##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049368_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6487389
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.723288830067073	31.0	31.0	34.0	30.0	34.0
2	31.80032336584102	31.0	31.0	34.0	30.0	34.0
3	31.80424759483361	33.0	31.0	34.0	30.0	34.0
4	35.5763892068134	37.0	35.0	37.0	33.0	37.0
5	35.70193663429155	37.0	35.0	37.0	33.0	37.0
6	35.77625204839728	37.0	35.0	37.0	33.0	37.0
7	35.70996282171456	37.0	35.0	37.0	33.0	37.0
8	35.70725649409955	37.0	35.0	37.0	33.0	37.0
9	37.43300717746384	39.0	37.0	39.0	34.0	39.0
10-11	37.40223578391861	39.0	37.0	39.0	34.0	39.0
12-13	37.35955898436181	39.0	37.0	39.0	34.0	39.0
14-15	38.635787063177496	40.0	38.0	41.0	34.0	41.0
16-17	38.661848857837875	40.0	38.0	41.0	34.0	41.0
18-19	38.71900544271355	40.0	38.0	41.0	34.0	41.0
20-21	38.75097485290307	40.0	38.0	41.0	34.0	41.0
22-23	38.71973470374599	40.0	38.0	41.0	34.0	41.0
24-25	38.70315869759004	40.0	38.0	41.0	34.0	41.0
26-27	38.58601141383691	40.0	38.0	41.0	34.0	41.0
28-29	38.49893285264688	40.0	38.0	41.0	34.0	41.0
30-31	38.40178213453825	40.0	38.0	41.0	34.0	41.0
32-33	38.279057028952636	40.0	38.0	41.0	34.0	41.0
34-35	38.182546861302754	40.0	38.0	41.0	34.0	41.0
36-37	38.06902738528551	40.0	38.0	41.0	33.0	41.0
38-39	37.965755714664255	40.0	37.5	41.0	33.0	41.0
40-41	37.87916918501419	40.0	37.0	41.0	33.0	41.0
42-43	37.75879887887099	40.0	37.0	41.0	33.0	41.0
44-45	37.61141346695874	40.0	37.0	41.0	33.0	41.0
46-47	37.41957087820693	40.0	37.0	41.0	32.0	41.0
48-49	37.24459586129335	40.0	37.0	41.0	32.0	41.0
50-51	37.10293910231065	40.0	37.0	41.0	31.0	41.0
52-53	36.97777742324377	40.0	36.0	41.0	31.0	41.0
54-55	36.83168937456965	39.5	36.0	41.0	31.0	41.0
56-57	36.689527867066396	39.0	36.0	41.0	31.0	41.0
58-59	36.49217250576464	39.0	35.0	41.0	31.0	41.0
60-61	36.28966245125736	39.0	35.0	41.0	30.0	41.0
62-63	36.07631382980118	39.0	35.0	41.0	30.0	41.0
64-65	35.82247881235425	38.0	35.0	40.0	30.0	41.0
66-67	35.507563751765154	38.0	35.0	40.0	29.0	41.0
68-69	35.16464736429401	37.0	34.5	40.0	29.0	41.0
70-71	34.79272624163589	37.0	34.0	39.5	28.5	41.0
72-73	34.48033399877825	36.0	34.0	39.0	28.0	41.0
74-75	34.16075897406491	36.0	34.0	39.0	28.0	40.5
76-77	33.817696302780675	35.0	34.0	38.5	28.0	40.0
78-79	33.464361317010585	35.0	34.0	37.0	27.5	39.0
80-81	33.12866909938652	35.0	33.0	37.0	27.0	39.0
82-83	32.81488014669692	35.0	33.0	36.0	26.5	39.0
84-85	32.537988472712215	35.0	33.0	36.0	26.0	37.0
86-87	32.30366430932383	35.0	33.0	36.0	26.0	37.0
88-89	32.06237948734075	35.0	33.0	35.0	26.0	36.5
90-91	31.851099186436947	35.0	33.0	35.0	25.5	36.0
92-93	31.6611393273935	35.0	33.0	35.0	25.0	36.0
94-95	31.49622313383705	35.0	33.0	35.0	25.0	36.0
96-97	31.289432774880616	35.0	33.0	35.0	24.5	35.5
98-99	31.123849594960316	35.0	33.0	35.0	24.0	35.0
100	30.993152561068868	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.20490567922391634
1101	2	0.2127574633779794
1101	3	0.387168281797738
1101	4	0.22387012983382704
1101	5	0.20906318259967804
1101	6	0.183715304948457
1101	7	0.10361401774553514
1101	8	0.13826293695574776
1101	9	0.25610545242201965
1101	10-11	0.2540278946789698
1101	12-13	0.22869485174352633
1101	14-15	0.3131181871449087
1101	16-17	0.18540868963439294
1101	18-19	0.28729750501057083
1101	20-21	0.29039166232769276
1101	22-23	0.292800238260881
1101	24-25	0.28196547878931
1101	26-27	0.3932699981751995
1101	28-29	0.34813438663341145
1101	30-31	0.3019858935002091
1101	32-33	0.3193011510724091
1101	34-35	0.35251104120902
1101	36-37	0.3520844715991487
1101	38-39	0.38587622546248923
1101	40-41	0.4052581335154457
1101	42-43	0.41465549258501255
1101	44-45	0.41565642901448285
1101	46-47	0.45634570723900936
1101	48-49	0.4508070930698338
1101	50-51	0.3635714400155621
1101	52-53	0.43060118159259986
1101	54-55	0.44611251094676163
1101	56-57	0.4572747209193295
1101	58-59	0.44964377440866343
1101	60-61	0.47458580310333787
1101	62-63	0.4589196580039214
1101	64-65	0.3430810729650844
1101	66-67	0.3669693439440991
1101	68-69	0.37500802722252047
1101	70-71	0.3975056964384791
1101	72-73	0.34923312750715496
1101	74-75	0.43781066431427007
1101	76-77	0.35267323692761465
1101	78-79	0.42674967272678543
1101	80-81	0.42979164301148387
1101	82-83	0.40430958667894146
1101	84-85	0.4272552410695667
1101	86-87	0.3271628760033636
1101	88-89	0.4239905062070406
1101	90-91	0.4781384779115143
1101	92-93	0.5310687701617525
1101	94-95	0.5261046010156782
1101	96-97	0.57617928546874
1101	98-99	0.6351190654052807
1101	100	0.6916985479228295
1102	1	0.23084143485776565
1102	2	0.22313817995614116
1102	3	0.4137445769190542
1102	4	0.2545576800322067
1102	5	0.20237121833979188
1102	6	0.21545076713324107
1102	7	0.1888044112505156
1102	8	0.2195410452179729
1102	9	0.1708910743737988
1102	10-11	0.24594341827526023
1102	12-13	0.2315421012685448
1102	14-15	0.32193011084613943
1102	16-17	0.278154233125143
1102	18-19	0.2971186598053208
1102	20-21	0.2543746007272816
1102	22-23	0.28815902791053105
1102	24-25	0.23430059480180176
1102	26-27	0.35235487025470036
1102	28-29	0.3312156810290219
1102	30-31	0.27560750544765256
1102	32-33	0.27196564894273223
1102	34-35	0.32332363174847245
1102	36-37	0.39323464049711987
1102	38-39	0.35978935588683925
1102	40-41	0.39220058721345197
1102	42-43	0.38558673555284173
1102	44-45	0.46150664396981966
1102	46-47	0.3878336771102937
1102	48-49	0.35058129626607837
1102	50-51	0.4099736013159543
1102	52-53	0.45887324806412266
1102	54-55	0.36199910769980903
1102	56-57	0.47796006257769363
1102	58-59	0.3881754147627632
1102	60-61	0.4283261404263854
1102	62-63	0.4609527662321966
1102	64-65	0.39294656709321174
1102	66-67	0.40866973612345703
1102	68-69	0.38027397392527007
1102	70-71	0.3845159910521758
1102	72-73	0.38602982511098816
1102	74-75	0.32594912543478216
1102	76-77	0.33990999493175167
1102	78-79	0.4243358051581865
1102	80-81	0.3690310915103652
1102	82-83	0.4541350730293843
1102	84-85	0.42587945018962614
1102	86-87	0.4148056796216437
1102	88-89	0.4397199205641087
1102	90-91	0.4613751058683633
1102	92-93	0.48618681637356786
1102	94-95	0.4552421862664495
1102	96-97	0.5271532000455323
1102	98-99	0.5208705236579654
1102	100	0.5252458052708135
1103	1	0.20960613358512248
1103	2	0.20030222222507277
1103	3	0.36805353280127306
1103	4	0.1843070093893715
1103	5	0.2092810088345871
1103	6	0.1563718844519144
1103	7	0.16502547372657972
1103	8	0.17643591987339136
1103	9	0.21865410455043843
1103	10-11	0.25625730206013486
1103	12-13	0.2610352764349315
1103	14-15	0.3486971676107231
1103	16-17	0.3104481949435396
1103	18-19	0.3140945699054427
1103	20-21	0.391429981783169
1103	22-23	0.36945020409321216
1103	24-25	0.3664076859215015
1103	26-27	0.3571546429811008
1103	28-29	0.39249379510648197
1103	30-31	0.31583413620295175
1103	32-33	0.3058024241121373
1103	34-35	0.3154611071525295
1103	36-37	0.350712660116308
1103	38-39	0.3848653611244117
1103	40-41	0.37051280596225666
1103	42-43	0.44326724938724027
1103	44-45	0.4669812094121255
1103	46-47	0.4099897660855447
1103	48-49	0.4127950818263173
1103	50-51	0.3921298605611696
1103	52-53	0.3891587497210125
1103	54-55	0.3996725378759862
1103	56-57	0.3804898373810559
1103	58-59	0.41712842210327494
1103	60-61	0.39823665227691407
1103	62-63	0.3503772093247193
1103	64-65	0.3858578803287358
1103	66-67	0.3776469156253839
1103	68-69	0.3697907463247603
1103	70-71	0.4428305646085491
1103	72-73	0.41313453055129656
1103	74-75	0.3919773086057603
1103	76-77	0.3246724058671404
1103	78-79	0.3275456921297959
1103	80-81	0.41006399925743153
1103	82-83	0.39857013389128326
1103	84-85	0.3682471201445878
1103	86-87	0.4291656243544324
1103	88-89	0.46814579748651397
1103	90-91	0.4728191476756507
1103	92-93	0.5030696241837944
1103	94-95	0.5059598160096748
1103	96-97	0.5820149978237978
1103	98-99	0.63911692677015
1103	100	0.655062705921349
1104	1	0.1636112935195051
1104	2	0.2367262853192038
1104	3	0.2977502184005125
1104	4	0.1937954077850108
1104	5	0.12688533979102345
1104	6	0.17476100007750262
1104	7	0.1677959448956372
1104	8	0.18776014021245402
1104	9	0.20814850933694373
1104	10-11	0.2425331845641523
1104	12-13	0.19093510843605088
1104	14-15	0.23837887407836433
1104	16-17	0.2404821808667137
1104	18-19	0.2420377896015964
1104	20-21	0.24940711416206796
1104	22-23	0.24499250295666997
1104	24-25	0.22875856179754095
1104	26-27	0.2672962253977147
1104	28-29	0.3369020537733647
1104	30-31	0.3346057471282293
1104	32-33	0.34818667463549247
1104	34-35	0.3045787800973727
1104	36-37	0.29557036709855566
1104	38-39	0.33379397935039634
1104	40-41	0.2885099703859808
1104	42-43	0.35408711137456095
1104	44-45	0.3430794143648086
1104	46-47	0.4156368619680677
1104	48-49	0.34995353003659346
1104	50-51	0.32619446422686593
1104	52-53	0.3240133922112278
1104	54-55	0.2866680785369695
1104	56-57	0.31681735607445916
1104	58-59	0.37370003355363224
1104	60-61	0.3196879083575155
1104	62-63	0.33958389192075344
1104	64-65	0.2660076723208604
1104	66-67	0.32934535149048116
1104	68-69	0.27040227907973957
1104	70-71	0.31708788839464574
1104	72-73	0.2883484756903627
1104	74-75	0.30707845650518095
1104	76-77	0.2827669956048737
1104	78-79	0.3217664085489247
1104	80-81	0.33342849241009276
1104	82-83	0.37904167895538876
1104	84-85	0.37120477693687093
1104	86-87	0.3623904195102696
1104	88-89	0.39684696543353226
1104	90-91	0.3643327211037928
1104	92-93	0.41874307839365343
1104	94-95	0.40570330888811057
1104	96-97	0.4321472356636704
1104	98-99	0.4479641289121332
1104	100	0.4004200151348627
1105	1	0.20414150572739587
1105	2	0.08121442087578501
1105	3	0.2344471496040903
1105	4	0.1732700734480943
1105	5	0.1317420114543566
1105	6	0.1398338675604549
1105	7	0.12645218104499634
1105	8	0.18164718769217103
1105	9	0.21422990318328772
1105	10-11	0.22518359798331744
1105	12-13	0.19184865461824074
1105	14-15	0.24305655305974483
1105	16-17	0.24805976557042442
1105	18-19	0.2294891957850922
1105	20-21	0.22876427582854575
1105	22-23	0.21991522238565153
1105	24-25	0.2183054212112765
1105	26-27	0.2370550860507592
1105	28-29	0.27550015211708967
1105	30-31	0.23780208865451158
1105	32-33	0.3110944355306984
1105	34-35	0.3253933542197984
1105	36-37	0.33137066030693774
1105	38-39	0.3403100552160865
1105	40-41	0.34597778697311554
1105	42-43	0.33474827104016214
1105	44-45	0.39316740325846666
1105	46-47	0.37006924995482393
1105	48-49	0.3143563390554718
1105	50-51	0.2656921823531917
1105	52-53	0.30587789319175585
1105	54-55	0.26586503854917964
1105	56-57	0.24047486797387307
1105	58-59	0.3177693326423139
1105	60-61	0.2912429541660302
1105	62-63	0.305480862643698
1105	64-65	0.29942469292081597
1105	66-67	0.30417809932942674
1105	68-69	0.3112795672825115
1105	70-71	0.37804203601232444
1105	72-73	0.36647557743263803
1105	74-75	0.3587391193165814
1105	76-77	0.34808666900559615
1105	78-79	0.3437482798155287
1105	80-81	0.33586495019489604
1105	82-83	0.33795129227533494
1105	84-85	0.34212909247649037
1105	86-87	0.33941657528582425
1105	88-89	0.39933048355563017
1105	90-91	0.39624561044904993
1105	92-93	0.4465367544689869
1105	94-95	0.4887080199520035
1105	96-97	0.5849861071242088
1105	98-99	0.5614627210481835
1105	100	0.5870022858264115
1106	1	0.15561501725956006
1106	2	0.16486980579255928
1106	3	0.2917596623899321
1106	4	0.2241697827061273
1106	5	0.1604789823665982
1106	6	0.14898884609176122
1106	7	0.17291195408765958
1106	8	0.17086511429229034
1106	9	0.21319901380462625
1106	10-11	0.1757733534081396
1106	12-13	0.19136518513794698
1106	14-15	0.29798657337609313
1106	16-17	0.27748156030281024
1106	18-19	0.26371785485538624
1106	20-21	0.26022414302786245
1106	22-23	0.24166850012997543
1106	24-25	0.23446561369689078
1106	26-27	0.26052776406770306
1106	28-29	0.28728351545799313
1106	30-31	0.31523253082454517
1106	32-33	0.19286489678733432
1106	34-35	0.24525689813825124
1106	36-37	0.2600697649198409
1106	38-39	0.28286907079185397
1106	40-41	0.2579127440704454
1106	42-43	0.3085593766648387
1106	44-45	0.2500652760554942
1106	46-47	0.3547166500479051
1106	48-49	0.31828786019084276
1106	50-51	0.3112713361295363
1106	52-53	0.3150679426342293
1106	54-55	0.3032435591094256
1106	56-57	0.2619400808645338
1106	58-59	0.34693396811870514
1106	60-61	0.25905929946710415
1106	62-63	0.269638463419831
1106	64-65	0.24340862110496886
1106	66-67	0.26160345302908183
1106	68-69	0.29457435638173735
1106	70-71	0.31289745340414754
1106	72-73	0.3454843000824823
1106	74-75	0.28902163442995743
1106	76-77	0.27326428902918565
1106	78-79	0.3013461237752182
1106	80-81	0.30012250306786825
1106	82-83	0.30949318138817716
1106	84-85	0.3029349066205924
1106	86-87	0.3177248104798238
1106	88-89	0.3289569155119878
1106	90-91	0.33577329598684
1106	92-93	0.42956020167688536
1106	94-95	0.38649883229272675
1106	96-97	0.38425271266327243
1106	98-99	0.4139037546848172
1106	100	0.4012557458931987
1107	1	0.16506336331407212
1107	2	0.13488100765891886
1107	3	0.2313096708621707
1107	4	0.15670994807582872
1107	5	0.14814303273960405
1107	6	0.1534626793879852
1107	7	0.08977076897179614
1107	8	0.12408754349782924
1107	9	0.14598726458535793
1107	10-11	0.16301216388543338
1107	12-13	0.1548996755526133
1107	14-15	0.1967477423794719
1107	16-17	0.1756309218874037
1107	18-19	0.15256863738696325
1107	20-21	0.16568318229398926
1107	22-23	0.1188517789909298
1107	24-25	0.16157017771500648
1107	26-27	0.24704226653907568
1107	28-29	0.2134749195695207
1107	30-31	0.16137283641495515
1107	32-33	0.203374757042738
1107	34-35	0.16377819386768522
1107	36-37	0.18446688561016344
1107	38-39	0.22000700696170128
1107	40-41	0.23078945216369107
1107	42-43	0.30912639325006097
1107	44-45	0.2701894704516974
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2216	96-97	-1.5353827837890606
2216	98-99	-1.7600776266864493
2216	100	-1.6948683199312065
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	261.0
3	24.0
4	45.0
5	75.0
6	255.0
7	742.0
8	1696.0
9	3176.0
10	4836.0
11	7237.0
12	10086.0
13	13536.0
14	16676.0
15	20744.0
16	25118.0
17	33047.0
18	32632.0
19	20531.0
20	18246.0
21	19140.0
22	21454.0
23	25007.0
24	29338.0
25	36202.0
26	44036.0
27	49778.0
28	63368.0
29	83401.0
30	108575.0
31	140075.0
32	180242.0
33	234307.0
34	317459.0
35	448699.0
36	719054.0
37	1288386.0
38	1775213.0
39	692163.0
40	2529.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.67893284779314	21.27447207064511	14.30918770088208	23.73740738067967
2	15.648175252015871	20.76764627494975	39.64585752449869	23.93832094853569
3	17.782100009726566	25.44077440091846	31.105287504726476	25.6718380846285
4	12.587009222665458	17.424426814679006	38.20866552280602	31.779898439849514
5	12.45160418158985	37.98190612586975	36.19394489832504	13.37254479421536
6	30.57229958000052	38.50527538891224	17.1464051253902	13.776019905697037
7	26.2748079389104	32.975346476063024	22.533811368487385	18.216034216539196
8	24.590992785195198	36.75934182729325	20.421913090928427	18.227752296583123
9	24.27275441629907	16.50429779993153	21.73819081914157	37.484756964627834
10-11	23.608249790478112	27.11203999020253	29.02402646118492	20.255683758134435
12-13	24.097752373033167	24.492137284317376	29.291630786388772	22.118479556260688
14-15	21.375223366280622	26.774812491786943	26.525235717178692	25.324728424753744
16-17	20.67486015366584	30.22556765330148	27.416301644336542	21.683270548696136
18-19	20.54557559152861	28.584220426498376	30.033386323337886	20.83681765863513
20-21	21.446974731800577	27.810510971025533	29.828574185571117	20.913940111602773
22-23	21.126295956662997	27.836468570021005	29.895825577901984	21.14140989541401
24-25	21.158956222777437	27.95007168147452	29.839092989843895	21.05187910590415
26-27	20.94235035053798	27.914511940133664	29.92342600882898	21.219711700499378
28-29	20.776546649231744	27.74972666089442	30.195234951397047	21.27849173847679
30-31	21.21780590140393	28.009830149681957	29.900305801016852	20.872058147897263
32-33	20.815499335247306	27.9834910113875	30.05660224667142	21.144407406693773
34-35	20.943911696952235	27.685902481685037	30.255912762404396	21.114273058958332
36-37	21.324174328458334	27.79569459871819	29.668536757331843	21.211594315491634
38-39	21.06080820178917	27.68929295638522	29.949663692423655	21.300235149401953
40-41	20.919204529532028	27.385481136378452	30.22298983210636	21.47232450198316
42-43	20.918034045323886	27.342997574356996	30.19070030422274	21.54826807609638
44-45	20.75772485852506	27.600160804813296	30.017928688491235	21.624185648170414
46-47	20.972777766559418	27.621524001750476	29.66102191315725	21.744676318532857
48-49	20.807141915748144	27.630012940598874	29.47853008978263	22.08431505387035
50-51	20.664005541240957	27.609855818753882	29.476964843378784	22.249173796626376
52-53	20.581351757714174	27.65307261243658	29.64810262933409	22.117473000515155
54-55	20.59993293109977	27.94072502698903	29.273139444968244	22.186202596942962
56-57	20.695215860030412	27.738590414085746	29.025178532011214	22.54101519387263
58-59	20.436481358650386	27.600291273255344	29.28367527163127	22.67955209646299
60-61	20.684885696054696	28.057490746063756	28.62843049988867	22.629193057992875
62-63	20.345518615890796	28.146489756441046	28.608722198941766	22.89926942872639
64-65	20.381594479601713	27.931663398258582	28.59901519231712	23.087726929822587
66-67	20.468553269101047	27.98232481739673	28.340024367655637	23.209097545846586
68-69	20.818353727691484	28.036588453503526	28.0825706564025	23.062487162402498
70-71	20.727259078348997	27.79946011920415	28.091058023929	23.38222277851785
72-73	20.56247778356343	27.74914240315792	28.161030898149836	23.52734891512882
74-75	20.65886807239288	27.70497938826231	28.120150638406628	23.516001900938182
76-77	20.718712580942316	27.730658624742112	27.9521748218375	23.59845397247807
78-79	20.806403279331406	27.72072162062506	27.971894087658484	23.50098101238505
80-81	20.664160710074132	27.519981625382865	28.03191202381301	23.78394564072999
82-83	20.541085014047063	27.406639296773534	28.237123665575126	23.815152023604277
84-85	20.614452103636797	27.28017691470303	28.1280319349167	23.977339046743477
86-87	20.73458282833927	27.45552744502228	28.01397348275377	23.795916243884673
88-89	20.677682625393945	27.13180690446113	28.09817095212074	24.092339518024186
90-91	20.57708912888474	26.86857518013755	28.167480076714195	24.386855614263514
92-93	20.58164835977083	26.875231657450726	28.180143770400214	24.362976212378232
94-95	20.42690507501542	26.600818882547962	28.500841589034785	24.471434453401834
96-97	20.48589420338457	26.63439632067803	28.441847593562898	24.437861882374502
98-99	20.53382305766737	26.64930886068183	28.35367625443817	24.463191827212626
100	20.57165811868844	26.626407518439095	28.321133093192486	24.48080126967998
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	244.0
1	209.5
2	286.5
3	417.0
4	294.5
5	214.0
6	554.0
7	1351.5
8	2224.0
9	2644.5
10	2242.0
11	1733.0
12	1867.5
13	2205.5
14	2598.0
15	3091.0
16	3618.5
17	4175.0
18	4922.0
19	5863.0
20	7253.5
21	9293.0
22	12186.5
23	16482.0
24	22513.5
25	30719.0
26	41704.0
27	54737.0
28	70669.5
29	89352.0
30	107492.0
31	123804.0
32	138806.5
33	153315.5
34	166789.0
35	180154.5
36	193795.5
37	207361.5
38	219184.5
39	226950.0
40	234446.0
41	242036.5
42	247471.5
43	251353.0
44	252449.0
45	250518.5
46	246499.0
47	239530.5
48	230838.5
49	223330.5
50	216515.0
51	206825.0
52	192093.5
53	177838.5
54	165838.5
55	153663.0
56	138683.5
57	121475.5
58	106794.0
59	93957.5
60	80280.0
61	65834.0
62	53624.0
63	43634.5
64	34455.0
65	26769.5
66	20600.0
67	15680.5
68	11705.5
69	8686.0
70	6495.0
71	4721.5
72	3316.5
73	2173.5
74	1507.0
75	1038.0
76	644.0
77	380.0
78	222.0
79	121.5
80	58.5
81	28.0
82	16.0
83	13.5
84	10.5
85	8.0
86	4.0
87	1.0
88	0.5
89	0.5
90	0.5
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	1.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034543943642041504
2	0.0
3	0.0
4	3.082904385724365E-5
5	0.0
6	0.0
7	0.0
8	5.24093745573142E-4
9	0.0
10-11	0.0
12-13	0.044100947237787035
14-15	1.0019439253604185E-4
16-17	7.707260964310912E-6
18-19	3.082904385724365E-5
20-21	8.477987060742003E-5
22-23	0.0
24-25	1.310234363932855E-4
26-27	5.395082675017638E-5
28-29	4.007775701441674E-4
30-31	7.707260964310912E-6
32-33	2.1580330700070552E-4
34-35	3.853630482155456E-5
36-37	4.624356578586547E-5
38-39	3.6994852628692375E-4
40-41	1.233161754289746E-4
42-43	5.703373113590074E-4
44-45	3.2370496050105826E-4
46-47	1.233161754289746E-4
48-49	8.323841841455785E-4
50-51	5.16386484608831E-4
52-53	6.628244429307383E-4
54-55	4.7014291882296563E-4
56-57	5.703373113590074E-4
58-59	1.5414521928621823E-4
60-61	2.2351056796501641E-4
62-63	0.0010558947521105949
64-65	4.778501797872766E-4
66-67	0.001556866714790804
68-69	0.001487501366112006
70-71	4.778501797872766E-4
72-73	0.0011560891446466368
74-75	0.0010173584472890403
76-77	8.169696622169566E-4
78-79	0.0032832931707964482
80-81	0.002759199425223306
82-83	0.001395014234540275
84-85	0.0030058317760812557
86-87	0.0015645739757551153
88-89	0.002065545938435324
90-91	0.002982709993188323
92-93	0.002628175988830021
94-95	0.003946117613727187
96-97	0.0033218294756180025
98-99	0.003113733429581608
100	0.001310234363932855
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6487389.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.27314696563617
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.05420909534061	45.840265221586876
2	14.160740350333203	17.07024768555695
3	4.4486461879384285	8.044017164511901
4	1.9342724903242368	4.663387603235993
5	0.9940661369266092	2.9957747182269863
6	0.5751286447100878	2.0798887996054964
7	0.3754881603085729	1.5842297149094478
8	0.2478435443646389	1.195064829917924
9	0.19372360781363762	1.0508698336018178
>10	0.9438492160953696	10.025169271286535
>50	0.05134288119825104	2.1143080192162893
>100	0.019794555082715565	1.9971414043731026
>500	5.11513712645108E-4	0.21086348222388646
>1k	2.55743900874497E-4	0.3316889895756748
>5k	1.0229756016962724E-4	0.5271138896384276
>10k+	2.557439004240681E-5	0.26996937253267694
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT	17514	0.26996993705788264	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9974	0.15374444171607407	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9794	0.15096982776892212	No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	9140	0.14088873042760347	TruSeq Adapter, Index 11 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.5414521928621824E-5	0.0	1.5414521928621824E-5	0.0
4	0.0	1.5414521928621824E-5	0.0	3.082904385724365E-5	0.0
5	0.0	1.5414521928621824E-5	0.0	7.707260964310912E-5	0.0
6	0.0	1.5414521928621824E-5	0.0	1.0790165350035276E-4	0.0
7	0.0	1.5414521928621824E-5	0.0	1.0790165350035276E-4	0.0
8	0.0	1.5414521928621824E-5	0.0	1.0790165350035276E-4	0.0
9	0.0	1.5414521928621824E-5	0.0	1.8497426314346188E-4	0.0
10-11	0.0	1.5414521928621824E-5	0.0	3.237049605010583E-4	0.0
12-13	0.0	2.3121782892932738E-5	0.0	4.778501797872765E-4	0.0
14-15	0.0	3.082904385724365E-5	0.0	4.932647017158984E-4	0.0
16-17	0.0	3.082904385724365E-5	0.0	5.703373113590074E-4	0.0
18-19	0.0	4.624356578586547E-5	0.0	6.782389648593602E-4	0.0
20-21	0.0	4.624356578586547E-5	0.0	9.402858376459313E-4	0.0
22-23	0.0	4.624356578586547E-5	0.0	0.0020655459384353245	0.0
24-25	0.0	4.624356578586547E-5	0.0	0.004246700791335312	0.0
26-27	0.0	4.624356578586547E-5	0.0	0.008585888714242356	0.0
28-29	0.0	4.624356578586547E-5	0.0	0.02958817484198959	0.0
30-31	0.0	4.624356578586547E-5	0.0	0.0973349987182825	0.0
32-33	0.0	4.624356578586547E-5	0.0	0.24852062979420533	0.0
34-35	0.0	4.624356578586547E-5	0.0	0.5060356331337615	0.0
36-37	0.0	4.624356578586547E-5	0.0	0.9134029114024147	0.0
38-39	0.0	4.624356578586547E-5	0.0	1.428656428649492	0.0
40-41	0.0	4.624356578586547E-5	0.0	1.9272237259088365	0.0
42-43	0.0	4.624356578586547E-5	0.0	2.4013975422161367	0.0
44-45	0.0	4.624356578586547E-5	0.0	2.962940868814865	0.0
46-47	0.0	4.624356578586547E-5	0.0	3.613595546682957	0.0
48-49	0.0	4.624356578586547E-5	0.0	4.255941797231522	0.0
50-51	1.5414521928621824E-5	6.16580877144873E-5	0.0	4.854125442454584	0.0
52-53	1.5414521928621824E-5	6.16580877144873E-5	0.0	5.41144519004487	0.0
54-55	1.5414521928621824E-5	6.16580877144873E-5	0.0	6.01105467854633	0.0
56-57	1.5414521928621824E-5	6.16580877144873E-5	0.0	6.680476536862519	0.0
58-59	1.5414521928621824E-5	6.16580877144873E-5	0.0	7.371047119264777	0.0
60-61	1.5414521928621824E-5	6.16580877144873E-5	0.0	8.041956478947078	0.0
62-63	1.5414521928621824E-5	6.16580877144873E-5	0.0	8.666737881757976	0.0
64-65	1.5414521928621824E-5	6.16580877144873E-5	0.0	9.300120587805047	0.0
66-67	1.5414521928621824E-5	6.16580877144873E-5	0.0	9.989881907806053	0.0
68-69	1.5414521928621824E-5	6.16580877144873E-5	0.0	10.707165856710613	0.0
70-71	1.5414521928621824E-5	6.16580877144873E-5	0.0	11.425066386492315	0.0
72-73	1.5414521928621824E-5	6.16580877144873E-5	0.0	12.11768710031108	0.0
74-75	1.5414521928621824E-5	6.16580877144873E-5	0.0	12.790530982495422	0.0
76-77	1.5414521928621824E-5	6.16580877144873E-5	0.0	13.490697104798247	0.0
78-79	1.5414521928621824E-5	6.16580877144873E-5	0.0	14.230386061326058	0.0
80-81	1.5414521928621824E-5	6.16580877144873E-5	0.0	14.981050157467049	0.0
82-83	1.5414521928621824E-5	6.16580877144873E-5	0.0	15.709787096164574	0.0
84-85	1.5414521928621824E-5	6.16580877144873E-5	0.0	16.40353307008413	0.0
86-87	1.5414521928621824E-5	6.16580877144873E-5	0.0	17.111044212085943	0.0
88	1.5414521928621824E-5	6.16580877144873E-5	0.0	17.663084485915675	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9225	0.0	51.622963	1
GTATCAA	16095	0.0	38.64281	1
ATCAACG	19250	0.0	31.762096	3
TCAACGC	19345	0.0	31.581823	4
CAACGCA	19950	0.0	30.600521	5
TATCAAC	20650	0.0	29.972866	2
AACGCAG	20725	0.0	29.412685	6
GTGGTAT	4970	0.0	28.849829	1
TGGTATC	4640	0.0	27.954624	2
ACGCAGA	23825	0.0	25.427704	7
CGCAGAG	24115	0.0	24.985388	8
GTACATG	15315	0.0	22.500221	1
GCAGAGT	26795	0.0	22.029984	9
TACATGG	15175	0.0	21.214092	2
GAGTACT	18665	0.0	19.67281	12-13
AGAGTAC	24665	0.0	19.622686	10-11
ACATGGG	15685	0.0	19.53555	3
CAGAGTA	26095	0.0	19.5207	10-11
AGTACTT	19650	0.0	18.112429	12-13
GTACTTT	20685	0.0	17.55115	14-15
>>END_MODULE