##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049366_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7719376
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80510859945156	31.0	31.0	34.0	30.0	34.0
2	31.86906273253175	31.0	31.0	34.0	30.0	34.0
3	31.87334351895801	33.0	31.0	34.0	30.0	34.0
4	35.641310903886534	37.0	35.0	37.0	33.0	37.0
5	35.75531351238753	37.0	35.0	37.0	35.0	37.0
6	35.830352349723604	37.0	35.0	37.0	35.0	37.0
7	35.76629522904442	37.0	35.0	37.0	33.0	37.0
8	35.765392176776984	37.0	35.0	37.0	33.0	37.0
9	37.49238409944016	39.0	37.0	39.0	35.0	39.0
10-11	37.461418578392866	39.0	37.0	39.0	34.5	39.0
12-13	37.42175572222418	39.0	37.0	39.0	34.0	39.0
14-15	38.71402533054486	40.0	38.0	41.0	34.0	41.0
16-17	38.741840467416026	40.0	38.0	41.0	34.5	41.0
18-19	38.80462954518603	40.0	38.0	41.0	35.0	41.0
20-21	38.83119425715239	40.0	38.0	41.0	35.0	41.0
22-23	38.80255282292248	40.0	38.0	41.0	35.0	41.0
24-25	38.786902270338956	40.0	38.0	41.0	35.0	41.0
26-27	38.67806944499141	40.0	38.0	41.0	34.0	41.0
28-29	38.59301678011279	40.0	38.0	41.0	34.0	41.0
30-31	38.49054528500749	40.0	38.0	41.0	34.0	41.0
32-33	38.359324445395586	40.0	38.0	41.0	34.0	41.0
34-35	38.26025672282319	40.0	38.0	41.0	34.0	41.0
36-37	38.15009956763345	40.0	38.0	41.0	34.0	41.0
38-39	38.0542174652459	40.0	38.0	41.0	33.0	41.0
40-41	37.94339393495018	40.0	37.5	41.0	33.0	41.0
42-43	37.80966298312195	40.0	37.0	41.0	33.0	41.0
44-45	37.64233980829539	40.0	37.0	41.0	33.0	41.0
46-47	37.42172340095883	40.0	37.0	41.0	32.5	41.0
48-49	37.2193995343665	40.0	37.0	41.0	32.0	41.0
50-51	37.071910151805014	40.0	37.0	41.0	31.0	41.0
52-53	36.933182617351456	40.0	36.0	41.0	31.0	41.0
54-55	36.794008673758086	39.5	36.0	41.0	31.0	41.0
56-57	36.65066366763324	39.0	35.5	41.0	31.0	41.0
58-59	36.459422885994925	39.0	35.0	41.0	31.0	41.0
60-61	36.27610340524934	39.0	35.0	41.0	30.5	41.0
62-63	36.07338371650766	39.0	35.0	41.0	30.0	41.0
64-65	35.84335327104159	38.0	35.0	40.0	30.0	41.0
66-67	35.57462980168345	37.5	35.0	40.0	29.5	41.0
68-69	35.28203464891463	37.0	35.0	40.0	29.0	41.0
70-71	34.947940286883295	37.0	34.0	39.5	29.0	41.0
72-73	34.650777925573266	36.0	34.0	39.0	29.0	41.0
74-75	34.34458653134658	36.0	34.0	39.0	29.0	40.5
76-77	34.0153442454416	35.0	34.0	38.5	29.0	40.0
78-79	33.67708348446817	35.0	34.0	37.0	28.5	39.0
80-81	33.35916944582049	35.0	34.0	37.0	28.0	39.0
82-83	33.062025026375196	35.0	34.0	36.0	28.0	39.0
84-85	32.79418919353067	35.0	33.0	36.0	27.0	37.0
86-87	32.56379796760774	35.0	33.0	36.0	27.0	37.0
88-89	32.337812020038925	35.0	33.0	35.0	27.0	37.0
90-91	32.14354605864516	35.0	33.0	35.0	26.5	36.0
92-93	31.963442640959578	35.0	33.0	35.0	26.0	36.0
94-95	31.805686754991594	35.0	33.0	35.0	26.0	36.0
96-97	31.608504042295646	35.0	33.0	35.0	25.5	36.0
98-99	31.45627263136295	35.0	33.0	35.0	25.0	35.0
100	31.3427407863019	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.16219016094661143
1101	2	0.211925265124826
1101	3	0.4112414854872668
1101	4	0.23240392089452655
1101	5	0.1854522323712402
1101	6	0.18117500011209842
1101	7	0.09503902506773443
1101	8	0.17971020363575008
1101	9	0.26907171857845924
1101	10-11	0.2825722784307416
1101	12-13	0.2312609310211755
1101	14-15	0.3500638806046439
1101	16-17	0.2685606225385584
1101	18-19	0.34727409607062043
1101	20-21	0.31734942486914264
1101	22-23	0.3120704205567577
1101	24-25	0.340757022805974
1101	26-27	0.41675151807429955
1101	28-29	0.41490264366277785
1101	30-31	0.39552216976210985
1101	32-33	0.4584128756402279
1101	34-35	0.4366515482299249
1101	36-37	0.49033922313839895
1101	38-39	0.4358609129666604
1101	40-41	0.42926646606827035
1101	42-43	0.46261248545991407
1101	44-45	0.5186969040778422
1101	46-47	0.5307851608137923
1101	48-49	0.5953933946550976
1101	50-51	0.4438614187430403
1101	52-53	0.512043564422143
1101	54-55	0.49419643920072076
1101	56-57	0.5101118309837247
1101	58-59	0.5298350054284953
1101	60-61	0.5445543488019169
1101	62-63	0.5159775704077347
1101	64-65	0.42691322439427637
1101	66-67	0.4170742206619238
1101	68-69	0.48300114682296424
1101	70-71	0.45646687417328025
1101	72-73	0.40047251019544916
1101	74-75	0.4779780085670211
1101	76-77	0.4341898024468023
1101	78-79	0.49470995491863334
1101	80-81	0.5014659744448267
1101	82-83	0.4687806668410488
1101	84-85	0.4655908008905598
1101	86-87	0.41383364189115923
1101	88-89	0.45684183790599775
1101	90-91	0.49797001205381264
1101	92-93	0.5124928884592492
1101	94-95	0.5466094404585249
1101	96-97	0.5351975059668312
1101	98-99	0.5971648323165191
1101	100	0.64307425514016
1102	1	0.22652585489934296
1102	2	0.20171831013917796
1102	3	0.3782759943497567
1102	4	0.22149478015908386
1102	5	0.17567290423887272
1102	6	0.17966169008146693
1102	7	0.1665008808499806
1102	8	0.21029281031441371
1102	9	0.12345646919110465
1102	10-11	0.22128855326190688
1102	12-13	0.19177579633095831
1102	14-15	0.27641454765584683
1102	16-17	0.21527343067493376
1102	18-19	0.24830638055220788
1102	20-21	0.25093593706296247
1102	22-23	0.29850474239967895
1102	24-25	0.28110669615240624
1102	26-27	0.33041599004681643
1102	28-29	0.32297471267709454
1102	30-31	0.25711267806445903
1102	32-33	0.3023109685835266
1102	34-35	0.33397503148518837
1102	36-37	0.3301621996805366
1102	38-39	0.2974907422772475
1102	40-41	0.3216452454277956
1102	42-43	0.37096092088274446
1102	44-45	0.31387710840840555
1102	46-47	0.30177022280282984
1102	48-49	0.247328206904335
1102	50-51	0.31752004448129867
1102	52-53	0.3292438121183494
1102	54-55	0.21065216529597564
1102	56-57	0.23189588035488384
1102	58-59	0.21181476575397085
1102	60-61	0.26242931590519447
1102	62-63	0.22885394798014858
1102	64-65	0.20561645412816176
1102	66-67	0.20722751196620948
1102	68-69	0.23587584441096254
1102	70-71	0.17838617770815546
1102	72-73	0.21693815452287168
1102	74-75	0.18621934607224944
1102	76-77	0.18728360097850327
1102	78-79	0.19332149562235657
1102	80-81	0.17242142720942155
1102	82-83	0.22779653238877273
1102	84-85	0.1866772246687347
1102	86-87	0.21227636619973822
1102	88-89	0.21266798483290472
1102	90-91	0.27496423264958025
1102	92-93	0.31679935594453923
1102	94-95	0.22959729160845654
1102	96-97	0.3015084028757151
1102	98-99	0.33282774287740935
1102	100	0.3513403620930937
1103	1	0.17945156338315726
1103	2	0.19041787960291856
1103	3	0.33511203006570156
1103	4	0.21317106971604716
1103	5	0.1649761094252966
1103	6	0.1496847714442282
1103	7	0.1598464862584308
1103	8	0.15971718164714588
1103	9	0.23011977293640484
1103	10-11	0.22063908058878923
1103	12-13	0.2514713186353106
1103	14-15	0.2864473233101492
1103	16-17	0.2789222864942644
1103	18-19	0.25046358513587563
1103	20-21	0.29311785834067194
1103	22-23	0.24291442058124346
1103	24-25	0.26779094617563715
1103	26-27	0.27908533460226437
1103	28-29	0.3188225578719539
1103	30-31	0.2887831677325323
1103	32-33	0.32750891335994936
1103	34-35	0.35126609817309884
1103	36-37	0.3037520422056943
1103	38-39	0.2884523255163032
1103	40-41	0.31916258028706324
1103	42-43	0.3349316956139461
1103	44-45	0.416080538124703
1103	46-47	0.3722201133818004
1103	48-49	0.3073861208731685
1103	50-51	0.3278642846140727
1103	52-53	0.33797227491787396
1103	54-55	0.3193216633034055
1103	56-57	0.2502026600950771
1103	58-59	0.3829231995575242
1103	60-61	0.3558649804097058
1103	62-63	0.33482462378697164
1103	64-65	0.33946392497965405
1103	66-67	0.26429176735877036
1103	68-69	0.3048052029992334
1103	70-71	0.36280902064593334
1103	72-73	0.3231896416579474
1103	74-75	0.302495750034538
1103	76-77	0.25935281964042645
1103	78-79	0.2498904462647502
1103	80-81	0.28291512079657366
1103	82-83	0.2962963082032104
1103	84-85	0.3193604841569524
1103	86-87	0.32357811605884734
1103	88-89	0.4102071563888714
1103	90-91	0.35667429951988083
1103	92-93	0.4266206501065568
1103	94-95	0.3994667331084756
1103	96-97	0.4751645988974893
1103	98-99	0.488739903322692
1103	100	0.467329109808734
1104	1	0.14659765788646695
1104	2	0.17961672107735183
1104	3	0.3130770911547742
1104	4	0.1831860073370848
1104	5	0.12803825357219267
1104	6	0.16362404039245604
1104	7	0.21528801737673575
1104	8	0.17526384620651214
1104	9	0.20568683916431496
1104	10-11	0.18565676202699422
1104	12-13	0.1884784270075457
1104	14-15	0.24971924876486185
1104	16-17	0.259011294874405
1104	18-19	0.23361707763417883
1104	20-21	0.2751232616599424
1104	22-23	0.2755672168630028
1104	24-25	0.3090768502421497
1104	26-27	0.3012329596159731
1104	28-29	0.31040633889454483
1104	30-31	0.3481980466082035
1104	32-33	0.3842520328463621
1104	34-35	0.329664740319906
1104	36-37	0.3203876637494929
1104	38-39	0.34356084197775516
1104	40-41	0.32325690885074465
1104	42-43	0.3646903558289054
1104	44-45	0.3507149721028213
1104	46-47	0.3889367163913988
1104	48-49	0.4496825501951278
1104	50-51	0.4113033531410366
1104	52-53	0.3944521731913113
1104	54-55	0.38317010446168354
1104	56-57	0.4005280833291849
1104	58-59	0.45209842070394757
1104	60-61	0.37315857636315286
1104	62-63	0.4189102310398951
1104	64-65	0.34777125533466346
1104	66-67	0.41637408742461446
1104	68-69	0.405485614778506
1104	70-71	0.40046339343241044
1104	72-73	0.41717791414221495
1104	74-75	0.38525110305290866
1104	76-77	0.34650325302060025
1104	78-79	0.3375138703723053
1104	80-81	0.34521779874279446
1104	82-83	0.3703880084858966
1104	84-85	0.4156472268533733
1104	86-87	0.3610308843068637
1104	88-89	0.37054154600094336
1104	90-91	0.3102867046287656
1104	92-93	0.43486997650389725
1104	94-95	0.4493462488050284
1104	96-97	0.48799619077682266
1104	98-99	0.4903310554669993
1104	100	0.4380127499180766
1105	1	0.1575391243849502
1105	2	0.15415223573832293
1105	3	0.3129496152793294
1105	4	0.1830196021980086
1105	5	0.17495727725630417
1105	6	0.1615257733620865
1105	7	0.1540898930146426
1105	8	0.17495383166733802
1105	9	0.20574685918084867
1105	10-11	0.21666256478562929
1105	12-13	0.1850100234002099
1105	14-15	0.2753065419227383
1105	16-17	0.2783958524339383
1105	18-19	0.24268792278758156
1105	20-21	0.22308166739039592
1105	22-23	0.2527913706391658
1105	24-25	0.24053037107857733
1105	26-27	0.27217498703234355
1105	28-29	0.2930822945657212
1105	30-31	0.2546212001028323
1105	32-33	0.3057167199896398
1105	34-35	0.3489614572971149
1105	36-37	0.3155923716261597
1105	38-39	0.324787630836191
1105	40-41	0.31521987487067094
1105	42-43	0.32457726853377267
1105	44-45	0.33750754530663585
1105	46-47	0.3868913940608891
1105	48-49	0.4087244958345124
1105	50-51	0.4226014095913513
1105	52-53	0.43109398769548335
1105	54-55	0.42732985979452565
1105	56-57	0.4337073940787164
1105	58-59	0.45012570674533947
1105	60-61	0.4162717969701717
1105	62-63	0.4668933087451208
1105	64-65	0.4693229651176125
1105	66-67	0.4914817510989451
1105	68-69	0.4817641757645461
1105	70-71	0.460134259614982
1105	72-73	0.5303689317618066
1105	74-75	0.48802839994199587
1105	76-77	0.4870136907012679
1105	78-79	0.4976871689128046
1105	80-81	0.5331043766394714
1105	82-83	0.5267604499111158
1105	84-85	0.4955568448653551
1105	86-87	0.4195275258260338
1105	88-89	0.4646961349255321
1105	90-91	0.4671264211483219
1105	92-93	0.5398264746854124
1105	94-95	0.4956895092057394
1105	96-97	0.538070922119271
1105	98-99	0.5465303146212541
1105	100	0.5520799613856298
1106	1	0.24995849271213189
1106	2	0.21975241830802617
1106	3	0.32581516635868724
1106	4	0.2136702865376563
1106	5	0.15501449811040402
1106	6	0.17275147096066945
1106	7	0.2240732353553767
1106	8	0.20100766551260563
1106	9	0.1818273891494968
1106	10-11	0.20211049825829264
1106	12-13	0.2167054010350853
1106	14-15	0.2902974026770053
1106	16-17	0.30222468325155916
1106	18-19	0.2656120679502294
1106	20-21	0.28008584170716233
1106	22-23	0.2429411847923646
1106	24-25	0.25039055669473953
1106	26-27	0.33383124969984124
1106	28-29	0.29506289018719656
1106	30-31	0.32626917796143573
1106	32-33	0.22699712263079164
1106	34-35	0.2945886353311167
1106	36-37	0.3071446534311306
1106	38-39	0.3327278172592827
1106	40-41	0.2934774633206345
1106	42-43	0.38148431465602783
1106	44-45	0.3081495920194328
1106	46-47	0.39654267145208166
1106	48-49	0.34265676970097303
1106	50-51	0.32126872511261695
1106	52-53	0.3136836414428146
1106	54-55	0.3523482523050845
1106	56-57	0.3719172431950213
1106	58-59	0.3707016147662472
1106	60-61	0.31906170218562124
1106	62-63	0.3452902874582833
1106	64-65	0.2800072773772797
1106	66-67	0.3174359068085266
1106	68-69	0.3394474257226321
1106	70-71	0.36314822487982923
1106	72-73	0.38572596363547973
1106	74-75	0.3591476660823929
1106	76-77	0.30199178435871943
1106	78-79	0.35833355704720304
1106	80-81	0.34854227490881584
1106	82-83	0.3574890607142507
1106	84-85	0.35145402732382536
1106	86-87	0.34844173065274475
1106	88-89	0.32890562565879833
1106	90-91	0.32767731148538815
1106	92-93	0.40955265339246694
1106	94-95	0.4124488204746726
1106	96-97	0.40187448743268916
1106	98-99	0.4322520048231411
1106	100	0.40701728554865113
1107	1	0.15186177479016294
1107	2	0.08208851447738041
1107	3	0.19031009166599233
1107	4	0.17250276202695858
1107	5	0.13680937471166033
1107	6	0.11466064484991279
1107	7	0.11569702655708625
1107	8	0.15308711056993474
1107	9	0.14966974439893477
1107	10-11	0.15123244070270658
1107	12-13	0.16417029947218964
1107	14-15	0.22668488641922835
1107	16-17	0.13556416028064433
1107	18-19	0.18778494697421166
1107	20-21	0.14901258924411565
1107	22-23	0.17022763404221308
1107	24-25	0.15084410439939688
1107	26-27	0.25173527133658524
1107	28-29	0.24338950645966406
1107	30-31	0.2061185727564947
1107	32-33	0.23902131501234436
1107	34-35	0.19696690668173744
1107	36-37	0.250584542019503
1107	38-39	0.2795157271466451
1107	40-41	0.30119389289085774
1107	42-43	0.29055920740913876
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2216	84-85	-1.264779796787444
2216	86-87	-1.0933922097345317
2216	88-89	-1.4050951654653616
2216	90-91	-1.2447314965570655
2216	92-93	-1.3166415263540188
2216	94-95	-1.3216544684138256
2216	96-97	-1.3239875986779914
2216	98-99	-1.468242345550948
2216	100	-1.4084044224020715
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	314.0
3	38.0
4	47.0
5	130.0
6	323.0
7	938.0
8	2139.0
9	3795.0
10	5939.0
11	8501.0
12	11927.0
13	16911.0
14	20200.0
15	25735.0
16	31110.0
17	42577.0
18	41688.0
19	23540.0
20	18733.0
21	18738.0
22	20054.0
23	22514.0
24	26426.0
25	33211.0
26	42574.0
27	54867.0
28	71424.0
29	94765.0
30	123691.0
31	159974.0
32	205866.0
33	269647.0
34	366695.0
35	530077.0
36	863695.0
37	1551855.0
38	2151019.0
39	854997.0
40	2702.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.247210240139005	20.726759171833656	14.711854188896838	23.3141763991305
2	15.697538246614753	20.594605056159978	39.651689462982496	24.056167234242768
3	17.80563610322907	25.402299356839205	30.928523238147747	25.863541301783975
4	12.794045940524695	17.353989417792018	38.08989902287388	31.762065618809405
5	12.707322457151976	37.665738785103876	35.692431616234266	13.934507141509883
6	30.625610152945004	38.200509989408474	17.212233216778145	13.96164664086838
7	26.36218264274211	33.02868003838652	22.50430604753545	18.10483127133592
8	24.80035857987859	36.39496381815025	20.279764850362856	18.524912751608298
9	24.607507135291765	16.63819718070476	21.700730732639528	37.053564951363946
10-11	23.76903262647136	27.037386182510087	28.72657064508841	20.467010545930137
12-13	24.3627403491776	24.53169474419185	29.08484494045642	22.02071996617413
14-15	21.705783205855298	26.534914965199874	26.690024793477292	25.069277035467536
16-17	20.803977005910454	29.87493806169541	27.433853129301273	21.887231803092867
18-19	20.650669721147562	28.251685581315932	30.26589801148915	20.83174668604735
20-21	21.685307329827907	27.43909978658231	30.117116430299134	20.758476453290648
22-23	21.3756526434261	27.276997518970447	30.189901359902667	21.15744847770079
24-25	21.24470277298572	27.41010307966882	30.11712485826238	21.228069289083084
26-27	21.025201272849657	27.409457660545446	30.169630379258834	21.39571068734607
28-29	21.087611003517136	27.35842396056663	30.321277050528867	21.232687985387372
30-31	21.38076592988422	27.70701643591612	29.906177637438265	21.006039996761398
32-33	21.071293128156633	27.5224476114446	30.206963334083607	21.19929592631516
34-35	21.255817264397756	27.25946017755461	30.19527561278791	21.289446945259726
36-37	21.531036760057056	27.513998712718866	29.689577666987223	21.265386860236855
38-39	21.383193822486653	27.647605653946083	29.767394983413116	21.201805540154147
40-41	21.296830846632517	27.440413002909757	29.87585959958232	21.3868965508754
42-43	21.21505893478553	27.5100551051175	29.721805789680783	21.553080170416177
44-45	21.399439189004937	27.634868269136163	29.497497621725334	21.468194920133563
46-47	21.51186978572146	27.8085117973057	29.117414202988236	21.562204213984604
48-49	21.53544712192727	27.732985374997654	28.986224813989324	21.745342689085753
50-51	21.569843647295325	27.757236303301497	28.793393271084504	21.87952677831867
52-53	21.50242543671042	27.87227754113164	28.774486495365565	21.850810526792376
54-55	21.57492967502474	28.126764439686987	28.619539633011442	21.67876625227683
56-57	21.485910712573872	27.867486080416203	28.645603121510387	22.001000085499538
58-59	21.361322673133518	27.548118417733058	28.947643702673826	22.142915206459595
60-61	21.518303134158383	27.865959371507564	28.686370542846745	21.929366951487307
62-63	21.37389870338152	27.91502737939013	28.667957369246732	22.04311654798162
64-65	21.373992401426854	27.677436061924432	28.798373281460186	22.150198255188524
66-67	21.3607670368574	27.717830352738293	28.62842234700096	22.29298026340335
68-69	21.653197434287577	27.86519872793498	28.456473059365344	22.025130778412098
70-71	21.626197348842766	27.699853070454417	28.49221941858805	22.18173016211477
72-73	21.4190049659273	27.876290911479256	28.497532193157067	22.20717192943638
74-75	21.531569630677456	27.88296025700226	28.3495628721714	22.235907240148876
76-77	21.619772102997924	27.866604320937316	28.211304349910073	22.302319226154683
78-79	21.64262803229351	27.93019659647164	28.27272195536379	22.15445341587106
80-81	21.559463486637302	27.80734700370791	28.26804396428944	22.365145545365344
82-83	21.469343661935156	27.832083745154097	28.37923994560889	22.319332647301856
84-85	21.536693410298543	27.742415186836748	28.221359880095736	22.499531522768965
86-87	21.60612669610513	27.855839670777012	28.16248150002335	22.375552133094512
88-89	21.46611264764184	27.683889953006318	28.229404243001195	22.620593156350644
90-91	21.34937194927195	27.624477088606064	28.24330725558728	22.782843706534706
92-93	21.453802629915288	27.75645715920232	28.083752116804057	22.70598809407834
94-95	21.35507094485441	27.564297053514313	28.245044042733515	22.83558795889777
96-97	21.395678673001314	27.58065396847975	28.1817900934088	22.841877265110135
98-99	21.351300249943307	27.62534556101737	28.111286339086146	22.91206784995318
100	21.387437793624134	27.559191192425704	28.08750574050768	22.965865273442486
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	715.0
1	555.5
2	742.0
3	1127.5
4	767.5
5	475.5
6	1083.5
7	2852.0
8	4855.0
9	5690.5
10	4688.5
11	3340.5
12	3258.0
13	3679.5
14	4272.0
15	4984.0
16	5700.5
17	6547.0
18	7476.5
19	8643.5
20	10330.5
21	12831.0
22	16455.5
23	21977.0
24	29515.0
25	39060.0
26	52794.5
27	69547.5
28	88876.0
29	111602.5
30	134807.0
31	155115.5
32	172339.5
33	190146.0
34	208315.0
35	222800.5
36	236007.0
37	250952.5
38	263897.5
39	271237.5
40	276016.5
41	280206.5
42	281418.0
43	282345.0
44	280371.5
45	275806.5
46	271416.5
47	262046.5
48	250648.5
49	242136.0
50	235533.5
51	226523.0
52	210914.5
53	197021.0
54	186604.0
55	175727.0
56	163401.5
57	149187.5
58	136724.0
59	124599.5
60	109796.5
61	93712.5
62	79584.5
63	66519.0
64	54317.0
65	44023.5
66	34864.5
67	26501.0
68	19982.0
69	15098.5
70	11262.0
71	8220.0
72	5791.0
73	3965.5
74	2741.0
75	1836.5
76	1126.0
77	687.5
78	432.0
79	252.5
80	141.5
81	67.5
82	36.0
83	21.0
84	11.0
85	9.5
86	8.0
87	3.5
88	3.5
89	2.0
90	1.5
91	1.0
92	0.0
93	0.0
94	0.5
95	1.0
96	0.5
97	0.0
98	0.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.035780094142324456
2	0.0
3	0.0
4	5.1817659873026E-5
5	0.0
6	0.0
7	0.0
8	4.663589388572341E-4
9	0.0
10-11	0.0
12-13	0.04389603511993716
14-15	9.068090477779551E-5
16-17	1.29544149682565E-5
18-19	8.420369729366726E-5
20-21	8.420369729366726E-5
22-23	0.0
24-25	9.068090477779551E-5
26-27	5.1817659873026E-5
28-29	3.3681478917466903E-4
30-31	1.29544149682565E-5
32-33	1.7488460207146277E-4
34-35	5.829486735715426E-5
36-37	7.124928232541077E-5
38-39	3.10905959238156E-4
40-41	1.4897577213494976E-4
42-43	4.987449762778753E-4
44-45	2.526110918810018E-4
46-47	9.715811226192376E-5
48-49	7.902193130636466E-4
50-51	4.404501089207211E-4
52-53	5.764714660874144E-4
54-55	4.469273164048493E-4
56-57	4.663589388572341E-4
58-59	1.1011252723018027E-4
60-61	1.9431622452384753E-4
62-63	8.549913879049291E-4
64-65	4.14541278984208E-4
66-67	0.0014508944764447282
68-69	0.0015091893438018823
70-71	3.8863244904769505E-4
72-73	9.391950851985963E-4
74-75	8.614685953890573E-4
76-77	7.189700307382358E-4
78-79	0.003102582384897432
80-81	0.0026167918235878132
82-83	0.001288964289341522
84-85	0.0028175852555957894
86-87	0.001418508439024087
88-89	0.0018978217928495775
90-91	0.0027787220106910196
92-93	0.002487247673905248
94-95	0.0036531450210483332
96-97	0.003141445629802202
98-99	0.002921220575341841
100	0.0014379400614764717
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7719376.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.123761065707946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.48866363804726	39.95847774356188
2	15.770408006985386	17.386484057715794
3	5.240808395073325	8.666792092835346
4	2.2301412313856512	4.917350895267454
5	1.168759141387948	3.22131998266156
6	0.7057284486137495	2.3341443827194244
7	0.48471272817278716	1.8703432029312912
8	0.31389955438244943	1.384265922752827
9	0.24111021830953056	1.1961811858135716
>10	1.2493414254529749	12.525563608889021
>50	0.07740390887415098	2.909373276775233
>100	0.028035350163329465	2.565403462220116
>500	5.411038511591532E-4	0.1895007502348812
>1k	3.762941546355699E-4	0.3417627049075702
>5k	2.3518381861831764E-5	0.11639273685060361
>10k+	4.703676372366353E-5	0.4166439938632991
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16111	0.2087085795535805	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16027	0.20762040869624696	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8978	0.11630473758500687	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.2954414968256502E-5	0.0
4	0.0	0.0	0.0	1.2954414968256502E-5	0.0
5	0.0	0.0	0.0	1.2954414968256502E-5	0.0
6	0.0	0.0	0.0	1.2954414968256502E-5	0.0
7	0.0	0.0	0.0	2.5908829936513004E-5	0.0
8	0.0	0.0	0.0	3.88632449047695E-5	0.0
9	0.0	0.0	0.0	6.477207484128251E-5	0.0
10-11	0.0	1.2954414968256502E-5	0.0	7.124928232541075E-5	0.0
12-13	0.0	3.238603742064126E-5	0.0	7.7726489809539E-5	0.0
14-15	0.0	7.7726489809539E-5	0.0	1.2306694219843677E-4	0.0
16-17	0.0	1.0363531974605202E-4	0.0	1.2954414968256503E-4	0.0
18-19	0.0	1.0363531974605202E-4	0.0	1.6840739458733452E-4	0.0
20-21	0.0	1.0363531974605202E-4	0.0	2.396566769127453E-4	0.0
22-23	0.0	1.0363531974605202E-4	0.0	4.534045238889776E-4	0.0
24-25	0.0	1.0363531974605202E-4	0.0	9.068090477779552E-4	0.0
26-27	0.0	1.2306694219843677E-4	0.0	0.0017164599832939866	0.0
28-29	0.0	1.2954414968256503E-4	0.0	0.005421422664215346	0.0
30-31	0.0	1.2954414968256503E-4	0.0	0.016996192438352528	0.0
32-33	0.0	1.2954414968256503E-4	0.0	0.04333251806881799	0.0
34-35	0.0	1.2954414968256503E-4	0.0	0.08698889651184241	0.0
36-37	0.0	1.2954414968256503E-4	0.0	0.15465628309853025	0.0
38-39	0.0	1.424985646508215E-4	0.0	0.24435912954622238	0.0
40-41	0.0	1.6840739458733452E-4	0.0	0.33707387747403417	0.0
42-43	0.0	1.6840739458733452E-4	0.0	0.42958783196983796	0.0
44-45	0.0	1.6840739458733452E-4	0.0	0.5429260085271141	0.0
46-47	0.0	1.7488460207146277E-4	0.0	0.6791611135407836	0.0
48-49	0.0	1.9431622452384753E-4	0.0	0.817715058833771	0.0
50-51	0.0	1.9431622452384753E-4	0.0	0.9553686722864646	0.0
52-53	0.0	2.0727063949210403E-4	0.0	1.0871409813435697	0.0
54-55	0.0	2.0727063949210403E-4	0.0	1.2343549530428366	0.0
56-57	0.0	2.0727063949210403E-4	0.0	1.407995931277347	0.0
58-59	0.0	2.0727063949210403E-4	0.0	1.5955434739802803	0.0
60-61	0.0	2.0727063949210403E-4	0.0	1.7845354339521744	0.0
62-63	0.0	2.0727063949210403E-4	0.0	1.9662016722595195	0.0
64-65	0.0	2.0727063949210403E-4	0.0	2.1599155164873434	0.0
66-67	0.0	2.0727063949210403E-4	0.0	2.3794539869543856	0.0
68-69	0.0	2.0727063949210403E-4	0.0	2.6197778162379963	0.0
70-71	0.0	2.4613388439687355E-4	0.0	2.8714497130337997	0.0
72-73	0.0	2.526110918810018E-4	0.0	3.1300522218376203	0.0
74-75	0.0	2.5908829936513005E-4	0.0	3.387670195103853	0.0
76-77	0.0	2.5908829936513005E-4	0.0	3.6646874047850497	0.0
78-79	0.0	2.5908829936513005E-4	0.0	3.971188862933999	0.0
80-81	0.0	2.84997129301643E-4	0.0	4.296370587467173	0.0
82-83	0.0	2.979515442698995E-4	0.0	4.6247455752900235	0.0
84-85	0.0	2.979515442698995E-4	0.0	4.9514429663744846	0.0
86-87	0.0	3.238603742064125E-4	0.0	5.296069267775012	0.0
88	0.0	3.3681478917466903E-4	0.0	5.567950051921295	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12505	0.0	55.9817	1
GTATCAA	22115	0.0	39.138298	1
TCAACGC	26865	0.0	31.83775	4
ATCAACG	27050	0.0	31.620005	3
CAACGCA	27700	0.0	30.962849	5
TATCAAC	28490	0.0	30.170261	2
GTGGTAT	5725	0.0	29.728168	1
AACGCAG	29105	0.0	29.574184	6
TGGTATC	6035	0.0	26.943665	2
ACGCAGA	33805	0.0	25.281525	7
CGCAGAG	34220	0.0	24.87871	8
GTACATG	20150	0.0	24.289064	1
TACATGG	20420	0.0	22.669312	2
GCAGAGT	37540	0.0	22.227398	9
ACATGGG	20960	0.0	21.165989	3
GAGTACT	26135	0.0	20.8859	12-13
CAGAGTA	36185	0.0	20.349503	10-11
TATAACG	510	0.0	20.272642	2
AGAGTAC	34555	0.0	19.696404	10-11
GTACTTT	28350	0.0	19.129929	14-15
>>END_MODULE