##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049365_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6036662
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85873534082246	31.0	31.0	34.0	30.0	34.0
2	32.0699452777048	33.0	31.0	34.0	30.0	34.0
3	32.03513580849814	34.0	31.0	34.0	30.0	34.0
4	35.73448356061678	37.0	35.0	37.0	33.0	37.0
5	35.816925479677344	37.0	35.0	37.0	35.0	37.0
6	35.85608255025708	37.0	35.0	37.0	35.0	37.0
7	35.80463143372943	37.0	35.0	37.0	35.0	37.0
8	35.80307577267039	37.0	35.0	37.0	35.0	37.0
9	37.552927429099064	39.0	37.0	39.0	35.0	39.0
10-11	37.513620772539525	39.0	37.0	39.0	35.0	39.0
12-13	37.4605433433245	39.0	37.0	39.0	34.5	39.0
14-15	38.75246245358776	40.0	38.0	41.0	35.0	41.0
16-17	38.77943406471987	40.0	38.0	41.0	35.0	41.0
18-19	38.85379519343637	40.0	38.0	41.0	35.0	41.0
20-21	38.881412111527865	40.0	38.0	41.0	35.0	41.0
22-23	38.84048601694115	40.0	38.0	41.0	35.0	41.0
24-25	38.813563605184456	40.0	38.0	41.0	35.0	41.0
26-27	38.69435343572325	40.0	38.0	41.0	34.5	41.0
28-29	38.60840278948863	40.0	38.0	41.0	34.0	41.0
30-31	38.495954055403466	40.0	38.0	41.0	34.0	41.0
32-33	38.338948577873005	40.0	38.0	41.0	34.0	41.0
34-35	38.22319594835689	40.0	38.0	41.0	34.0	41.0
36-37	38.09823475291477	40.0	38.0	41.0	34.0	41.0
38-39	37.99166575832803	40.0	38.0	41.0	33.0	41.0
40-41	37.87442265278394	40.0	37.5	41.0	33.0	41.0
42-43	37.73480484744715	40.0	37.0	41.0	33.0	41.0
44-45	37.550842750513446	40.0	37.0	41.0	33.0	41.0
46-47	37.31258674081802	40.0	37.0	41.0	32.0	41.0
48-49	37.10065065759852	40.0	37.0	41.0	31.5	41.0
50-51	36.95276338148467	40.0	37.0	41.0	31.0	41.0
52-53	36.812733262190264	40.0	36.0	41.0	31.0	41.0
54-55	36.670722329658346	40.0	36.0	41.0	31.0	41.0
56-57	36.53008202215065	39.0	36.0	41.0	31.0	41.0
58-59	36.34045462542048	39.0	35.0	41.0	30.0	41.0
60-61	36.1601618576624	39.0	35.0	41.0	30.0	41.0
62-63	35.947169644416064	39.0	35.0	41.0	30.0	41.0
64-65	35.71653407131292	38.0	35.0	40.5	29.5	41.0
66-67	35.44977787393099	38.0	35.0	40.0	29.0	41.0
68-69	35.15602968991804	37.0	35.0	40.0	29.0	41.0
70-71	34.82153324138406	37.0	34.0	39.5	29.0	41.0
72-73	34.51845415562441	36.0	34.0	39.0	28.5	41.0
74-75	34.21469663201286	36.0	34.0	39.0	28.0	41.0
76-77	33.89100640387022	35.0	34.0	38.5	28.0	40.0
78-79	33.55491743615926	35.0	34.0	37.0	28.0	39.0
80-81	33.23507594097533	35.0	34.0	37.0	27.5	39.0
82-83	32.93375262686564	35.0	34.0	36.5	27.0	39.0
84-85	32.65489462553975	35.0	33.0	36.0	27.0	37.5
86-87	32.416956092622044	35.0	33.0	36.0	26.5	37.0
88-89	32.184353704083485	35.0	33.0	35.0	26.0	37.0
90-91	31.98736081297909	35.0	33.0	35.0	26.0	36.0
92-93	31.799008210166477	35.0	33.0	35.0	26.0	36.0
94-95	31.63202726937503	35.0	33.0	35.0	25.0	36.0
96-97	31.424683459169984	35.0	33.0	35.0	25.0	36.0
98-99	31.274097837513516	35.0	33.0	35.0	24.5	35.0
100	31.158955230556224	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.20618536513260466
1101	2	0.22629664104341884
1101	3	0.3603130746312786
1101	4	0.22757244268880328
1101	5	0.18071530820189707
1101	6	0.18238547799773386
1101	7	0.0656767283669879
1101	8	0.1453278259320001
1101	9	0.21006849596273014
1101	10-11	0.18231117137983688
1101	12-13	0.17072742904731797
1101	14-15	0.3299891456383932
1101	16-17	0.25488999029995085
1101	18-19	0.30763164982920443
1101	20-21	0.3243658017401785
1101	22-23	0.29734081496366116
1101	24-25	0.2886867416930059
1101	26-27	0.3559121045241582
1101	28-29	0.3830529469682844
1101	30-31	0.31399202272424986
1101	32-33	0.3611812449644489
1101	34-35	0.3660468189964874
1101	36-37	0.38855978628057386
1101	38-39	0.3790398065754914
1101	40-41	0.4042923245313048
1101	42-43	0.39971215452835906
1101	44-45	0.40386453563377245
1101	46-47	0.44729803809973845
1101	48-49	0.45076763090951744
1101	50-51	0.40676455062464356
1101	52-53	0.39438001137823875
1101	54-55	0.3384561489594091
1101	56-57	0.34858345608020613
1101	58-59	0.40446565495995657
1101	60-61	0.3893785487185468
1101	62-63	0.38443072923187316
1101	64-65	0.3266845307563102
1101	66-67	0.3425190448245772
1101	68-69	0.3852068157931825
1101	70-71	0.37089203820608674
1101	72-73	0.29349879179376615
1101	74-75	0.33804966479331
1101	76-77	0.2887010680061408
1101	78-79	0.3093888028503997
1101	80-81	0.4072212787103311
1101	82-83	0.36690925297834553
1101	84-85	0.38927936818370057
1101	86-87	0.3308189011164302
1101	88-89	0.43631711604993484
1101	90-91	0.43280129259910183
1101	92-93	0.42024624201838634
1101	94-95	0.42891635419045926
1101	96-97	0.4639927436748472
1101	98-99	0.5122274791394901
1101	100	0.49321252352541833
1102	1	0.23601348500508834
1102	2	0.20321873290783543
1102	3	0.3921574785593762
1102	4	0.20529550400004126
1102	5	0.17154100908935987
1102	6	0.1525618048674886
1102	7	0.13630704339087885
1102	8	0.16717820516998927
1102	9	0.137243799155371
1102	10-11	0.2585289890626967
1102	12-13	0.2328469217053879
1102	14-15	0.2952342633968996
1102	16-17	0.22262241186109577
1102	18-19	0.27312805840786325
1102	20-21	0.27048726977796633
1102	22-23	0.29204205125064675
1102	24-25	0.2787958044601311
1102	26-27	0.33988569629112675
1102	28-29	0.33693983795076576
1102	30-31	0.3138593259496716
1102	32-33	0.33867333222178786
1102	34-35	0.34454103377770195
1102	36-37	0.4153392652777299
1102	38-39	0.36181362595296207
1102	40-41	0.380472793855013
1102	42-43	0.43911160916107406
1102	44-45	0.45724446949561326
1102	46-47	0.46735667951309523
1102	48-49	0.3812390675386226
1102	50-51	0.47146596150327014
1102	52-53	0.5275651080930999
1102	54-55	0.35958631985612044
1102	56-57	0.4500531400230088
1102	58-59	0.49547306655333756
1102	60-61	0.41728666648167945
1102	62-63	0.4276925856968674
1102	64-65	0.40176056897976764
1102	66-67	0.4337325743229101
1102	68-69	0.4572222696477226
1102	70-71	0.43075748862223406
1102	72-73	0.4936630932187569
1102	74-75	0.38332127059815946
1102	76-77	0.4234491518628616
1102	78-79	0.4751332954639267
1102	80-81	0.4867284456972598
1102	82-83	0.4970999818770707
1102	84-85	0.4311920802012068
1102	86-87	0.44071636593888996
1102	88-89	0.37317354007817016
1102	90-91	0.42108808894579397
1102	92-93	0.45915364751888177
1102	94-95	0.4205521389210993
1102	96-97	0.4613864895171602
1102	98-99	0.5125664292166121
1102	100	0.5259606423004719
1103	1	0.12002692455686415
1103	2	0.15252639573510152
1103	3	0.2902116464016231
1103	4	0.1755901133447182
1103	5	0.15728101956263885
1103	6	0.056698625833689675
1103	7	0.09541413051350389
1103	8	0.13425115943225308
1103	9	0.1564518952271996
1103	10-11	0.16740307854212944
1103	12-13	0.16004936770076483
1103	14-15	0.21456197032836144
1103	16-17	0.2001113811964501
1103	18-19	0.20876296683832862
1103	20-21	0.2445104468526509
1103	22-23	0.2362692254392016
1103	24-25	0.171998097982609
1103	26-27	0.2562252426234508
1103	28-29	0.25339155340115127
1103	30-31	0.28097421297312763
1103	32-33	0.2517471051570652
1103	34-35	0.2689884810730163
1103	36-37	0.2522342158510966
1103	38-39	0.27975811512206405
1103	40-41	0.32280914531546046
1103	42-43	0.3227514683994883
1103	44-45	0.32880173414490343
1103	46-47	0.30402677600541494
1103	48-49	0.3048857742109661
1103	50-51	0.2926197010422129
1103	52-53	0.30085099889861766
1103	54-55	0.2875462298015421
1103	56-57	0.29416891478840057
1103	58-59	0.3168155781793942
1103	60-61	0.31110115252530335
1103	62-63	0.27374534452233235
1103	64-65	0.3165351911803924
1103	66-67	0.29822705337058864
1103	68-69	0.2936923540638432
1103	70-71	0.2807256664212687
1103	72-73	0.3221848288598608
1103	74-75	0.3150676790144047
1103	76-77	0.2790934834223435
1103	78-79	0.30487025311273186
1103	80-81	0.3485113520413208
1103	82-83	0.3518501166483503
1103	84-85	0.329420528961073
1103	86-87	0.318134857284754
1103	88-89	0.3541955176233813
1103	90-91	0.35426519321836025
1103	92-93	0.3852122888767866
1103	94-95	0.33692529662173243
1103	96-97	0.42563853910473526
1103	98-99	0.4166070518946299
1103	100	0.44579093149724613
1104	1	0.15432710871892397
1104	2	0.17934499610342414
1104	3	0.2962063094602172
1104	4	0.18707205418735384
1104	5	0.17267640423584396
1104	6	0.16300993488769677
1104	7	0.1492744868107181
1104	8	0.13405149393069138
1104	9	0.12690844801405632
1104	10-11	0.15834413728606478
1104	12-13	0.12750154194063157
1104	14-15	0.22311000280100757
1104	16-17	0.19800784641237357
1104	18-19	0.17728158975711494
1104	20-21	0.20868105984922636
1104	22-23	0.2136623926508321
1104	24-25	0.2254802643298106
1104	26-27	0.26333450898192723
1104	28-29	0.3071215398708844
1104	30-31	0.2771688234139802
1104	32-33	0.3396833155528327
1104	34-35	0.31428553635921475
1104	36-37	0.3457446172492311
1104	38-39	0.3497573164600567
1104	40-41	0.36967533654333096
1104	42-43	0.3675188228550752
1104	44-45	0.3922883166231941
1104	46-47	0.3584779072866553
1104	48-49	0.3564711182933493
1104	50-51	0.33563352259292856
1104	52-53	0.326774628018768
1104	54-55	0.27801631286239115
1104	56-57	0.24166041138703775
1104	58-59	0.2796672243627256
1104	60-61	0.28667501466684797
1104	62-63	0.2877818292819754
1104	64-65	0.22586708692210777
1104	66-67	0.26049027357580457
1104	68-69	0.2424617098724653
1104	70-71	0.30077443178376484
1104	72-73	0.3109260435777941
1104	74-75	0.32693838033360123
1104	76-77	0.22638179101057432
1104	78-79	0.2628864706494696
1104	80-81	0.2852796367032582
1104	82-83	0.3678621153704853
1104	84-85	0.2988235526014691
1104	86-87	0.3038470288937276
1104	88-89	0.3312069713761545
1104	90-91	0.2983929133671879
1104	92-93	0.38511128162305397
1104	94-95	0.4287585078067657
1104	96-97	0.46224427255833334
1104	98-99	0.45991215017703624
1104	100	0.44766543820600546
1105	1	0.14783018416627414
1105	2	0.1741936752903399
1105	3	0.26617428903450246
1105	4	0.1419065113899194
1105	5	0.16888099294808967
1105	6	0.16101218451845511
1105	7	0.17267167729631439
1105	8	0.15203622547239348
1105	9	0.17075939176638855
1105	10-11	0.1899525062478773
1105	12-13	0.1356862572550881
1105	14-15	0.22151286801906167
1105	16-17	0.2338676140134126
1105	18-19	0.22459034297624925
1105	20-21	0.22955075637614897
1105	22-23	0.20314022905417062
1105	24-25	0.22473759272603644
1105	26-27	0.27394104028238786
1105	28-29	0.2477379428913835
1105	30-31	0.24077865719475966
1105	32-33	0.29138236844238463
1105	34-35	0.26153484790216197
1105	36-37	0.27939754056833266
1105	38-39	0.2908672618140571
1105	40-41	0.30738790975043884
1105	42-43	0.2959821716817359
1105	44-45	0.3179343867694442
1105	46-47	0.32196625506745136
1105	48-49	0.3328873756179931
1105	50-51	0.34423278377289535
1105	52-53	0.361995524239596
1105	54-55	0.3229713294175838
1105	56-57	0.2447648546741661
1105	58-59	0.3396464608245324
1105	60-61	0.33761384991567667
1105	62-63	0.379990794983712
1105	64-65	0.3242170782189575
1105	66-67	0.36481216204291655
1105	68-69	0.3741285598016191
1105	70-71	0.3874777497838977
1105	72-73	0.3961015725069643
1105	74-75	0.38204350415195165
1105	76-77	0.3578861938081914
1105	78-79	0.36687189267882303
1105	80-81	0.3915957820529101
1105	82-83	0.38244724766751403
1105	84-85	0.3753757152924493
1105	86-87	0.34772233971578714
1105	88-89	0.3929184532180159
1105	90-91	0.4096605957592985
1105	92-93	0.43719707222398796
1105	94-95	0.454522317188502
1105	96-97	0.46249562892637996
1105	98-99	0.5203248846696908
1105	100	0.498830782398727
1106	1	0.13996186599814564
1106	2	0.16219084162395347
1106	3	0.2374799091395019
1106	4	0.15884942075092567
1106	5	0.12238200777867547
1106	6	0.1181837785196862
1106	7	0.13368869448273557
1106	8	0.11375064185259731
1106	9	0.13734222250604944
1106	10-11	0.12003333107285385
1106	12-13	0.1534074967104999
1106	14-15	0.2133879371791778
1106	16-17	0.21775971892319745
1106	18-19	0.23344670159681158
1106	20-21	0.22919986896894784
1106	22-23	0.20847700357808208
1106	24-25	0.2151642410512551
1106	26-27	0.25003452354883393
1106	28-29	0.24343559280942628
1106	30-31	0.24393416675205515
1106	32-33	0.20551603292462062
1106	34-35	0.16924508924113724
1106	36-37	0.21430533189727186
1106	38-39	0.2374019691645941
1106	40-41	0.18795670065435388
1106	42-43	0.18463142603017246
1106	44-45	0.15150948821030852
1106	46-47	0.1829162215933522
1106	48-49	0.1547468251383961
1106	50-51	0.1439265144366857
1106	52-53	0.14638867990103677
1106	54-55	0.1662690418238384
1106	56-57	0.13052956248517233
1106	58-59	0.1472034469548973
1106	60-61	0.07445282683529086
1106	62-63	0.11643265118225088
1106	64-65	0.08209169462891452
1106	66-67	0.06310491059083034
1106	68-69	0.06765305712960412
1106	70-71	0.17502732508350505
1106	72-73	0.16078528745950393
1106	74-75	0.13620952894037686
1106	76-77	0.14679530939520902
1106	78-79	0.18974173086282775
1106	80-81	0.17675908913493288
1106	82-83	0.1340577894657855
1106	84-85	0.14663283592192755
1106	86-87	0.13124669962758162
1106	88-89	0.16967799514478088
1106	90-91	0.17820092005666766
1106	92-93	0.22774244090076223
1106	94-95	0.18426404949550346
1106	96-97	0.21498083679729163
1106	98-99	0.24782966831995878
1106	100	0.3066339243113134
1107	1	0.12362273488479403
1107	2	0.09937266498253194
1107	3	0.18447356300633544
1107	4	0.12309025745346247
1107	5	0.11059030604904052
1107	6	0.08940261993384269
1107	7	0.0545565898822673
1107	8	0.11536522551546824
1107	9	0.12585348589061596
1107	10-11	0.12066172268980324
1107	12-13	0.08911815184488603
1107	14-15	0.15163952371247547
1107	16-17	0.07541562521385714
1107	18-19	0.15133139769316983
1107	20-21	0.12789115036479615
1107	22-23	0.11024449340717979
1107	24-25	0.13911693926510793
1107	26-27	0.18434482183185708
1107	28-29	0.2041812581832474
1107	30-31	0.17585401439423975
1107	32-33	0.2138054452798599
1107	34-35	0.23224485513652837
1107	36-37	0.23494107355545424
1107	38-39	0.21548392476064748
1107	40-41	0.23123362743022824
1107	42-43	0.2641341962680954
1107	44-45	0.20198191553770073
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2216	76-77	-0.5826313387991533
2216	78-79	-0.7922161061009305
2216	80-81	-0.9140445395853334
2216	82-83	-0.818669054289245
2216	84-85	-0.9363770592920275
2216	86-87	-0.7867314486861687
2216	88-89	-1.1378779484296118
2216	90-91	-0.9348801150878572
2216	92-93	-1.007661259050625
2216	94-95	-0.8993909453717883
2216	96-97	-0.9450389163170776
2216	98-99	-1.097666886781333
2216	100	-1.059314435853178
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	388.0
3	41.0
4	54.0
5	113.0
6	378.0
7	937.0
8	2192.0
9	3896.0
10	5598.0
11	8151.0
12	11574.0
13	16446.0
14	18843.0
15	22740.0
16	27549.0
17	36846.0
18	36683.0
19	21073.0
20	16333.0
21	15862.0
22	16951.0
23	18667.0
24	21488.0
25	26487.0
26	33626.0
27	42532.0
28	55545.0
29	72492.0
30	93425.0
31	120823.0
32	155945.0
33	203472.0
34	277008.0
35	401606.0
36	642410.0
37	1183517.0
38	1698354.0
39	723296.0
40	3321.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.17291280050035	20.39232797928337	14.186271354578729	23.24848786563755
2	15.781784701545323	20.76455498088182	39.88498941964947	23.56867089792339
3	17.685618310251595	25.592140159578257	31.256942992667142	25.46529853750301
4	12.467720151918419	17.519254435523212	38.41313931658578	31.59988609597259
5	12.192499762285847	37.47261317595718	35.70782992322578	14.627057138531194
6	29.968101576666044	38.43029475561163	17.57961601958168	14.021987648140644
7	25.699981214783936	33.09353745497098	22.947135353942294	18.2593459763028
8	24.331131435977664	36.121387294727576	20.960101460885674	18.58737980840909
9	24.27147652129604	16.576926122416662	22.357836168399025	36.79376118788827
10-11	23.27879215367698	26.93014782010323	29.22944832756911	20.56161169865068
12-13	24.19723713945359	24.6204364707138	29.42942335776585	21.75290303206676
14-15	21.47813059291806	26.705597738614053	27.267482041369952	24.548789627097932
16-17	20.307439773835277	29.93302424419323	27.850225836728974	21.909310145242518
18-19	20.107181803709633	28.028831842249165	31.056740160409923	20.80724619363128
20-21	21.32345998296407	27.481855014383793	30.74418378128016	20.450501221371972
22-23	20.95272188504177	27.50504335011634	30.783527386492732	20.75870737834916
24-25	20.808764326128706	27.469830759708174	30.809247209567353	20.912157704595767
26-27	20.5890779404387	27.289627060816553	30.98885318018449	21.132441818560263
28-29	20.70987709795016	27.342022333132032	31.008318953775003	20.939781615142802
30-31	21.05982447247319	27.824856791669568	30.50767311725358	20.607645618603666
32-33	20.543222000023853	27.54637176128209	30.9910731916123	20.91933304708176
34-35	20.677124548529243	27.124457419244653	31.026317703219615	21.17210032900649
36-37	21.2280571846305	27.690350801776546	30.21642607820852	20.865165935384436
38-39	21.100801016724454	27.740918148099098	30.357870408217945	20.800410426958504
40-41	20.79115555959753	27.476849332518977	30.576457045471074	21.155538062412422
42-43	20.75112960414324	27.55477064347844	30.349385451613053	21.34471430076526
44-45	21.0392776119848	27.739050416249423	30.103219586376206	21.118452385389567
46-47	21.06709628663433	28.131068656754	29.55943467348546	21.24240038312621
48-49	21.052793747478923	27.88887959374516	29.601613821013633	21.456712837762286
50-51	21.120419465688816	27.76018978794798	29.455240935674066	21.66414981068914
52-53	21.062456696604766	27.943324267833546	29.350609550290745	21.643609485270947
54-55	21.210906908777368	28.394141932608953	29.116067608886247	21.278883549727436
56-57	20.95625586937871	28.120985444354936	29.097913453986806	21.82484523227955
58-59	20.749046118452103	27.652203183520136	29.609695839630284	21.989054858397477
60-61	21.159043893233466	28.09324810722772	29.099104337373987	21.64860366216483
62-63	20.999343504948065	28.098501756699918	29.105168917021707	21.79698582133031
64-65	20.929013065563712	27.81384553505995	29.350016698052823	21.907124701323518
66-67	20.887743057249512	27.899444162505294	29.036091957240924	22.17672082300427
68-69	21.35826112122035	28.05254949572658	28.86290387574439	21.726285507308678
70-71	21.413513363080604	27.801346843736706	28.921847480515627	21.863292312667063
72-73	21.050310646978968	27.995078351289095	29.101579615660818	21.85303138607112
74-75	21.15400050077854	27.956173346240153	28.928475555074417	21.961350597906883
76-77	21.2576921245152	28.074810168782577	28.64068217067105	22.026815536031176
78-79	21.31814556108034	28.201247921993694	28.678450111696723	21.802156405229244
80-81	21.168711087049957	27.93769140427341	28.780907651250843	22.112689857425792
82-83	21.00681952571194	28.00614952530525	28.86499081803745	22.122040130945365
84-85	21.194856196513623	27.908385815742886	28.6790435531247	22.21771443461879
86-87	21.307767535271985	28.009844026091375	28.589959719571816	22.092428719064824
88-89	21.05679963184074	27.91548041437133	28.68174997573104	22.345969978056885
90-91	20.91511069151279	27.78568436042244	28.67526187943565	22.623943068629117
92-93	21.10530899315215	27.994358644994698	28.468342463082458	22.431989898770695
94-95	20.991288442884024	27.75123742908964	28.687903970681894	22.56957015734444
96-97	20.953791745948863	27.772281541448006	28.672859927367956	22.601066785235172
98-99	20.925787093718036	27.784181913797706	28.625800520453737	22.664230472030518
100	21.06332432102461	27.783609834675683	28.44726606664492	22.705799777654786
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1554.0
1	1114.0
2	1934.0
3	2935.0
4	1680.5
5	854.0
6	1781.0
7	4195.0
8	6755.5
9	7556.5
10	5754.5
11	3696.0
12	3397.0
13	3711.5
14	4284.0
15	4947.5
16	5682.5
17	6502.5
18	7508.5
19	8724.0
20	10501.5
21	13145.5
22	16857.0
23	22266.0
24	29548.5
25	38308.5
26	50029.5
27	64313.5
28	80228.0
29	98592.5
30	116978.0
31	132466.5
32	145647.5
33	158037.0
34	169938.5
35	180056.0
36	188477.0
37	197323.5
38	205698.5
39	209509.5
40	211327.5
41	212926.0
42	210576.5
43	207173.5
44	203962.5
45	200873.0
46	198065.0
47	193234.5
48	186678.0
49	181966.0
50	178555.0
51	172211.0
52	161062.5
53	151035.5
54	143616.0
55	136202.5
56	127859.0
57	117251.0
58	107087.0
59	97296.5
60	85154.0
61	71562.0
62	60191.5
63	49826.5
64	39511.0
65	30942.0
66	23992.5
67	18081.0
68	13428.5
69	9984.5
70	7286.0
71	5167.0
72	3642.0
73	2449.0
74	1700.5
75	1175.0
76	747.5
77	475.5
78	296.0
79	176.5
80	97.0
81	40.5
82	18.0
83	14.5
84	10.0
85	7.5
86	4.5
87	1.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04101604495994641
2	0.0
3	0.0
4	1.656544626815283E-5
5	0.0
6	0.0
7	0.0
8	8.282723134076415E-4
9	0.0
10-11	0.0
12-13	0.04350086190016933
14-15	1.6565446268152831E-4
16-17	0.0
18-19	4.969633880445849E-5
20-21	1.3252357014522265E-4
22-23	0.0
24-25	1.4080629327929906E-4
26-27	1.2424084701114624E-4
28-29	4.224188798378971E-4
30-31	3.313089253630566E-5
32-33	2.319162477541396E-4
34-35	4.969633880445849E-5
36-37	8.282723134076416E-5
38-39	3.5615709476528585E-4
40-41	1.8221990894968113E-4
42-43	5.632251731171962E-4
44-45	2.816125865585981E-4
46-47	1.4908901641337547E-4
48-49	9.442304372847114E-4
50-51	4.96963388044585E-4
52-53	6.543351275920368E-4
54-55	4.886806649105085E-4
56-57	5.797906193853491E-4
58-59	1.8221990894968113E-4
60-61	2.319162477541396E-4
62-63	9.77361329821017E-4
64-65	4.96963388044585E-4
66-67	0.0015820001186085952
68-69	0.0017476545812901236
70-71	4.96963388044585E-4
72-73	0.0011098848999662398
74-75	0.001018774945491399
76-77	8.199895902735651E-4
78-79	0.0037603563028706924
80-81	0.003172282960351267
82-83	0.0016316964574130538
84-85	0.003230262022289802
86-87	0.001780785473826429
88-89	0.0023854242626140075
90-91	0.003329654699898719
92-93	0.0027995604193178283
94-95	0.004232471521513049
96-97	0.0034456128237757887
98-99	0.0032882410842283365
100	0.0017062409656197416
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6036662.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.17647755386276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.16163975450972	46.59300844907146
2	14.545693644247498	17.797086014653427
3	4.311299971156473	7.912504377402694
4	1.7881018767381776	4.37559097305172
5	0.8840969170563379	2.704296760086816
6	0.5490757483029027	2.0154312114853754
7	0.3496167038130395	1.4971822903291727
8	0.25018115882611924	1.2244161637860365
9	0.1796708367744374	0.9892466021713598
>10	0.9167477145795676	9.93999290241633
>50	0.046336525946829674	1.9127318824758692
>100	0.016672287536646922	1.6926764327178192
>500	4.334384043904243E-4	0.18433594653475252
>1k	3.5215546263708103E-4	0.4549849094577129
>5k	2.708888173153708E-5	0.16016860859741325
>10k+	5.417776346307416E-5	0.5463464757619783
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16819	0.2786142407840625	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16149	0.26751539178440004	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9665	0.1601050381816971	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.656544626815283E-5	0.0
4	0.0	0.0	0.0	1.656544626815283E-5	0.0
5	0.0	0.0	0.0	1.656544626815283E-5	0.0
6	0.0	0.0	0.0	1.656544626815283E-5	0.0
7	0.0	0.0	0.0	1.656544626815283E-5	0.0
8	0.0	0.0	0.0	3.313089253630566E-5	0.0
9	0.0	0.0	0.0	9.939267760891697E-5	0.0
10-11	0.0	0.0	0.0	9.939267760891697E-5	0.0
12-13	0.0	0.0	0.0	1.159581238770698E-4	0.0
14-15	0.0	3.313089253630566E-5	0.0	1.2424084701114624E-4	0.0
16-17	0.0	4.969633880445849E-5	0.0	1.4908901641337547E-4	0.0
18-19	0.0	4.969633880445849E-5	0.0	1.7393718581560472E-4	8.282723134076416E-6
20-21	0.0	4.969633880445849E-5	0.0	2.484816940222925E-4	1.656544626815283E-5
22-23	0.0	4.969633880445849E-5	0.0	5.218115574468141E-4	1.656544626815283E-5
24-25	0.0	4.969633880445849E-5	0.0	0.001176146685038851	1.656544626815283E-5
26-27	0.0	4.969633880445849E-5	0.0	0.0024682514939547715	1.656544626815283E-5
28-29	0.0	4.969633880445849E-5	0.0	0.006709005738601896	1.656544626815283E-5
30-31	0.0	4.969633880445849E-5	0.0	0.019837121906113014	1.656544626815283E-5
32-33	0.0	6.626178507261133E-5	0.0	0.051783585034245745	1.656544626815283E-5
34-35	0.0	6.626178507261133E-5	0.0	0.10178638459466507	1.656544626815283E-5
36-37	0.0	7.454450820668773E-5	0.0	0.1760824111073305	1.656544626815283E-5
38-39	0.0	8.282723134076416E-5	0.0	0.2755082196087838	3.313089253630566E-5
40-41	0.0	8.282723134076416E-5	0.0	0.38407815445025745	3.313089253630566E-5
42-43	0.0	8.282723134076416E-5	0.0	0.4931947490185801	3.313089253630566E-5
44-45	0.0	8.282723134076416E-5	0.0	0.620864643407234	3.313089253630566E-5
46-47	0.0	8.282723134076416E-5	0.0	0.7706991049026763	3.313089253630566E-5
48-49	0.0	8.282723134076416E-5	0.0	0.9271100485665753	3.313089253630566E-5
50-51	0.0	8.282723134076416E-5	0.0	1.0858981337699545	3.313089253630566E-5
52-53	0.0	8.282723134076416E-5	0.0	1.2402549620966024	3.313089253630566E-5
54-55	0.0	8.282723134076416E-5	0.0	1.409843718266817	3.313089253630566E-5
56-57	0.0	8.282723134076416E-5	0.0	1.6049184135205847	3.313089253630566E-5
58-59	0.0	1.159581238770698E-4	0.0	1.808615423556926	3.313089253630566E-5
60-61	0.0	1.159581238770698E-4	0.0	2.012710004303703	3.313089253630566E-5
62-63	0.0	1.159581238770698E-4	0.0	2.2156947001505136	3.313089253630566E-5
64-65	0.0	1.159581238770698E-4	0.0	2.4354601930669633	4.141361567038207E-5
66-67	0.0	1.159581238770698E-4	0.0	2.6791296249483576	4.969633880445849E-5
68-69	0.0	1.159581238770698E-4	0.0	2.936200502860687	4.969633880445849E-5
70-71	0.0	1.159581238770698E-4	0.0	3.2040554862935844	4.969633880445849E-5
72-73	0.0	1.6565446268152831E-4	0.0	3.4877553190819697	4.969633880445849E-5
74-75	0.0	1.6565446268152831E-4	0.0	3.7705771832181427	4.969633880445849E-5
76-77	0.0	1.6565446268152831E-4	0.0	4.066129592811391	6.626178507261133E-5
78-79	0.0	1.6565446268152831E-4	0.0	4.386737239885221	8.282723134076416E-5
80-81	0.0	1.6565446268152831E-4	0.0	4.727206857034567	8.282723134076416E-5
82-83	0.0	1.6565446268152831E-4	0.0	5.076696359676921	8.282723134076416E-5
84-85	0.0	1.8221990894968113E-4	0.0	5.420603306926908	8.282723134076416E-5
86-87	0.0	1.9050263208375754E-4	0.0	5.783485972877063	8.282723134076416E-5
88	0.0	1.9878535521783395E-4	0.0	6.070523743088482	8.282723134076416E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10335	0.0	58.50559	1
GTATCAA	18570	0.0	39.650322	1
ATCAACG	22570	0.0	32.088074	3
TCAACGC	22695	0.0	31.828505	4
CAACGCA	23445	0.0	30.930593	5
TATCAAC	23585	0.0	30.86655	2
AACGCAG	24360	0.0	29.792482	6
GTGGTAT	4260	0.0	28.806984	1
TGGTATC	4480	0.0	25.911436	2
ACGCAGA	28020	0.0	25.68274	7
CGCAGAG	28085	0.0	25.522814	8
ATAACGC	560	0.0	24.337866	3
GTACATG	14370	0.0	23.819983	1
TATAACG	585	0.0	23.297789	2
GCAGAGT	30670	0.0	23.125504	9
TACATGG	14480	0.0	22.55739	2
CAGAGTA	29925	0.0	21.2258	10-11
ACATGGG	14905	0.0	21.125906	3
GAGTACT	23455	0.0	21.036713	12-13
AGAGTAC	28540	0.0	20.583162	10-11
>>END_MODULE