FastQCFastQC Report
Wed 25 May 2016
SRR2049367_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049367_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5866697
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58500.046.614011
GTATCAA98000.035.501771
TCAACGC102550.032.731244
ATCAACG104200.032.1678243
CAACGCA108650.030.8073165
AACGCAG115900.028.7939156
TATCAAC122500.027.7077352
ACGCAGA132500.024.760847
GTACATG147500.024.0338691
CGCAGAG133650.023.9495078
TACATGG147200.022.9945342
ACATGGG149550.021.6587163
GCAGAGT156100.019.8127659
CATGGGG106900.018.4701944
GTGGTAT37600.018.2561571
CAGAGTA145900.018.12130210-11
AGAGTAC130200.017.16570710-11
GAGTACT69350.016.30011712-13
GAGTACA108850.016.2406641
TGGTATC38400.016.1600482
Produced by FastQC (version 0.11.5)