##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049367_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5866697
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.740513955297164	31.0	31.0	34.0	30.0	34.0
2	31.86149463659023	31.0	31.0	34.0	30.0	34.0
3	31.902034483798975	33.0	31.0	34.0	30.0	34.0
4	35.66245981341801	37.0	35.0	37.0	33.0	37.0
5	35.72011576531053	37.0	35.0	37.0	33.0	37.0
6	35.805308165736186	37.0	35.0	37.0	35.0	37.0
7	35.75553296855113	37.0	35.0	37.0	33.0	37.0
8	35.76714580623475	37.0	35.0	37.0	33.0	37.0
9	37.49559965343361	39.0	37.0	39.0	35.0	39.0
10-11	37.45527142444888	39.0	37.0	39.0	34.0	39.0
12-13	37.39873739857367	39.0	37.0	39.0	34.0	39.0
14-15	38.67163814664367	40.0	38.0	41.0	34.0	41.0
16-17	38.69631250770237	40.0	38.0	41.0	34.0	41.0
18-19	38.77197603694208	40.0	38.0	41.0	34.5	41.0
20-21	38.80930811664553	40.0	38.0	41.0	34.5	41.0
22-23	38.778383219723125	40.0	38.0	41.0	34.5	41.0
24-25	38.75709082981446	40.0	38.0	41.0	34.0	41.0
26-27	38.63803414425527	40.0	38.0	41.0	34.0	41.0
28-29	38.55649149768601	40.0	38.0	41.0	34.0	41.0
30-31	38.45083289626173	40.0	38.0	41.0	34.0	41.0
32-33	38.308296900283075	40.0	38.0	41.0	34.0	41.0
34-35	38.194381779048754	40.0	38.0	41.0	34.0	41.0
36-37	38.06140993816453	40.0	38.0	41.0	33.0	41.0
38-39	37.956442782028795	40.0	37.5	41.0	33.0	41.0
40-41	37.85779741138839	40.0	37.0	41.0	33.0	41.0
42-43	37.71948261517511	40.0	37.0	41.0	33.0	41.0
44-45	37.545125732588545	40.0	37.0	41.0	33.0	41.0
46-47	37.32366687763148	40.0	37.0	41.0	32.0	41.0
48-49	37.11810052573024	40.0	37.0	41.0	31.5	41.0
50-51	36.96253752324348	40.0	37.0	41.0	31.0	41.0
52-53	36.835850053275294	40.0	36.0	41.0	31.0	41.0
54-55	36.70053396314826	40.0	36.0	41.0	31.0	41.0
56-57	36.56254967658974	39.0	36.0	41.0	31.0	41.0
58-59	36.38107993305262	39.0	35.0	41.0	30.5	41.0
60-61	36.20576799858591	39.0	35.0	41.0	30.0	41.0
62-63	36.00880904536232	39.0	35.0	41.0	30.0	41.0
64-65	35.76870579816888	38.5	35.0	40.5	29.5	41.0
66-67	35.467938773725656	38.0	35.0	40.0	29.0	41.0
68-69	35.139644249566665	37.0	35.0	40.0	29.0	41.0
70-71	34.77878898467059	37.0	34.0	39.5	28.0	41.0
72-73	34.47421888330009	36.0	34.0	39.0	28.0	41.0
74-75	34.153229065690624	36.0	34.0	39.0	28.0	41.0
76-77	33.8064640972595	35.5	34.0	38.5	28.0	40.0
78-79	33.45060346562981	35.0	34.0	37.0	27.0	39.5
80-81	33.10936307090685	35.0	34.0	37.0	27.0	39.0
82-83	32.789305208706025	35.0	33.0	36.5	26.0	39.0
84-85	32.50136627134485	35.0	33.0	36.0	26.0	37.5
86-87	32.24581156995154	35.0	33.0	36.0	26.0	37.0
88-89	31.99218163133361	35.0	33.0	35.0	26.0	37.0
90-91	31.775577381957856	35.0	33.0	35.0	25.0	36.0
92-93	31.575652790658864	35.0	33.0	35.0	25.0	36.0
94-95	31.401662894811167	35.0	33.0	35.0	24.5	36.0
96-97	31.18477671507494	35.0	33.0	35.0	24.0	36.0
98-99	31.01224735144835	35.0	33.0	35.0	23.5	35.0
100	30.88220663177253	35.0	33.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1422715575333804
1101	2	0.18888774390950402
1101	3	0.3795432679700568
1101	4	0.20287273253407534
1101	5	0.19152731462556005
1101	6	0.19742665721101105
1101	7	0.03231825982069836
1101	8	0.13190632027744442
1101	9	0.255984017809773
1101	10-11	0.26925436572998507
1101	12-13	0.21435852844938807
1101	14-15	0.33114223466568404
1101	16-17	0.26901069915302145
1101	18-19	0.33582372864949406
1101	20-21	0.29374395656794405
1101	22-23	0.2673098546288628
1101	24-25	0.2608613553508903
1101	26-27	0.3189050231015713
1101	28-29	0.3231113269229624
1101	30-31	0.3455379319701777
1101	32-33	0.3919344176587245
1101	34-35	0.3878537905779993
1101	36-37	0.3572206659319903
1101	38-39	0.29776419298652
1101	40-41	0.31438478664617264
1101	42-43	0.3944594271793562
1101	44-45	0.3735826328616483
1101	46-47	0.38648614468571907
1101	48-49	0.35991744271519366
1101	50-51	0.3457329781908527
1101	52-53	0.3884783020069378
1101	54-55	0.38794211214089813
1101	56-57	0.3394204209891427
1101	58-59	0.3524830185466499
1101	60-61	0.40552026162745136
1101	62-63	0.40566073421352655
1101	64-65	0.3245342452732842
1101	66-67	0.3404391744837696
1101	68-69	0.38186342680373286
1101	70-71	0.40401830516502457
1101	72-73	0.33821128172189674
1101	74-75	0.41721373131832706
1101	76-77	0.363935205594764
1101	78-79	0.37883687957368295
1101	80-81	0.4353131902266085
1101	82-83	0.4255163205639221
1101	84-85	0.4212258299676819
1101	86-87	0.369053363022509
1101	88-89	0.44487687051361036
1101	90-91	0.44247621809103777
1101	92-93	0.48594283981965347
1101	94-95	0.5335212946200691
1101	96-97	0.5844376291149942
1101	98-99	0.6631935080480673
1101	100	0.684024271907056
1102	1	0.19980444600068736
1102	2	0.19004972798019537
1102	3	0.35758914197111125
1102	4	0.21613818523402273
1102	5	0.17500123218307806
1102	6	0.157934128430945
1102	7	0.18000618255010892
1102	8	0.2000023536786415
1102	9	0.15786170964957336
1102	10-11	0.24267019602766027
1102	12-13	0.14428324042594198
1102	14-15	0.2656872746061012
1102	16-17	0.20918338022993765
1102	18-19	0.26699448996060227
1102	20-21	0.25123327809741625
1102	22-23	0.24462174240807855
1102	24-25	0.25173012207810785
1102	26-27	0.3397614313162478
1102	28-29	0.3216154475679147
1102	30-31	0.3119810998782313
1102	32-33	0.3055292454124441
1102	34-35	0.30544231555004586
1102	36-37	0.32077770795740435
1102	38-39	0.2696733716316828
1102	40-41	0.30086376176290486
1102	42-43	0.34470386186364976
1102	44-45	0.3573418670059638
1102	46-47	0.3150623901114571
1102	48-49	0.2741617779911678
1102	50-51	0.33661961452880007
1102	52-53	0.41880020627718295
1102	54-55	0.25674198311219953
1102	56-57	0.3448369572512888
1102	58-59	0.3632193241429036
1102	60-61	0.33836176263616835
1102	62-63	0.3840449134799968
1102	64-65	0.32825213492646554
1102	66-67	0.27001182297276927
1102	68-69	0.34641331175146206
1102	70-71	0.375587180272106
1102	72-73	0.38349958216619484
1102	74-75	0.349680544717927
1102	76-77	0.3325044896816536
1102	78-79	0.3764541346535566
1102	80-81	0.3961848294133574
1102	82-83	0.4398041998090889
1102	84-85	0.39362573384846655
1102	86-87	0.36369941633522984
1102	88-89	0.32355052828649633
1102	90-91	0.35236746566135935
1102	92-93	0.40866520289329955
1102	94-95	0.3583575821124292
1102	96-97	0.44964334255782745
1102	98-99	0.5085177406504968
1102	100	0.5265795417172754
1103	1	0.06339315202842855
1103	2	0.11871561149446919
1103	3	0.2830404030891671
1103	4	0.1715565912779411
1103	5	0.13681138142801785
1103	6	0.10727749571811529
1103	7	0.13538818225938343
1103	8	0.11874122422709377
1103	9	0.17923577672082303
1103	10-11	0.14061368936574326
1103	12-13	0.17998563165183867
1103	14-15	0.2580739477321572
1103	16-17	0.2053796192233719
1103	18-19	0.2280318479419421
1103	20-21	0.2692813789703905
1103	22-23	0.23910900847108962
1103	24-25	0.24020826193608258
1103	26-27	0.22770428852919622
1103	28-29	0.26589233233175236
1103	30-31	0.24232379958449712
1103	32-33	0.22963099686819533
1103	34-35	0.23157296443535103
1103	36-37	0.21616152053566395
1103	38-39	0.23650939127936255
1103	40-41	0.24000091323139117
1103	42-43	0.2823892504988663
1103	44-45	0.32486281642008663
1103	46-47	0.3373310190927086
1103	48-49	0.2750531198463051
1103	50-51	0.2633666958228673
1103	52-53	0.2865087206016881
1103	54-55	0.2656842288214065
1103	56-57	0.27139241242291234
1103	58-59	0.3181376578560702
1103	60-61	0.27559034158257845
1103	62-63	0.23765986098345593
1103	64-65	0.3208226797571214
1103	66-67	0.23380164880854437
1103	68-69	0.2523069891321086
1103	70-71	0.31566425918552454
1103	72-73	0.28300291827446244
1103	74-75	0.2810840444049916
1103	76-77	0.25387714606219447
1103	78-79	0.23606734341501578
1103	80-81	0.2748630192325692
1103	82-83	0.2065285514587032
1103	84-85	0.19472741319250986
1103	86-87	0.20979573286408026
1103	88-89	0.2641672621099289
1103	90-91	0.26650923692285033
1103	92-93	0.3406911589013042
1103	94-95	0.26278698882761375
1103	96-97	0.3120659944302915
1103	98-99	0.35227217917851306
1103	100	0.39552278279581543
1104	1	0.15536328375830166
1104	2	0.18713455167529958
1104	3	0.27484405834969294
1104	4	0.16657651859604528
1104	5	0.1628634836882057
1104	6	0.1801970641264461
1104	7	0.18943506593292625
1104	8	0.2075718294545439
1104	9	0.2256870030872946
1104	10-11	0.21827015009572648
1104	12-13	0.200116826831227
1104	14-15	0.2646891687672692
1104	16-17	0.2568140778109793
1104	18-19	0.2951288577401243
1104	20-21	0.28894834409199177
1104	22-23	0.27546130584497774
1104	24-25	0.2914286912528823
1104	26-27	0.2857706407974092
1104	28-29	0.29796103057520185
1104	30-31	0.28303504589148787
1104	32-33	0.3191539022776979
1104	34-35	0.30243395068100654
1104	36-37	0.27602367157497554
1104	38-39	0.28217103837563684
1104	40-41	0.30090323319424783
1104	42-43	0.3664264597327218
1104	44-45	0.3081080923534145
1104	46-47	0.3839321534436735
1104	48-49	0.3909878427935993
1104	50-51	0.30015002378846134
1104	52-53	0.2938921888091599
1104	54-55	0.266880381766434
1104	56-57	0.28741351231198564
1104	58-59	0.3738980464557855
1104	60-61	0.3311973224004987
1104	62-63	0.34050443234647787
1104	64-65	0.268285021174286
1104	66-67	0.3547980230463068
1104	68-69	0.3407324858776377
1104	70-71	0.38574726271157544
1104	72-73	0.3507210448774174
1104	74-75	0.3961997800630712
1104	76-77	0.3478676746663041
1104	78-79	0.35864736204449343
1104	80-81	0.2962718904132231
1104	82-83	0.35612918569974994
1104	84-85	0.3724902983143039
1104	86-87	0.41660608843099567
1104	88-89	0.3819438922689855
1104	90-91	0.36143691958345414
1104	92-93	0.4012699837110354
1104	94-95	0.42438319221416876
1104	96-97	0.4525134157393609
1104	98-99	0.46408891145943443
1104	100	0.4534666491024488
1105	1	0.13995702655694942
1105	2	0.12565535761573443
1105	3	0.24824756255900837
1105	4	0.13218822954235065
1105	5	0.12704403378423734
1105	6	0.11668303661203083
1105	7	0.12165817559709069
1105	8	0.10723513835637988
1105	9	0.18083794078999915
1105	10-11	0.17496582000867988
1105	12-13	0.12802260767044515
1105	14-15	0.206032450994158
1105	16-17	0.21033287126639522
1105	18-19	0.16541101854659246
1105	20-21	0.17871477984480322
1105	22-23	0.14801605368918302
1105	24-25	0.21596477087107502
1105	26-27	0.2580172958405029
1105	28-29	0.2442986955398183
1105	30-31	0.22836841686985565
1105	32-33	0.25094388195136474
1105	34-35	0.19302792107304612
1105	36-37	0.1977772329908305
1105	38-39	0.22436375961527233
1105	40-41	0.21461476127714718
1105	42-43	0.22237222463643036
1105	44-45	0.23436292631063793
1105	46-47	0.24900569944892226
1105	48-49	0.29544671164909886
1105	50-51	0.23898218333236088
1105	52-53	0.2642409640962029
1105	54-55	0.23475850278342847
1105	56-57	0.2323924566086646
1105	58-59	0.24917445081940315
1105	60-61	0.20288368278900748
1105	62-63	0.2339158353855595
1105	64-65	0.17968749027769348
1105	66-67	0.23777851039178444
1105	68-69	0.21203324976035276
1105	70-71	0.19837388473706596
1105	72-73	0.25881738515035124
1105	74-75	0.20857378269332116
1105	76-77	0.197100169940299
1105	78-79	0.2243091978854892
1105	80-81	0.2455397441842706
1105	82-83	0.2223813394365095
1105	84-85	0.16630478159211748
1105	86-87	0.13259604250868762
1105	88-89	0.17964349562419102
1105	90-91	0.18745030173073118
1105	92-93	0.23410794019271108
1105	94-95	0.19426764780366312
1105	96-97	0.2652893453977576
1105	98-99	0.23142040403007513
1105	100	0.2767554850762046
1106	1	0.15391824707444712
1106	2	0.12614969628957695
1106	3	0.25814698446927054
1106	4	0.14842679538410408
1106	5	0.09884163734136564
1106	6	0.13386688082429998
1106	7	0.13729532635053943
1106	8	0.11406320569111017
1106	9	0.12637884310978365
1106	10-11	0.14222653417917286
1106	12-13	0.15197499604801834
1106	14-15	0.24924883240535678
1106	16-17	0.16048405717224767
1106	18-19	0.17516586041300286
1106	20-21	0.18732619948551843
1106	22-23	0.1612168383529351
1106	24-25	0.20689326446313316
1106	26-27	0.20950154085960548
1106	28-29	0.209505805956546
1106	30-31	0.2580194302039658
1106	32-33	0.14374643043148438
1106	34-35	0.1546264257414478
1106	36-37	0.17832405754271718
1106	38-39	0.2358211728006765
1106	40-41	0.1876776242474989
1106	42-43	0.23877156512560305
1106	44-45	0.1785252133932076
1106	46-47	0.15878172232056187
1106	48-49	0.18494281495863163
1106	50-51	0.17920779272313325
1106	52-53	0.13834956318746094
1106	54-55	0.1369189525146126
1106	56-57	0.14896619313513781
1106	58-59	0.18772014213310229
1106	60-61	0.1675213960648989
1106	62-63	0.17251480246429907
1106	64-65	0.12580387711697227
1106	66-67	0.15937338695689363
1106	68-69	0.17356710403504394
1106	70-71	0.1596848103719566
1106	72-73	0.25182308949143106
1106	74-75	0.23292413585090088
1106	76-77	0.19095831852712308
1106	78-79	0.19551277954290924
1106	80-81	0.19700790981831773
1106	82-83	0.24886223031637655
1106	84-85	0.17364468801419264
1106	86-87	0.16102465131926635
1106	88-89	0.18303923515043863
1106	90-91	0.16189699677700276
1106	92-93	0.2594782397250235
1106	94-95	0.19880713600810296
1106	96-97	0.16281866339115325
1106	98-99	0.1918822651625618
1106	100	0.21489199700695494
1107	1	0.12469781956575332
1107	2	0.10572429464805566
1107	3	0.22315775522932313
1107	4	0.16549628104002778
1107	5	0.12985287413200552
1107	6	0.08339725585497604
1107	7	0.05395864652098936
1107	8	0.10166722594856736
1107	9	0.15513398369980536
1107	10-11	0.1414446625210246
1107	12-13	0.10631704390539909
1107	14-15	0.21783855692675047
1107	16-17	0.1488037008571652
1107	18-19	0.16653410018078318
1107	20-21	0.12024834758904746
1107	22-23	0.09200116410831782
1107	24-25	0.12550545388969425
1107	26-27	0.22862639491519587
1107	28-29	0.2194949422382635
1107	30-31	0.15399811922026885
1107	32-33	0.18996454885778746
1107	34-35	0.1326977346180982
1107	36-37	0.21399442270742952
1107	38-39	0.1967975254668488
1107	40-41	0.19884770186142475
1107	42-43	0.27019971957011535
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2216	80-81	-1.0078753833876704
2216	82-83	-0.8530444392075651
2216	84-85	-0.9325568600857252
2216	86-87	-0.7708409729897774
2216	88-89	-1.1504747525938797
2216	90-91	-0.9796052408353155
2216	92-93	-1.0750226231302484
2216	94-95	-1.0339384883100244
2216	96-97	-1.087111815580922
2216	98-99	-1.227121702246432
2216	100	-1.1738166076191838
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	353.0
3	32.0
4	56.0
5	97.0
6	242.0
7	777.0
8	1775.0
9	3260.0
10	5056.0
11	7161.0
12	9992.0
13	13878.0
14	17007.0
15	21554.0
16	26816.0
17	35842.0
18	35038.0
19	20644.0
20	16670.0
21	16976.0
22	18765.0
23	21501.0
24	26586.0
25	32684.0
26	38577.0
27	42964.0
28	55353.0
29	72311.0
30	94080.0
31	121067.0
32	156479.0
33	204037.0
34	275470.0
35	394689.0
36	630064.0
37	1141459.0
38	1630748.0
39	673984.0
40	2653.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.10027096897626	21.524308343631155	15.178764326220662	23.196656361171925
2	15.125768383811197	20.683256694525046	40.51850640999526	23.67246851166849
3	17.371273818981958	25.714315908934786	31.692705452488855	25.221704819594397
4	12.456480989532597	17.94918735252542	38.5181829137355	31.076148744206485
5	12.244521917528722	38.08026901679088	36.2494091649867	13.425799900693693
6	29.8665330764483	39.00484378177363	17.31580819667353	13.812814945104545
7	25.52922027505426	33.50779834036086	22.785529915726006	18.177451468858884
8	24.0449524387448	37.200018068248546	20.48746770939779	18.26756178360886
9	24.01209743745075	16.959986172798764	22.154493405744322	36.87342298400616
10-11	23.26786264911926	27.484426074842457	29.068733224163445	20.178978051874846
12-13	24.00198255045242	24.903722414970183	29.428383093343154	21.665911941234246
14-15	21.299303465779122	27.066695791553315	26.988585300108348	24.645415442559216
16-17	20.23723059159183	30.532700086607505	27.800319327894385	21.42974999390628
18-19	20.07640930309302	28.690332238224798	30.795487202843713	20.437771255838467
20-21	21.017696173584703	27.843902492239238	30.699608910779702	20.438792423396354
22-23	20.737452437035696	27.919602802053696	30.66522781046984	20.677716950440768
24-25	20.741243895090804	28.066853682888613	30.626456048275923	20.56544637374466
26-27	20.54427928233516	27.95473907451113	30.809839408702754	20.691142234450954
28-29	20.437340827857923	27.788649906599694	31.086100953468936	20.68790831207345
30-31	20.796778834836708	28.31388769523976	30.50905816339245	20.380275306531086
32-33	20.7324967057651	27.887828031094543	30.79344505848572	20.586230204654633
34-35	20.724634630284566	27.633499579703695	30.817848108504712	20.824017681507023
36-37	20.777294541475225	28.043285018225767	30.400549201893057	20.77887123840595
38-39	20.695149082514074	27.80979851650438	30.649852625435425	20.845199775546117
40-41	20.587825452868188	27.60765204845901	30.82085206301577	20.983670435657036
42-43	20.624045242670718	27.52565593383917	30.749748494632563	21.100550328857544
44-45	20.564654485454735	27.77008651393419	30.481225768896746	21.184033231714334
46-47	20.76054601800367	27.868494226645236	30.023737393451473	21.347222361899625
48-49	20.686677209296775	27.80954061149811	29.853234145678353	21.650548033526757
50-51	20.550088141875182	27.82191216221514	29.794425397132756	21.833574298776924
52-53	20.462197504052344	27.878210967909478	29.91140032688052	21.748191201157656
54-55	20.55822908281561	28.118641258190856	29.552749405434337	21.77038025355919
56-57	20.570203735506055	27.876940515709496	29.435122961473066	22.117732787311382
58-59	20.34046108786026	27.772930260005175	29.705527357755845	22.18108129437872
60-61	20.563638291315304	28.18979805982524	29.098411233050065	22.148152415809395
62-63	20.23406357354635	28.32524297495642	29.105133863357207	22.33555958814002
64-65	20.239818957090776	28.123594018452184	29.152038492669313	22.48454853178772
66-67	20.28699129175485	28.246909977527107	28.899627792747584	22.566470937970458
68-69	20.64400675312708	28.355062642165656	28.64916747995501	22.351763124752253
70-71	20.6529454964834	28.17828799661955	28.590906623196283	22.577859883700764
72-73	20.512599358270688	28.198299949136203	28.641508745709643	22.647591946883466
74-75	20.71623767054475	28.16049624299557	28.46740946524434	22.65585662121534
76-77	20.82761316222877	28.154091267510374	28.25246911959876	22.765826450662097
78-79	20.823533693303816	28.191479433348626	28.30872993341259	22.676256939934962
80-81	20.669405357492902	28.084299971874437	28.38030869932073	22.86598597131193
82-83	20.686782116153466	27.982906725916983	28.4425143203898	22.88779683753975
84-85	20.785869305083672	27.83496359358178	28.271148552777547	23.108018548557006
86-87	20.873189190451484	27.985849407566672	28.189433777721185	22.951527624260656
88-89	20.68683792884117	27.74675128040943	28.32257223777974	23.243838552969656
90-91	20.637589383879792	27.573011395112974	28.36760958314512	23.421789637862116
92-93	20.708339668671474	27.64675370455347	28.275767553204904	23.36913907357015
94-95	20.576921798477013	27.36536812343039	28.534029762565293	23.523680315527308
96-97	20.69075022059446	27.329729331686366	28.445780042715167	23.53374040500401
98-99	20.650138884947047	27.401291373042046	28.39455035428425	23.55401938772666
100	20.675840603277525	27.385138290872767	28.35711193157218	23.581909174277524
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	498.0
1	394.5
2	477.0
3	683.5
4	471.0
5	347.5
6	872.5
7	2413.0
8	4197.5
9	4901.5
10	3882.0
11	2527.0
12	2397.5
13	2772.5
14	3240.5
15	3833.5
16	4485.5
17	5167.5
18	6013.0
19	7134.0
20	8734.0
21	11015.0
22	14210.5
23	18902.0
24	25642.0
25	34275.0
26	45635.0
27	59298.0
28	74659.0
29	92492.5
30	110740.0
31	126352.0
32	139523.0
33	151515.5
34	164422.5
35	176412.0
36	185971.5
37	195424.0
38	204815.5
39	211185.0
40	216003.0
41	219852.5
42	220232.5
43	219906.5
44	218213.0
45	215130.5
46	211070.5
47	203792.5
48	195350.5
49	188360.5
50	180749.0
51	171343.5
52	159974.0
53	148962.0
54	139359.0
55	129270.5
56	116542.5
57	102523.5
58	90314.0
59	79535.5
60	68158.5
61	56184.5
62	46517.5
63	38024.0
64	30175.0
65	23791.0
66	18438.5
67	14030.5
68	10503.0
69	7950.5
70	5903.0
71	4231.5
72	2963.5
73	1970.5
74	1388.0
75	943.0
76	571.0
77	343.5
78	193.0
79	102.0
80	57.0
81	28.5
82	17.0
83	14.5
84	6.5
85	2.0
86	2.0
87	1.5
88	1.5
89	1.0
90	0.5
91	0.5
92	0.5
93	0.5
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	3.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0372952617119991
2	0.0
3	0.0
4	1.3636293130529838E-4
5	0.0
6	0.0
7	0.0
8	8.863590534844394E-4
9	0.0
10-11	0.0
12-13	0.043184435807746675
14-15	1.278402480987172E-4
16-17	0.0
18-19	5.1136099239486894E-5
20-21	8.522683206581147E-5
22-23	0.0
24-25	7.670414885923034E-5
26-27	5.1136099239486894E-5
28-29	2.3863512978427215E-4
30-31	0.0
32-33	1.448856145118795E-4
34-35	4.261341603290574E-5
36-37	3.4090732826324594E-5
38-39	2.2158976337110985E-4
40-41	1.1079488168555493E-4
42-43	3.920434275027328E-4
44-45	2.0454439695794758E-4
46-47	6.818146565264919E-5
48-49	6.732919733199107E-4
50-51	3.238619618500836E-4
52-53	4.6874757636196314E-4
54-55	3.323846450566648E-4
56-57	3.749980610895705E-4
58-59	1.0227219847897379E-4
60-61	1.5340829771846068E-4
62-63	7.329507557659787E-4
64-65	3.6647537788298934E-4
66-67	0.0012443117481608476
68-69	0.001389197362672727
70-71	3.4943001146982706E-4
72-73	8.352229542449525E-4
74-75	9.374951527239263E-4
76-77	5.284063588080312E-4
78-79	0.0033664598665995534
80-81	0.0025056688627348575
82-83	0.0010568127176160623
84-85	0.002539759595561182
86-87	0.0014914695611517009
88-89	0.001985785187133407
90-91	0.0025056688627348575
92-93	0.002267033732950585
94-95	0.0034090732826324594
96-97	0.002769872042138873
98-99	0.0025653276451809257
100	0.0013124932138134967
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5866697.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.77837005199708
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.28627303350454	47.89127878385034
2	14.531424818620378	18.245183292922494
3	4.419981758627064	8.324377513984999
4	1.8397392663518835	4.619833298489127
5	0.9017742295781466	2.830595814390573
6	0.5185333257833056	1.953160620619027
7	0.3370628174771501	1.4812178000444494
8	0.22332013318849542	1.1215739169094912
9	0.15006840899235385	0.8478945101552791
>10	0.7399616772816084	8.078094376650423
>50	0.03682890833994816	1.565084812908322
>100	0.01402747028497549	1.4846788272257034
>500	5.699313525058755E-4	0.24058917557372364
>1k	2.9852667483541126E-4	0.3354642231321825
>5k	5.42775771630619E-5	0.2567860216670201
>10k+	8.141636574459285E-5	0.7241870114768018
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT	15540	0.26488499406054206	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13736	0.2341351530511973	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13230	0.22551019764613717	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7608	0.12968114767133873	No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	7464	0.12722661490784337	RNA PCR Primer, Index 46 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.7045366413162297E-5	0.0	0.0	0.0
3	0.0	1.7045366413162297E-5	0.0	0.0	0.0
4	0.0	1.7045366413162297E-5	0.0	5.113609923948689E-5	0.0
5	0.0	1.7045366413162297E-5	0.0	6.818146565264919E-5	0.0
6	0.0	1.7045366413162297E-5	0.0	6.818146565264919E-5	0.0
7	0.0	1.7045366413162297E-5	0.0	6.818146565264919E-5	0.0
8	0.0	1.7045366413162297E-5	0.0	8.522683206581147E-5	0.0
9	0.0	3.4090732826324594E-5	0.0	1.3636293130529838E-4	0.0
10-11	0.0	3.4090732826324594E-5	0.0	2.556804961974344E-4	0.0
12-13	0.0	3.4090732826324594E-5	0.0	3.8352074429615166E-4	0.0
14-15	0.0	6.818146565264917E-5	0.0	4.60224893155382E-4	0.0
16-17	0.0	8.522683206581147E-5	0.0	6.051105076672615E-4	0.0
18-19	0.0	1.0227219847897377E-4	0.0	6.732919733199107E-4	0.0
20-21	0.0	1.1931756489213608E-4	0.0	9.460178359305074E-4	0.0
22-23	0.0	1.2784024809871724E-4	0.0	0.0019943078703399887	0.0
24-25	0.0	1.3636293130529838E-4	0.0	0.004329523068943223	0.0
26-27	0.0	1.3636293130529838E-4	0.0	0.008948817366910205	0.0
28-29	0.0	1.3636293130529838E-4	0.0	0.029428825112324704	0.0
30-31	0.0	1.3636293130529838E-4	0.0	0.08907056219197958	0.0
32-33	0.0	1.3636293130529838E-4	0.0	0.2258255369247807	0.0
34-35	0.0	1.3636293130529838E-4	0.0	0.43669376482201144	0.0
36-37	0.0	1.5340829771846068E-4	0.0	0.7543853040305304	0.0
38-39	8.522683206581148E-6	1.7045366413162295E-4	0.0	1.152999720285537	0.0
40-41	3.4090732826324594E-5	2.0454439695794755E-4	0.0	1.5446425816775606	0.0
42-43	3.4090732826324594E-5	2.2158976337110985E-4	0.0	1.9266718564125607	0.0
44-45	3.4090732826324594E-5	2.2158976337110985E-4	0.0	2.3764223719070543	0.0
46-47	3.4090732826324594E-5	2.2158976337110985E-4	0.0	2.89973216615755	0.0
48-49	6.818146565264919E-5	2.2158976337110985E-4	0.0	3.4190107312513325	0.0
50-51	6.818146565264919E-5	2.3863512978427215E-4	0.0	3.9084428597556684	0.0
52-53	6.818146565264919E-5	2.3863512978427215E-4	0.0	4.364653569120751	0.0
54-55	6.818146565264919E-5	2.3863512978427215E-4	0.0	4.855713530117543	0.0
56-57	6.818146565264919E-5	2.3863512978427215E-4	0.0	5.411946449595062	0.0
58-59	6.818146565264919E-5	2.3863512978427215E-4	0.0	5.986605410165209	0.0
60-61	7.670414885923034E-5	2.3863512978427215E-4	0.0	6.542684921345009	0.0
62-63	9.374951527239262E-5	2.3863512978427215E-4	0.0	7.070827076973636	0.0
64-65	1.0227219847897377E-4	2.3863512978427215E-4	0.0	7.614344153106936	0.0
66-67	1.0227219847897377E-4	2.3863512978427215E-4	0.0	8.200892256750263	0.0
68-69	1.1931756489213608E-4	2.3863512978427215E-4	0.0	8.821940182013831	0.0
70-71	1.1931756489213608E-4	2.3863512978427215E-4	0.0	9.439485284479495	0.0
72-73	1.1931756489213608E-4	2.3863512978427215E-4	0.0	10.040504904207598	0.0
74-75	1.1931756489213608E-4	2.3863512978427215E-4	0.0	10.626754713938695	0.0
76-77	1.1931756489213608E-4	2.556804961974344E-4	0.0	11.232615217728135	0.0
78-79	1.2784024809871724E-4	2.556804961974344E-4	0.0	11.88388457764224	0.0
80-81	1.5340829771846065E-4	2.7272586261059675E-4	0.0	12.54760557772116	0.0
82-83	1.5340829771846065E-4	2.7272586261059675E-4	0.0	13.193249966718923	0.0
84-85	1.5340829771846065E-4	2.7272586261059675E-4	0.0	13.81693992377653	0.0
86-87	1.5340829771846065E-4	2.7272586261059675E-4	0.0	14.452229252678297	0.0
88	1.5340829771846065E-4	2.7272586261059675E-4	0.0	14.94645112914473	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9810	0.0	60.04695	1
GTATCAA	17915	0.0	39.546253	1
ATCAACG	21965	0.0	32.008743	3
TCAACGC	22035	0.0	31.821743	4
CAACGCA	22675	0.0	30.944304	5
TATCAAC	23185	0.0	30.425787	2
GTGGTAT	4320	0.0	30.253101	1
AACGCAG	23505	0.0	29.882683	6
TGGTATC	4130	0.0	29.586357	2
ACGCAGA	26590	0.0	26.221262	7
CGCAGAG	26865	0.0	25.707684	8
GCAGAGT	29355	0.0	23.109978	9
GTACATG	14155	0.0	22.05297	1
GAGTACT	21055	0.0	21.963902	12-13
CAGAGTA	28675	0.0	21.074001	10-11
AGAGTAC	26895	0.0	20.623318	10-11
TACATGG	14530	0.0	20.183067	2
GTACTTT	22935	0.0	19.978674	14-15
ACATGGG	14500	0.0	19.284891	3
AGTACTT	22235	0.0	19.233395	12-13
>>END_MODULE