##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049367_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5866697 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.783382881372603 31.0 31.0 34.0 28.0 34.0 2 31.051505301875995 31.0 31.0 34.0 28.0 34.0 3 31.123108283928758 31.0 31.0 34.0 28.0 34.0 4 34.73224763440144 37.0 35.0 37.0 32.0 37.0 5 34.69501970188677 37.0 35.0 37.0 32.0 37.0 6 34.76329832612797 37.0 35.0 37.0 32.0 37.0 7 34.70424959734583 37.0 35.0 37.0 32.0 37.0 8 34.68143011306021 37.0 35.0 37.0 32.0 37.0 9 36.25463356979234 39.0 37.0 39.0 32.0 39.0 10-11 36.219611733825694 39.0 37.0 39.0 32.0 39.0 12-13 36.1533382412625 39.0 37.0 39.0 32.0 39.0 14-15 37.36675100145789 40.0 38.0 41.0 32.0 41.0 16-17 37.336883513840924 40.0 38.0 41.0 32.0 41.0 18-19 37.37926034018801 40.0 38.0 41.0 32.0 41.0 20-21 37.451209684086294 40.0 38.0 41.0 32.0 41.0 22-23 37.40837024649475 40.0 38.0 41.0 32.0 41.0 24-25 37.416136200659416 40.0 38.0 41.0 32.0 41.0 26-27 37.398148736844604 40.0 38.0 41.0 32.0 41.0 28-29 37.33190746002393 40.0 38.0 41.0 32.0 41.0 30-31 37.26448536544498 40.0 37.5 41.0 31.5 41.0 32-33 37.19716162263025 40.0 37.0 41.0 31.0 41.0 34-35 37.08083185478984 40.0 37.0 41.0 31.0 41.0 36-37 36.99936088398634 40.0 37.0 41.0 31.0 41.0 38-39 36.894482022848635 40.0 37.0 41.0 30.0 41.0 40-41 36.79139403313312 40.0 37.0 41.0 30.0 41.0 42-43 36.66389844234328 40.0 37.0 41.0 30.0 41.0 44-45 36.56406381648822 40.0 37.0 41.0 30.0 41.0 46-47 36.4547077341816 40.0 36.0 41.0 30.0 41.0 48-49 36.33490130818073 40.0 36.0 41.0 30.0 41.0 50-51 36.204598004635315 40.0 36.0 41.0 29.5 41.0 52-53 36.069247653321796 39.5 35.5 41.0 29.0 41.0 54-55 35.93980914644135 39.0 35.0 41.0 29.0 41.0 56-57 35.81947260954503 39.0 35.0 41.0 28.5 41.0 58-59 35.6240516597329 39.0 35.0 41.0 28.0 41.0 60-61 35.4183203939116 39.0 35.0 41.0 28.0 41.0 62-63 35.21673055554088 38.5 35.0 40.0 28.0 41.0 64-65 34.97328377449867 38.0 34.5 40.0 27.5 41.0 66-67 34.708338354614185 37.5 34.0 40.0 27.0 41.0 68-69 34.4130062452518 37.0 34.0 40.0 26.0 41.0 70-71 34.10708904857367 37.0 34.0 39.5 26.0 41.0 72-73 33.730678864103595 36.0 34.0 39.0 26.0 41.0 74-75 33.332327372625514 36.0 33.5 39.0 25.5 40.5 76-77 32.92540768681253 35.0 33.0 38.5 24.5 40.0 78-79 32.51022398122828 35.0 33.0 37.0 23.5 39.0 80-81 32.04684390552299 35.0 33.0 37.0 21.0 39.0 82-83 31.625098245230664 35.0 32.5 36.5 19.0 39.0 84-85 31.225244289930092 35.0 32.0 36.0 15.0 37.5 86-87 30.78799228935805 35.0 32.0 36.0 4.5 37.0 88-89 30.408837289534468 35.0 32.0 35.0 2.0 37.0 90-91 30.077044715280167 35.0 31.5 35.0 2.0 36.0 92-93 29.72529278399754 35.0 31.0 35.0 2.0 36.0 94-95 29.42342369138887 35.0 31.0 35.0 2.0 36.0 96-97 29.067758314431444 34.5 31.0 35.0 2.0 35.5 98-99 28.770125506737436 34.0 31.0 35.0 2.0 35.0 100 28.607201462765165 34.0 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3859924168686284 1101 2 0.43627593525409125 1101 3 0.4213608684932808 1101 4 0.4507375704505563 1101 5 0.4293271212001457 1101 6 0.5019029219294637 1101 7 0.4934455242235458 1101 8 0.5107583338949198 1101 9 0.610170601626784 1101 10-11 0.5765784969312051 1101 12-13 0.5857985121602951 1101 14-15 0.7060702897131108 1101 16-17 0.7125309352235689 1101 18-19 0.6721826488617211 1101 20-21 0.6477975413202444 1101 22-23 0.6530240281840491 1101 24-25 0.6512840356703578 1101 26-27 0.6117594473501171 1101 28-29 0.6437462761885726 1101 30-31 0.6936865686700742 1101 32-33 0.6089246315353378 1101 34-35 0.6357358956260981 1101 36-37 0.6301297908753085 1101 38-39 0.6724386923680186 1101 40-41 0.7100270053858324 1101 42-43 0.6231167553895105 1101 44-45 0.6018209515973467 1101 46-47 0.6164783683993136 1101 48-49 0.5576476341902463 1101 50-51 0.5684039081066032 1101 52-53 0.5808494778470248 1101 54-55 0.5796251314087328 1101 56-57 0.5305538561884902 1101 58-59 0.6335630603488056 1101 60-61 0.6695900951673721 1101 62-63 0.6517974309927297 1101 64-65 0.5955523206464974 1101 66-67 0.6027644595017989 1101 68-69 0.6101860578801848 1101 70-71 0.6275085229354147 1101 72-73 0.6910888087266471 1101 74-75 0.6666282800712651 1101 76-77 0.6223937316538084 1101 78-79 0.5272958584452851 1101 80-81 0.5991844748149617 1101 82-83 0.6504470241093756 1101 84-85 0.6233580163023333 1101 86-87 0.6548825898918764 1101 88-89 0.6428719526301059 1101 90-91 0.7008465861495949 1101 92-93 0.6789699132791789 1101 94-95 0.7967578764111174 1101 96-97 0.7303589811618778 1101 98-99 0.7430303251754147 1101 100 0.7740467628895793 1102 1 0.24629717136567209 1102 2 0.3665938705413865 1102 3 0.37003732522601496 1102 4 0.3620734772834595 1102 5 0.29373366338866447 1102 6 0.46023149500981475 1102 7 0.4964950720603767 1102 8 0.49523878369094376 1102 9 0.631670591451666 1102 10-11 0.49098632306733947 1102 12-13 0.59382702048282 1102 14-15 0.6522250411851758 1102 16-17 0.6221973426660341 1102 18-19 0.6423072781419563 1102 20-21 0.600494086251679 1102 22-23 0.62842189231079 1102 24-25 0.6073920454775958 1102 26-27 0.6058217294206827 1102 28-29 0.5797074942639284 1102 30-31 0.6187472093548791 1102 32-33 0.7175794444485462 1102 34-35 0.6854302954233091 1102 36-37 0.6479557841397039 1102 38-39 0.6335012010472951 1102 40-41 0.6130240514794636 1102 42-43 0.6105898826416691 1102 44-45 0.5915759224843526 1102 46-47 0.5162670384321757 1102 48-49 0.4988835800744553 1102 50-51 0.5459010150901236 1102 52-53 0.5896925923575509 1102 54-55 0.5632782274152603 1102 56-57 0.5316069688518894 1102 58-59 0.5932592558351786 1102 60-61 0.5811981575498635 1102 62-63 0.5532784312554497 1102 64-65 0.5323458767086748 1102 66-67 0.5593187272356985 1102 68-69 0.6046067427860748 1102 70-71 0.6131142069599989 1102 72-73 0.53885711254825 1102 74-75 0.5870207261228444 1102 76-77 0.5989279698050112 1102 78-79 0.5866459806683721 1102 80-81 0.5854649778155618 1102 82-83 0.6160892981142752 1102 84-85 0.6105970929974625 1102 86-87 0.5752469223142285 1102 88-89 0.5762582241110223 1102 90-91 0.6091447510324457 1102 92-93 0.5899625076806778 1102 94-95 0.5803042922196902 1102 96-97 0.5287174438075688 1102 98-99 0.5817298245185327 1102 100 0.5893245083198373 1103 1 0.14482925568572114 1103 2 0.2582335917012806 1103 3 0.23626599433396578 1103 4 0.24755658496323463 1103 5 0.22800629768006786 1103 6 0.3162361613293285 1103 7 0.32322514620750553 1103 8 0.34215378274156905 1103 9 0.4183593490090516 1103 10-11 0.392178194614921 1103 12-13 0.4033390888541817 1103 14-15 0.4753633741290457 1103 16-17 0.4449360383238883 1103 18-19 0.4774021348249704 1103 20-21 0.36834464661569655 1103 22-23 0.42526534480892764 1103 24-25 0.4098171892782432 1103 26-27 0.41624914767531607 1103 28-29 0.3973309684726942 1103 30-31 0.3782723288057781 1103 32-33 0.4676664516454352 1103 34-35 0.37221650016500973 1103 36-37 0.3395031357256215 1103 38-39 0.3662044682157344 1103 40-41 0.3480091765783726 1103 42-43 0.38491704670035887 1103 44-45 0.28190745144788565 1103 46-47 0.2577468827725298 1103 48-49 0.25830414631519005 1103 50-51 0.18877371059323877 1103 52-53 0.2873052787253485 1103 54-55 0.25805509688930783 1103 56-57 0.19377024639985052 1103 58-59 0.26402963052427 1103 60-61 0.2236656956729064 1103 62-63 0.2286328059606575 1103 64-65 0.24517397949002628 1103 66-67 0.19791012978557632 1103 68-69 0.26657326840995665 1103 70-71 0.19687512853181488 1103 72-73 0.22388838738593364 1103 74-75 0.1693979441163478 1103 76-77 0.2144670433527267 1103 78-79 0.0884396826616296 1103 80-81 0.09437423439119641 1103 82-83 0.18830995923052996 1103 84-85 0.24953591099110994 1103 86-87 0.21936087230164958 1103 88-89 0.10886528830682352 1103 90-91 0.14256710245407334 1103 92-93 0.1366877819745902 1103 94-95 0.13265204517778173 1103 96-97 0.1477761249484466 1103 98-99 0.22245040620676804 1103 100 0.15249887433484588 1104 1 0.024001281417010745 1104 2 0.10909940961154874 1104 3 0.10549967899417467 1104 4 0.1572061510925309 1104 5 0.1872390615602697 1104 6 0.2674215718409698 1104 7 0.30536811098301797 1104 8 0.2945328741191986 1104 9 0.3409838303411732 1104 10-11 0.3498566665700906 1104 12-13 0.3122178385154939 1104 14-15 0.4334856745458069 1104 16-17 0.42767714901233234 1104 18-19 0.3858163280585032 1104 20-21 0.3558659943376483 1104 22-23 0.3401096934070793 1104 24-25 0.4094525576187138 1104 26-27 0.34668850278488605 1104 28-29 0.36987589885658423 1104 30-31 0.33991593916889684 1104 32-33 0.38378699716384546 1104 34-35 0.3528483927345576 1104 36-37 0.35074026268646463 1104 38-39 0.462234107887987 1104 40-41 0.4720851641218786 1104 42-43 0.44896710102360515 1104 44-45 0.43631203662678075 1104 46-47 0.34818336721502874 1104 48-49 0.3244960974202442 1104 50-51 0.36322446809798237 1104 52-53 0.40647618924344897 1104 54-55 0.4405935665362719 1104 56-57 0.40776181105506737 1104 58-59 0.4837297812238788 1104 60-61 0.4287380963408083 1104 62-63 0.4266537678021791 1104 64-65 0.4617349925535734 1104 66-67 0.5259476934433494 1104 68-69 0.5578385937376424 1104 70-71 0.492284179146516 1104 72-73 0.47364025592354864 1104 74-75 0.5310441584953196 1104 76-77 0.5311568822884425 1104 78-79 0.5213328539876727 1104 80-81 0.5131711457367558 1104 82-83 0.49044253016356976 1104 84-85 0.5437329345801629 1104 86-87 0.6314335272603842 1104 88-89 0.6086714523109364 1104 90-91 0.5723070814025171 1104 92-93 0.5059315493841758 1104 94-95 0.5332151792295967 1104 96-97 0.513521268597767 1104 98-99 0.5908180507473553 1104 100 0.6333124716584457 1105 1 0.06128374464301345 1105 2 0.11810707123782294 1105 3 0.14366584616821854 1105 4 0.1623021754255376 1105 5 0.13105098325249998 1105 6 0.2079690062092041 1105 7 0.168562659398674 1105 8 0.27084256179729493 1105 9 0.29990938536187883 1105 10-11 0.31852355049102954 1105 12-13 0.2870502104092054 1105 14-15 0.3341201590815501 1105 16-17 0.2822357606422301 1105 18-19 0.2700002171631226 1105 20-21 0.3241367968151394 1105 22-23 0.28991203377506025 1105 24-25 0.23939415943219444 1105 26-27 0.22908072017754222 1105 28-29 0.26123611140787517 1105 30-31 0.23356311412439368 1105 32-33 0.30787856049389006 1105 34-35 0.3524217114446486 1105 36-37 0.3246624598097867 1105 38-39 0.3180736978858363 1105 40-41 0.33261890444575215 1105 42-43 0.3308227772487271 1105 44-45 0.2929705320673932 1105 46-47 0.24394514598536432 1105 48-49 0.3176822002252564 1105 50-51 0.3361403829409255 1105 52-53 0.304953328268482 1105 54-55 0.2721078891135349 1105 56-57 0.313678053104951 1105 58-59 0.2884032400357981 1105 60-61 0.18083649887240938 1105 62-63 0.2218023765497037 1105 64-65 0.2602054575380208 1105 66-67 0.2611888678862684 1105 68-69 0.24528204582449575 1105 70-71 0.2606987286610476 1105 72-73 0.25787957204050826 1105 74-75 0.36161072091572777 1105 76-77 0.40823794192460383 1105 78-79 0.22791675857448013 1105 80-81 0.2727679157131959 1105 82-83 0.214549986591269 1105 84-85 0.16637817300346924 1105 86-87 0.19521292576297355 1105 88-89 0.170579879304519 1105 90-91 0.19384825986841747 1105 92-93 0.17680443237286525 1105 94-95 0.08474693782073572 1105 96-97 0.1421070062591916 1105 98-99 0.11982052160045598 1105 100 0.0834417008345909 1106 1 0.22083240296171525 1106 2 0.2546201797772589 1106 3 0.2527304970740154 1106 4 0.28345267334879054 1106 5 0.26952456057257734 1106 6 0.35472738426012285 1106 7 0.26494176367451416 1106 8 0.3019508462139626 1106 9 0.4005863069892115 1106 10-11 0.366084521573228 1106 12-13 0.38009360400950243 1106 14-15 0.4677220283868735 1106 16-17 0.47868712613534825 1106 18-19 0.46809541813239264 1106 20-21 0.43796979831913063 1106 22-23 0.47828891074882307 1106 24-25 0.4932889871958679 1106 26-27 0.4514492379194053 1106 28-29 0.4936901801836058 1106 30-31 0.42041500352895866 1106 32-33 0.4444694287345996 1106 34-35 0.5601052955190937 1106 36-37 0.4897404422668714 1106 38-39 0.45371994706201946 1106 40-41 0.48677770424768596 1106 42-43 0.5024650150280081 1106 44-45 0.44732568343636814 1106 46-47 0.4463755911231715 1106 48-49 0.47009221327822814 1106 50-51 0.4928516891041994 1106 52-53 0.48622387877234985 1106 54-55 0.5520929913584496 1106 56-57 0.43404086599365144 1106 58-59 0.4981547725701816 1106 60-61 0.5657094077202203 1106 62-63 0.5102276261002245 1106 64-65 0.5324309186809586 1106 66-67 0.5284023168580347 1106 68-69 0.528676720219508 1106 70-71 0.5445574328571965 1106 72-73 0.5787186727010436 1106 74-75 0.6032191830249616 1106 76-77 0.6082234884009452 1106 78-79 0.4551821339743469 1106 80-81 0.46007091124117494 1106 82-83 0.437150548038332 1106 84-85 0.389682713227792 1106 86-87 0.39181961862777115 1106 88-89 0.407875084780116 1106 90-91 0.4497284564158548 1106 92-93 0.43011823232585655 1106 94-95 0.4470615768206194 1106 96-97 0.3409132015335956 1106 98-99 0.34343976201849813 1106 100 0.3586554311955936 1107 1 0.08935953560938614 1107 2 0.1442495119405578 1107 3 0.1271341390534566 1107 4 0.18476096745374804 1107 5 0.09042051676570395 1107 6 0.21025573404086373 1107 7 0.1888253973248979 1107 8 0.2747039218918772 1107 9 0.2786801427431769 1107 10-11 0.1977804576873723 1107 12-13 0.2561500123018732 1107 14-15 0.25092831880821365 1107 16-17 0.31456742196562004 1107 18-19 0.24189374711528444 1107 20-21 0.19545941178641613 1107 22-23 0.2426145483802955 1107 24-25 0.2750700110749733 1107 26-27 0.2903845129642093 1107 28-29 0.3259288064345114 1107 30-31 0.24903417721706944 1107 32-33 0.28637189944036123 1107 34-35 0.28374113351893016 1107 36-37 0.298656995524496 1107 38-39 0.31737120373332317 1107 40-41 0.3649404530348903 1107 42-43 0.3387002563599637 1107 44-45 0.335883919907225 1107 46-47 0.24654985681513608 1107 48-49 0.21186379879856077 1107 50-51 0.27694662920191604 1107 52-53 0.31243283151734147 1107 54-55 0.3220839991991724 1107 56-57 0.3069674017255579 1107 58-59 0.3772651995087344 1107 60-61 0.32324993131054214 1107 62-63 0.34026726348876934 1107 64-65 0.30803130489141495 1107 66-67 0.3127857992963641 1107 68-69 0.35323110945915914 1107 70-71 0.3484475487947023 1107 72-73 0.36101713306489813 1107 74-75 0.43046310169766855 1107 76-77 0.4379939807325073 1107 78-79 0.26964042299759683 1107 80-81 0.2769120914884198 1107 82-83 0.3251554041761757 1107 84-85 0.3368610901523361 1107 86-87 0.31460216206405534 1107 88-89 0.3104659235607983 1107 90-91 0.39240963820001085 1107 92-93 0.2976914896069651 1107 94-95 0.2532132734942003 1107 96-97 0.1997737939377906 1107 98-99 0.2428233560067028 1107 100 0.25468301623998 1108 1 -0.07202303718224101 1108 2 0.01672429896125749 1108 3 0.01752278087139203 1108 4 -0.008006393919735899 1108 5 0.032639375756630784 1108 6 0.12555736563392372 1108 7 0.07179059433006074 1108 8 0.042915460379155945 1108 9 0.14542412479199385 1108 10-11 0.10603169057812067 1108 12-13 0.1211360124257439 1108 14-15 0.1340079618043717 1108 16-17 0.20923758863174413 1108 18-19 0.2026972028719385 1108 20-21 0.2120837581982684 1108 22-23 0.1630100334734763 1108 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-0.5202102198504974 2215 50-51 -0.5126601594872966 2215 52-53 -0.5188735506731419 2215 54-55 -0.5696248561138262 2215 56-57 -0.5028177388279005 2215 58-59 -0.4323651885684754 2215 60-61 -0.34707186924400446 2215 62-63 -0.42600238504371646 2215 64-65 -0.4063759902074082 2215 66-67 -0.36962609316159956 2215 68-69 -0.36991058459184245 2215 70-71 -0.3607134769181286 2215 72-73 -0.4077298677854415 2215 74-75 -0.4286443221913885 2215 76-77 -0.42455329461750324 2215 78-79 -0.42311406898109283 2215 80-81 -0.47397244799533667 2215 82-83 -0.4333909143630521 2215 84-85 -0.37914565419686497 2215 86-87 -0.3176877224331349 2215 88-89 -0.3220781867291471 2215 90-91 -0.37754460699150627 2215 92-93 -0.3604602903382208 2215 94-95 -0.35194198218285067 2215 96-97 -0.31747913811843276 2215 98-99 -0.4522565070643374 2215 100 -0.4289448718773343 2216 1 -1.0710203341350706 2216 2 -1.1021235074864002 2216 3 -1.082724591547418 2216 4 -1.2297321570153628 2216 5 -1.1671118634064896 2216 6 -2.782987302349298 2216 7 -2.5959781226764846 2216 8 -3.4099488382911005 2216 9 -2.8378477775420023 2216 10-11 -2.055929382859368 2216 12-13 -1.7777606320173902 2216 14-15 -1.9624437642254833 2216 16-17 -2.3788354633748128 2216 18-19 -2.0195310817625014 2216 20-21 -1.898654009721568 2216 22-23 -1.99848751893267 2216 24-25 -2.2128283695204445 2216 26-27 -2.2143259799963886 2216 28-29 -2.6950248998297113 2216 30-31 -2.3196328428287813 2216 32-33 -2.5273049885259624 2216 34-35 -3.4039770371276177 2216 36-37 -2.510832796915885 2216 38-39 -2.374662424039748 2216 40-41 -2.918714786119253 2216 42-43 -2.787686970991963 2216 44-45 -2.4619448633473766 2216 46-47 -2.298318178112879 2216 48-49 -2.4894675239561153 2216 50-51 -2.842673051074435 2216 52-53 -3.1929731255467004 2216 54-55 -2.5351414212893175 2216 56-57 -2.5143278018041144 2216 58-59 -3.511721912449282 2216 60-61 -4.3001065183044105 2216 62-63 -3.3854442954859607 2216 64-65 -3.337665380144365 2216 66-67 -2.9865719393634436 2216 68-69 -3.4580075853034486 2216 70-71 -3.1806793018496506 2216 72-73 -3.64709862217844 2216 74-75 -4.109765625410269 2216 76-77 -4.694548351871177 2216 78-79 -3.635507031096836 2216 80-81 -3.406802551634705 2216 82-83 -3.6352254729843736 2216 84-85 -3.6819717589776424 2216 86-87 -3.297917607777187 2216 88-89 -3.1747293749626166 2216 90-91 -3.3712235866737856 2216 92-93 -3.407084961123978 2216 94-95 -3.217315876846026 2216 96-97 -3.4543419457465596 2216 98-99 -3.2436037941256615 2216 100 -2.8665125202575013 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 100676.0 3 14210.0 4 9020.0 5 7468.0 6 7160.0 7 8012.0 8 9403.0 9 10965.0 10 12203.0 11 13572.0 12 15246.0 13 16983.0 14 18957.0 15 19463.0 16 19173.0 17 19916.0 18 19181.0 19 17965.0 20 17966.0 21 19079.0 22 21388.0 23 25277.0 24 30487.0 25 38379.0 26 48101.0 27 57974.0 28 73075.0 29 93705.0 30 115350.0 31 135989.0 32 165672.0 33 213705.0 34 283397.0 35 396026.0 36 625606.0 37 1091822.0 38 1490211.0 39 582319.0 40 1596.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.23810431430165 21.03656577066893 14.817334810627997 22.90799510440142 2 15.158093189225838 20.3611293096598 41.161439524337496 23.319337976776872 3 18.181987156094717 25.47497894022434 30.718897183958422 25.62413671972252 4 12.575631874995205 17.17414196052417 39.30570701873008 30.944519145750537 5 12.853291222780626 37.97584555068018 35.97009173029009 13.200771496249104 6 30.404903493549867 38.295404006504704 17.93177905288892 13.367913447056512 7 26.228328492384907 32.92138803374846 23.13427096476268 17.716012509103958 8 24.016053231109233 37.10627464087418 20.91212160484564 17.965550523170947 9 24.2418878633507 17.46741861431954 22.330795578214936 35.95989794411482 10-11 23.365199004126737 27.4706694860339 29.369317212919064 19.794814296920297 12-13 23.723360571172684 25.092067966929882 29.355536168437823 21.82903529345961 14-15 21.230518322275326 27.42713959961993 26.545523127681943 24.7968189504228 16-17 20.6611861257812 30.748593366585126 27.304849196739912 21.28537131089376 18-19 20.557793212113356 29.720741030000326 29.317937270964674 20.403528486921648 20-21 21.683253730320146 28.931601319098295 28.8341985389924 20.55094641158916 22-23 21.347855913981796 28.811542215053354 28.830700685015486 21.009901185949364 24-25 21.18705303098059 28.920139815792854 28.797374271892217 21.095432881334343 26-27 20.98595922768846 28.822913634611158 28.879781993823432 21.31134514387695 28-29 20.984324533834485 28.860927834401107 29.03097970853561 21.123767923228804 30-31 21.274634392764803 29.189186423987163 28.628582268821766 20.90759691442627 32-33 20.976115577224505 28.942409873255748 28.928231118980808 21.15324343053894 34-35 21.169166407049715 28.609336422476673 28.99927341356989 21.22222375690372 36-37 21.337255202871493 28.868907247785426 28.572098455977013 21.221739093366075 38-39 21.16982616533476 28.848043955039593 28.803257818433437 21.1788720611922 40-41 21.06684875735722 28.58766433903654 29.07052069604843 21.27496620755781 42-43 20.844989678528712 28.55097490414315 29.097358092132392 21.506677325195746 44-45 20.91454665280938 28.632025161372283 29.021782593534535 21.4316455922838 46-47 20.866643408441714 28.762017886325253 28.896920706504392 21.474417998728644 48-49 20.763717432615287 28.641479741850688 28.917679759567182 21.67712306596684 50-51 20.747381545454296 28.55501755640011 28.903391713706544 21.794209184439055 52-53 20.677593101398536 28.539764648026694 28.960141993519308 21.82250025705547 54-55 20.767280653179682 28.751586334313377 28.798955758271 21.682177254235942 56-57 20.75369389964645 28.516418454309733 28.771131641514636 21.95875600452918 58-59 20.703352531057618 28.13127069200475 28.941242891226388 22.224133885711243 60-61 20.88024252588961 28.376758492592263 28.58443637608701 22.158562605431122 62-63 20.787408368542508 28.349023679134795 28.576942289350487 22.286625662972206 64-65 20.89504954276643 28.06982664230543 28.696005128238795 22.33911868668935 66-67 20.786957453249848 28.20535905121367 28.516098868256734 22.49158462727975 68-69 21.13403305544574 28.321455552038255 28.380035233065026 22.164476159450977 70-71 21.100301308643434 28.161901324808262 28.460439098456163 22.277358268092136 72-73 21.01681736879707 28.13566431420158 28.586304322249635 22.261213994751714 74-75 21.16317537745499 28.109025823655582 28.508983946259157 22.21881485263027 76-77 21.30271502976577 28.132947170152022 28.346828327526417 22.217509472555783 78-79 21.42451810653543 28.13927721180941 28.490046682869885 21.94615799878527 80-81 21.418462463427165 27.977786292043522 28.545772505071472 22.05797873945784 82-83 21.387913878362962 27.95001629651316 28.620402757047763 22.041667068076116 84-85 21.52738228319148 27.776423149613926 28.51140934150066 22.184785225693933 86-87 21.792049044903063 27.833603155439445 28.404002171308463 21.970345628349026 88-89 21.823464335674828 27.603310900537934 28.47557423932089 22.097650524466342 90-91 22.01834361792047 27.45182494086128 28.43575377080214 22.094077670416112 92-93 22.257790047052744 27.54894329543139 28.18167014652936 22.011596510986507 94-95 22.25871029616469 27.23858497017414 28.251011083316403 22.251693650344766 96-97 22.37504340176423 27.15353289934694 28.213430828283787 22.257992870605044 98-99 22.511713149664963 27.141550006758486 28.114405772106522 22.23233107147003 100 22.648791986359615 26.989462724937045 28.15451351927669 22.207231769426645 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1361.0 1 1127.5 2 1115.5 3 1438.0 4 1423.0 5 1172.5 6 991.0 7 924.0 8 886.5 9 844.0 10 810.5 11 815.0 12 852.5 13 957.0 14 1095.0 15 1284.0 16 1541.5 17 1975.0 18 2727.0 19 3762.5 20 5397.0 21 7725.0 22 11041.0 23 16186.5 24 23292.5 25 32408.5 26 44615.0 27 58715.0 28 74350.5 29 92666.0 30 111264.5 31 127428.5 32 141442.5 33 154470.5 34 167724.5 35 179927.0 36 189897.5 37 199955.5 38 209100.0 39 214073.5 40 217491.0 41 220179.5 42 219086.0 43 217008.0 44 213935.0 45 209578.5 46 205386.0 47 198596.0 48 191214.0 49 186001.5 50 179436.5 51 170592.5 52 160231.5 53 150071.0 54 141005.5 55 131936.5 56 119878.0 57 106138.0 58 93765.0 59 82309.5 60 71017.0 61 59004.0 62 48876.0 63 40410.5 64 32527.0 65 26120.0 66 20822.0 67 16058.0 68 12257.0 69 9480.0 70 7213.0 71 5494.5 72 4103.0 73 2936.5 74 2243.0 75 1633.0 76 1141.0 77 790.5 78 530.5 79 404.0 80 307.0 81 206.0 82 161.0 83 131.0 84 98.5 85 79.0 86 70.5 87 57.5 88 48.0 89 36.0 90 32.0 91 32.0 92 27.0 93 22.0 94 24.0 95 25.5 96 30.0 97 38.0 98 40.5 99 54.0 100 104.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01738627374142554 2 0.0404145637656078 3 0.04153955794887651 4 0.04435204340704829 5 0.047164528865220066 6 0.044880449765856326 7 0.04240887163594779 8 0.04188046527713976 9 0.04191455600996608 10-11 0.04256227993366625 12-13 0.04348272971997701 14-15 0.04208500967409771 16-17 0.03837764247923491 18-19 0.04136910428474489 20-21 0.040704334994631566 22-23 0.04176114771224763 24-25 0.038633322975432344 26-27 0.038795253956357384 28-29 0.0393747964144049 30-31 0.03936627373119832 32-33 0.039016843719728495 34-35 0.03899979835331533 36-37 0.03659640168905945 38-39 0.036800946086017396 40-41 0.0378407134372203 42-43 0.037891849536459785 44-45 0.03668162852112526 46-47 0.03588049629970663 48-49 0.035906064349326375 50-51 0.03697139975014902 52-53 0.0375679875746097 54-55 0.03672424193715817 56-57 0.03513902286073407 58-59 0.03601685923101193 60-61 0.035556634337856545 62-63 0.03585492825008689 64-65 0.03265039936441238 66-67 0.03380948428050742 68-69 0.03469584333399185 70-71 0.03600833654780535 72-73 0.03284642107816374 74-75 0.033374827436971774 76-77 0.03394584721181271 78-79 0.03441459478817467 80-81 0.03460209381871946 82-83 0.03316176035680725 84-85 0.0345850484523063 86-87 0.03461061650192604 88-89 0.03526686310883279 90-91 0.03572708800198817 92-93 0.03412482355915091 94-95 0.0353094765248657 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5866697.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.3310400377492 #Duplication Level Percentage of deduplicated Percentage of total 1 77.58871115723657 50.68951195090781 2 13.641588173369595 17.824382862657902 3 4.28068045168396 8.389839179333253 4 1.7442350859885283 4.55810768951854 5 0.8639114361604314 2.8220116312433285 6 0.49447022557079207 1.9382552462544178 7 0.30342579662965485 1.387618600766855 8 0.21146989083275827 1.105243832381871 9 0.147008330100894 0.8643786389733742 >10 0.6808438329003388 7.722903619124382 >50 0.032650786137314106 1.4414513179899882 >100 0.010743728951026621 1.1160270015221045 >500 1.827753927637412E-4 0.06954709460452936 >1k 7.832904538447115E-5 0.07072133472153516 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.7045366413162297E-5 0.0 4 0.0 1.7045366413162297E-5 0.0 6.818146565264919E-5 0.0 5 0.0 1.7045366413162297E-5 0.0 6.818146565264919E-5 0.0 6 0.0 1.7045366413162297E-5 0.0 6.818146565264919E-5 0.0 7 1.7045366413162297E-5 1.7045366413162297E-5 0.0 1.0227219847897377E-4 0.0 8 1.7045366413162297E-5 1.7045366413162297E-5 0.0 1.5340829771846065E-4 0.0 9 1.7045366413162297E-5 1.7045366413162297E-5 0.0 1.8749903054478525E-4 0.0 10-11 2.5568049619743444E-5 1.7045366413162297E-5 0.0 3.0681659543692135E-4 0.0 12-13 3.4090732826324594E-5 1.7045366413162297E-5 0.0 4.090887939158951E-4 0.0 14-15 5.113609923948689E-5 1.7045366413162297E-5 0.0 4.687475763619631E-4 0.0 16-17 5.113609923948689E-5 4.2613416032905744E-5 0.0 6.051105076672615E-4 0.0 18-19 5.965878244606804E-5 6.818146565264919E-5 0.0 6.818146565264918E-4 0.0 20-21 6.818146565264919E-5 6.818146565264919E-5 0.0 9.971539351699944E-4 8.522683206581148E-6 22-23 8.522683206581147E-5 7.670414885923034E-5 0.0 0.0020369212863728943 1.7045366413162297E-5 24-25 8.522683206581147E-5 9.374951527239262E-5 0.0 0.004346568435356385 1.7045366413162297E-5 26-27 8.522683206581147E-5 1.0227219847897377E-4 0.0 0.008820977118811489 1.7045366413162297E-5 28-29 8.522683206581147E-5 1.0227219847897377E-4 0.0 0.02931803023063915 1.7045366413162297E-5 30-31 1.1931756489213608E-4 1.0227219847897377E-4 0.0 0.0888830631614348 1.7045366413162297E-5 32-33 1.1931756489213608E-4 1.0227219847897377E-4 0.0 0.22538235739803844 1.7045366413162297E-5 34-35 1.1931756489213608E-4 1.0227219847897377E-4 0.0 0.4355858160051559 1.7045366413162297E-5 36-37 1.1931756489213608E-4 1.278402480987172E-4 0.0 0.752050088831927 1.7045366413162297E-5 38-39 1.1931756489213608E-4 1.5340829771846065E-4 0.0 1.1490196272280637 1.7045366413162297E-5 40-41 1.7045366413162295E-4 1.5340829771846065E-4 0.0 1.5387107941657803 1.7045366413162297E-5 42-43 1.7045366413162295E-4 1.7045366413162295E-4 0.0 1.9191207590915296 1.7045366413162297E-5 44-45 1.7045366413162295E-4 1.7045366413162295E-4 0.0 2.3672178740439467 1.7045366413162297E-5 46-47 1.7045366413162295E-4 1.7045366413162295E-4 0.0 2.888396997492797 1.7045366413162297E-5 48-49 1.7045366413162295E-4 1.7045366413162295E-4 0.0 3.405519323735315 1.7045366413162297E-5 50-51 1.8749903054478525E-4 1.7045366413162295E-4 0.0 3.892624759724254 1.7045366413162297E-5 52-53 1.8749903054478525E-4 1.7045366413162295E-4 0.0 4.346363890959427 1.7045366413162297E-5 54-55 1.8749903054478525E-4 1.8749903054478525E-4 0.0 4.836136926792026 1.7045366413162297E-5 56-57 1.8749903054478525E-4 1.8749903054478525E-4 0.0 5.389787473257951 1.7045366413162297E-5 58-59 2.2158976337110985E-4 1.8749903054478525E-4 0.0 5.96192371959895 1.7045366413162297E-5 60-61 2.2158976337110985E-4 1.8749903054478525E-4 0.0 6.515514607282428 1.7045366413162297E-5 62-63 2.2158976337110985E-4 1.8749903054478525E-4 0.0 7.041739159189575 1.7045366413162297E-5 64-65 2.2158976337110985E-4 1.8749903054478525E-4 0.0 7.582776134509759 1.7045366413162297E-5 66-67 2.2158976337110985E-4 1.8749903054478525E-4 0.0 8.16612823195062 1.7045366413162297E-5 68-69 2.2158976337110985E-4 2.130670801645287E-4 0.0 8.783741515881935 1.7045366413162297E-5 70-71 2.2158976337110985E-4 2.2158976337110985E-4 0.0 9.396726982832078 1.7045366413162297E-5 72-73 2.30112446577691E-4 2.2158976337110985E-4 0.0 9.992573333853786 1.7045366413162297E-5 74-75 2.3863512978427215E-4 2.2158976337110985E-4 0.0 10.573956691473924 1.7045366413162297E-5 76-77 2.3863512978427215E-4 2.3863512978427215E-4 0.0 11.17489108436996 1.7045366413162297E-5 78-79 2.556804961974344E-4 2.556804961974344E-4 0.0 11.820833767279954 1.7045366413162297E-5 80-81 2.556804961974344E-4 2.556804961974344E-4 0.0 12.478392867400515 1.7045366413162297E-5 82-83 2.556804961974344E-4 2.556804961974344E-4 0.0 13.11826739986742 1.7045366413162297E-5 84-85 2.556804961974344E-4 2.556804961974344E-4 0.0 13.737397721409508 1.7045366413162297E-5 86-87 2.556804961974344E-4 2.642031794040156E-4 0.0 14.366627422551396 1.7045366413162297E-5 88 2.556804961974344E-4 2.7272586261059675E-4 0.0 14.85616182325421 1.7045366413162297E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5850 0.0 46.61401 1 GTATCAA 9800 0.0 35.50177 1 TCAACGC 10255 0.0 32.73124 4 ATCAACG 10420 0.0 32.167824 3 CAACGCA 10865 0.0 30.807316 5 AACGCAG 11590 0.0 28.793915 6 TATCAAC 12250 0.0 27.707735 2 ACGCAGA 13250 0.0 24.76084 7 GTACATG 14750 0.0 24.033869 1 CGCAGAG 13365 0.0 23.949507 8 TACATGG 14720 0.0 22.994534 2 ACATGGG 14955 0.0 21.658716 3 GCAGAGT 15610 0.0 19.812765 9 CATGGGG 10690 0.0 18.470194 4 GTGGTAT 3760 0.0 18.256157 1 CAGAGTA 14590 0.0 18.121302 10-11 AGAGTAC 13020 0.0 17.165707 10-11 GAGTACT 6935 0.0 16.300117 12-13 GAGTACA 10885 0.0 16.240664 1 TGGTATC 3840 0.0 16.160048 2 >>END_MODULE