FastQCFastQC Report
Wed 25 May 2016
SRR2049368_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049368_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6487389
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT175140.26996993705788264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99740.15374444171607407No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97940.15096982776892212No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT91400.14088873042760347TruSeq Adapter, Index 11 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92250.051.6229631
GTATCAA160950.038.642811
ATCAACG192500.031.7620963
TCAACGC193450.031.5818234
CAACGCA199500.030.6005215
TATCAAC206500.029.9728662
AACGCAG207250.029.4126856
GTGGTAT49700.028.8498291
TGGTATC46400.027.9546242
ACGCAGA238250.025.4277047
CGCAGAG241150.024.9853888
GTACATG153150.022.5002211
GCAGAGT267950.022.0299849
TACATGG151750.021.2140922
GAGTACT186650.019.6728112-13
AGAGTAC246650.019.62268610-11
ACATGGG156850.019.535553
CAGAGTA260950.019.520710-11
AGTACTT196500.018.11242912-13
GTACTTT206850.017.5511514-15
Produced by FastQC (version 0.11.5)