FastQCFastQC Report
Wed 25 May 2016
SRR2049365_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049365_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6036662
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168190.2786142407840625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161490.26751539178440004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96650.1601050381816971No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103350.058.505591
GTATCAA185700.039.6503221
ATCAACG225700.032.0880743
TCAACGC226950.031.8285054
CAACGCA234450.030.9305935
TATCAAC235850.030.866552
AACGCAG243600.029.7924826
GTGGTAT42600.028.8069841
TGGTATC44800.025.9114362
ACGCAGA280200.025.682747
CGCAGAG280850.025.5228148
ATAACGC5600.024.3378663
GTACATG143700.023.8199831
TATAACG5850.023.2977892
GCAGAGT306700.023.1255049
TACATGG144800.022.557392
CAGAGTA299250.021.225810-11
ACATGGG149050.021.1259063
GAGTACT234550.021.03671312-13
AGAGTAC285400.020.58316210-11
Produced by FastQC (version 0.11.5)