FastQCFastQC Report
Wed 25 May 2016
SRR2049366_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049366_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7719376
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161110.2087085795535805No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160270.20762040869624696No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89780.11630473758500687No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125050.055.98171
GTATCAA221150.039.1382981
TCAACGC268650.031.837754
ATCAACG270500.031.6200053
CAACGCA277000.030.9628495
TATCAAC284900.030.1702612
GTGGTAT57250.029.7281681
AACGCAG291050.029.5741846
TGGTATC60350.026.9436652
ACGCAGA338050.025.2815257
CGCAGAG342200.024.878718
GTACATG201500.024.2890641
TACATGG204200.022.6693122
GCAGAGT375400.022.2273989
ACATGGG209600.021.1659893
GAGTACT261350.020.885912-13
CAGAGTA361850.020.34950310-11
TATAACG5100.020.2726422
AGAGTAC345550.019.69640410-11
GTACTTT283500.019.12992914-15
Produced by FastQC (version 0.11.5)