FastQCFastQC Report
Wed 25 May 2016
SRR2049365_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049365_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6036662
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62350.043.9615061
GTATCAA103500.034.2054331
TCAACGC109050.031.3407634
ATCAACG109800.031.1266883
CAACGCA112800.030.2579255
AACGCAG120900.028.4583726
TATCAAC124100.027.9187872
GTACATG149000.024.485891
ACGCAGA139650.024.4354847
CGCAGAG138650.024.2049188
TACATGG148650.023.339542
ACATGGG155700.021.3467453
GTGGTAT36400.021.1827771
GCAGAGT158050.020.4309029
TATAACG5650.019.9693372
CATGGGG110300.018.625454
CAGAGTA150500.018.02040910-11
AGAGTAC139000.017.90508710-11
GTCTAGG15250.017.5729831
TCTGTCG18350.017.1618468
Produced by FastQC (version 0.11.5)