##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049364_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3997829
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.90388733485099	31.0	31.0	34.0	28.0	34.0
2	30.991663225215486	31.0	31.0	34.0	28.0	34.0
3	31.193128570531655	31.0	31.0	34.0	28.0	34.0
4	34.793350340897526	37.0	35.0	37.0	32.0	37.0
5	34.78098863157979	37.0	35.0	37.0	32.0	37.0
6	34.7614767915286	37.0	35.0	37.0	32.0	37.0
7	34.75148086624015	37.0	35.0	37.0	32.0	37.0
8	34.697221166788275	37.0	35.0	37.0	32.0	37.0
9	36.2876518730541	39.0	37.0	39.0	32.0	39.0
10-11	36.2446774236717	39.0	37.0	39.0	32.0	39.0
12-13	36.17823136007068	39.0	37.0	39.0	32.0	39.0
14-15	37.39795173830596	40.0	38.0	41.0	32.0	41.0
16-17	37.36381033305827	40.0	38.0	41.0	32.0	41.0
18-19	37.392420486218896	40.0	38.0	41.0	32.0	41.0
20-21	37.474994928497445	40.0	38.0	41.0	32.0	41.0
22-23	37.43169492742186	40.0	38.0	41.0	32.0	41.0
24-25	37.440645285228555	40.0	38.0	41.0	32.0	41.0
26-27	37.436308056197504	40.0	38.0	41.0	32.0	41.0
28-29	37.38314457671902	40.0	38.0	41.0	32.0	41.0
30-31	37.32382287986805	40.0	38.0	41.0	31.5	41.0
32-33	37.27117943263707	40.0	37.5	41.0	31.0	41.0
34-35	37.175110040974744	40.0	37.0	41.0	31.0	41.0
36-37	37.09184322290923	40.0	37.0	41.0	31.0	41.0
38-39	36.984730337390616	40.0	37.0	41.0	30.0	41.0
40-41	36.88244157016221	40.0	37.0	41.0	30.0	41.0
42-43	36.7472705811079	40.0	37.0	41.0	30.0	41.0
44-45	36.62536579228376	40.0	37.0	41.0	30.0	41.0
46-47	36.50438037744986	40.0	36.0	41.0	30.0	41.0
48-49	36.38244882409928	40.0	36.0	41.0	30.0	41.0
50-51	36.246134964752116	40.0	36.0	41.0	29.5	41.0
52-53	36.10719830688106	39.5	35.5	41.0	29.0	41.0
54-55	35.96946830392196	39.0	35.0	41.0	29.0	41.0
56-57	35.84841860419743	39.0	35.0	41.0	29.0	41.0
58-59	35.650887018929524	39.0	35.0	41.0	28.0	41.0
60-61	35.43711136719455	39.0	35.0	40.5	28.0	41.0
62-63	35.225617704008855	38.5	35.0	40.0	28.0	41.0
64-65	34.97809586152884	38.0	34.0	40.0	28.0	41.0
66-67	34.716349173513926	37.5	34.0	40.0	27.5	41.0
68-69	34.42735582237259	37.0	34.0	40.0	26.5	41.0
70-71	34.133571120725776	36.5	34.0	39.5	26.0	41.0
72-73	33.784849602121554	36.0	34.0	39.0	26.0	41.0
74-75	33.43730147037304	35.5	33.5	39.0	26.0	40.5
76-77	33.101469072339015	35.0	33.0	38.5	26.0	40.0
78-79	32.78064707119789	35.0	33.0	37.0	25.5	39.0
80-81	32.42275382463832	35.0	33.0	37.0	25.0	39.0
82-83	32.10707061257497	35.0	33.0	36.0	24.5	39.0
84-85	31.809111895481273	35.0	33.0	36.0	24.0	37.5
86-87	31.48248511879823	35.0	32.0	36.0	22.5	37.0
88-89	31.227360524924904	35.0	32.0	35.0	21.0	37.0
90-91	31.017492118847507	35.0	32.0	35.0	20.0	36.0
92-93	30.773319719277637	35.0	32.0	35.0	18.5	36.0
94-95	30.567751146935	35.0	32.0	35.0	15.5	36.0
96-97	30.311145874423342	35.0	32.0	35.0	4.5	36.0
98-99	30.104011452215687	35.0	32.0	35.0	2.0	35.0
100	29.985271506109942	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4822207074798648
1101	2	0.5063943622840483
1101	3	0.5174901616477321
1101	4	0.5186178018481087
1101	5	0.5130657093584148
1101	6	0.5527617035642365
1101	7	0.4636962579701418
1101	8	0.5864297136664831
1101	9	0.7061085463637298
1101	10-11	0.6403934297865135
1101	12-13	0.6598455664551466
1101	14-15	0.7400644552988709
1101	16-17	0.7447643364070444
1101	18-19	0.7097876067125668
1101	20-21	0.7136753226133408
1101	22-23	0.7282194667428854
1101	24-25	0.7186513258229539
1101	26-27	0.6628327626288026
1101	28-29	0.6964760697421823
1101	30-31	0.7056556137321337
1101	32-33	0.7208877690214024
1101	34-35	0.7776157156308514
1101	36-37	0.766551229303964
1101	38-39	0.8061361773072164
1101	40-41	0.8652825710879455
1101	42-43	0.7970643328828189
1101	44-45	0.7561591519370694
1101	46-47	0.6719660124365348
1101	48-49	0.6776688037429821
1101	50-51	0.7004278383234848
1101	52-53	0.668098988864898
1101	54-55	0.6853662223205816
1101	56-57	0.6547166577955466
1101	58-59	0.7149750627185085
1101	60-61	0.7176945672924759
1101	62-63	0.7473931472474291
1101	64-65	0.6623573973998944
1101	66-67	0.6946734157934458
1101	68-69	0.6922879246809117
1101	70-71	0.6926701835604732
1101	72-73	0.7425077363681751
1101	74-75	0.7774669288043086
1101	76-77	0.7694542471413328
1101	78-79	0.74115457347785
1101	80-81	0.7382060623478282
1101	82-83	0.7081593441042671
1101	84-85	0.7374387660383626
1101	86-87	0.7239329225891247
1101	88-89	0.7264871545762475
1101	90-91	0.7683695621175488
1101	92-93	0.7302470233965366
1101	94-95	0.7045694901207895
1101	96-97	0.7202535399486152
1101	98-99	0.7943075861370765
1101	100	0.7709503196873655
1102	1	0.28462163469575685
1102	2	0.31949308189879844
1102	3	0.32299694991392514
1102	4	0.35656902935551216
1102	5	0.29569414788158355
1102	6	0.3871831107002137
1102	7	0.46839290305640446
1102	8	0.44769729896371047
1102	9	0.533475776150155
1102	10-11	0.5544040677937829
1102	12-13	0.5603465596220119
1102	14-15	0.6632546087031486
1102	16-17	0.6478584901859605
1102	18-19	0.613062364664728
1102	20-21	0.548417196791398
1102	22-23	0.570191517583666
1102	24-25	0.5940628559242995
1102	26-27	0.6040500066590724
1102	28-29	0.5807943358172452
1102	30-31	0.5674745334631766
1102	32-33	0.5862685089990052
1102	34-35	0.6025913840184813
1102	36-37	0.583597718878643
1102	38-39	0.6062644109531306
1102	40-41	0.6315660197379032
1102	42-43	0.5831325238362126
1102	44-45	0.5508602067622164
1102	46-47	0.5528759796171485
1102	48-49	0.5916869344039313
1102	50-51	0.6238943613809553
1102	52-53	0.6065047706959916
1102	54-55	0.6944285869552544
1102	56-57	0.6980665764300156
1102	58-59	0.7579118707699877
1102	60-61	0.6923647149211618
1102	62-63	0.7026182195398221
1102	64-65	0.646593556478301
1102	66-67	0.7271347915539295
1102	68-69	0.6874447826053682
1102	70-71	0.7239030763101582
1102	72-73	0.7525382923465003
1102	74-75	0.7370655254927172
1102	76-77	0.7562784936808598
1102	78-79	0.6723914466998124
1102	80-81	0.6540744882271383
1102	82-83	0.5852699114776598
1102	84-85	0.6084974545898199
1102	86-87	0.6370542762618889
1102	88-89	0.6077662910479091
1102	90-91	0.6563031116451583
1102	92-93	0.6641279448368458
1102	94-95	0.7092550523234742
1102	96-97	0.7612193898379793
1102	98-99	0.7890636651377463
1102	100	0.7262578892188039
1103	1	0.21128058205941258
1103	2	0.23933627192716855
1103	3	0.32246792370022703
1103	4	0.32622444419513386
1103	5	0.3416428187911009
1103	6	0.3189444318626897
1103	7	0.3399394364984616
1103	8	0.42041571312216774
1103	9	0.47723337696060497
1103	10-11	0.44739465904461895
1103	12-13	0.47059330827421064
1103	14-15	0.48562718551460904
1103	16-17	0.5199779668926467
1103	18-19	0.523875478830206
1103	20-21	0.527816282996902
1103	22-23	0.5134809816402495
1103	24-25	0.4957774614741979
1103	26-27	0.49027862425258917
1103	28-29	0.4724637947594772
1103	30-31	0.5364423620735295
1103	32-33	0.5858001610329424
1103	34-35	0.4983715603957606
1103	36-37	0.5287762497620179
1103	38-39	0.5151817447611933
1103	40-41	0.5496796534099531
1103	42-43	0.5597915692626785
1103	44-45	0.41070255194178884
1103	46-47	0.4396748701496378
1103	48-49	0.48885611763881087
1103	50-51	0.4306045578761939
1103	52-53	0.49866010821686046
1103	54-55	0.4893925790468643
1103	56-57	0.47326056352042656
1103	58-59	0.5540860635056504
1103	60-61	0.4618929800166143
1103	62-63	0.47528254828859673
1103	64-65	0.5271950737362232
1103	66-67	0.5581987051198638
1103	68-69	0.5258183128890579
1103	70-71	0.5349251396873953
1103	72-73	0.5516184330439913
1103	74-75	0.5227666104445206
1103	76-77	0.5718372140668393
1103	78-79	0.4800203710036257
1103	80-81	0.4820654604142618
1103	82-83	0.5119727457190777
1103	84-85	0.4874890370293379
1103	86-87	0.5129136572140176
1103	88-89	0.4713848689712563
1103	90-91	0.4648089588286055
1103	92-93	0.4579598546250985
1103	94-95	0.4516814128129134
1103	96-97	0.46418130903272115
1103	98-99	0.4655605448546609
1103	100	0.3971330695977926
1104	1	0.15443064599250889
1104	2	0.05309445602069118
1104	3	0.12551851698246352
1104	4	0.1520578334660101
1104	5	0.21667083646141805
1104	6	0.21257348354053818
1104	7	0.2408496205901045
1104	8	0.24969369159708776
1104	9	0.2928122402188791
1104	10-11	0.2932072289861054
1104	12-13	0.24555297456410585
1104	14-15	0.35827260103749126
1104	16-17	0.309690667540238
1104	18-19	0.2391892069136361
1104	20-21	0.25242543796284167
1104	22-23	0.28650420151189593
1104	24-25	0.3853974925770345
1104	26-27	0.28615377481456505
1104	28-29	0.36899980441891245
1104	30-31	0.37906385138609267
1104	32-33	0.3453655697071909
1104	34-35	0.30695153626358973
1104	36-37	0.28973620497129815
1104	38-39	0.392433734690556
1104	40-41	0.40063937743677513
1104	42-43	0.3874612747855224
1104	44-45	0.32424929210491626
1104	46-47	0.3404010324223492
1104	48-49	0.28114701604433634
1104	50-51	0.3236515167825118
1104	52-53	0.32656708380118005
1104	54-55	0.3366301188994356
1104	56-57	0.2902057062714789
1104	58-59	0.37532371871473913
1104	60-61	0.34581574254600156
1104	62-63	0.2716144015457118
1104	64-65	0.3620828352423118
1104	66-67	0.39980252223553236
1104	68-69	0.3566332586116374
1104	70-71	0.37159646794676604
1104	72-73	0.3285393069183584
1104	74-75	0.30626889375263744
1104	76-77	0.33742451489786873
1104	78-79	0.3080866805853262
1104	80-81	0.34633941607980034
1104	82-83	0.23834550301459245
1104	84-85	0.2834072555913387
1104	86-87	0.34581718090400315
1104	88-89	0.31078789074888036
1104	90-91	0.27459661363870325
1104	92-93	0.21658194794582286
1104	94-95	0.2664681094480237
1104	96-97	0.2839066554778107
1104	98-99	0.3596513306731559
1104	100	0.38302938508931916
1105	1	0.18949739149568856
1105	2	0.1848234775803057
1105	3	0.21636855445598613
1105	4	0.27382908005525053
1105	5	0.22964068598390952
1105	6	0.2884394977490601
1105	7	0.23345883795447975
1105	8	0.3624167375885392
1105	9	0.36626429767229496
1105	10-11	0.3626344450849146
1105	12-13	0.3253214802008344
1105	14-15	0.3290961125308698
1105	16-17	0.3249122802255897
1105	18-19	0.35486678484510037
1105	20-21	0.38552659066632344
1105	22-23	0.3347917793760402
1105	24-25	0.29984457300823664
1105	26-27	0.3232349885752015
1105	28-29	0.352872123491359
1105	30-31	0.32323183822772705
1105	32-33	0.3463977464475718
1105	34-35	0.4063629972148348
1105	36-37	0.3737187708080114
1105	38-39	0.3796707047880261
1105	40-41	0.40976260922509766
1105	42-43	0.359823122028196
1105	44-45	0.3655795011488365
1105	46-47	0.29681692848830465
1105	48-49	0.32349456388478615
1105	50-51	0.39569441835505614
1105	52-53	0.3387038627163719
1105	54-55	0.2761440121515051
1105	56-57	0.3099119963255177
1105	58-59	0.31437680826075365
1105	60-61	0.2525466714405482
1105	62-63	0.23402241470856922
1105	64-65	0.26211719532021505
1105	66-67	0.2924464362663315
1105	68-69	0.2576828117342842
1105	70-71	0.21526666841888442
1105	72-73	0.24530957740089576
1105	74-75	0.31694212969220814
1105	76-77	0.34472789671490744
1105	78-79	0.24316474387743625
1105	80-81	0.32017201490219804
1105	82-83	0.27639370753923487
1105	84-85	0.2557237896047333
1105	86-87	0.29127724692023094
1105	88-89	0.28222444213023934
1105	90-91	0.2769041523239437
1105	92-93	0.33569758124834337
1105	94-95	0.30059239307546193
1105	96-97	0.2175838654058282
1105	98-99	0.2687662459201654
1105	100	0.2960757524016344
1106	1	0.17813388106687
1106	2	0.16916653582571328
1106	3	0.19911684785487438
1106	4	0.21137173663615272
1106	5	0.234137247103277
1106	6	0.3535702346790828
1106	7	0.3754392655947285
1106	8	0.3686842985849168
1106	9	0.418356885935097
1106	10-11	0.3850599472669103
1106	12-13	0.4257710552230378
1106	14-15	0.43464555632284174
1106	16-17	0.49371023298000694
1106	18-19	0.49933842438499454
1106	20-21	0.4345705426557842
1106	22-23	0.4307774994731872
1106	24-25	0.44268691698820106
1106	26-27	0.40555451998463354
1106	28-29	0.46242327827680896
1106	30-31	0.406789136912316
1106	32-33	0.4200404055963176
1106	34-35	0.48896235579500313
1106	36-37	0.41046026077788866
1106	38-39	0.3856786434733763
1106	40-41	0.5272660318877911
1106	42-43	0.4985043461048164
1106	44-45	0.37844823983849807
1106	46-47	0.3766058519004005
1106	48-49	0.4314214236441316
1106	50-51	0.42911109391906876
1106	52-53	0.4780884726950063
1106	54-55	0.4493524357534895
1106	56-57	0.37049069324045547
1106	58-59	0.4372699279316379
1106	60-61	0.4334670669962932
1106	62-63	0.4094531025792989
1106	64-65	0.5100406222806626
1106	66-67	0.5324057697627467
1106	68-69	0.5599362955521485
1106	70-71	0.5249108736786425
1106	72-73	0.526545682336419
1106	74-75	0.537531152033786
1106	76-77	0.6069721092727391
1106	78-79	0.4780713758016546
1106	80-81	0.5219698419675183
1106	82-83	0.4705203778189002
1106	84-85	0.5148719283925196
1106	86-87	0.5873745329818512
1106	88-89	0.5347823503393592
1106	90-91	0.5790438402095432
1106	92-93	0.5471060964817234
1106	94-95	0.6208162412970921
1106	96-97	0.5438846461648552
1106	98-99	0.515419199411145
1106	100	0.440898645656457
1107	1	0.1759004101805992
1107	2	0.06111524248224498
1107	3	0.07440506325896834
1107	4	0.0912898188842064
1107	5	0.17456643133458272
1107	6	0.2207645463815311
1107	7	0.1886380490028401
1107	8	0.21606553478286372
1107	9	0.2911580326565897
1107	10-11	0.18523398808392244
1107	12-13	0.24008347754514858
1107	14-15	0.3218913498071103
1107	16-17	0.3636437819940852
1107	18-19	0.3311225227286485
1107	20-21	0.25178541848762137
1107	22-23	0.2785557001198029
1107	24-25	0.2742856735067889
1107	26-27	0.23565540331588863
1107	28-29	0.3026064075391659
1107	30-31	0.3085650360702772
1107	32-33	0.3119757998241752
1107	34-35	0.2971867901094214
1107	36-37	0.3084940706414656
1107	38-39	0.38097435406594116
1107	40-41	0.42924844571958687
1107	42-43	0.371662043599855
1107	44-45	0.3046084060877092
1107	46-47	0.37440522539579746
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2215	100	-0.27086161464834646
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2216	2	-1.0481586781832455
2216	3	-1.1319397632356427
2216	4	-1.3369261796611482
2216	5	-1.2608026809344253
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2216	7	-2.5359946029868965
2216	8	-3.35279518258713
2216	9	-2.818107105320415
2216	10-11	-2.059552086857309
2216	12-13	-1.8562098225568064
2216	14-15	-2.0703792280320314
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2216	36-37	-2.387506220634947
2216	38-39	-2.2507329658320003
2216	40-41	-2.8295865528500883
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2216	54-55	-2.5119440919947778
2216	56-57	-2.5159489752074364
2216	58-59	-3.525382303295231
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2216	62-63	-3.432944481550507
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2216	66-67	-3.035924909063368
2216	68-69	-3.4403926484423906
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2216	80-81	-3.6037689633137546
2216	82-83	-3.832057693265238
2216	84-85	-3.876930919134246
2216	86-87	-3.4635397457272425
2216	88-89	-3.3769835392184895
2216	90-91	-3.509353545625892
2216	92-93	-3.638534533547368
2216	94-95	-3.5150031955566483
2216	96-97	-3.728098678548047
2216	98-99	-3.4321939663585574
2216	100	-3.073481457310095
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	60964.0
3	10965.0
4	6886.0
5	5719.0
6	4829.0
7	5219.0
8	5785.0
9	6644.0
10	7701.0
11	8744.0
12	9749.0
13	10782.0
14	12293.0
15	12798.0
16	13040.0
17	13610.0
18	12823.0
19	11906.0
20	11762.0
21	12378.0
22	13980.0
23	16644.0
24	20757.0
25	25359.0
26	31021.0
27	35799.0
28	43143.0
29	54728.0
30	66812.0
31	79263.0
32	101598.0
33	134661.0
34	184754.0
35	266306.0
36	434288.0
37	780970.0
38	1054502.0
39	407399.0
40	1248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.45535538933923	20.50425974846056	13.947076693985865	23.093308168214342
2	15.589120117232955	20.668163775797982	40.45595431197959	23.28676179498947
3	17.93762505968331	25.74778058162825	30.7801972527159	25.534397105972534
4	12.559772810766063	17.5468047259685	39.34482392734269	30.548598535922743
5	12.60865082280819	37.71708892374747	35.56849794335492	14.105762310089423
6	29.810884271003896	38.46575238249682	18.23988660413778	13.483476742361509
7	25.949948999050047	32.82314031717686	23.297524226702475	17.929386457070613
8	23.99774175643502	36.62798990797357	21.279040726962236	18.095227608629173
9	24.1619989054128	17.62875976563722	22.355829928076062	35.85341140087392
10-11	23.076546739329277	27.410001944442985	29.484700086561492	20.028751229666245
12-13	23.68469423824673	25.074734810449296	29.33636138977781	21.904209561526166
14-15	21.329988677502516	27.46285591449406	26.56249902246644	24.644656385536983
16-17	20.757033818960153	30.6055126958263	27.038552235756413	21.598901249457136
18-19	20.60157162717829	29.508706035798603	29.12480188245916	20.76492045456394
20-21	21.889266342598678	28.91762541994332	28.496299493878368	20.696808743579638
22-23	21.50935865176356	28.89482949974162	28.52557597718739	21.070235871307432
24-25	21.304562651105453	28.870598618754013	28.574778203504376	21.25006052663616
26-27	21.067900815956893	28.706870859000638	28.730230743610065	21.494997581432408
28-29	21.167608677400914	28.80140525237976	28.733439844170032	21.297546226049295
30-31	21.484703197118325	29.27121897838805	28.218729773477968	21.025348051015662
32-33	20.990144375409848	28.96594454980294	28.77113177463928	21.272779300147928
34-35	21.11755771571237	28.50055697212362	28.847502496458787	21.534382815705225
36-37	21.655515664265057	28.971811892442407	28.197528825263014	21.17514361802953
38-39	21.42444076933144	29.04981646539042	28.389009293464945	21.136733471813194
40-41	21.18052149542065	28.782668535108353	28.605024773534858	21.43178519593614
42-43	21.06539696362303	28.789252817044147	28.542506524932378	21.602843694400445
44-45	21.337045639064637	28.90896572381391	28.454916017069465	21.29907262005198
46-47	21.23547379797328	29.189430357431963	28.190934994331023	21.38416085026373
48-49	21.129164194588945	28.885036437445528	28.440850676663242	21.54494869130228
50-51	21.196235400453205	28.68443333508082	28.447034497651973	21.672296766814007
52-53	21.159900200552297	28.73252924736648	28.476524470846098	21.631046081235127
54-55	21.370386654118263	29.181567060593423	28.18780014008101	21.260246145207304
56-57	21.091502818214234	28.93012955653006	28.271574509080217	21.706793116175486
58-59	20.94339915366354	28.496405284610727	28.625950664655132	21.934244897070602
60-61	21.340605381494136	28.96640935824117	28.160502704184477	21.53248255608022
62-63	21.230072241642915	28.94042253979296	28.149517930290692	21.679987288273434
64-65	21.16681757201325	28.68982339132544	28.417380542581146	21.725978494080163
66-67	21.117987866061256	28.7727261125905	28.116709713806998	21.99257630754125
68-69	21.60176561553822	28.92574205397939	27.995190599398452	21.47730173108394
70-71	21.578939466355042	28.660770364513088	28.167362728931085	21.59292744020079
72-73	21.296015489840173	28.807564558933407	28.40465455884833	21.49176539237809
74-75	21.352747097800435	28.826983027385715	28.256358177598713	21.563911697215136
76-77	21.409596179934724	28.939614347665128	27.995983356402178	21.654806115997975
78-79	21.506600714777683	29.008813672985145	28.090412557966992	21.39417305427018
80-81	21.435264140303975	28.786594472483213	28.179340581027468	21.59880080618534
82-83	21.28930287654024	28.83706661632155	28.29633205055881	21.577298456579403
84-85	21.382853915633447	28.765349755283466	28.15628277593214	21.69551355315095
86-87	21.609016848161854	28.903164169423977	28.021845483155715	21.465973499258446
88-89	21.372624358612327	28.752422074783635	28.146126100404235	21.728827466199803
90-91	21.329359990331053	28.619417616149338	28.1991412554466	21.852081138073
92-93	21.506222137256355	28.856481472213822	27.950773400701035	21.686522989828784
94-95	21.44645758034666	28.615349467558556	28.106004342007672	21.832188610087112
96-97	21.41678646085163	28.595695313631474	28.16950149693746	21.818016728579437
98-99	21.433683131519633	28.701377672731876	28.057228060529855	21.80771113521864
100	21.587391556767436	28.66475779729448	27.91162403394442	21.83622661199366
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3293.0
1	2427.0
2	1833.5
3	2118.0
4	1964.5
5	1593.5
6	1324.5
7	1177.5
8	1133.5
9	1106.0
10	968.0
11	889.5
12	899.5
13	957.5
14	1053.0
15	1155.0
16	1350.0
17	1734.0
18	2313.0
19	3058.0
20	4205.5
21	6033.5
22	8674.0
23	12603.5
24	17749.5
25	24438.0
26	33420.5
27	43503.5
28	55657.5
29	69340.0
30	81900.0
31	92800.5
32	101100.5
33	109013.5
34	117841.5
35	124342.0
36	129080.0
37	135109.0
38	140129.5
39	141756.0
40	142487.5
41	142253.0
42	139809.0
43	137620.5
44	135121.0
45	131846.5
46	129248.0
47	126192.0
48	122316.0
49	119128.5
50	116515.0
51	112139.5
52	105512.5
53	99616.0
54	95046.0
55	90558.0
56	84519.0
57	77347.0
58	70435.5
59	63521.0
60	55496.5
61	46045.5
62	38040.5
63	31507.5
64	25233.5
65	19766.0
66	15373.5
67	11749.5
68	8840.5
69	6559.5
70	4893.5
71	3794.5
72	2848.0
73	2018.5
74	1463.5
75	1061.0
76	705.0
77	474.0
78	353.0
79	248.0
80	153.5
81	103.0
82	88.0
83	68.0
84	52.5
85	37.5
86	32.0
87	29.0
88	22.5
89	19.0
90	14.5
91	9.5
92	5.5
93	7.5
94	7.5
95	8.5
96	11.0
97	9.5
98	7.0
99	8.5
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.019060345002249974
2	0.04209784860733163
3	0.043448581717727294
4	0.04665031946088739
5	0.050052165812995
6	0.047926011842927746
7	0.04564977641614987
8	0.04509947774154422
9	0.04507446416542578
10-11	0.0456747899922683
12-13	0.04668783982506505
14-15	0.045086970953485
16-17	0.0413599481118377
18-19	0.04451165870276093
20-21	0.043873812511740745
22-23	0.04487435555647828
24-25	0.041384961687956134
26-27	0.041572563508844425
28-29	0.04198528751479866
30-31	0.04211035539539085
32-33	0.04213536897150929
34-35	0.04173515175361427
36-37	0.038808563347756994
38-39	0.03952145026713249
40-41	0.040747115496935965
42-43	0.04049697973575157
44-45	0.03928382129400732
46-47	0.03792058139555244
48-49	0.038220744308973695
50-51	0.04003422857756047
52-53	0.04038441864321861
54-55	0.039271314505948104
56-57	0.03762041848213118
58-59	0.03867098867910559
60-61	0.03808316964032228
62-63	0.03799562212390775
64-65	0.03488143189716219
66-67	0.03589448172995894
68-69	0.037245214840354604
70-71	0.038495893646276515
72-73	0.035094047294168915
74-75	0.035531784876241576
76-77	0.0364572871926238
78-79	0.037057613019466314
80-81	0.03695755871499256
82-83	0.03535668984341252
84-85	0.036782463682163494
86-87	0.03722020126423616
88-89	0.037858047455256345
90-91	0.03827077146121057
92-93	0.036344726100090825
94-95	0.03804564927614463
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3997829.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.34284963168209
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.84260245413675	56.05856425811058
2	12.332087967310239	17.1028424312374
3	3.213144844413144	6.684258593728655
4	1.291349575519464	3.581834377487307
5	0.6652573537104385	2.3065420322356838
6	0.39879403823978277	1.6592109016603518
7	0.26572454692039016	1.2898268110383229
8	0.18463909398522252	1.024272074427784
9	0.1353010581737155	0.8443944838752682
>10	0.6403627223251839	7.550136395329127
>50	0.02477415798784619	1.150206691215551
>100	0.005853814624619503	0.6077085511778695
>500	7.224843552377512E-5	0.040680488693001664
>1k	3.612421774165162E-5	0.09952190978315081
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.501357611843828E-5	0.0
5	0.0	0.0	0.0	2.501357611843828E-5	0.0
6	0.0	0.0	0.0	2.501357611843828E-5	0.0
7	0.0	0.0	0.0	2.501357611843828E-5	0.0
8	0.0	0.0	0.0	2.501357611843828E-5	0.0
9	0.0	0.0	0.0	5.002715223687656E-5	0.0
10-11	1.250678805921914E-5	0.0	0.0	7.504072835531485E-5	0.0
12-13	2.501357611843828E-5	0.0	0.0	7.504072835531485E-5	0.0
14-15	2.501357611843828E-5	0.0	0.0	7.504072835531485E-5	0.0
16-17	2.501357611843828E-5	0.0	0.0	1.2506788059219142E-4	0.0
18-19	2.501357611843828E-5	0.0	0.0	1.7509503282906797E-4	0.0
20-21	2.501357611843828E-5	2.501357611843828E-5	0.0	2.5013576118438285E-4	0.0
22-23	2.501357611843828E-5	5.002715223687656E-5	0.0	5.127783104279848E-4	0.0
24-25	2.501357611843828E-5	5.002715223687656E-5	0.0	0.0011381177133889418	0.0
26-27	2.501357611843828E-5	5.002715223687656E-5	0.0	0.0024138100954292944	0.0
28-29	2.501357611843828E-5	5.002715223687656E-5	0.0	0.006040778632602845	0.0
30-31	2.501357611843828E-5	5.002715223687656E-5	0.0	0.016246317688925663	0.0
32-33	2.501357611843828E-5	5.002715223687656E-5	0.0	0.040196816822330325	0.0
34-35	2.501357611843828E-5	5.002715223687656E-5	0.0	0.07725442984179665	0.0
36-37	2.501357611843828E-5	7.504072835531485E-5	0.0	0.13482317527838233	0.0
38-39	2.501357611843828E-5	1.0005430447375312E-4	0.0	0.20671219304277394	0.0
40-41	2.501357611843828E-5	1.1256109253297227E-4	0.0	0.2890068584724359	0.0
42-43	2.501357611843828E-5	1.7509503282906797E-4	0.0	0.37535372323328486	0.0
44-45	2.501357611843828E-5	1.7509503282906797E-4	0.0	0.47403228102052386	0.0
46-47	2.501357611843828E-5	1.7509503282906797E-4	0.0	0.5876314369624114	0.0
48-49	3.7520364177657425E-5	1.7509503282906797E-4	0.0	0.7065334710414077	0.0
50-51	5.002715223687656E-5	1.7509503282906797E-4	0.0	0.8281369713411955	0.0
52-53	5.002715223687656E-5	1.7509503282906797E-4	0.0	0.9463011049246979	0.0
54-55	5.002715223687656E-5	1.7509503282906797E-4	0.0	1.0781476646449861	2.501357611843828E-5
56-57	5.002715223687656E-5	1.7509503282906797E-4	0.0	1.230355275325683	2.501357611843828E-5
58-59	5.002715223687656E-5	2.0010860894750625E-4	0.0	1.388441076394213	2.501357611843828E-5
60-61	5.002715223687656E-5	2.0010860894750625E-4	0.0	1.5472397643821183	2.501357611843828E-5
62-63	5.002715223687656E-5	2.0010860894750625E-4	0.0	1.7050003889611087	2.501357611843828E-5
64-65	5.002715223687656E-5	2.0010860894750625E-4	0.0	1.8790448515932021	2.501357611843828E-5
66-67	5.002715223687656E-5	2.0010860894750625E-4	0.0	2.0778027274303126	2.501357611843828E-5
68-69	5.002715223687656E-5	2.0010860894750625E-4	0.0	2.284202250771606	2.501357611843828E-5
70-71	5.002715223687656E-5	2.0010860894750625E-4	0.0	2.5014326525721833	2.501357611843828E-5
72-73	5.002715223687656E-5	2.0010860894750625E-4	0.0	2.7337087204080013	2.501357611843828E-5
74-75	5.002715223687656E-5	2.0010860894750625E-4	0.0	2.965572064237865	2.501357611843828E-5
76-77	5.002715223687656E-5	2.0010860894750625E-4	0.0	3.2101423047358955	2.501357611843828E-5
78-79	5.002715223687656E-5	2.0010860894750625E-4	0.0	3.4816896870776617	2.501357611843828E-5
80-81	5.002715223687656E-5	2.0010860894750625E-4	0.0	3.771747115747072	2.501357611843828E-5
82-83	5.002715223687656E-5	2.1261539700672538E-4	0.0	4.069421178344546	2.501357611843828E-5
84-85	5.002715223687656E-5	2.2512218506594455E-4	0.0	4.374574300201434	2.501357611843828E-5
86-87	5.002715223687656E-5	2.2512218506594455E-4	0.0	4.6886947890967825	2.501357611843828E-5
88	5.002715223687656E-5	2.5013576118438285E-4	0.0	4.942807708883997	2.501357611843828E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3875	0.0	45.13234	1
GTATCAA	6815	0.0	35.664925	1
TCAACGC	7205	0.0	32.360107	4
ATCAACG	7305	0.0	32.04582	3
CAACGCA	7565	0.0	30.758032	5
AACGCAG	8020	0.0	29.0094	6
TATCAAC	8425	0.0	28.343672	2
ACGCAGA	9520	0.0	24.191736	7
CGCAGAG	9430	0.0	23.924202	8
GTACATG	9950	0.0	23.62455	1
TCTGTCG	975	0.0	22.174873	8
TACATGG	10105	0.0	21.81719	2
GCAGAGT	10520	0.0	20.373096	9
ACATGGG	10370	0.0	20.081087	3
GTCTCGC	1185	0.0	18.646446	1
TATAACG	360	1.10958354E-10	18.280489	2
GTGGTAT	2525	0.0	18.24657	1
CATGGGG	7190	0.0	18.044401	4
CTGTCGC	1295	0.0	17.784197	9
AGAGTAC	9160	0.0	17.728031	10-11
>>END_MODULE