FastQCFastQC Report
Wed 25 May 2016
SRR2049364_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049364_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3997829
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128950.32255006404726166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123230.30824229850751494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73690.1843250424167717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72100.059.3336071
GTATCAA132000.040.1725851
TCAACGC163150.032.0894554
ATCAACG163200.032.0796243
GTGGTAT29250.031.1795961
TATCAAC168500.031.1542642
CAACGCA168750.031.1081095
AACGCAG175600.029.9683766
TGGTATC30950.029.00262
ACGCAGA199150.026.2356247
CGCAGAG200450.025.971638
GTACATG95050.024.3831251
TATAACG2602.910383E-1123.498182
GCAGAGT218650.023.422659
TACATGG97500.022.6546572
ACATGGG97950.021.9748173
CAGAGTA212100.021.50743910-11
GAGTACT168450.021.11294612-13
AGAGTAC203000.021.05840310-11
AGTACTT175250.019.1536312-13
Produced by FastQC (version 0.11.5)