##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049366_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7719376
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.40339232083008	31.0	31.0	34.0	27.0	34.0
2	30.493893936504712	31.0	31.0	34.0	27.0	34.0
3	30.690546878400532	31.0	31.0	34.0	27.0	34.0
4	34.28469127556424	37.0	35.0	37.0	30.0	37.0
5	34.317334458122005	37.0	35.0	37.0	30.0	37.0
6	34.22684735139213	37.0	35.0	37.0	30.0	37.0
7	34.2076802580934	37.0	35.0	37.0	30.0	37.0
8	34.19340565869573	37.0	35.0	37.0	30.0	37.0
9	35.7424295694367	39.0	35.0	39.0	30.0	39.0
10-11	35.69738389476041	39.0	35.0	39.0	30.0	39.0
12-13	35.63037750719747	39.0	35.0	39.0	30.0	39.0
14-15	36.75718231629085	40.0	37.0	41.0	30.0	41.0
16-17	36.70624523277529	40.0	37.0	41.0	30.0	41.0
18-19	36.718597526535824	40.0	37.0	41.0	30.0	41.0
20-21	36.80634924377307	40.0	37.0	41.0	30.0	41.0
22-23	36.735514502726645	40.0	37.0	41.0	30.0	41.0
24-25	36.740238071056524	40.0	37.0	41.0	30.0	41.0
26-27	36.73037626357363	40.0	37.0	41.0	30.0	41.0
28-29	36.66057858821749	40.0	37.0	41.0	30.0	41.0
30-31	36.58805957890897	40.0	37.0	41.0	30.0	41.0
32-33	36.539109443561244	40.0	36.0	41.0	30.0	41.0
34-35	36.43272921282757	40.0	36.0	41.0	30.0	41.0
36-37	36.332879432223535	40.0	36.0	41.0	30.0	41.0
38-39	36.216079253038075	40.0	36.0	41.0	29.0	41.0
40-41	36.10899533848332	40.0	36.0	41.0	29.0	41.0
42-43	35.947678542928855	39.5	35.5	41.0	28.5	41.0
44-45	35.793531562654806	39.0	35.0	41.0	28.0	41.0
46-47	35.66481390464722	39.0	35.0	41.0	28.0	41.0
48-49	35.527943515123496	39.0	35.0	41.0	27.0	41.0
50-51	35.382996501271606	39.0	35.0	40.0	27.0	41.0
52-53	35.230132267167704	39.0	35.0	40.0	26.5	41.0
54-55	35.08041828769579	39.0	34.5	40.0	26.0	41.0
56-57	34.944098266492006	38.5	34.0	40.0	26.0	41.0
58-59	34.73986388278016	38.0	34.0	40.0	26.0	41.0
60-61	34.51202713794483	38.0	34.0	40.0	25.5	41.0
62-63	34.28854858216519	38.0	34.0	40.0	25.0	41.0
64-65	34.038742056352746	37.0	33.5	40.0	24.5	41.0
66-67	33.77329223242915	37.0	33.0	40.0	23.5	41.0
68-69	33.47843342518877	36.0	33.0	39.5	23.0	41.0
70-71	33.182494802688716	36.0	33.0	39.0	23.0	41.0
72-73	32.83361589071448	35.5	32.5	39.0	22.0	40.5
74-75	32.49003112427741	35.0	32.0	38.5	21.5	40.0
76-77	32.1553115821797	35.0	32.0	37.5	20.0	39.5
78-79	31.832948544027396	35.0	32.0	37.0	20.0	39.0
80-81	31.477361771210525	35.0	32.0	36.5	18.5	39.0
82-83	31.16000781669399	35.0	31.5	36.0	18.0	38.5
84-85	30.861410883470374	35.0	31.0	36.0	14.5	37.0
86-87	30.53621568634563	35.0	31.0	35.0	9.0	37.0
88-89	30.274040285121494	34.5	31.0	35.0	7.0	36.5
90-91	30.0518166882919	34.0	31.0	35.0	2.0	36.0
92-93	29.79095454088517	34.0	31.0	35.0	2.0	36.0
94-95	29.561087644908085	34.0	31.0	35.0	2.0	36.0
96-97	29.269927452685295	34.0	30.5	35.0	2.0	35.0
98-99	29.03187129374188	34.0	30.0	35.0	2.0	35.0
100	28.88479029911226	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5734994635850832
1101	2	0.5066711575020513
1101	3	0.5287164507917801
1101	4	0.5629778928668969
1101	5	0.5458687129093178
1101	6	0.6248035859677046
1101	7	0.6174804264905873
1101	8	0.680440646297555
1101	9	0.854434629077339
1101	10-11	0.7784576696548413
1101	12-13	0.8232044366489575
1101	14-15	1.0123548404192206
1101	16-17	0.9682492917908263
1101	18-19	0.9327284486045855
1101	20-21	0.9068359978160103
1101	22-23	0.926164580437252
1101	24-25	0.9577416725959651
1101	26-27	0.8486374119986877
1101	28-29	0.8921478097858326
1101	30-31	0.8982550432480565
1101	32-33	0.8285174002564588
1101	34-35	0.882154651580116
1101	36-37	0.8779680005675914
1101	38-39	0.9800328663863809
1101	40-41	0.9951626779477891
1101	42-43	0.9400764719785712
1101	44-45	0.8596351686857417
1101	46-47	0.8851173360390874
1101	48-49	0.7624546639533349
1101	50-51	0.8477514454634587
1101	52-53	0.8246712974123724
1101	54-55	0.8327025469338523
1101	56-57	0.8158946494088326
1101	58-59	0.935323590552251
1101	60-61	0.9275890652482133
1101	62-63	0.887041386672891
1101	64-65	0.8596999052846783
1101	66-67	0.8787181563824973
1101	68-69	0.8605182732216505
1101	70-71	0.8992245258709701
1101	72-73	0.9461890786295868
1101	74-75	0.9013147286099397
1101	76-77	0.9075470896914339
1101	78-79	0.8723312747666334
1101	80-81	0.8505445140062271
1101	82-83	0.8794895208361524
1101	84-85	0.830811624772263
1101	86-87	0.8565397779451516
1101	88-89	0.871485919483888
1101	90-91	0.8868713156341066
1101	92-93	0.9400158677537505
1101	94-95	0.9046226904981616
1101	96-97	0.911552007404449
1101	98-99	1.0654307960353577
1101	100	1.070483887901517
1102	1	0.2567017466356454
1102	2	0.24115155450730086
1102	3	0.32250323322562835
1102	4	0.31301621212149655
1102	5	0.260548081533436
1102	6	0.3256536932738143
1102	7	0.4412344775387709
1102	8	0.43448715609901
1102	9	0.5320087726973171
1102	10-11	0.4627241243121176
1102	12-13	0.5498086414497649
1102	14-15	0.6232773053492195
1102	16-17	0.5492980475413702
1102	18-19	0.6144709283354572
1102	20-21	0.5387616869641505
1102	22-23	0.5199366745494132
1102	24-25	0.5776922723780942
1102	26-27	0.5619768654370176
1102	28-29	0.5868105770001293
1102	30-31	0.5622691490195137
1102	32-33	0.6446260065188838
1102	34-35	0.6539771447717655
1102	36-37	0.6065280161162008
1102	38-39	0.6734211547050819
1102	40-41	0.6433897734779066
1102	42-43	0.5409840093855323
1102	44-45	0.514168647484162
1102	46-47	0.46229840372449615
1102	48-49	0.5070654688994907
1102	50-51	0.5735714252465058
1102	52-53	0.5391803243625475
1102	54-55	0.46199696027399284
1102	56-57	0.4147574943870893
1102	58-59	0.49214013907644727
1102	60-61	0.4963216562942989
1102	62-63	0.47748297657823
1102	64-65	0.4388517054917216
1102	66-67	0.48036597666587255
1102	68-69	0.5247751387446087
1102	70-71	0.5105107406153877
1102	72-73	0.4574205836876004
1102	74-75	0.37382463560503965
1102	76-77	0.44776052105794406
1102	78-79	0.47654020890389504
1102	80-81	0.4212180201589639
1102	82-83	0.4299877216691925
1102	84-85	0.42151060438774124
1102	86-87	0.43160194938955954
1102	88-89	0.46967239782365766
1102	90-91	0.4883781107816887
1102	92-93	0.492213352003656
1102	94-95	0.4793091172171273
1102	96-97	0.4762468866593359
1102	98-99	0.5147234290414993
1102	100	0.5153156248228221
1103	1	0.2790263693151047
1103	2	0.35423247826106774
1103	3	0.3382371265036319
1103	4	0.3470963572264836
1103	5	0.3598384563466013
1103	6	0.3761750012647198
1103	7	0.4322537542525495
1103	8	0.4170017003504398
1103	9	0.5548866584594521
1103	10-11	0.549153778961724
1103	12-13	0.5146108668444711
1103	14-15	0.6192332332124195
1103	16-17	0.6110272803486794
1103	18-19	0.6034131944368113
1103	20-21	0.5622592199297642
1103	22-23	0.5289545110327722
1103	24-25	0.5593735574839656
1103	26-27	0.5671645065834596
1103	28-29	0.5632396701512405
1103	30-31	0.5756786131297673
1103	32-33	0.6589339551427003
1103	34-35	0.6198683991643819
1103	36-37	0.5651274087509535
1103	38-39	0.6191413740377882
1103	40-41	0.636729903779333
1103	42-43	0.6575336720907927
1103	44-45	0.5416732511263049
1103	46-47	0.5613375774820923
1103	48-49	0.5582252372734118
1103	50-51	0.5024218714441417
1103	52-53	0.5677761255257323
1103	54-55	0.591503383058722
1103	56-57	0.519133680805119
1103	58-59	0.5875701620108131
1103	60-61	0.6165087475297497
1103	62-63	0.5810687694278514
1103	64-65	0.6052079243475532
1103	66-67	0.6807347149850216
1103	68-69	0.7231857172440002
1103	70-71	0.6233310277549862
1103	72-73	0.6376983029667542
1103	74-75	0.6297995830713248
1103	76-77	0.7230446729050186
1103	78-79	0.5821754961251138
1103	80-81	0.5516343039268001
1103	82-83	0.5473743887746387
1103	84-85	0.6149839813301305
1103	86-87	0.6151281535414768
1103	88-89	0.5327115179144464
1103	90-91	0.5866126424497224
1103	92-93	0.5646785454695227
1103	94-95	0.6402098699065384
1103	96-97	0.6242342787004027
1103	98-99	0.6018038103003107
1103	100	0.5565245993037422
1104	1	0.12446850873787696
1104	2	0.10884262141609469
1104	3	0.1735036145325246
1104	4	0.27149146321931283
1104	5	0.2726878067537726
1104	6	0.30695036963073363
1104	7	0.3811849706947257
1104	8	0.34590414824481996
1104	9	0.4856037909305897
1104	10-11	0.495315268096725
1104	12-13	0.4436173287487435
1104	14-15	0.5695343533582786
1104	16-17	0.5743159563871814
1104	18-19	0.5508191642819682
1104	20-21	0.5138427641405343
1104	22-23	0.5044744193158337
1104	24-25	0.5808393893161039
1104	26-27	0.49300136964073715
1104	28-29	0.4697477472229892
1104	30-31	0.47630369333975864
1104	32-33	0.5122409359015094
1104	34-35	0.5432517125025029
1104	36-37	0.488040758338812
1104	38-39	0.5700292009722574
1104	40-41	0.5589289889765325
1104	42-43	0.5567141372981865
1104	44-45	0.5014531417725578
1104	46-47	0.45108006561970626
1104	48-49	0.46382154559412925
1104	50-51	0.45360635392975723
1104	52-53	0.4105189875854265
1104	54-55	0.44389947564167187
1104	56-57	0.4491943720108651
1104	58-59	0.5079941885135497
1104	60-61	0.4627380425246912
1104	62-63	0.45104833230043084
1104	64-65	0.4952305061472302
1104	66-67	0.594430864207041
1104	68-69	0.5498493082922593
1104	70-71	0.5395377321445025
1104	72-73	0.5664884303542976
1104	74-75	0.6343773473990169
1104	76-77	0.6087501956609387
1104	78-79	0.5424260266775427
1104	80-81	0.6030161240924876
1104	82-83	0.49417257306324913
1104	84-85	0.5421032252098001
1104	86-87	0.5369557814724004
1104	88-89	0.49475543413190337
1104	90-91	0.5835752596046504
1104	92-93	0.571863743957099
1104	94-95	0.6020710825024054
1104	96-97	0.6001455947807592
1104	98-99	0.554859534661162
1104	100	0.5535073242352446
1105	1	0.1347467431627507
1105	2	0.12530888124003425
1105	3	0.19148077743824388
1105	4	0.22443372727573063
1105	5	0.2239927283643368
1105	6	0.20539377517533097
1105	7	0.18721782283216726
1105	8	0.3008486443007712
1105	9	0.3748951166216372
1105	10-11	0.3453067134980756
1105	12-13	0.3364601629742907
1105	14-15	0.3835538973207733
1105	16-17	0.3605285481570206
1105	18-19	0.3728812928179863
1105	20-21	0.38371605971495626
1105	22-23	0.38272502503467365
1105	24-25	0.29524506530639627
1105	26-27	0.31150587572741273
1105	28-29	0.3505895750110284
1105	30-31	0.3234315770673959
1105	32-33	0.3367597616029343
1105	34-35	0.33965713541878273
1105	36-37	0.33740730380951334
1105	38-39	0.31729444649020877
1105	40-41	0.3826349569317955
1105	42-43	0.3530430799468576
1105	44-45	0.333517405897517
1105	46-47	0.32476372341902504
1105	48-49	0.29260117657160123
1105	50-51	0.32697013155460297
1105	52-53	0.397957784312311
1105	54-55	0.3625723344572549
1105	56-57	0.31252117074094343
1105	58-59	0.29211332026223147
1105	60-61	0.2996153321304007
1105	62-63	0.28705680381293774
1105	64-65	0.3380717957455204
1105	66-67	0.3549891350843666
1105	68-69	0.3750136552427463
1105	70-71	0.3819444305556061
1105	72-73	0.3230701216414502
1105	74-75	0.39703112127642015
1105	76-77	0.41522849606651846
1105	78-79	0.2732438122841323
1105	80-81	0.3750166429189967
1105	82-83	0.39854285572072
1105	84-85	0.32169402284262105
1105	86-87	0.3618267806859521
1105	88-89	0.35092680812618227
1105	90-91	0.40565928632089054
1105	92-93	0.41459135154309124
1105	94-95	0.39418377108616554
1105	96-97	0.35183438536931533
1105	98-99	0.3518606962373845
1105	100	0.3707923258850627
1106	1	0.24667400936210981
1106	2	0.24184428028224403
1106	3	0.2736590558914287
1106	4	0.27216375680701077
1106	5	0.2495150962071122
1106	6	0.31877283397573564
1106	7	0.34644867187714823
1106	8	0.32930435752877685
1106	9	0.44361792281952006
1106	10-11	0.4234493676425046
1106	12-13	0.4550127220120075
1106	14-15	0.5365217060415262
1106	16-17	0.5271061299219326
1106	18-19	0.5171617258279966
1106	20-21	0.4751552332592439
1106	22-23	0.534808549803806
1106	24-25	0.52398480024425
1106	26-27	0.48117045150752347
1106	28-29	0.5633402113496118
1106	30-31	0.5081418058162512
1106	32-33	0.5491903509756497
1106	34-35	0.6116251776341528
1106	36-37	0.5211305879311539
1106	38-39	0.5388249346674314
1106	40-41	0.5575195427460145
1106	42-43	0.5691277698195591
1106	44-45	0.5241337158126314
1106	46-47	0.47728840105635584
1106	48-49	0.48081440061006475
1106	50-51	0.5194319858583469
1106	52-53	0.5273315720169052
1106	54-55	0.6001332463675197
1106	56-57	0.5157593308346833
1106	58-59	0.5600049891087338
1106	60-61	0.5710393868929629
1106	62-63	0.5246334436213118
1106	64-65	0.5350242856022263
1106	66-67	0.5863498313083042
1106	68-69	0.604632413383996
1106	70-71	0.5775047894786312
1106	72-73	0.5760353847687512
1106	74-75	0.607159502880485
1106	76-77	0.6529145246561683
1106	78-79	0.5134783690479487
1106	80-81	0.5503547834892792
1106	82-83	0.554576028520799
1106	84-85	0.5197375418823249
1106	86-87	0.534518536643489
1106	88-89	0.5631063054642951
1106	90-91	0.5910084242462119
1106	92-93	0.5752200379118726
1106	94-95	0.5987315966243649
1106	96-97	0.6206119359566813
1106	98-99	0.6488932798988785
1106	100	0.6557631007982785
1107	1	0.1622577398180809
1107	2	0.14400300342226302
1107	3	0.15677361228856057
1107	4	0.15433073355946192
1107	5	0.16778429814340257
1107	6	0.20161003144962564
1107	7	0.21113393320262475
1107	8	0.28004031370276294
1107	9	0.3229210656553292
1107	10-11	0.2939279632156939
1107	12-13	0.27489043066704966
1107	14-15	0.2584574467775127
1107	16-17	0.30958514714372143
1107	18-19	0.2827725220183268
1107	20-21	0.2762891433161485
1107	22-23	0.28246315385035103
1107	24-25	0.3199440901496189
1107	26-27	0.3131588384859967
1107	28-29	0.3749215945930189
1107	30-31	0.33813538847846303
1107	32-33	0.35335365046468326
1107	34-35	0.33754044325227284
1107	36-37	0.33008892340885154
1107	38-39	0.34369179541072015
1107	40-41	0.39420340436883805
1107	42-43	0.3560290066954508
1107	44-45	0.3023813270865361
1107	46-47	0.28949825090275283
1107	48-49	0.28808787459762897
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2214	96-97	-0.2502139136400423
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2214	100	-0.18509797216531254
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2215	4	-0.3101827978451084
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2215	24-25	-0.6990559550555915
2215	26-27	-0.6320322452988663
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2215	100	-0.41844474169072043
2216	1	-1.2281420070776292
2216	2	-1.1362722513730965
2216	3	-1.221257331266024
2216	4	-1.4251786726294853
2216	5	-1.3872830829249025
2216	6	-2.7956800301076328
2216	7	-2.6477073433796967
2216	8	-3.565987335448419
2216	9	-3.0983827583542265
2216	10-11	-2.1973810455451286
2216	12-13	-2.0204529904339594
2216	14-15	-2.2755438871341838
2216	16-17	-2.6130029021390015
2216	18-19	-2.301625026155328
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2216	26-27	-2.400991799617451
2216	28-29	-2.9025165537003303
2216	30-31	-2.5257856320247782
2216	32-33	-2.678532875279359
2216	34-35	-3.583083183103426
2216	36-37	-2.5964033243595566
2216	38-39	-2.5646766951291013
2216	40-41	-3.1543148665646896
2216	42-43	-2.8979123838390066
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2216	50-51	-2.911254492753386
2216	52-53	-3.2400262580256225
2216	54-55	-2.6129637203940845
2216	56-57	-2.5791010749707226
2216	58-59	-3.567589606006152
2216	60-61	-4.260306800071852
2216	62-63	-3.3971564789336313
2216	64-65	-3.4113469853949
2216	66-67	-3.041534424232669
2216	68-69	-3.4741840328326035
2216	70-71	-3.248747869760166
2216	72-73	-3.674611257262839
2216	74-75	-4.163319382986771
2216	76-77	-4.762750289935184
2216	78-79	-3.841275751258486
2216	80-81	-3.647046915405852
2216	82-83	-3.821104746761236
2216	84-85	-3.8648445087358567
2216	86-87	-3.561264568883118
2216	88-89	-3.4967224354056654
2216	90-91	-3.616628050515722
2216	92-93	-3.750981357788614
2216	94-95	-3.611843600500997
2216	96-97	-3.816003243592146
2216	98-99	-3.59978574508013
2216	100	-3.1789212031929885
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	154180.0
3	25882.0
4	17440.0
5	14032.0
6	13059.0
7	13521.0
8	14860.0
9	17268.0
10	19486.0
11	21450.0
12	23856.0
13	26170.0
14	28556.0
15	29679.0
16	29745.0
17	30684.0
18	29170.0
19	27960.0
20	28230.0
21	29332.0
22	32736.0
23	38852.0
24	47093.0
25	58408.0
26	71752.0
27	84250.0
28	102090.0
29	126560.0
30	151458.0
31	181369.0
32	230211.0
33	302092.0
34	409490.0
35	587944.0
36	940173.0
37	1516919.0
38	1731769.0
39	510835.0
40	815.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.10051914207655	20.308419808427	14.255709571692563	23.335351477803886
2	15.433531285013357	20.230336861699136	40.43824184903958	23.89789000424793
3	18.197144053450476	25.127760266469657	30.513745624530802	26.16135005554907
4	12.715816145495715	16.814032335779117	38.74641899350553	31.723732525219646
5	13.109581219047096	37.60537303668484	35.69350186971288	13.591543874555187
6	30.892086741167063	37.7293492079743	17.70817322832605	13.67039082253259
7	26.815454294632886	32.505241719094656	22.766510588991814	17.912793397280645
8	24.748263102558692	36.32355336734103	20.708278078281456	18.21990545181883
9	24.749176015396685	17.061979271083516	21.774369021470577	36.41447569204922
10-11	23.778829601398417	27.000995731256623	29.05362635189455	20.16654831545041
12-13	24.107010542883497	24.57819232363001	29.03291941745717	22.281877716029324
14-15	21.65993324680862	26.8369212147759	26.11185662902215	25.391288909393335
16-17	21.279505967801963	30.092678984815358	26.824804606709773	21.803010440672907
18-19	21.196655174867107	29.101115179296567	28.730371487550666	20.971858158285663
20-21	22.27517030226108	28.337044393016903	28.287378569816084	21.10040673490593
22-23	21.98829895533468	28.158169831220555	28.31008474642715	21.543446467017617
24-25	21.818882302984804	28.294290582926052	28.245079346073048	21.641747768016092
26-27	21.52868467331124	28.273754197943155	28.300890060168655	21.89667106857695
28-29	21.616724373839325	28.26545632035603	28.46903455166882	21.648784754135825
30-31	21.859719796741707	28.613151711279095	28.04140353550826	21.48572495647094
32-33	21.534151002408223	28.387010158433835	28.398252022903858	21.680586816254092
34-35	21.728825009280193	28.126680000080345	28.41291621480597	21.731578775833487
36-37	21.988397531778496	28.37313724403962	27.945886248901353	21.69257897528053
38-39	21.80674099178792	28.519346887332368	28.07528682483157	21.59862529604814
40-41	21.725785148010292	28.273343374225835	28.266838068092543	21.73403340967133
42-43	21.591840648767167	28.300135159826816	28.224792951473344	21.883231239932666
44-45	21.734900005708276	28.362001385147895	28.14151030622472	21.761588302919105
46-47	21.732380459525615	28.439167537532544	28.008135093505043	21.820316909436794
48-49	21.658191793886946	28.303580568956825	28.091831855414007	21.94639578174222
50-51	21.680235801198148	28.249051959678575	28.057937449651504	22.012774789471777
52-53	21.653332141130857	28.252146936363605	28.09779559170175	21.99672533080379
54-55	21.746111760482894	28.513637671280804	27.975750499555517	21.764500068680785
56-57	21.646598478951905	28.303903540522935	27.989382895371723	22.060115085153438
58-59	21.54431989558526	27.94500343271757	28.25674057959408	22.253936092103093
60-61	21.74333264091914	28.273273637122177	27.961006930059646	22.02238679189904
62-63	21.628921626051323	28.338286929865575	27.94119362152311	22.091597822559994
64-65	21.65553753937503	28.068116453031738	28.075852482598147	22.200493524995085
66-67	21.54750722068184	28.07604156448517	27.972913032136944	22.403538182696046
68-69	21.841034048027144	28.20068547265256	27.881928637914342	22.076351841405952
70-71	21.762318043535885	28.008675480241703	27.988810045560264	22.24019643066215
72-73	21.56885396513989	28.098833374583553	28.115024627031033	22.21728803324552
74-75	21.612444922689292	28.131947632707437	28.020539303135227	22.23506814146804
76-77	21.696670493045687	28.08763485653541	27.885199817779956	22.33049483263895
78-79	21.711416138240438	28.083825378933586	28.017303573912645	22.18745490891333
80-81	21.66685164764926	27.979569028650282	28.049557279928187	22.304022043772267
82-83	21.52973856338309	28.0017148907891	28.167100648241416	22.301445897586394
84-85	21.605559392667907	27.887438561208132	28.058864615013807	22.448137431110155
86-87	21.732323890715016	28.00987508354933	27.976403518570304	22.28139750716535
88-89	21.624751440495064	27.822913541787475	28.069642663922927	22.48269235379453
90-91	21.58919768436652	27.753909646546337	28.104398402578845	22.552494266508297
92-93	21.715653630991003	27.894897184197127	27.937614268037294	22.451834916774576
94-95	21.6155989038419	27.676262099578818	28.091827700118664	22.616311296460616
96-97	21.661861010527275	27.628509091926603	28.086661408901442	22.62296848864468
98-99	21.6535248444952	27.670688667063242	28.005463135880415	22.67032335256114
100	21.736381282632173	27.559494446183212	27.99679663226665	22.707327638917967
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1858.0
1	1533.5
2	1519.0
3	1819.5
4	1712.5
5	1480.0
6	1211.0
7	1029.0
8	1021.5
9	1054.0
10	974.5
11	903.0
12	939.0
13	1027.0
14	1147.5
15	1364.0
16	1659.5
17	2087.0
18	2751.5
19	3805.0
20	5536.5
21	7995.5
22	11773.5
23	17770.0
24	25738.5
25	35827.0
26	50010.0
27	67269.5
28	87484.5
29	111040.5
30	134793.0
31	155656.0
32	173335.5
33	191153.0
34	210546.5
35	226336.5
36	239001.0
37	253801.5
38	267384.0
39	274856.0
40	279907.5
41	282736.0
42	282836.5
43	283058.5
44	280319.0
45	276187.5
46	270875.0
47	261916.5
48	250712.0
49	241332.5
50	235625.5
51	226805.0
52	212106.0
53	199103.5
54	188201.5
55	177836.5
56	165138.0
57	150452.5
58	137621.5
59	125397.5
60	111429.5
61	95524.5
62	81762.5
63	69192.0
64	56955.0
65	46432.5
66	37112.0
67	28714.5
68	22183.5
69	17245.5
70	13008.5
71	9766.5
72	7354.5
73	5175.0
74	3599.0
75	2537.5
76	1699.0
77	1149.5
78	783.5
79	564.0
80	378.0
81	264.0
82	209.5
83	147.5
84	114.5
85	90.0
86	69.0
87	61.5
88	52.0
89	43.0
90	34.0
91	26.5
92	25.0
93	24.0
94	18.5
95	15.0
96	17.5
97	19.0
98	21.0
99	28.0
100	51.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013796451941193173
2	0.03469192328499091
3	0.035948501536911794
4	0.03847461245572181
5	0.041687307367849424
6	0.039977324592039565
7	0.03781393729234073
8	0.03739939601335652
9	0.03768439314265816
10-11	0.038040639554285216
12-13	0.0392972178062061
14-15	0.037451213673229546
16-17	0.03409602019645111
18-19	0.03703667239424534
20-21	0.03629827074105472
22-23	0.03737996439090414
24-25	0.03423204155361781
26-27	0.034335676873363856
28-29	0.03480203581222109
30-31	0.0348732850945465
32-33	0.03477612698228458
34-35	0.034620674002665504
36-37	0.03217228957366502
38-39	0.032573876437680976
40-41	0.03366204729501452
42-43	0.033500117107911315
44-45	0.03252205877780795
46-47	0.031602295315061736
48-49	0.0316864990123554
50-51	0.03285887356698262
52-53	0.03324102880854619
54-55	0.03259330806013336
56-57	0.031019346641490194
58-59	0.03184195199197448
60-61	0.03128491214833945
62-63	0.03140150188305376
64-65	0.02900493511392631
66-67	0.02988583533176775
68-69	0.030637191399926624
70-71	0.03179661153958558
72-73	0.029095616018704102
74-75	0.029393567562974003
76-77	0.03021617291345829
78-79	0.030540033287664702
80-81	0.03065662302237901
82-83	0.029361181525553358
84-85	0.030391057515529753
86-87	0.03080559879451396
88-89	0.031233094488466426
90-91	0.03158286369260935
92-93	0.030093105971259852
94-95	0.03139502467556963
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7719376.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.24151165819931
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.8608073021666	43.60006581231349
2	14.502519574419537	16.892973257336383
3	4.705744850528475	8.222090807176968
4	2.027133567853294	4.7225329329941905
5	1.0756840357061639	3.1324732153059713
6	0.660046759574387	2.3065272625625
7	0.41523719852779767	1.6928829497282325
8	0.294473943238005	1.3720486078505754
9	0.21142803384084746	1.1082499468030687
>10	1.155640268138977	12.024187463735172
>50	0.06916509847330611	2.747978956341596
>100	0.02187435197647861	2.057980258863
>500	1.782182246178041E-4	0.058138567163602675
>1k	6.679733163053334E-5	0.061869961825267314
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.2954414968256502E-5	0.0
4	0.0	0.0	0.0	3.88632449047695E-5	0.0
5	0.0	0.0	0.0	3.88632449047695E-5	0.0
6	0.0	0.0	0.0	3.88632449047695E-5	0.0
7	0.0	0.0	0.0	5.181765987302601E-5	0.0
8	0.0	0.0	0.0	6.477207484128251E-5	0.0
9	0.0	0.0	0.0	1.1658973471430851E-4	0.0
10-11	0.0	0.0	0.0	1.2306694219843677E-4	0.0
12-13	6.477207484128251E-6	6.477207484128251E-6	0.0	1.3602135716669325E-4	0.0
14-15	1.2954414968256502E-5	1.2954414968256502E-5	0.0	1.8783901703971927E-4	0.0
16-17	1.2954414968256502E-5	1.943162245238475E-5	0.0	2.0727063949210403E-4	0.0
18-19	1.2954414968256502E-5	2.5908829936513004E-5	0.0	2.4613388439687355E-4	0.0
20-21	1.2954414968256502E-5	2.5908829936513004E-5	0.0	3.173831667222843E-4	0.0
22-23	1.2954414968256502E-5	2.5908829936513004E-5	0.0	5.311310136985165E-4	0.0
24-25	1.2954414968256502E-5	4.5340452388897755E-5	0.0	9.845355375874942E-4	0.0
26-27	2.5908829936513004E-5	5.181765987302601E-5	0.0	0.001729414398262243	0.0
28-29	2.5908829936513004E-5	5.181765987302601E-5	0.0	0.005324264551953422	0.0
30-31	2.5908829936513004E-5	5.181765987302601E-5	0.0	0.016788921798860428	0.0
32-33	2.5908829936513004E-5	5.181765987302601E-5	0.0	0.04274309218776233	0.0
34-35	2.5908829936513004E-5	5.181765987302601E-5	0.0	0.0859784521443184	0.0
36-37	2.5908829936513004E-5	6.47720748412825E-5	0.0	0.15306289005743468	0.0
38-39	2.5908829936513004E-5	8.420369729366726E-5	0.0	0.24231233198123786	0.0
40-41	2.5908829936513004E-5	9.068090477779551E-5	0.0	0.33419799735108124	0.0
42-43	2.5908829936513004E-5	9.068090477779551E-5	0.0	0.4260447994760198	0.0
44-45	2.5908829936513004E-5	1.1011252723018027E-4	0.0	0.5384955986079705	0.0
46-47	2.5908829936513004E-5	1.2954414968256503E-4	0.0	0.673519465822108	0.0
48-49	2.5908829936513004E-5	1.2954414968256503E-4	0.0	0.8104670636590315	0.0
50-51	2.5908829936513004E-5	1.6840739458733452E-4	0.0	0.9466244421828915	0.0
52-53	2.5908829936513004E-5	1.6840739458733452E-4	0.0	1.0768810846887105	0.0
54-55	2.5908829936513004E-5	1.6840739458733452E-4	0.0	1.222644161911533	0.0
56-57	2.5908829936513004E-5	1.6840739458733452E-4	0.0	1.39493140378186	0.0
58-59	2.5908829936513004E-5	1.6840739458733452E-4	0.0	1.5803414680150314	0.0
60-61	2.5908829936513004E-5	1.6840739458733452E-4	0.0	1.7670145877076076	0.0
62-63	2.5908829936513004E-5	1.6840739458733452E-4	0.0	1.9466534600724203	0.0
64-65	2.5908829936513004E-5	1.6840739458733452E-4	0.0	2.1381780081706085	0.0
66-67	2.5908829936513004E-5	1.6840739458733452E-4	0.0	2.3557279759400243	0.0
68-69	2.5908829936513004E-5	1.6840739458733452E-4	0.0	2.5934997854748882	0.0
70-71	2.5908829936513004E-5	1.8136180955559102E-4	0.0	2.841777625549008	0.0
72-73	2.5908829936513004E-5	1.8136180955559102E-4	0.0	3.0970508497059868	0.0
74-75	2.5908829936513004E-5	1.8136180955559102E-4	0.0	3.3508731793865203	0.0
76-77	2.5908829936513004E-5	2.0727063949210403E-4	0.0	3.624088268274534	0.0
78-79	3.88632449047695E-5	2.0727063949210403E-4	0.0	3.925906705412458	0.0
80-81	3.88632449047695E-5	2.1374784697623228E-4	0.0	4.245712347733806	6.477207484128251E-6
82-83	3.88632449047695E-5	2.2022505446036054E-4	0.0	4.569488518242926	1.2954414968256502E-5
84-85	3.88632449047695E-5	2.2022505446036054E-4	0.0	4.890848690360464	1.2954414968256502E-5
86-87	3.88632449047695E-5	2.3317946942861702E-4	0.0	5.23002766026684	1.2954414968256502E-5
88	3.88632449047695E-5	2.3317946942861702E-4	0.0	5.497426216834107	1.2954414968256502E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7225	0.0	43.79143	1
GTATCAA	12300	0.0	36.5779	1
TCAACGC	13280	0.0	32.99232	4
ATCAACG	13560	0.0	32.311066	3
CAACGCA	13935	0.0	31.543783	5
AACGCAG	14930	0.0	29.341778	6
TATCAAC	15430	0.0	28.45614	2
ACGCAGA	17620	0.0	24.301413	7
GTACATG	20395	0.0	24.157362	1
CGCAGAG	17875	0.0	23.718082	8
TACATGG	20430	0.0	22.619354	2
ACATGGG	21050	0.0	21.238482	3
GCAGAGT	20345	0.0	19.7299	9
GTGGTAT	5005	0.0	18.034706	1
CATGGGG	15155	0.0	17.74333	4
AGAGTAC	17455	0.0	17.287804	10-11
CAGAGTA	18950	0.0	17.164122	10-11
GAGTACA	15695	0.0	17.103567	1
GTATTAG	3410	0.0	15.716738	1
TGGTATC	5210	0.0	15.06863	2
>>END_MODULE