FastQCFastQC Report
Wed 25 May 2016
SRR2049366_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049366_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7719376
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72250.043.791431
GTATCAA123000.036.57791
TCAACGC132800.032.992324
ATCAACG135600.032.3110663
CAACGCA139350.031.5437835
AACGCAG149300.029.3417786
TATCAAC154300.028.456142
ACGCAGA176200.024.3014137
GTACATG203950.024.1573621
CGCAGAG178750.023.7180828
TACATGG204300.022.6193542
ACATGGG210500.021.2384823
GCAGAGT203450.019.72999
GTGGTAT50050.018.0347061
CATGGGG151550.017.743334
AGAGTAC174550.017.28780410-11
CAGAGTA189500.017.16412210-11
GAGTACA156950.017.1035671
GTATTAG34100.015.7167381
TGGTATC52100.015.068632
Produced by FastQC (version 0.11.5)