FastQCFastQC Report
Wed 25 May 2016
SRR2049364_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049364_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3997829
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38750.045.132341
GTATCAA68150.035.6649251
TCAACGC72050.032.3601074
ATCAACG73050.032.045823
CAACGCA75650.030.7580325
AACGCAG80200.029.00946
TATCAAC84250.028.3436722
ACGCAGA95200.024.1917367
CGCAGAG94300.023.9242028
GTACATG99500.023.624551
TCTGTCG9750.022.1748738
TACATGG101050.021.817192
GCAGAGT105200.020.3730969
ACATGGG103700.020.0810873
GTCTCGC11850.018.6464461
TATAACG3601.10958354E-1018.2804892
GTGGTAT25250.018.246571
CATGGGG71900.018.0444014
CTGTCGC12950.017.7841979
AGAGTAC91600.017.72803110-11
Produced by FastQC (version 0.11.5)