.0
27	38929.0
28	49060.0
29	64232.0
30	83068.0
31	108217.0
32	139028.0
33	180162.0
34	244432.0
35	347570.0
36	558201.0
37	1019577.0
38	1479284.0
39	604758.0
40	2129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.17811001089347	21.227796445375528	15.256813080993686	23.337280462737315
2	15.015314527167542	20.67478291576877	40.49831481218632	23.811587744877368
3	17.252007974437355	25.812027640352724	31.659383910404404	25.27658047480552
4	12.22075315428378	18.124966584465245	38.808749742426876	30.8455305188241
5	12.346735811943924	37.928198860153195	36.24183139597937	13.483233931923511
6	29.579450792782215	39.08809972384763	17.508030185353533	13.824419298016627
7	25.55728021303727	33.501674532905966	22.65440768797255	18.28663756608421
8	24.311751174432477	36.6300590022699	20.62162630119888	18.436563522098737
9	24.066049772879225	16.814985518838828	21.920817292306356	37.19814741597558
10-11	23.17719263730656	27.360889715236176	29.134532289507543	20.32738535794972
12-13	24.011182321173944	24.76236187465392	29.298667490502943	21.92778831366919
14-15	21.382993114830143	26.981305756376443	26.759388552318214	24.876312576475197
16-17	20.438272504592074	30.480836958221722	27.51270312344814	21.56818741373806
18-19	20.293166119698622	28.612246128865653	30.38200811428688	20.71257963714885
20-21	21.323591947850264	27.685719499013373	30.229286221345642	20.76140233179072
22-23	20.994182800924087	27.866122606784057	30.20015624661088	20.939538345680976
24-25	21.042271574993634	28.02770164216052	30.117321098951926	20.812705683893924
26-27	20.81012361508702	27.969931449893217	30.23589886240709	20.984046072612674
28-29	20.53853754282673	27.67088786783138	30.594873711202407	21.19570087813948
30-31	21.062113848672677	27.868489861424322	30.368017763258347	20.70137852664465
32-33	20.64716338623221	27.989866399532325	30.41736411276532	20.945606101470144
34-35	20.764703089915194	27.782057183965534	30.626233936410905	20.827005789708362
36-37	21.192465832919506	27.817283415052735	29.98583466995447	21.004416082073295
38-39	20.90316444232022	27.684142170442577	30.239825669891562	21.17286771734564
40-41	20.762677116604188	27.39813185042246	30.508711441802554	21.330479591170803
42-43	20.86313037808296	27.350492968244676	30.485802779024823	21.30057387464754
44-45	20.743769999417783	27.625729245032797	30.210693830675496	21.419806924873917
46-47	21.03981363023014	27.589040882166483	29.756898489817324	21.61424699778606
48-49	20.899337686727794	27.569400002340295	29.571789577710717	21.959472733221197
50-51	20.675208968132235	27.678508487488646	29.561726070160475	22.084556474218648
52-53	20.605529760054754	27.743344137666824	29.797375699030482	21.85375040324794
54-55	20.65313538204005	28.064616970564355	29.322805350537536	21.959442296858064
56-57	20.786431661232953	27.911341165367936	29.038256086411778	22.263971086987336
58-59	20.43094981416671	27.793454680232415	29.487720770975073	22.287874734625802
60-61	20.69804789407787	28.344773583320702	28.732289398035743	22.22488912456568
62-63	20.336643076922783	28.541960135238693	28.687981312638012	22.433415475200515
64-65	20.360554175643706	28.263981465705175	28.736081607825152	22.639382750825973
66-67	20.44410530027039	28.28219501331218	28.491567176518203	22.782132509899228
68-69	20.720380721375832	28.315533951949757	28.343018360802834	22.621066965871574
70-71	20.718513108591786	28.051671967031282	28.307295761867834	22.922519162509094
72-73	20.590643865686634	27.976912476057127	28.388709941646646	23.043733716609598
74-75	20.70661036377469	27.870939950505537	28.328154391974714	23.094295293745056
76-77	20.730941605915802	27.894117971632998	28.17720765142211	23.19773277102909
78-79	20.832791848559566	27.84927385226951	28.196968915697905	23.120965383473017
80-81	20.697814865490447	27.680159006052346	28.244199717294666	23.37782641116254
82-83	20.56074339583762	27.630431951324645	28.39132682023841	23.417497832599324
84-85	20.62745789733706	27.47094047903436	28.33476560541537	23.56683601821321
86-87	20.74671688249369	27.566314259709934	28.263945513772775	23.423023344023605
88-89	20.66284052302617	27.359524217925223	28.279299923596994	23.698335335451613
90-91	20.600973430448054	27.145428530112415	28.316950932712288	23.93664710672725
92-93	20.62362350648968	27.169439394328588	28.29951737699678	23.90741972218496
94-95	20.443141155880763	26.90989829123026	28.55839891742018	24.088561635468796
96-97	20.482941656124787	26.960773167869966	28.53136672911088	24.024918446894368
98-99	20.538484524763263	26.968274168780827	28.448712749174053	24.04452855728186
100	20.592102233873458	26.91192861712034	28.38704693596341	24.108922213042796
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	410.0
1	305.0
2	293.0
3	391.5
4	274.5
5	201.5
6	522.0
7	1485.5
8	2640.5
9	3176.5
10	2616.5
11	1808.5
12	1765.0
13	2095.5
14	2421.0
15	2822.5
16	3338.0
17	3846.5
18	4441.5
19	5312.5
20	6472.5
21	8034.5
22	10371.5
23	13839.5
24	18809.5
25	25336.0
26	34192.0
27	45912.5
28	60106.0
29	76122.5
30	92005.0
31	106397.5
32	119326.5
33	131462.0
34	143893.5
35	154890.0
36	163401.5
37	173957.0
38	184667.0
39	190672.5
40	196271.5
41	201365.0
42	203017.0
43	203260.0
44	202148.0
45	199116.5
46	194976.5
47	188538.5
48	180584.0
49	173883.5
50	166581.5
51	157584.5
52	146690.0
53	136218.0
54	127118.5
55	117951.5
56	106920.5
57	94030.5
58	83332.5
59	74269.0
60	63664.5
61	52435.0
62	43273.0
63	34888.0
64	27202.5
65	21300.5
66	16266.5
67	12089.5
68	8978.5
69	6634.0
70	4934.0
71	3681.5
72	2542.5
73	1555.0
74	1022.5
75	668.5
76	403.5
77	247.5
78	143.0
79	73.5
80	42.0
81	20.5
82	10.0
83	9.0
84	7.0
85	2.5
86	0.5
87	2.0
88	2.5
89	1.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.041611219922242
2	0.0
3	0.0
4	9.513310453187472E-5
5	0.0
6	0.0
7	0.0
8	0.0012177037380079966
9	0.0
10-11	0.0
12-13	0.044198840365508994
14-15	9.513310453187472E-5
16-17	0.0
18-19	4.756655226593736E-5
20-21	9.513310453187472E-5
22-23	0.0
24-25	8.561979407868725E-5
26-27	6.659317317231231E-5
28-29	4.3761228084662377E-4
30-31	2.853993135956242E-5
32-33	2.1880614042331189E-4
34-35	4.756655226593736E-5
36-37	7.610648362549979E-5
38-39	3.42479176314749E-4
40-41	9.513310453187472E-5
42-43	5.612853167380609E-4
44-45	3.0442593450199915E-4
46-47	1.1415972543824968E-4
48-49	9.227911139591848E-4
50-51	5.42258695831686E-4
52-53	6.183651794571857E-4
54-55	4.3761228084662377E-4
56-57	5.517720062848734E-4
58-59	1.5221296725099958E-4
60-61	1.6172627770418704E-4
62-63	9.418177348655597E-4
64-65	4.4712559129981124E-4
66-67	0.0015411562934163705
68-69	0.0016553160188546203
70-71	4.4712559129981124E-4
72-73	0.001046464149850622
74-75	0.0010369508393974346
76-77	7.420382153486228E-4
78-79	0.003481871625866615
80-81	0.002758860031424367
82-83	0.0014174832575249334
84-85	0.003015719413660429
86-87	0.0015316429829631832
88-89	0.0021024416101544314
90-91	0.0029300996195817416
92-93	0.0026447003059861173
94-95	0.0038053241812749895
96-97	0.003225012243630553
98-99	0.0029586395509413038
100	0.0015221296725099955
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5255794.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.05036674539196
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.17755688789568	48.660732662951744
2	13.882465001223062	17.505890193143646
3	4.230600962595972	8.002228267352528
4	1.7678907632215548	4.458646439476397
5	0.8773079553449267	2.765729416657379
6	0.5213595863600733	1.9723147875737084
7	0.3278060508519697	1.4467804209302706
8	0.2289904065701674	1.1550343292340373
9	0.16042893453234042	0.9103592842952887
>10	0.7739128578139286	8.429329488621725
>50	0.037797410534560656	1.6244513554852296
>100	0.012918363706272248	1.33995870904928
>500	5.125611698926023E-4	0.2239008190377595
>1k	2.713549077412459E-4	0.3386446635526782
>5k	6.030109060690764E-5	0.19810351626863798
>10k+	1.2060218121381528E-4	0.9678956463696096
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT	19970	0.37996161950030766	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10419	0.19823836322352054	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10412	0.19810517687717596	No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	10114	0.1924352438470762	TruSeq Adapter, Index 11 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.9026620906374947E-5	0.0
2	0.0	0.0	0.0	1.9026620906374947E-5	0.0
3	0.0	0.0	0.0	1.9026620906374947E-5	0.0
4	0.0	0.0	0.0	1.1415972543824968E-4	0.0
5	0.0	0.0	0.0	1.5221296725099958E-4	0.0
6	0.0	0.0	0.0	1.5221296725099958E-4	0.0
7	0.0	0.0	0.0	1.5221296725099958E-4	0.0
8	0.0	0.0	0.0	1.712395881573745E-4	0.0
9	0.0	0.0	0.0	1.9026620906374946E-4	0.0
10-11	0.0	0.0	0.0	2.3783276132968682E-4	0.0
12-13	0.0	0.0	0.0	2.6637269268924924E-4	0.0
14-15	0.0	0.0	0.0	2.949126240488117E-4	0.0
16-17	0.0	0.0	0.0	3.900457285806864E-4	9.513310453187474E-6
18-19	0.0	0.0	0.0	5.517720062848735E-4	1.9026620906374947E-5
20-21	0.0	0.0	0.0	8.657112512400599E-4	1.9026620906374947E-5
22-23	0.0	0.0	0.0	0.002092928299701244	1.9026620906374947E-5
24-25	0.0	0.0	0.0	0.005042054540189361	1.9026620906374947E-5
26-27	0.0	0.0	0.0	0.010036542528112785	1.9026620906374947E-5
28-29	0.0	0.0	0.0	0.029148783228566417	1.9026620906374947E-5
30-31	0.0	0.0	0.0	0.08327951970720313	1.9026620906374947E-5
32-33	0.0	0.0	0.0	0.20657202318051276	1.9026620906374947E-5
34-35	0.0	0.0	0.0	0.4001488642819715	1.9026620906374947E-5
36-37	0.0	0.0	0.0	0.6991712384465602	1.9026620906374947E-5
38-39	0.0	0.0	0.0	1.0853165097414397	1.9026620906374947E-5
40-41	9.513310453187474E-6	0.0	0.0	1.4679799094104524	1.9026620906374947E-5
42-43	2.8539931359562422E-5	0.0	0.0	1.8467523651041118	1.9026620906374947E-5
44-45	3.8053241812749894E-5	0.0	0.0	2.292850518874979	1.9026620906374947E-5
46-47	3.8053241812749894E-5	0.0	0.0	2.8046856478773714	1.9026620906374947E-5
48-49	3.8053241812749894E-5	0.0	0.0	3.313600190570635	1.9026620906374947E-5
50-51	3.8053241812749894E-5	0.0	0.0	3.802631914416737	1.9026620906374947E-5
52-53	3.8053241812749894E-5	0.0	0.0	4.260916618878137	1.9026620906374947E-5
54-55	3.8053241812749894E-5	0.0	0.0	4.751166046462247	1.9026620906374947E-5
56-57	3.8053241812749894E-5	0.0	0.0	5.305230760566339	1.9026620906374947E-5
58-59	3.8053241812749894E-5	0.0	0.0	5.883687602672403	1.9026620906374947E-5
60-61	3.8053241812749894E-5	0.0	0.0	6.444611413613242	1.9026620906374947E-5
62-63	3.8053241812749894E-5	0.0	0.0	6.9732565621864175	1.9026620906374947E-5
64-65	3.8053241812749894E-5	0.0	0.0	7.514925432770006	1.9026620906374947E-5
66-67	3.8053241812749894E-5	0.0	0.0	8.104408201691314	1.9026620906374947E-5
68-69	3.8053241812749894E-5	0.0	0.0	8.72229771562584	1.9026620906374947E-5
70-71	3.8053241812749894E-5	0.0	0.0	9.34777885130201	1.9026620906374947E-5
72-73	3.8053241812749894E-5	0.0	0.0	9.956117001541537	1.9026620906374947E-5
74-75	3.8053241812749894E-5	0.0	0.0	10.544648439417527	1.9026620906374947E-5
76-77	3.8053241812749894E-5	0.0	0.0	11.15995984621924	1.9026620906374947E-5
78-79	3.8053241812749894E-5	0.0	0.0	11.814256799258114	1.9026620906374947E-5
80-81	3.8053241812749894E-5	0.0	0.0	12.481767740516466	1.9026620906374947E-5
82-83	3.8053241812749894E-5	0.0	0.0	13.13871890717178	1.9026620906374947E-5
84-85	3.8053241812749894E-5	0.0	0.0	13.766121731559494	1.9026620906374947E-5
86-87	3.8053241812749894E-5	0.0	0.0	14.403713311442573	1.9026620906374947E-5
88	3.8053241812749894E-5	0.0	0.0	14.903171623545367	1.9026620906374947E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8425	0.0	58.540794	1
GTATCAA	15190	0.0	39.433445	1
ATCAACG	18585	0.0	32.06445	3
TCAACGC	18585	0.0	32.06445	4
CAACGCA	19285	0.0	30.924955	5
TATCAAC	19390	0.0	30.709015	2
AACGCAG	20150	0.0	29.738071	6
GTGGTAT	3665	0.0	28.351194	1
TGGTATC	3710	0.0	27.235266	2
ACGCAGA	22825	0.0	25.985249	7
CGCAGAG	23005	0.0	25.598164	8
GCAGAGT	24980	0.0	23.21609	9
GAGTACT	16950	0.0	21.469109	12-13
GTACATG	13265	0.0	21.231106	1
CAGAGTA	24365	0.0	20.838894	10-11
TACATGG	13295	0.0	20.290401	2
AGAGTAC	22995	0.0	20.116821	10-11
GTACTTT	18785	0.0	19.101114	14-15
ACATGGG	13715	0.0	18.949442	3
AGTACTT	18070	0.0	18.382168	12-13
>>END_MODULE