##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049368_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6487389 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.388969583911184 31.0 31.0 34.0 27.0 34.0 2 30.60946769185569 31.0 31.0 34.0 27.0 34.0 3 30.65630394600971 31.0 31.0 34.0 27.0 34.0 4 34.27049742199828 37.0 35.0 37.0 30.0 37.0 5 34.30184624353495 37.0 35.0 37.0 30.0 37.0 6 34.2840193489245 37.0 35.0 37.0 30.0 37.0 7 34.226290114559184 37.0 35.0 37.0 30.0 37.0 8 34.18330224994986 37.0 35.0 37.0 30.0 37.0 9 35.71167799556956 39.0 37.0 39.0 30.0 39.0 10-11 35.66728016463942 39.0 36.0 39.0 30.0 39.0 12-13 35.61159651440664 39.0 35.0 39.0 30.0 39.0 14-15 36.756262342215024 40.0 37.0 41.0 30.0 41.0 16-17 36.71741304552571 40.0 37.0 41.0 30.0 41.0 18-19 36.73716929877336 40.0 37.0 41.0 30.0 41.0 20-21 36.812366808896456 40.0 37.0 41.0 30.0 41.0 22-23 36.745157797690254 40.0 37.0 41.0 30.0 41.0 24-25 36.73999454942505 40.0 37.0 41.0 30.0 41.0 26-27 36.71657526625889 40.0 37.0 41.0 30.0 41.0 28-29 36.63935598743963 40.0 37.0 41.0 30.0 41.0 30-31 36.561157963550514 40.0 37.0 41.0 30.0 41.0 32-33 36.49493987488649 40.0 36.5 41.0 30.0 41.0 34-35 36.38360956927355 40.0 36.0 41.0 30.0 41.0 36-37 36.288452642503785 40.0 36.0 41.0 30.0 41.0 38-39 36.176542442575894 40.0 36.0 41.0 29.0 41.0 40-41 36.07115759514343 40.0 36.0 41.0 29.0 41.0 42-43 35.9248128946792 39.5 35.5 41.0 28.0 41.0 44-45 35.79597832039978 39.0 35.0 41.0 28.0 41.0 46-47 35.68001386998684 39.0 35.0 41.0 28.0 41.0 48-49 35.55418558991915 39.0 35.0 41.0 27.0 41.0 50-51 35.420969746071954 39.0 35.0 41.0 27.0 41.0 52-53 35.273272729598915 39.0 35.0 40.5 26.5 41.0 54-55 35.131260049304885 39.0 35.0 40.0 26.0 41.0 56-57 34.99996531732566 39.0 34.5 40.0 26.0 41.0 58-59 34.80254521503181 38.5 34.0 40.0 26.0 41.0 60-61 34.578464695118484 38.0 34.0 40.0 25.0 41.0 62-63 34.35755625260023 38.0 34.0 40.0 25.0 41.0 64-65 34.09633652306036 37.5 34.0 40.0 24.0 41.0 66-67 33.80889869560774 37.0 33.5 40.0 23.0 41.0 68-69 33.4689379656438 36.5 33.0 39.5 22.5 41.0 70-71 33.119446436771405 36.0 33.0 39.0 22.0 41.0 72-73 32.716784441321465 36.0 32.5 39.0 20.0 40.5 74-75 32.31726292349665 35.0 32.0 38.5 19.0 40.0 76-77 31.917493771377053 35.0 32.0 37.5 17.5 40.0 78-79 31.51454653019882 35.0 32.0 37.0 12.0 39.0 80-81 31.062554056801588 35.0 31.0 36.5 7.5 39.0 82-83 30.637197877297012 35.0 31.0 36.0 2.0 38.5 84-85 30.243279538193256 35.0 31.0 36.0 2.0 37.0 86-87 29.82159193783508 34.5 30.5 35.0 2.0 37.0 88-89 29.438369889642814 34.0 30.0 35.0 2.0 36.5 90-91 29.087958961609978 34.0 30.0 35.0 2.0 36.0 92-93 28.69574030476668 34.0 29.0 35.0 2.0 36.0 94-95 28.33523841409849 34.0 29.0 35.0 2.0 36.0 96-97 27.927740189466057 34.0 29.0 35.0 2.0 35.0 98-99 27.570941560618607 34.0 29.0 35.0 2.0 35.0 100 27.34556598964545 34.0 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.47273629891878954 1101 2 0.4575126537335841 1101 3 0.45470420321391813 1101 4 0.506788257231328 1101 5 0.45790250695134205 1101 6 0.5848908179819148 1101 7 0.5626851458811899 1101 8 0.5722835122399417 1101 9 0.7199713375488983 1101 10-11 0.6978001195282744 1101 12-13 0.6972896517688412 1101 14-15 0.8231296813522846 1101 16-17 0.8876437081170394 1101 18-19 0.8061463960702895 1101 20-21 0.786814024670754 1101 22-23 0.8137074394643022 1101 24-25 0.8549597110077514 1101 26-27 0.734736939872235 1101 28-29 0.7907867037253098 1101 30-31 0.7950976422421476 1101 32-33 0.7350374153013775 1101 34-35 0.7854922599174543 1101 36-37 0.7607321229891895 1101 38-39 0.7741451224703226 1101 40-41 0.8285196754662465 1101 42-43 0.8634847963361878 1101 44-45 0.7920330182144468 1101 46-47 0.78111284914349 1101 48-49 0.7357648149008753 1101 50-51 0.7764926153386398 1101 52-53 0.7874379986701712 1101 54-55 0.7791030038719171 1101 56-57 0.7497755009546623 1101 58-59 0.8411062859191745 1101 60-61 0.8536237782811824 1101 62-63 0.7974260952628072 1101 64-65 0.7764467464185216 1101 66-67 0.8073668334990813 1101 68-69 0.7870728742619235 1101 70-71 0.7924807223259833 1101 72-73 0.7960851711573724 1101 74-75 0.7712637057372547 1101 76-77 0.8271161762067756 1101 78-79 0.8135588932975715 1101 80-81 0.8355206945901408 1101 82-83 0.8228272423454932 1101 84-85 0.7567439931810434 1101 86-87 0.81991576433105 1101 88-89 0.7600455231020291 1101 90-91 0.81009809589602 1101 92-93 0.8214379687484055 1101 94-95 0.8142531116418894 1101 96-97 0.8200579591096187 1101 98-99 0.8992817585737463 1101 100 0.9741209227999228 1102 1 0.3183406754502087 1102 2 0.3691384661832622 1102 3 0.4067878148157469 1102 4 0.445139227441949 1102 5 0.34769424766572854 1102 6 0.4844961106298129 1102 7 0.5058205608263648 1102 8 0.5185526140357766 1102 9 0.6611513896533339 1102 10-11 0.5096158840264664 1102 12-13 0.6600202498832317 1102 14-15 0.7151975200040965 1102 16-17 0.6986081471013463 1102 18-19 0.7175927583558703 1102 20-21 0.628581716950201 1102 22-23 0.6311304367549369 1102 24-25 0.7049659429643214 1102 26-27 0.6465453467627782 1102 28-29 0.6764827786868821 1102 30-31 0.643944887490953 1102 32-33 0.7173995884443656 1102 34-35 0.7199205373291946 1102 36-37 0.6831370444615033 1102 38-39 0.7318734403551943 1102 40-41 0.7690750666809336 1102 42-43 0.7073750200753679 1102 44-45 0.6479789210583036 1102 46-47 0.5940461650701039 1102 48-49 0.6370856060914676 1102 50-51 0.7694876005944948 1102 52-53 0.7628513327178865 1102 54-55 0.7156700623657741 1102 56-57 0.6280976054467757 1102 58-59 0.7228211027826603 1102 60-61 0.7292224108477896 1102 62-63 0.7173543768249928 1102 64-65 0.709113103185274 1102 66-67 0.814175425273099 1102 68-69 0.8010401352975123 1102 70-71 0.7511926831799869 1102 72-73 0.7198700402435207 1102 74-75 0.720049819957012 1102 76-77 0.8209436024502175 1102 78-79 0.71906452193031 1102 80-81 0.7015307608035783 1102 82-83 0.6723250447475699 1102 84-85 0.6923375521426465 1102 86-87 0.6559729469031019 1102 88-89 0.6746487078267798 1102 90-91 0.7240370835948546 1102 92-93 0.6747444210307343 1102 94-95 0.7026243498605993 1102 96-97 0.6768335611317156 1102 98-99 0.7056643511532066 1102 100 0.6816939511434406 1103 1 0.26907944764176506 1103 2 0.32385735236213264 1103 3 0.2922647789985895 1103 4 0.2717349360972534 1103 5 0.3189514418094461 1103 6 0.3960217324132387 1103 7 0.42438970746130167 1103 8 0.46234563006029106 1103 9 0.5838306443923642 1103 10-11 0.5834470804202212 1103 12-13 0.5035380982369517 1103 14-15 0.5993095400238531 1103 16-17 0.630275073724583 1103 18-19 0.6318173135273568 1103 20-21 0.5995967335267025 1103 22-23 0.600004545930716 1103 24-25 0.6868087582293896 1103 26-27 0.6168942800392259 1103 28-29 0.5839949508524782 1103 30-31 0.5780250466033792 1103 32-33 0.6760476204203627 1103 34-35 0.6915646800730926 1103 36-37 0.6204615826819833 1103 38-39 0.6184628112629866 1103 40-41 0.626783830606918 1103 42-43 0.6798472994355294 1103 44-45 0.5191055107730804 1103 46-47 0.5932367771486184 1103 48-49 0.6598933695376985 1103 50-51 0.6451069843273913 1103 52-53 0.68896707834908 1103 54-55 0.6601540966232378 1103 56-57 0.5720743028733324 1103 58-59 0.7016205619938347 1103 60-61 0.645860424110225 1103 62-63 0.6953675663596002 1103 64-65 0.6658059811367565 1103 66-67 0.7162421939940984 1103 68-69 0.7806913124472032 1103 70-71 0.7622753465670868 1103 72-73 0.7563976508846864 1103 74-75 0.7695126704453941 1103 76-77 0.7818492551235146 1103 78-79 0.6386287979891812 1103 80-81 0.6127687165779392 1103 82-83 0.5947268864844553 1103 84-85 0.7297680626405167 1103 86-87 0.7245360569095212 1103 88-89 0.701625057731615 1103 90-91 0.7850029766912456 1103 92-93 0.6669179570972616 1103 94-95 0.7907196874128601 1103 96-97 0.7426102517569753 1103 98-99 0.742545456026253 1103 100 0.7694588117816252 1104 1 0.17046943033257378 1104 2 0.18371565377493937 1104 3 0.221141183111893 1104 4 0.2266553421786739 1104 5 0.274217553961968 1104 6 0.30445095370650677 1104 7 0.3374501912179042 1104 8 0.378303944450181 1104 9 0.4871223879441047 1104 10-11 0.44191323911308444 1104 12-13 0.381280438040676 1104 14-15 0.4924372535322803 1104 16-17 0.5729473653159403 1104 18-19 0.506972717031779 1104 20-21 0.46820302898287736 1104 22-23 0.49587642039418967 1104 24-25 0.5489325831557892 1104 26-27 0.4596902837216632 1104 28-29 0.5046130069630479 1104 30-31 0.4461505975474225 1104 32-33 0.49147537328818913 1104 34-35 0.515497071589401 1104 36-37 0.5193795771027112 1104 38-39 0.5942688736839088 1104 40-41 0.5775810712187166 1104 42-43 0.5350442703738381 1104 44-45 0.5196529998473807 1104 46-47 0.5164701699415346 1104 48-49 0.46607414698435434 1104 50-51 0.4545213265129391 1104 52-53 0.5048863815085838 1104 54-55 0.5604234106780979 1104 56-57 0.5081162684196201 1104 58-59 0.5507141379376108 1104 60-61 0.5315684657161555 1104 62-63 0.5330757267727577 1104 64-65 0.5693947258773449 1104 66-67 0.6196386319156275 1104 68-69 0.5334920516301267 1104 70-71 0.5326357986398875 1104 72-73 0.6022082698741045 1104 74-75 0.622935876615287 1104 76-77 0.6840007072394307 1104 78-79 0.5873493289015848 1104 80-81 0.51911827601932 1104 82-83 0.490413378165929 1104 84-85 0.5114150894742195 1104 86-87 0.5562677817869819 1104 88-89 0.5232896665489299 1104 90-91 0.5692680726385113 1104 92-93 0.6056338911025918 1104 94-95 0.6131003453243835 1104 96-97 0.5120422913079743 1104 98-99 0.46036826429556754 1104 100 0.48253780395687684 1105 1 0.2969469089254595 1105 2 0.29047447191189235 1105 3 0.3007730301947298 1105 4 0.42152354295716066 1105 5 0.4132821696046065 1105 6 0.40685081944918267 1105 7 0.42058979291839194 1105 8 0.46468691277105023 1105 9 0.5718516390317774 1105 10-11 0.5012432470770918 1105 12-13 0.47209257814029826 1105 14-15 0.5027831320063072 1105 16-17 0.4899137281375445 1105 18-19 0.47838715319527836 1105 20-21 0.4738603244463917 1105 22-23 0.47124320400666164 1105 24-25 0.4360750486110305 1105 26-27 0.4491669130711671 1105 28-29 0.49144604469324094 1105 30-31 0.4941646742752468 1105 32-33 0.5009379720036975 1105 34-35 0.5912211858968206 1105 36-37 0.5267587373115532 1105 38-39 0.5473529960900052 1105 40-41 0.5708123550854864 1105 42-43 0.5210927893733199 1105 44-45 0.47590575729319085 1105 46-47 0.4802482133342849 1105 48-49 0.5035713356725324 1105 50-51 0.5063175955044699 1105 52-53 0.4956436201953167 1105 54-55 0.512105324064585 1105 56-57 0.4331867171518127 1105 58-59 0.4261939859728301 1105 60-61 0.4076923937131198 1105 62-63 0.3983207550988297 1105 64-65 0.40391505694161367 1105 66-67 0.4599614283435045 1105 68-69 0.4292873287715082 1105 70-71 0.4798086289861274 1105 72-73 0.43845677207860945 1105 74-75 0.5122145652627808 1105 76-77 0.5842411170523683 1105 78-79 0.5160476581277145 1105 80-81 0.6039139124596815 1105 82-83 0.5699785782420967 1105 84-85 0.5994979382662109 1105 86-87 0.606840482008959 1105 88-89 0.5457592287953084 1105 90-91 0.5729926636474403 1105 92-93 0.6098328340781833 1105 94-95 0.5493200517073937 1105 96-97 0.5569628704073963 1105 98-99 0.536206134005834 1105 100 0.5702631746733076 1106 1 0.17149533880436607 1106 2 0.18086059616451067 1106 3 0.20714117436254398 1106 4 0.24464943959873864 1106 5 0.2174765518473194 1106 6 0.29852355247499673 1106 7 0.27193302475757264 1106 8 0.27725590977497205 1106 9 0.372993299009174 1106 10-11 0.3564954405080343 1106 12-13 0.32576113994918643 1106 14-15 0.39382630618005976 1106 16-17 0.4802226531897418 1106 18-19 0.45504174864630187 1106 20-21 0.36138443094113626 1106 22-23 0.4533342072205713 1106 24-25 0.4167082075281172 1106 26-27 0.3429915139001736 1106 28-29 0.416784610373405 1106 30-31 0.44759174869161455 1106 32-33 0.4251411812142152 1106 34-35 0.4684336371563589 1106 36-37 0.4381585184752339 1106 38-39 0.48918960231927144 1106 40-41 0.5330291460360215 1106 42-43 0.5528643197270853 1106 44-45 0.43693562930688756 1106 46-47 0.4506098223000734 1106 48-49 0.465605763027348 1106 50-51 0.47960101247506515 1106 52-53 0.47970312485603017 1106 54-55 0.5042503347332925 1106 56-57 0.4174238235807266 1106 58-59 0.4900378954468039 1106 60-61 0.5495037725412146 1106 62-63 0.45672728490344383 1106 64-65 0.428896061826471 1106 66-67 0.5049949311941262 1106 68-69 0.5298063093491905 1106 70-71 0.5334362277650726 1106 72-73 0.6179896053268124 1106 74-75 0.6297177504677265 1106 76-77 0.6525204837441159 1106 78-79 0.4655354258721367 1106 80-81 0.5198493240595567 1106 82-83 0.47687002462890504 1106 84-85 0.4601760593255868 1106 86-87 0.48068381173300523 1106 88-89 0.5196266998536032 1106 90-91 0.5903565391711325 1106 92-93 0.526466254515249 1106 94-95 0.5805554445057624 1106 96-97 0.6035816658631177 1106 98-99 0.6050080984129309 1106 100 0.5660790401302691 1107 1 0.1737703484508515 1107 2 0.15777295285187876 1107 3 0.16102322761884125 1107 4 0.18218284361151404 1107 5 0.17524437147542926 1107 6 0.24091832242054068 1107 7 0.18352713077481297 1107 8 0.25338210993486143 1107 9 0.30050269381262495 1107 10-11 0.26006822009701835 1107 12-13 0.19981045312901102 1107 14-15 0.252767858054753 1107 16-17 0.2940165226576781 1107 18-19 0.2135331358614465 1107 20-21 0.22831687592410077 1107 22-23 0.2152556926014313 1107 24-25 0.24784737613970975 1107 26-27 0.24189761063146875 1107 28-29 0.2719009118442415 1107 30-31 0.26981458575874484 1107 32-33 0.2708911738400843 1107 34-35 0.2558982814034252 1107 36-37 0.21903705663548578 1107 38-39 0.22278626327258877 1107 40-41 0.23632338284790677 1107 42-43 0.22253940648935355 1107 44-45 0.18671249671344015 1107 46-47 0.19010426529106184 1107 48-49 0.22840083247482568 1107 50-51 0.31370523307509046 1107 52-53 0.2898278981616045 1107 54-55 0.32211235508012237 1107 56-57 0.259141003046075 1107 58-59 0.3128501019343375 1107 60-61 0.31087623081045024 1107 62-63 0.2685793482494816 1107 64-65 0.3074291122817243 1107 66-67 0.24117995227371836 1107 68-69 0.2879506314765692 1107 70-71 0.20532057818346772 1107 72-73 0.25467617052542835 1107 74-75 0.3064962446316599 1107 76-77 0.3459288059801757 1107 78-79 0.23428596290125725 1107 80-81 0.19657343901552693 1107 82-83 0.23104227499572616 1107 84-85 0.222508076416581 1107 86-87 0.2962778208445478 1107 88-89 0.33268364714283294 1107 90-91 0.3213411746477881 1107 92-93 0.23900909962018346 1107 94-95 0.21094012828297792 1107 96-97 0.14305662752164494 1107 98-99 0.10803751374211501 1107 100 0.12112108830803336 1108 1 -0.0860539254738164 1108 2 -0.012989676066016642 1108 3 -0.030304085852346674 1108 4 -0.002430517852943126 1108 5 0.02518679486606601 1108 6 0.16150101087849578 1108 7 0.0712845071506294 1108 8 0.04433135126676291 1108 9 0.10407280836407296 1108 10-11 -0.013305165269592578 1108 12-13 0.08357287426556326 1108 14-15 0.10700587685153096 1108 16-17 0.09933338664761493 1108 18-19 0.05465420298133239 1108 20-21 0.1115452824379517 1108 22-23 0.0759313396496637 1108 24-25 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44-45 0.3785415809255497 2211 46-47 0.3322581369261073 2211 48-49 0.34363152409427045 2211 50-51 0.28985363836942923 2211 52-53 0.2826949483572818 2211 54-55 0.2976648284748009 2211 56-57 0.27530106205460214 2211 58-59 0.4749490146210249 2211 60-61 0.3716913978873535 2211 62-63 0.3888743839036337 2211 64-65 0.4392033882495383 2211 66-67 0.3301415965129806 2211 68-69 0.06743730227054812 2211 70-71 0.26802145276570855 2211 72-73 0.31208101649340847 2211 74-75 0.434199109167551 2211 76-77 0.4075411553824573 2211 78-79 0.3161257401349822 2211 80-81 0.34220839116716917 2211 82-83 0.4140156804587356 2211 84-85 0.3899108370950053 2211 86-87 0.45537769517715887 2211 88-89 0.47874743668926456 2211 90-91 0.439398493306431 2211 92-93 0.4613463880001447 2211 94-95 0.3383337103139503 2211 96-97 0.43098067245389515 2211 98-99 0.367151314450183 2211 100 0.3990654166871259 2212 1 -0.20263280356224556 2212 2 -0.17752992780762966 2212 3 -0.33733749806983226 2212 4 -0.2793485507321307 2212 5 -0.2975565898432393 2212 6 -0.34107494231967195 2212 7 -0.4032602570088102 2212 8 -0.376986974971139 2212 9 -0.47219136998062794 2212 10-11 -0.5099059506718291 2212 12-13 -0.5729190832276387 2212 14-15 -0.3987827118051257 2212 16-17 -0.4054096594936212 2212 18-19 -0.34608190238006387 2212 20-21 -0.35887629792393483 2212 22-23 -0.31027858899471283 2212 24-25 -0.39465782559928186 2212 26-27 -0.4299668506149885 2212 28-29 -0.4725607190142469 2212 30-31 -0.45296643664484293 2212 32-33 -0.4237832978206839 2212 34-35 -0.5419116585266366 2212 36-37 -0.45008150018069415 2212 38-39 -0.5853550573596991 2212 40-41 -0.5360964158143204 2212 42-43 -0.4959627328708436 2212 44-45 -0.4720765634992148 2212 46-47 -0.43852121503211805 2212 48-49 -0.3161361975740249 2212 50-51 -0.2803649417128824 2212 52-53 -0.1982550419779443 2212 54-55 -0.36009667555949676 2212 56-57 -0.4431864200187121 2212 58-59 -0.3195904749479439 2212 60-61 -0.2218882863462852 2212 62-63 -0.20630953330181967 2212 64-65 -0.23594012899091155 2212 66-67 -0.2708781032323131 2212 68-69 -0.273915999600959 2212 70-71 -0.14021131833349187 2212 72-73 -0.2429845921620597 2212 74-75 -0.1750246508091564 2212 76-77 -0.13568415275854662 2212 78-79 -0.1862722079821033 2212 80-81 -0.29096606741675757 2212 82-83 -0.21701270349247537 2212 84-85 -0.28085506688249495 2212 86-87 -0.2858735184216137 2212 88-89 -0.34187592919683496 2212 90-91 -0.36784682869121355 2212 92-93 -0.42246608894150484 2212 94-95 -0.4417937097827789 2212 96-97 -0.23396338382957538 2212 98-99 -0.2436438776303511 2212 100 -0.3307945710842226 2213 1 -0.39238021365285647 2213 2 -0.37969271797707904 2213 3 -0.4952741816471189 2213 4 -0.53872848404243 2213 5 -0.42622361389820185 2213 6 -0.3508913014173274 2213 7 -0.38059003575570216 2213 8 -0.303948480954773 2213 9 -0.39094584160574186 2213 10-11 -0.35486997767012696 2213 12-13 -0.4191339491738617 2213 14-15 -0.5666389669345477 2213 16-17 -0.5454876979826153 2213 18-19 -0.4803130441673389 2213 20-21 -0.3383554798544779 2213 22-23 -0.36706624305811886 2213 24-25 -0.40882084652337625 2213 26-27 -0.4305449633756169 2213 28-29 -0.4231203372318859 2213 30-31 -0.5263169640136951 2213 32-33 -0.5803665088922401 2213 34-35 -0.5056449752970948 2213 36-37 -0.5138269979021928 2213 38-39 -0.523455178747092 2213 40-41 -0.5150511454906805 2213 42-43 -0.3430683559877892 2213 44-45 -0.36158675057329503 2213 46-47 -0.5470836115518196 2213 48-49 -0.6293553234360161 2213 50-51 -0.5224787829516728 2213 52-53 -0.5183657358680307 2213 54-55 -0.4875511120689069 2213 56-57 -0.45645105359218974 2213 58-59 -0.4558313369733469 2213 60-61 -0.3958029324524688 2213 62-63 -0.34685258663207463 2213 64-65 -0.4082971490478755 2213 66-67 -0.3022626411833116 2213 68-69 -0.38480401706273426 2213 70-71 -0.43026063510474444 2213 72-73 -0.44693146552700824 2213 74-75 -0.39347740067895387 2213 76-77 -0.32690242675798586 2213 78-79 -0.540038005278614 2213 80-81 -0.6169123333421958 2213 82-83 -0.6331141318195357 2213 84-85 -0.5402814847924304 2213 86-87 -0.4976995977573928 2213 88-89 -0.5339599713268406 2213 90-91 -0.4974340130442805 2213 92-93 -0.5114834218720219 2213 94-95 -0.5542417774236377 2213 96-97 -0.5792751789902013 2213 98-99 -0.6586164132038554 2213 100 -0.6325231525602142 2214 1 -0.18131477219789716 2214 2 -0.19029333453098474 2214 3 -0.22465156825823485 2214 4 -0.1718062817346393 2214 5 -0.20405480647849572 2214 6 -0.20582919993577775 2214 7 -0.27585855641920176 2214 8 -0.20259904886444247 2214 9 -0.2676151499827526 2214 10-11 -0.35979225622948263 2214 12-13 -0.33091188216420875 2214 14-15 -0.3953911177996261 2214 16-17 -0.39988537931570534 2214 18-19 -0.4142517336506373 2214 20-21 -0.41821843992091345 2214 22-23 -0.38714498344418047 2214 24-25 -0.39372744512874647 2214 26-27 -0.39369858105762034 2214 28-29 -0.3475538974426584 2214 30-31 -0.4101475949644353 2214 32-33 -0.34115847623489515 2214 34-35 -0.3844034991674121 2214 36-37 -0.4128922620819324 2214 38-39 -0.36243729001360947 2214 40-41 -0.34189894252681796 2214 42-43 -0.2719198661268294 2214 44-45 -0.335353955316684 2214 46-47 -0.2601759821955838 2214 48-49 -0.24176776235009356 2214 50-51 -0.16236388306669625 2214 52-53 -0.16096116886370737 2214 54-55 -0.18952974343844886 2214 56-57 -0.26207766438233193 2214 58-59 -0.21176044778912484 2214 60-61 -0.24639944225960164 2214 62-63 -0.2112025139705267 2214 64-65 -0.15894218117126258 2214 66-67 -0.22544401520944035 2214 68-69 -0.2923880558682015 2214 70-71 -0.3377805529233626 2214 72-73 -0.4591207737801568 2214 74-75 -0.32562673298424727 2214 76-77 -0.27194920825486335 2214 78-79 -0.2536246138005609 2214 80-81 -0.15712234641140554 2214 82-83 -0.10689696619862232 2214 84-85 -0.13024594653088428 2214 86-87 -0.11829554438671508 2214 88-89 -0.03809553112848718 2214 90-91 -0.1375136243238586 2214 92-93 -0.13369302968544616 2214 94-95 -0.12893570189626757 2214 96-97 -0.17051779502273234 2214 98-99 -0.15030246499260258 2214 100 -0.18906492341961467 2215 1 -0.3618345143859365 2215 2 -0.40126088918737324 2215 3 -0.41793390927607277 2215 4 -0.3604723021117522 2215 5 -0.3378124581517383 2215 6 -0.3801224911682297 2215 7 -0.5064390761613851 2215 8 -0.36974386025824657 2215 9 -0.48214240129557595 2215 10-11 -0.5689631119501755 2215 12-13 -0.5358856664179399 2215 14-15 -0.73534080836491 2215 16-17 -0.6915352390746037 2215 18-19 -0.7260906022220794 2215 20-21 -0.6767223390419446 2215 22-23 -0.6950543859083496 2215 24-25 -0.7132736720201933 2215 26-27 -0.664521381304624 2215 28-29 -0.7046706279866441 2215 30-31 -0.6798115372164943 2215 32-33 -0.6609771678936056 2215 34-35 -0.6224842988790655 2215 36-37 -0.6776304431462563 2215 38-39 -0.5046522219591836 2215 40-41 -0.7145455588351055 2215 42-43 -0.5965090509967581 2215 44-45 -0.565452205079275 2215 46-47 -0.6445395740226019 2215 48-49 -0.6074832436883995 2215 50-51 -0.5624262862499876 2215 52-53 -0.5673557762964094 2215 54-55 -0.6614959727700906 2215 56-57 -0.6062417081925133 2215 58-59 -0.5990449048051047 2215 60-61 -0.43067562301698104 2215 62-63 -0.5204504559764658 2215 64-65 -0.46535070259105993 2215 66-67 -0.4555759428804791 2215 68-69 -0.45854159473466893 2215 70-71 -0.4809244124792613 2215 72-73 -0.5691368401235337 2215 74-75 -0.5671603107329624 2215 76-77 -0.6019069314091716 2215 78-79 -0.6228823002587696 2215 80-81 -0.6470900820428547 2215 82-83 -0.6332255392936084 2215 84-85 -0.6008893216026863 2215 86-87 -0.5399431212728523 2215 88-89 -0.6070683187958004 2215 90-91 -0.6472691122206129 2215 92-93 -0.6140391034065011 2215 94-95 -0.673938500425578 2215 96-97 -0.676349829892068 2215 98-99 -0.7800692495552539 2215 100 -0.7895256698362658 2216 1 -1.3541272318817263 2216 2 -1.2903214319692857 2216 3 -1.3402718313796136 2216 4 -1.5446963223403074 2216 5 -1.477715162001509 2216 6 -2.871625124207622 2216 7 -2.718167526004766 2216 8 -3.6240225457645074 2216 9 -3.1550280540094846 2216 10-11 -2.239423247843021 2216 12-13 -2.0315593229500806 2216 14-15 -2.3436095906829877 2216 16-17 -2.716859431261888 2216 18-19 -2.387560297422958 2216 20-21 -2.1824481580389303 2216 22-23 -2.364590790683927 2216 24-25 -2.561551392422402 2216 26-27 -2.5275737463647943 2216 28-29 -3.0318337110676197 2216 30-31 -2.6178613849993724 2216 32-33 -2.765577584301198 2216 34-35 -3.6211767517971154 2216 36-37 -2.7034688924549215 2216 38-39 -2.6282809590661245 2216 40-41 -3.265324729646295 2216 42-43 -3.0114411386347726 2216 44-45 -2.6532047481825742 2216 46-47 -2.5524925325256405 2216 48-49 -2.8319308074725598 2216 50-51 -3.0734808709934853 2216 52-53 -3.4208955954238576 2216 54-55 -2.77227504746282 2216 56-57 -2.7723161031384294 2216 58-59 -3.663080505778346 2216 60-61 -4.41615404521464 2216 62-63 -3.5600242993610394 2216 64-65 -3.5591325517479646 2216 66-67 -3.17772914022391 2216 68-69 -3.561568556524218 2216 70-71 -3.313633708338706 2216 72-73 -3.7094438247724852 2216 74-75 -4.133337323805911 2216 76-77 -4.7569114279803095 2216 78-79 -3.8100961180254345 2216 80-81 -3.6085134304126605 2216 82-83 -3.820020817133365 2216 84-85 -3.9039341212997343 2216 86-87 -3.483239278223298 2216 88-89 -3.431805843062218 2216 90-91 -3.5867142035205575 2216 92-93 -3.549024372551738 2216 94-95 -3.3491788455823226 2216 96-97 -3.5477846723506374 2216 98-99 -3.2995534144112995 2216 100 -2.965228489457459 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 150351.0 3 20208.0 4 13212.0 5 10824.0 6 10167.0 7 11429.0 8 13054.0 9 15344.0 10 16785.0 11 18365.0 12 20408.0 13 21870.0 14 24113.0 15 24360.0 16 24573.0 17 25395.0 18 24627.0 19 24058.0 20 24693.0 21 26991.0 22 30400.0 23 35875.0 24 43458.0 25 53520.0 26 64719.0 27 77842.0 28 93743.0 29 116010.0 30 140634.0 31 165911.0 32 206630.0 33 263028.0 34 346648.0 35 482934.0 36 755331.0 37 1222631.0 38 1422300.0 39 444099.0 40 849.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.74415302632322 20.72981220432904 13.974400441037856 23.551634328309888 2 15.620180367773115 20.380500567908992 40.27136273795356 23.72795632636433 3 18.377153370876698 25.158289210551597 30.462321223514188 26.00223619505752 4 12.757925129372166 16.65473395186071 38.994950325930425 31.5923905928367 5 13.037501757952732 37.788535557523915 36.01820005378718 13.15576263073617 6 30.936850416042137 38.09269813659999 17.606046026782135 13.364405420575737 7 26.82808846639164 32.492495419071496 22.79482050917504 17.88459560536182 8 24.70894223994777 36.53068062820766 20.76027751816369 18.000099613680874 9 24.6229871806852 16.828931984879702 21.88922597145623 36.65885486297887 10-11 23.75318483835755 27.034907971799022 29.244753642354084 19.967153547489346 12-13 23.98249336542806 24.632943560263193 29.194691725636357 22.18987134867239 14-15 21.421103164727604 27.020094878066285 26.232407546544863 25.32639441066124 16-17 21.053573391816787 30.292202941041772 27.072721758117996 21.58150190902344 18-19 20.958042512298984 29.29590932305005 28.966114260901314 20.779933903749647 20-21 21.96531613642586 28.60841654498425 28.49225060540519 20.9340167131847 22-23 21.65374992974238 28.461989142277215 28.4984344404444 21.385826487536008 24-25 21.53507206634389 28.525808491729563 28.48675056642087 21.452368875505677 26-27 21.29838237264656 28.545455302418304 28.52710594012032 21.629056384814806 28-29 21.29434056178975 28.534770389573062 28.69381709312987 21.477071955507324 30-31 21.576024841808945 28.89122395067165 28.243502586506253 21.28924862101315 32-33 21.287100507007914 28.635696838858358 28.55732614137303 21.5198765127607 34-35 21.455420927281843 28.259851029035467 28.68399986523209 21.600728178450595 36-37 21.631549382817894 28.473672175327735 28.304560592830345 21.590217849024025 38-39 21.379769097901637 28.506050409646612 28.55962503773893 21.554555454712826 40-41 21.28224815807691 28.152166597137345 28.887771656816014 21.67781358796973 42-43 21.054579310246993 28.151638218130355 28.90655574055576 21.887226731066885 44-45 21.05497410777285 28.231717546517192 28.896747805513478 21.81656054019648 46-47 21.062411430956562 28.343944207660694 28.790770478303866 21.80287388307888 48-49 20.88691107783464 28.281856012966976 28.77584277229619 22.055390136902194 50-51 20.874592146908757 28.190239774173453 28.735076996449603 22.20009108246818 52-53 20.820051374639355 28.098934806572583 28.868976166256587 22.21203765253147 54-55 20.886304361116924 28.366114033554574 28.690468379826488 22.057113225502018 56-57 20.943587336620595 28.107710794069362 28.5832605677856 22.365441301524445 58-59 20.88782955039397 27.730965713853095 28.724475894969448 22.65672884078349 60-61 21.05293184784628 27.92133579732369 28.402793036374696 22.622939318455334 62-63 20.984059073488528 27.84975082930333 28.41854262646476 22.747647470743384 64-65 21.068263029877844 27.606696399316945 28.432297830184677 22.89274274062053 66-67 20.94403378603412 27.689938509171203 28.233788501153338 23.13223920364134 68-69 21.197918112207923 27.752911898307907 28.163877350257344 22.885292639226822 70-71 21.068987782148714 27.50191873654427 28.315987286804372 23.11310619450264 72-73 20.87827030054123 27.47170408874106 28.45518031888042 23.19484529183729 74-75 20.95126521046855 27.412514436920958 28.43165832799004 23.204562024620458 76-77 21.060397892251785 27.358432453814853 28.3142416401389 23.266928013794466 78-79 21.17446692638022 27.313350896159783 28.50442723694173 23.007754940518264 80-81 21.13943820245509 27.172688231597164 28.563574748961834 23.124298816985913 82-83 21.044499339069876 27.190748612648076 28.661300794981837 23.103451253300218 84-85 21.152560441922436 26.970108004308702 28.5967411520069 23.280590401761966 86-87 21.46452762868381 26.961230257791648 28.471105409476877 23.103136704047664 88-89 21.522835399630093 26.682990423926846 28.561746436437957 23.232427740005104 90-91 21.731400040136243 26.560469842271356 28.493739910526866 23.214390207065534 92-93 21.902825056329515 26.606588020443763 28.316926731466612 23.17366019176011 94-95 21.859586115147085 26.2457044077437 28.433116277463306 23.461593199645915 96-97 21.986842472372167 26.134689934579225 28.329016496467226 23.549451096581382 98-99 22.094859734786983 26.130242845002822 28.24880703161164 23.526090388598554 100 22.195894218768135 26.089587043416078 28.30408350724768 23.41043523056811 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 784.0 1 701.5 2 772.5 3 978.5 4 957.0 5 828.0 6 678.0 7 546.0 8 544.5 9 559.5 10 532.0 11 542.0 12 609.0 13 682.0 14 763.5 15 917.5 16 1151.5 17 1515.0 18 2080.0 19 2941.0 20 4352.0 21 6417.5 22 9422.0 23 13799.0 24 20322.0 25 29152.5 26 40410.5 27 54047.0 28 70286.5 29 89371.5 30 108305.0 31 125170.0 32 140140.5 33 154675.0 34 169308.5 35 183417.5 36 197477.5 37 211066.0 38 222878.0 39 230338.0 40 236730.5 41 243035.5 42 245845.0 43 247094.0 44 246211.5 45 243725.0 46 239820.0 47 233013.5 48 225691.5 49 219298.5 50 212882.5 51 204054.5 52 190963.0 53 178502.5 54 168074.5 55 156655.5 56 141714.0 57 125095.5 58 110250.0 59 96384.0 60 82668.5 61 68648.5 62 56864.0 63 46739.0 64 37292.0 65 29802.0 66 23655.5 67 18390.5 68 14288.0 69 11048.5 70 8465.5 71 6534.5 72 4898.0 73 3472.5 74 2576.0 75 1922.0 76 1384.5 77 1017.0 78 743.5 79 533.0 80 396.5 81 304.5 82 244.5 83 188.5 84 149.5 85 119.0 86 93.5 87 84.5 88 66.0 89 48.5 90 46.5 91 35.5 92 31.5 93 34.5 94 37.0 95 40.0 96 43.5 97 43.0 98 48.0 99 65.0 100 137.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013426048599829609 2 0.0338348756333249 3 0.034929306690257046 4 0.03724148497955032 5 0.03966156492234395 6 0.038058454641767284 7 0.036224126532261286 8 0.03600832322526058 9 0.035931250615617465 10-11 0.036470758883119236 12-13 0.037287728545336185 14-15 0.035892714310795916 16-17 0.032925418839536216 18-19 0.03546881495775881 20-21 0.034983257517007225 22-23 0.03587729978886729 24-25 0.033133514885572604 26-27 0.03314122214653692 28-29 0.03371155945789593 30-31 0.03380404658946766 32-33 0.03367302315307437 34-35 0.03338014723643056 36-37 0.031229821427387814 38-39 0.031491868300174385 40-41 0.03260942113999946 42-43 0.03254005579132067 44-45 0.03152269734403163 46-47 0.030559289723492766 48-49 0.030713434942778985 50-51 0.031715378868139396 52-53 0.032185521786962365 54-55 0.0314995755611387 56-57 0.03002748871695531 58-59 0.030829043857243648 60-61 0.030428266287099482 62-63 0.030451388069992414 64-65 0.027961942778519988 66-67 0.028809741484594184 68-69 0.029734612800311498 70-71 0.03085216564013658 72-73 0.028100673475877583 74-75 0.028478329263128817 76-77 0.029125739184130934 78-79 0.029449444144631996 80-81 0.029611296624882523 82-83 0.02823940417323518 84-85 0.029395493317881816 86-87 0.02958046758102528 88-89 0.03011997584852704 90-91 0.030497631635778278 92-93 0.029133446445095246 94-95 0.030243292023956016 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6487389.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.62724751825248 #Duplication Level Percentage of deduplicated Percentage of total 1 77.91782918386203 49.57697003566507 2 13.286190508583765 16.90727464128632 3 4.062777933363307 7.755101315334044 4 1.7033277269583762 4.33512219551532 5 0.8666083870033904 2.756995317062913 6 0.525617900715808 2.0066172161321387 7 0.33064603063214676 1.4726667782371508 8 0.24055705610788172 1.2244786680990671 9 0.164594457929156 0.9425423083311905 >10 0.8419955494450917 9.426247744865222 >50 0.04400713728579691 1.908418922377425 >100 0.015654130150830867 1.5963327765437691 >500 1.6975034472338147E-4 0.07068186241108979 >1k 2.42476176449568E-5 0.02055021813938652 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.5414521928621824E-5 0.0 4 0.0 0.0 0.0 3.082904385724365E-5 0.0 5 0.0 0.0 0.0 7.707260964310912E-5 0.0 6 0.0 0.0 0.0 7.707260964310912E-5 0.0 7 0.0 0.0 0.0 9.248713157173094E-5 0.0 8 0.0 0.0 0.0 1.0790165350035276E-4 0.0 9 0.0 0.0 0.0 1.8497426314346188E-4 0.0 10-11 0.0 0.0 0.0 2.928759166438146E-4 0.0 12-13 0.0 0.0 0.0 4.0848483110847834E-4 0.0 14-15 0.0 0.0 0.0 4.2389935303710014E-4 0.0 16-17 0.0 0.0 0.0 5.24093745573142E-4 0.0 18-19 0.0 0.0 0.0 6.319953990734947E-4 0.0 20-21 0.0 1.5414521928621824E-5 0.0 8.940422718600658E-4 0.0 22-23 0.0 1.5414521928621824E-5 0.0 0.001988473328792215 0.0 24-25 0.0 1.5414521928621824E-5 0.0 0.004185042703620825 0.0 26-27 0.0 1.5414521928621824E-5 0.0 0.008501108843634935 0.0 28-29 0.0 1.5414521928621824E-5 0.0 0.029326127969203017 0.0 30-31 0.0 1.5414521928621824E-5 0.0 0.0961480805297786 0.0 32-33 0.0 2.3121782892932738E-5 0.0 0.24603118450273292 0.0 34-35 0.0 3.082904385724365E-5 0.0 0.5015577145134968 0.0 36-37 0.0 3.853630482155456E-5 0.0 0.9059576973108904 0.0 38-39 0.0 4.624356578586547E-5 0.0 1.4172342679003833 0.0 40-41 0.0 4.624356578586547E-5 0.0 1.9118477402850362 0.0 42-43 0.0 4.624356578586547E-5 0.0 2.381990659108002 0.0 44-45 0.0 4.624356578586547E-5 0.0 2.938100366726891 0.0 46-47 0.0 4.624356578586547E-5 0.0 3.583167280395857 0.0 48-49 0.0 4.624356578586547E-5 0.0 4.219594354523831 0.0 50-51 0.0 6.16580877144873E-5 0.0 4.811858823326303 0.0 52-53 0.0 8.477987060742003E-5 0.0 5.364284460204251 0.0 54-55 0.0 9.248713157173094E-5 0.0 5.958529695074551 0.0 56-57 0.0 9.248713157173094E-5 0.0 6.6225410561937945 0.0 58-59 0.0 9.248713157173094E-5 0.0 7.3068918789978525 0.0 60-61 1.5414521928621824E-5 9.248713157173094E-5 0.0 7.971381090296882 0.0 62-63 1.5414521928621824E-5 9.248713157173094E-5 0.0 8.589256787283759 0.0 64-65 1.5414521928621824E-5 1.0790165350035276E-4 0.0 9.216180808642736 0.0 66-67 1.5414521928621824E-5 1.0790165350035276E-4 0.0 9.89812696602593 0.0 68-69 1.5414521928621824E-5 1.0790165350035276E-4 0.0 10.60797340809993 0.0 70-71 1.5414521928621824E-5 1.0790165350035276E-4 0.0 11.3179123373055 0.0 72-73 1.5414521928621824E-5 1.0790165350035276E-4 0.0 12.001091656442984 0.0 74-75 1.5414521928621824E-5 1.0790165350035276E-4 0.0 12.664910336038119 0.0 76-77 1.5414521928621824E-5 1.0790165350035276E-4 0.0 13.355604234615805 0.0 78-79 1.5414521928621824E-5 1.0790165350035276E-4 0.0 14.085104192148798 1.5414521928621824E-5 80-81 1.5414521928621824E-5 1.0790165350035276E-4 0.0 14.825039781027467 1.5414521928621824E-5 82-83 1.5414521928621824E-5 1.0790165350035276E-4 0.0 15.543626257034996 1.5414521928621824E-5 84-85 1.5414521928621824E-5 1.1560891446466367E-4 0.0 16.227938543534233 1.5414521928621824E-5 86-87 1.5414521928621824E-5 1.233161754289746E-4 0.0 16.92647843377359 1.5414521928621824E-5 88 1.5414521928621824E-5 1.3873069735759641E-4 0.0 17.469478090492185 1.5414521928621824E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5850 0.0 42.592396 1 GTATCAA 9280 0.0 34.144756 1 TCAACGC 9725 0.0 31.033978 4 ATCAACG 9830 0.0 30.845951 3 CAACGCA 10395 0.0 29.214603 5 AACGCAG 10950 0.0 27.643738 6 TATCAAC 11415 0.0 26.851196 2 GTACATG 14930 0.0 24.435091 1 ACGCAGA 12575 0.0 23.659595 7 TACATGG 14990 0.0 23.081718 2 GCCGTAT 1555 0.0 22.970016 94 CGCAGAG 12940 0.0 22.810614 8 ACATGGG 15765 0.0 21.529562 3 GAGTACA 11685 0.0 19.995844 1 GTGGTAT 3870 0.0 19.558115 1 CATGGGG 10960 0.0 19.430305 4 GCAGAGT 14915 0.0 19.002277 9 GCCGGAT 1510 0.0 18.05216 94 CAGAGTA 14015 0.0 17.288095 10-11 AGTACAT 12130 0.0 17.129845 2 >>END_MODULE