##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049365_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6036662 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.900829465025538 31.0 31.0 34.0 28.0 34.0 2 30.985269176906044 31.0 31.0 34.0 28.0 34.0 3 31.164930221370685 31.0 31.0 34.0 28.0 34.0 4 34.748135973158675 37.0 35.0 37.0 32.0 37.0 5 34.80109454529672 37.0 35.0 37.0 32.0 37.0 6 34.75592123594132 37.0 35.0 37.0 32.0 37.0 7 34.729050591204214 37.0 35.0 37.0 32.0 37.0 8 34.71542567730312 37.0 35.0 37.0 32.0 37.0 9 36.3098096265784 39.0 37.0 39.0 32.0 39.0 10-11 36.271431131973266 39.0 37.0 39.0 32.0 39.0 12-13 36.2054501312149 39.0 37.0 39.0 32.0 39.0 14-15 37.41774237152917 40.0 38.0 41.0 32.0 41.0 16-17 37.380754214829324 40.0 38.0 41.0 32.0 41.0 18-19 37.4033957839614 40.0 38.0 41.0 32.0 41.0 20-21 37.491625918429754 40.0 38.0 41.0 32.0 41.0 22-23 37.437727340043224 40.0 38.0 41.0 32.0 41.0 24-25 37.446191206332244 40.0 38.0 41.0 32.0 41.0 26-27 37.44571229928063 40.0 38.0 41.0 32.0 41.0 28-29 37.38644162949657 40.0 38.0 41.0 32.0 41.0 30-31 37.319977745979486 40.0 38.0 41.0 32.0 41.0 32-33 37.269031875563016 40.0 37.5 41.0 31.0 41.0 34-35 37.16518996756817 40.0 37.0 41.0 31.0 41.0 36-37 37.073517367710835 40.0 37.0 41.0 31.0 41.0 38-39 36.96766847307336 40.0 37.0 41.0 30.0 41.0 40-41 36.86027004659198 40.0 37.0 41.0 30.0 41.0 42-43 36.71117456965455 40.0 37.0 41.0 30.0 41.0 44-45 36.5739747396823 40.0 36.5 41.0 30.0 41.0 46-47 36.453574674215645 40.0 36.0 41.0 30.0 41.0 48-49 36.32487730802222 40.0 36.0 41.0 30.0 41.0 50-51 36.188630074037604 40.0 36.0 41.0 29.5 41.0 52-53 36.051047747911014 39.0 35.0 41.0 29.0 41.0 54-55 35.91105490087071 39.0 35.0 41.0 29.0 41.0 56-57 35.78982515502773 39.0 35.0 41.0 28.5 41.0 58-59 35.59275175585448 39.0 35.0 41.0 28.0 41.0 60-61 35.377696316275454 39.0 35.0 40.0 28.0 41.0 62-63 35.16508999509994 38.0 34.5 40.0 28.0 41.0 64-65 34.91404753156628 38.0 34.0 40.0 28.0 41.0 66-67 34.65275644056268 37.5 34.0 40.0 27.0 41.0 68-69 34.362995228157544 37.0 34.0 40.0 26.0 41.0 70-71 34.07055057911144 36.5 34.0 39.5 26.0 41.0 72-73 33.724640123962544 36.0 34.0 39.0 26.0 41.0 74-75 33.37487952779202 35.5 33.0 39.0 26.0 40.5 76-77 33.03804105646465 35.0 33.0 38.0 25.5 40.0 78-79 32.71587576047823 35.0 33.0 37.0 25.0 39.0 80-81 32.353022249713504 35.0 33.0 37.0 24.5 39.0 82-83 32.031431443403655 35.0 33.0 36.0 24.0 39.0 84-85 31.725879136516173 35.0 33.0 36.0 23.5 37.0 86-87 31.396633023349658 35.0 32.0 36.0 21.5 37.0 88-89 31.133073791443017 35.0 32.0 35.0 20.0 37.0 90-91 30.913481987230693 35.0 32.0 35.0 19.5 36.0 92-93 30.659976573145887 35.0 32.0 35.0 17.5 36.0 94-95 30.44309313657117 35.0 32.0 35.0 9.0 36.0 96-97 30.179252292077972 35.0 32.0 35.0 2.0 35.5 98-99 29.957185693020413 35.0 32.0 35.0 2.0 35.0 100 29.83343526604604 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.40027599811590875 1101 2 0.3695340334422319 1101 3 0.3656306022529847 1101 4 0.4332590909993641 1101 5 0.4434684236510762 1101 6 0.49215836871299956 1101 7 0.4999121206367292 1101 8 0.533269626625362 1101 9 0.6028445869182875 1101 10-11 0.6470439598672897 1101 12-13 0.6303716612236556 1101 14-15 0.7505077829335036 1101 16-17 0.7668132561302201 1101 18-19 0.7018972867930486 1101 20-21 0.6806948778076816 1101 22-23 0.6861359778010083 1101 24-25 0.713041149899972 1101 26-27 0.6579329479237188 1101 28-29 0.7074135942463755 1101 30-31 0.6703997417027381 1101 32-33 0.6480236034001194 1101 34-35 0.6226626607003922 1101 36-37 0.673825295912124 1101 38-39 0.6711399957965654 1101 40-41 0.7358201520498682 1101 42-43 0.6739234095035087 1101 44-45 0.6251387283497323 1101 46-47 0.6377271674048188 1101 48-49 0.5904537711241886 1101 50-51 0.5790890143475664 1101 52-53 0.5891173935831588 1101 54-55 0.6287629345225412 1101 56-57 0.6288285450015607 1101 58-59 0.6760277898371143 1101 60-61 0.627880011319256 1101 62-63 0.6652003582985913 1101 64-65 0.6637714513424342 1101 66-67 0.6867831224992571 1101 68-69 0.6070260737337634 1101 70-71 0.6491028045504237 1101 72-73 0.7299952812354888 1101 74-75 0.6841517326248052 1101 76-77 0.6869812651396217 1101 78-79 0.6057489259529802 1101 80-81 0.677921988243682 1101 82-83 0.6468216639935704 1101 84-85 0.6022115456882879 1101 86-87 0.5670180052409677 1101 88-89 0.6273422451344466 1101 90-91 0.6676462610101126 1101 92-93 0.6561787647270911 1101 94-95 0.708745521143765 1101 96-97 0.648955828508953 1101 98-99 0.7152179551211049 1101 100 0.7132110502542233 1102 1 0.2655191134151629 1102 2 0.3002539175418839 1102 3 0.34529415346837666 1102 4 0.36743195596626776 1102 5 0.30742943769359243 1102 6 0.3882513426820964 1102 7 0.44038446585500424 1102 8 0.47183825307728 1102 9 0.5727008196083219 1102 10-11 0.5313464039008622 1102 12-13 0.6028565528111542 1102 14-15 0.6824695266978154 1102 16-17 0.6566715055318895 1102 18-19 0.5824338247262872 1102 20-21 0.580261348943587 1102 22-23 0.5818158964484255 1102 24-25 0.6439402576654132 1102 26-27 0.5623781865423894 1102 28-29 0.5694728634770243 1102 30-31 0.6020570617780265 1102 32-33 0.6305598569984028 1102 34-35 0.6189410082368241 1102 36-37 0.5935507594618343 1102 38-39 0.6201450051514783 1102 40-41 0.6974333645752822 1102 42-43 0.6834729024929445 1102 44-45 0.595091214481279 1102 46-47 0.5381455032329185 1102 48-49 0.5794635550260523 1102 50-51 0.5783785973906603 1102 52-53 0.5858286856361374 1102 54-55 0.5463380062565477 1102 56-57 0.5113968023168596 1102 58-59 0.5787536000759488 1102 60-61 0.6141267577683678 1102 62-63 0.5696916534799641 1102 64-65 0.5632554510873149 1102 66-67 0.5945668882557058 1102 68-69 0.6246273413403571 1102 70-71 0.6275573264561487 1102 72-73 0.5569782200689133 1102 74-75 0.5565891067807485 1102 76-77 0.6789090241062468 1102 78-79 0.5909091066588914 1102 80-81 0.5853294555638726 1102 82-83 0.5784799461044443 1102 84-85 0.5677374353128748 1102 86-87 0.5802099288453633 1102 88-89 0.5771099491723959 1102 90-91 0.6389346012457509 1102 92-93 0.6079594605211049 1102 94-95 0.5836342332239717 1102 96-97 0.5853546514049768 1102 98-99 0.6320440643392224 1102 100 0.6033155593862567 1103 1 0.1592808135922006 1103 2 0.20808417448477812 1103 3 0.20730329174239515 1103 4 0.22754361304038895 1103 5 0.1895307216152844 1103 6 0.2730797493164516 1103 7 0.2886191332610082 1103 8 0.28038078595614735 1103 9 0.3471068676346789 1103 10-11 0.3642726337891631 1103 12-13 0.39419981186934194 1103 14-15 0.4307294867942275 1103 16-17 0.43087837633630954 1103 18-19 0.4105229565365036 1103 20-21 0.3758393281868564 1103 22-23 0.3692337047070353 1103 24-25 0.3920589916430046 1103 26-27 0.3514744998368329 1103 28-29 0.3852721260685854 1103 30-31 0.3996221299119114 1103 32-33 0.4374635573874315 1103 34-35 0.4279521387038798 1103 36-37 0.34663293279571405 1103 38-39 0.4298210393444961 1103 40-41 0.4471416678538631 1103 42-43 0.3802891158568187 1103 44-45 0.2900581713568684 1103 46-47 0.32709572381905616 1103 48-49 0.36076106026975197 1103 50-51 0.3067586548699026 1103 52-53 0.3632771054891535 1103 54-55 0.3343824506937523 1103 56-57 0.3186744970526618 1103 58-59 0.3451054425219269 1103 60-61 0.3422702596320306 1103 62-63 0.3919603578534776 1103 64-65 0.3659961540959671 1103 66-67 0.41547482769529864 1103 68-69 0.42355185085872193 1103 70-71 0.3453925162338649 1103 72-73 0.40207886494020784 1103 74-75 0.3696243433863202 1103 76-77 0.48058880382200186 1103 78-79 0.33751807797292344 1103 80-81 0.38453104167293617 1103 82-83 0.36068077723376746 1103 84-85 0.3791010289773844 1103 86-87 0.39928853291257127 1103 88-89 0.3392111006033929 1103 90-91 0.4118978174855492 1103 92-93 0.41366731023626 1103 94-95 0.4311166494284926 1103 96-97 0.4226284289774256 1103 98-99 0.37693832644968595 1103 100 0.3620979547180667 1104 1 0.1765530539800686 1104 2 0.14868486227370425 1104 3 0.2011653393238575 1104 4 0.22951677799697734 1104 5 0.25078809749381037 1104 6 0.26694649490971756 1104 7 0.2929295590887975 1104 8 0.3231232973253313 1104 9 0.3792624094093995 1104 10-11 0.44093126685564954 1104 12-13 0.4069455178865553 1104 14-15 0.49734494513226224 1104 16-17 0.516759206568949 1104 18-19 0.5045654077711319 1104 20-21 0.45712197936887833 1104 22-23 0.4956278397090941 1104 24-25 0.517861177158089 1104 26-27 0.481307579477523 1104 28-29 0.5350987694331053 1104 30-31 0.4723262115445621 1104 32-33 0.4890629955052148 1104 34-35 0.5048762094371497 1104 36-37 0.43128640744524915 1104 38-39 0.5183879578245936 1104 40-41 0.5387998780992902 1104 42-43 0.4863302892322494 1104 44-45 0.44200244354087914 1104 46-47 0.31632200825548296 1104 48-49 0.34069115385415216 1104 50-51 0.3941393253701406 1104 52-53 0.39826554838011674 1104 54-55 0.3340841269443615 1104 56-57 0.3004203721982961 1104 58-59 0.3933528492194185 1104 60-61 0.2966924981406933 1104 62-63 0.33668824900994565 1104 64-65 0.3734401537952934 1104 66-67 0.4013349866760194 1104 68-69 0.3031864395008057 1104 70-71 0.32871809784772665 1104 72-73 0.402580377700005 1104 74-75 0.42326036962001723 1104 76-77 0.45219284599135534 1104 78-79 0.39758215885388637 1104 80-81 0.4407803369711729 1104 82-83 0.3615452114052218 1104 84-85 0.4154331032435543 1104 86-87 0.4576319669712774 1104 88-89 0.4064537435169093 1104 90-91 0.3933359728581962 1104 92-93 0.4225218879789594 1104 94-95 0.45170333714225563 1104 96-97 0.4338919122712994 1104 98-99 0.4457900384051854 1104 100 0.3722362641855028 1105 1 0.17082172703766219 1105 2 0.2037706845780285 1105 3 0.21936928715597048 1105 4 0.25817550880103113 1105 5 0.2867490888719715 1105 6 0.30669040836068007 1105 7 0.31505983662273707 1105 8 0.3916318815645212 1105 9 0.43687070378526016 1105 10-11 0.4406101280907748 1105 12-13 0.4359823312886917 1105 14-15 0.5085113513449357 1105 16-17 0.4449444301857284 1105 18-19 0.39701117073509096 1105 20-21 0.3840560980613148 1105 22-23 0.4152827985082226 1105 24-25 0.4051268853808594 1105 26-27 0.41328017936264416 1105 28-29 0.4436332177010769 1105 30-31 0.4121553573372836 1105 32-33 0.4679207633471236 1105 34-35 0.49207013524195276 1105 36-37 0.38578797903390694 1105 38-39 0.3576132893761397 1105 40-41 0.3931499546372166 1105 42-43 0.3657303248134127 1105 44-45 0.404507680495783 1105 46-47 0.41665132177923425 1105 48-49 0.36023315076084117 1105 50-51 0.4511883561939598 1105 52-53 0.38583419430205623 1105 54-55 0.4534155626739462 1105 56-57 0.4620212502512473 1105 58-59 0.4823581129431602 1105 60-61 0.47048961861681704 1105 62-63 0.392639757318463 1105 64-65 0.4352145183977427 1105 66-67 0.45513420149741535 1105 68-69 0.4061334226086615 1105 70-71 0.4110752127133068 1105 72-73 0.3768946914145914 1105 74-75 0.5019542143322653 1105 76-77 0.541325316299563 1105 78-79 0.44767002441124504 1105 80-81 0.468763252773158 1105 82-83 0.49400508281522093 1105 84-85 0.4433552760455619 1105 86-87 0.4839477472515732 1105 88-89 0.44519306119878266 1105 90-91 0.48037802806244656 1105 92-93 0.45626163317626833 1105 94-95 0.44150768354626635 1105 96-97 0.44287707272583177 1105 98-99 0.44283647007490146 1105 100 0.4603288108403625 1106 1 0.15156541619935737 1106 2 0.12376337741211074 1106 3 0.1696095002908855 1106 4 0.16847995074305544 1106 5 0.15998745556730398 1106 6 0.18962619908113254 1106 7 0.17354236897641329 1106 8 0.2533733592899594 1106 9 0.3258832431327008 1106 10-11 0.28021596784469693 1106 12-13 0.25384364484759203 1106 14-15 0.3631617199698809 1106 16-17 0.3386939043277408 1106 18-19 0.2980431180695007 1106 20-21 0.2701376767466641 1106 22-23 0.3493332936524922 1106 24-25 0.3422578234777589 1106 26-27 0.24427314630585073 1106 28-29 0.3154772621438866 1106 30-31 0.3396272756165999 1106 32-33 0.3588754815800641 1106 34-35 0.35593988229732787 1106 36-37 0.26090037703554003 1106 38-39 0.25475724464306637 1106 40-41 0.2545379412803612 1106 42-43 0.27040018483935313 1106 44-45 0.31399720829761435 1106 46-47 0.27014744266497104 1106 48-49 0.2967313415682753 1106 50-51 0.3392673213199089 1106 52-53 0.35721946757824696 1106 54-55 0.38931375102235677 1106 56-57 0.2887230351910617 1106 58-59 0.34030301049939027 1106 60-61 0.374435277265853 1106 62-63 0.31745266036072906 1106 64-65 0.32919819673007567 1106 66-67 0.35600259343633667 1106 68-69 0.35532146171104273 1106 70-71 0.3524486766354116 1106 72-73 0.3988923647415987 1106 74-75 0.43048531607496443 1106 76-77 0.4257063275042796 1106 78-79 0.30785053262452067 1106 80-81 0.39086928599461146 1106 82-83 0.32861641680573683 1106 84-85 0.33812993519917356 1106 86-87 0.3760533441163645 1106 88-89 0.34396711596226837 1106 90-91 0.3783676835166787 1106 92-93 0.403802227002231 1106 94-95 0.4611597409437991 1106 96-97 0.4080683415907238 1106 98-99 0.4085279880970347 1106 100 0.41090451233190706 1107 1 0.1077470434162322 1107 2 0.12237256401457941 1107 3 0.1416619439423279 1107 4 0.15831822874545054 1107 5 0.1552165722758332 1107 6 0.21027622955691072 1107 7 0.2178671248010815 1107 8 0.2852411133831012 1107 9 0.3235272227423991 1107 10-11 0.2996324146428435 1107 12-13 0.30309329738150836 1107 14-15 0.28838469934125754 1107 16-17 0.4059670812116991 1107 18-19 0.33641455140135434 1107 20-21 0.3089886554515786 1107 22-23 0.3257052310474293 1107 24-25 0.3764396278548858 1107 26-27 0.4057783335157339 1107 28-29 0.37561583731149994 1107 30-31 0.30620567851696734 1107 32-33 0.3139638297083849 1107 34-35 0.3495215668803766 1107 36-37 0.3739673161915391 1107 38-39 0.37209137308846607 1107 40-41 0.434740441770721 1107 42-43 0.3789935113815517 1107 44-45 0.2852637142546186 1107 46-47 0.3459675997048848 1107 48-49 0.3064041329177911 1107 50-51 0.35835095993830635 1107 52-53 0.3907999816382315 1107 54-55 0.3785175738874216 1107 56-57 0.35998880298037506 1107 58-59 0.38652253735499187 1107 60-61 0.3274466323941141 1107 62-63 0.2845613840266026 1107 64-65 0.252070428337575 1107 66-67 0.2966855989177688 1107 68-69 0.3384128869441412 1107 70-71 0.35535995731418524 1107 72-73 0.38956029051145435 1107 74-75 0.42345685232849206 1107 76-77 0.469306519006075 1107 78-79 0.3527287560446766 1107 80-81 0.3494742123149308 1107 82-83 0.36084033681366634 1107 84-85 0.36425964813630074 1107 86-87 0.43216748171504094 1107 88-89 0.4574298184574843 1107 90-91 0.45484096079144365 1107 92-93 0.4532152255026958 1107 94-95 0.44377251715033594 1107 96-97 0.36100429816115565 1107 98-99 0.3460062385130449 1107 100 0.3620048040135977 1108 1 0.08100368555089332 1108 2 0.11908068021358531 1108 3 0.17436996309882957 1108 4 0.17469623424625524 1108 5 0.21692857236164542 1108 6 0.26774201016291954 1108 7 0.2465033178660434 1108 8 0.2805302476771985 1108 9 0.30511376454924743 1108 10-11 0.26676118868643783 1108 12-13 0.2615557857469426 1108 14-15 0.33288730713985615 1108 16-17 0.3581563448904248 1108 18-19 0.3283405622011131 1108 20-21 0.3068559703603242 1108 22-23 0.36432372214193265 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-0.5909573222099667 2215 38-39 -0.42739503503787546 2215 40-41 -0.5478340523931919 2215 42-43 -0.4255074317609058 2215 44-45 -0.363960619173632 2215 46-47 -0.4347792522818139 2215 48-49 -0.3666939772084845 2215 50-51 -0.2796726994305274 2215 52-53 -0.3320805316504405 2215 54-55 -0.4055301454556357 2215 56-57 -0.38812421483495285 2215 58-59 -0.3412500259864615 2215 60-61 -0.17737443895523342 2215 62-63 -0.248912672704094 2215 64-65 -0.23340921134440862 2215 66-67 -0.2466792704084 2215 68-69 -0.2822917989686857 2215 70-71 -0.2538057362706283 2215 72-73 -0.272663895628142 2215 74-75 -0.23653567033552747 2215 76-77 -0.23263488052764103 2215 78-79 -0.32918420610538845 2215 80-81 -0.25964209515977643 2215 82-83 -0.2786247568089024 2215 84-85 -0.2318487171235546 2215 86-87 -0.17357019213299552 2215 88-89 -0.15119341010720788 2215 90-91 -0.20393786875558462 2215 92-93 -0.13415222023770568 2215 94-95 -0.12019255587282274 2215 96-97 -0.09066335247113955 2215 98-99 -0.2928962743965329 2215 100 -0.2375982421970093 2216 1 -1.1913101932637744 2216 2 -1.1174974364380006 2216 3 -1.2500885766156635 2216 4 -1.4402568561449343 2216 5 -1.2991878901646032 2216 6 -2.720152997030212 2216 7 -2.5839663090406972 2216 8 -3.4851801375227716 2216 9 -2.898816367192417 2216 10-11 -2.057959268252496 2216 12-13 -1.8730882843648615 2216 14-15 -2.139335834211778 2216 16-17 -2.543846469231056 2216 18-19 -2.1378648701084018 2216 20-21 -1.9581791086629892 2216 22-23 -2.0799582239958667 2216 24-25 -2.2584101726407866 2216 26-27 -2.3300728763543574 2216 28-29 -2.7381099992250597 2216 30-31 -2.3554564251397494 2216 32-33 -2.5130944917156057 2216 34-35 -3.552116261784903 2216 36-37 -2.497353754128241 2216 38-39 -2.415787697517807 2216 40-41 -2.9292230460357658 2216 42-43 -2.804239315058659 2216 44-45 -2.5215870744869946 2216 46-47 -2.3240516443273336 2216 48-49 -2.468302328655966 2216 50-51 -2.7898607871543106 2216 52-53 -3.1448675538730484 2216 54-55 -2.5063505787269875 2216 56-57 -2.4974128799873156 2216 58-59 -3.561192434209808 2216 60-61 -4.322035894779521 2216 62-63 -3.391023465370786 2216 64-65 -3.4159828336559244 2216 66-67 -3.1253945080986085 2216 68-69 -3.504124459552788 2216 70-71 -3.3510032917269044 2216 72-73 -3.7426096297506675 2216 74-75 -4.181227218987701 2216 76-77 -4.904322892347274 2216 78-79 -3.857817813570456 2216 80-81 -3.6694314506122936 2216 82-83 -3.941567034767477 2216 84-85 -3.874234737729875 2216 86-87 -3.573033627234178 2216 88-89 -3.4992068235944167 2216 90-91 -3.7041947846855905 2216 92-93 -3.7465062228153627 2216 94-95 -3.567956471220956 2216 96-97 -3.859902951933261 2216 98-99 -3.684914745165486 2216 100 -3.311391949150149 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 91445.0 3 15801.0 4 10343.0 5 8575.0 6 7696.0 7 7947.0 8 8976.0 9 10280.0 10 11741.0 11 13441.0 12 15016.0 13 16756.0 14 19171.0 15 20112.0 16 19964.0 17 21206.0 18 19702.0 19 18013.0 20 18354.0 21 19130.0 22 21233.0 23 25260.0 24 30312.0 25 37596.0 26 45468.0 27 54007.0 28 66017.0 29 83585.0 30 101666.0 31 121650.0 32 156299.0 33 206797.0 34 285414.0 35 412932.0 36 673381.0 37 1194017.0 38 1568561.0 39 577085.0 40 1713.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.78037732747881 20.31909152710644 13.640944904329372 23.259586241085376 2 15.738369135926664 20.458547419964347 40.465269365908036 23.337814078200957 3 18.108730675522462 25.441296893880423 30.70394538291638 25.74602704768073 4 12.561840307898025 17.18327735318013 39.04628116212876 31.208601176793096 5 12.624505755905075 37.5442698181606 35.63610971887422 14.1951147070601 6 30.19395810910566 38.20365502014196 18.042191208872076 13.560195661880305 7 26.216811959295967 32.62696807981703 23.20945341244324 17.946766548443765 8 24.17358224269342 36.544570350935544 21.190607710542018 18.09123969582901 9 24.338341630407363 17.309169230810024 22.2489639291902 36.103525209592405 10-11 23.29344485137281 27.155897207441654 29.41867526088771 20.131982680297828 12-13 23.841630124291726 24.87056760817923 29.268778769947073 22.019023497581976 14-15 21.422088959365286 27.23867622959514 26.476079309814487 24.863155501225087 16-17 20.880365457231708 30.37420752276443 26.99423359133986 21.751193428664 18-19 20.707652897058612 29.244314357312085 29.14724619854358 20.900786547085723 20-21 21.95170255199669 28.71981533796646 28.487927129561402 20.84055498047545 22-23 21.628573602019138 28.733549303323592 28.458302749001756 21.17957434565551 24-25 21.39992814141822 28.69769247112316 28.531734943920146 21.370644443538477 26-27 21.128671082052325 28.483803255258657 28.749023044547844 21.638502618141175 28-29 21.27872674223635 28.576738925960555 28.74345990952818 21.401074422274917 30-31 21.596694744927827 29.105328074822328 28.20437096118637 21.09360621906347 32-33 21.0567123712203 28.762380790995095 28.76792433599683 21.412982501787774 34-35 21.20525134420809 28.312003773262205 28.858081058846846 21.624663823682855 36-37 21.688455220635415 28.85613157517938 28.12435368485806 21.33105951932714 38-39 21.528402056649078 28.899696388136626 28.330431394664846 21.24147016054945 40-41 21.246500730181523 28.584808445843603 28.614340526433367 21.554350297541514 42-43 21.14529542004468 28.62857384341047 28.528534401039423 21.697596335505427 44-45 21.413343904215086 28.732400691659155 28.447159235727902 21.407096168397857 46-47 21.30447643953028 29.055633996546387 28.18103628282507 21.45885328109826 48-49 21.218909982546304 28.6997626217798 28.450402800545948 21.630924595127947 50-51 21.26858551122901 28.487466253641035 28.46395017574498 21.779998059384976 52-53 21.20223929491759 28.527071377214764 28.45670499557393 21.81398433229372 54-55 21.365299912266718 29.02414703660702 28.21223300925823 21.398320041868033 56-57 21.16290383001374 28.750097670823266 28.22457685436396 21.862421644799035 58-59 21.001664997581305 28.311090554357854 28.61917393780794 22.068070510252902 60-61 21.410875511856712 28.74977741757656 28.134376893089925 21.704970177476802 62-63 21.288962711767784 28.762253153367695 28.119548319322508 21.829235815542013 64-65 21.24969736279328 28.442130546049494 28.385331694521444 21.922840396635785 66-67 21.166980478901444 28.517710311921956 28.140704766708968 22.174604442467626 68-69 21.64756894527382 28.6935385375142 27.974625123149856 21.684267394062125 70-71 21.64267468402804 28.4872257275898 28.110276724252508 21.759822864129656 72-73 21.356736682329274 28.600454839562044 28.381353980832174 21.661454497276505 74-75 21.44005793441281 28.574160522735447 28.226852335191012 21.75892920766073 76-77 21.513708620838802 28.70170711877844 27.95468729337141 21.829896967011347 78-79 21.62253351767787 28.81284041553419 28.01832275970597 21.546303307081967 80-81 21.490279587444356 28.572308061986924 28.15798751727095 21.77942483329777 82-83 21.375393222919094 28.66286227341884 28.229962844856686 21.731781658805385 84-85 21.53491861421271 28.549009287833343 28.08532192220996 21.830750175743983 86-87 21.706535833280235 28.658329993421816 27.980244406121198 21.654889767176748 88-89 21.544549053973526 28.54298399878561 28.06859528839506 21.84387165884581 90-91 21.495578781298928 28.418118068476584 28.059291519537837 22.02701163068665 92-93 21.68671833680691 28.62804119436856 27.855549424931542 21.829691043892993 94-95 21.610599484430114 28.404261410221427 28.029006212574842 21.95613289277361 96-97 21.66841542561104 28.375565834230905 28.021305483063326 21.93471325709473 98-99 21.67508301773397 28.427771838144988 27.9445412050567 21.95260393906434 100 21.831336589658324 28.40770279999112 27.80319653477369 21.957764075576865 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3700.0 1 2770.0 2 2256.5 3 2675.5 4 2502.5 5 2107.5 6 1740.5 7 1566.0 8 1586.5 9 1644.0 10 1450.5 11 1229.5 12 1251.5 13 1341.0 14 1519.5 15 1743.0 16 2046.0 17 2601.5 18 3378.0 19 4536.0 20 6521.5 21 9453.5 22 13248.5 23 18752.0 24 26490.0 25 36239.5 26 48860.5 27 63386.0 28 79869.5 29 99252.5 30 118536.0 31 135275.0 32 148559.5 33 160074.0 34 172474.0 35 183315.0 36 191664.0 37 200886.5 38 209468.0 39 212640.0 40 214512.0 41 216099.5 42 212773.0 43 209319.0 44 205740.5 45 200962.5 46 197637.0 47 192603.5 48 186578.5 49 182234.5 50 178761.5 51 172479.5 52 161828.0 53 152444.0 54 144578.5 55 137091.0 56 128244.0 57 117109.0 58 106695.5 59 97079.5 60 85443.0 61 72298.0 62 61273.0 63 51016.0 64 40858.0 65 32220.0 66 25299.5 67 19436.5 68 14715.0 69 11183.0 70 8511.0 71 6371.5 72 4579.5 73 3207.0 74 2306.0 75 1668.0 76 1230.5 77 910.0 78 639.5 79 414.0 80 284.5 81 226.5 82 176.5 83 144.5 84 116.5 85 86.5 86 69.0 87 56.0 88 45.0 89 35.0 90 32.5 91 34.0 92 25.0 93 18.5 94 22.0 95 21.0 96 16.5 97 18.0 98 20.0 99 23.0 100 47.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02012701721580569 2 0.04464387769267188 3 0.04636668410455977 4 0.049298768094022824 5 0.053108820735697976 6 0.04949755344924065 7 0.0474103072194534 8 0.04669799302992283 9 0.04651577312097315 10-11 0.04704586740155404 12-13 0.04826342770226327 14-15 0.04648264222843684 16-17 0.04268915503302984 18-19 0.04567921808443143 20-21 0.0450580138493757 22-23 0.04636668410455977 24-25 0.042871374941979526 26-27 0.043235814759878886 28-29 0.043542275515839717 30-31 0.043641668193448634 32-33 0.043351772883755954 34-35 0.04350086190016933 36-37 0.04065160514204705 38-39 0.04104089312934864 40-41 0.042175626198717106 42-43 0.0421093644136445 44-45 0.04101604495994641 46-47 0.0397902019361031 48-49 0.03992272550624832 50-51 0.04128937482337093 52-53 0.041918861781560735 54-55 0.040817259604728574 56-57 0.03913586680851106 58-59 0.040071814522661696 60-61 0.03943404484133781 62-63 0.039740505597298637 64-65 0.036593070806349604 66-67 0.037570432136170624 68-69 0.03887910239135469 70-71 0.04015464175400246 72-73 0.036882966116042276 74-75 0.037305384995880174 76-77 0.03783547927646107 78-79 0.03824961543316488 80-81 0.03850637985032126 82-83 0.03702377240932157 84-85 0.038365573557041954 86-87 0.03844011806524864 88-89 0.03926010765552221 90-91 0.03991444278311425 92-93 0.037992851016008515 94-95 0.03923525948611998 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6036662.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.756866318189985 #Duplication Level Percentage of deduplicated Percentage of total 1 77.56108455255529 49.45051699311099 2 13.772150267555105 17.561382870450707 3 4.004383175308747 7.6592076838490675 4 1.6654772406752494 4.247424391588793 5 0.8502146647024209 2.710351135959849 6 0.5108117698151174 1.9540654633116286 7 0.3222528130811463 1.4382080666992718 8 0.23022564163776224 1.174277236553466 9 0.17718842491156014 1.0167280848195288 >10 0.8522195382740153 9.570815507411021 >50 0.04095358061892285 1.7743314663022247 >100 0.012908308144452606 1.2977363988019799 >500 5.2010240453158224E-5 0.021321835822923716 >1k 7.801247979222692E-5 0.1236328653185699 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.656544626815283E-5 0.0 1.656544626815283E-5 0.0 3 0.0 1.656544626815283E-5 0.0 3.313089253630566E-5 1.656544626815283E-5 4 0.0 1.656544626815283E-5 0.0 3.313089253630566E-5 1.656544626815283E-5 5 0.0 1.656544626815283E-5 0.0 3.313089253630566E-5 1.656544626815283E-5 6 0.0 1.656544626815283E-5 0.0 3.313089253630566E-5 1.656544626815283E-5 7 0.0 1.656544626815283E-5 0.0 3.313089253630566E-5 1.656544626815283E-5 8 0.0 1.656544626815283E-5 0.0 4.969633880445849E-5 1.656544626815283E-5 9 0.0 1.656544626815283E-5 0.0 9.939267760891697E-5 1.656544626815283E-5 10-11 0.0 1.656544626815283E-5 0.0 9.939267760891697E-5 1.656544626815283E-5 12-13 0.0 1.656544626815283E-5 0.0 1.159581238770698E-4 1.656544626815283E-5 14-15 0.0 3.313089253630566E-5 0.0 1.159581238770698E-4 1.656544626815283E-5 16-17 0.0 4.969633880445849E-5 0.0 1.3252357014522265E-4 1.656544626815283E-5 18-19 0.0 4.969633880445849E-5 0.0 1.3252357014522265E-4 1.656544626815283E-5 20-21 0.0 4.969633880445849E-5 0.0 1.9050263208375757E-4 1.656544626815283E-5 22-23 0.0 4.969633880445849E-5 0.0 4.5554977237420287E-4 1.656544626815283E-5 24-25 0.0 4.969633880445849E-5 0.0 0.0010933194536980868 1.656544626815283E-5 26-27 0.0 4.969633880445849E-5 0.0 0.002368858816345855 1.656544626815283E-5 28-29 0.0 4.969633880445849E-5 0.0 0.006634461230395209 6.626178507261133E-5 30-31 0.0 4.969633880445849E-5 0.0 0.019812273736710784 6.626178507261133E-5 32-33 0.0 4.969633880445849E-5 0.0 0.05159308240216199 6.626178507261133E-5 34-35 0.0 4.969633880445849E-5 0.0 0.10131426937602271 6.626178507261133E-5 36-37 0.0 5.79790619385349E-5 0.0 0.17549433776481108 6.626178507261133E-5 38-39 0.0 8.282723134076414E-5 0.0 0.2746965127416443 8.282723134076416E-5 40-41 0.0 9.939267760891697E-5 0.0 0.38243817526971036 8.282723134076416E-5 42-43 0.0 9.939267760891697E-5 0.0 0.4908507383716365 8.282723134076416E-5 44-45 8.282723134076416E-6 1.159581238770698E-4 0.0 0.6179988212028436 8.282723134076416E-5 46-47 1.656544626815283E-5 1.3252357014522265E-4 0.0 0.7671706648475598 8.282723134076416E-5 48-49 1.656544626815283E-5 1.4908901641337547E-4 0.0 0.9233662577099728 8.282723134076416E-5 50-51 1.656544626815283E-5 1.4908901641337547E-4 0.0 1.0815082905088937 8.282723134076416E-5 52-53 1.656544626815283E-5 1.4908901641337547E-4 0.0 1.2353515900012293 8.282723134076416E-5 54-55 1.656544626815283E-5 1.4908901641337547E-4 0.0 1.4039381366722203 8.282723134076416E-5 56-57 1.656544626815283E-5 1.4908901641337547E-4 0.0 1.5983253659058598 8.282723134076416E-5 58-59 1.656544626815283E-5 1.4908901641337547E-4 0.0 1.8007468365795534 8.282723134076416E-5 60-61 1.656544626815283E-5 1.4908901641337547E-4 0.0 2.003731532426364 8.282723134076416E-5 62-63 1.656544626815283E-5 1.4908901641337547E-4 0.0 2.2052170553859067 8.282723134076416E-5 64-65 1.656544626815283E-5 1.4908901641337547E-4 0.0 2.423268024613603 9.110995447484057E-5 66-67 1.656544626815283E-5 1.4908901641337547E-4 0.0 2.665976660611444 1.159581238770698E-4 68-69 1.656544626815283E-5 1.4908901641337547E-4 0.0 2.9207615069387685 1.3252357014522265E-4 70-71 1.656544626815283E-5 1.4908901641337547E-4 0.0 3.1871090347612636 1.3252357014522265E-4 72-73 1.656544626815283E-5 1.6565446268152831E-4 0.0 3.4682329406549517 1.6565446268152831E-4 74-75 1.656544626815283E-5 1.7393718581560472E-4 0.0 3.7488681658837284 1.6565446268152831E-4 76-77 1.656544626815283E-5 1.8221990894968113E-4 0.0 4.041339402471101 1.6565446268152831E-4 78-79 1.656544626815283E-5 1.8221990894968113E-4 0.0 4.359280012695758 1.6565446268152831E-4 80-81 1.656544626815283E-5 1.8221990894968113E-4 0.0 4.696179776174317 1.6565446268152831E-4 82-83 1.656544626815283E-5 1.8221990894968113E-4 0.0 5.041917205236934 1.6565446268152831E-4 84-85 1.656544626815283E-5 1.9050263208375754E-4 0.0 5.381633094581078 1.6565446268152831E-4 86-87 1.656544626815283E-5 1.9878535521783395E-4 0.0 5.74082994873657 1.6565446268152831E-4 88 1.656544626815283E-5 2.153508014859868E-4 0.0 6.025730776379397 1.6565446268152831E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6235 0.0 43.961506 1 GTATCAA 10350 0.0 34.205433 1 TCAACGC 10905 0.0 31.340763 4 ATCAACG 10980 0.0 31.126688 3 CAACGCA 11280 0.0 30.257925 5 AACGCAG 12090 0.0 28.458372 6 TATCAAC 12410 0.0 27.918787 2 GTACATG 14900 0.0 24.48589 1 ACGCAGA 13965 0.0 24.435484 7 CGCAGAG 13865 0.0 24.204918 8 TACATGG 14865 0.0 23.33954 2 ACATGGG 15570 0.0 21.346745 3 GTGGTAT 3640 0.0 21.182777 1 GCAGAGT 15805 0.0 20.430902 9 TATAACG 565 0.0 19.969337 2 CATGGGG 11030 0.0 18.62545 4 CAGAGTA 15050 0.0 18.020409 10-11 AGAGTAC 13900 0.0 17.905087 10-11 GTCTAGG 1525 0.0 17.572983 1 TCTGTCG 1835 0.0 17.161846 8 >>END_MODULE