FastQCFastQC Report
Wed 25 May 2016
SRR2049367_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049367_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5866697
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT155400.26488499406054206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137360.2341351530511973No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132300.22551019764613717No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76080.12968114767133873No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT74640.12722661490784337RNA PCR Primer, Index 46 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98100.060.046951
GTATCAA179150.039.5462531
ATCAACG219650.032.0087433
TCAACGC220350.031.8217434
CAACGCA226750.030.9443045
TATCAAC231850.030.4257872
GTGGTAT43200.030.2531011
AACGCAG235050.029.8826836
TGGTATC41300.029.5863572
ACGCAGA265900.026.2212627
CGCAGAG268650.025.7076848
GCAGAGT293550.023.1099789
GTACATG141550.022.052971
GAGTACT210550.021.96390212-13
CAGAGTA286750.021.07400110-11
AGAGTAC268950.020.62331810-11
TACATGG145300.020.1830672
GTACTTT229350.019.97867414-15
ACATGGG145000.019.2848913
AGTACTT222350.019.23339512-13
Produced by FastQC (version 0.11.5)