FastQCFastQC Report
Wed 25 May 2016
SRR2049368_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049368_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6487389
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58500.042.5923961
GTATCAA92800.034.1447561
TCAACGC97250.031.0339784
ATCAACG98300.030.8459513
CAACGCA103950.029.2146035
AACGCAG109500.027.6437386
TATCAAC114150.026.8511962
GTACATG149300.024.4350911
ACGCAGA125750.023.6595957
TACATGG149900.023.0817182
GCCGTAT15550.022.97001694
CGCAGAG129400.022.8106148
ACATGGG157650.021.5295623
GAGTACA116850.019.9958441
GTGGTAT38700.019.5581151
CATGGGG109600.019.4303054
GCAGAGT149150.019.0022779
GCCGGAT15100.018.0521694
CAGAGTA140150.017.28809510-11
AGTACAT121300.017.1298452
Produced by FastQC (version 0.11.5)