##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049364_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3997829
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.865613561760647	31.0	31.0	34.0	30.0	34.0
2	32.0783157558765	33.0	31.0	34.0	30.0	34.0
3	32.041295162949694	34.0	31.0	34.0	30.0	34.0
4	35.74120954147864	37.0	35.0	37.0	33.0	37.0
5	35.824882705088186	37.0	35.0	37.0	35.0	37.0
6	35.8622119655443	37.0	35.0	37.0	35.0	37.0
7	35.810402596008984	37.0	35.0	37.0	35.0	37.0
8	35.81097815839547	37.0	35.0	37.0	35.0	37.0
9	37.56019054341744	39.0	37.0	39.0	35.0	39.0
10-11	37.52194441032871	39.0	37.0	39.0	35.0	39.0
12-13	37.4671764350101	39.0	37.0	39.0	35.0	39.0
14-15	38.75701336900603	40.0	38.0	41.0	35.0	41.0
16-17	38.78498430022895	40.0	38.0	41.0	35.0	41.0
18-19	38.86474859229847	40.0	38.0	41.0	35.0	41.0
20-21	38.89121920922581	40.0	38.0	41.0	35.0	41.0
22-23	38.84725272141455	40.0	38.0	41.0	35.0	41.0
24-25	38.82118920043854	40.0	38.0	41.0	35.0	41.0
26-27	38.703807116312376	40.0	38.0	41.0	34.5	41.0
28-29	38.61629687012626	40.0	38.0	41.0	34.0	41.0
30-31	38.496167795070775	40.0	38.0	41.0	34.0	41.0
32-33	38.33290731044274	40.0	38.0	41.0	34.0	41.0
34-35	38.216691484303105	40.0	38.0	41.0	34.0	41.0
36-37	38.088957031428805	40.0	38.0	41.0	34.0	41.0
38-39	37.980365718493715	40.0	38.0	41.0	33.0	41.0
40-41	37.86352767964813	40.0	37.5	41.0	33.0	41.0
42-43	37.71947774654694	40.0	37.0	41.0	33.0	41.0
44-45	37.5260223736433	40.0	37.0	41.0	33.0	41.0
46-47	37.276101103874126	40.0	37.0	41.0	32.0	41.0
48-49	37.054842015503915	40.0	37.0	41.0	31.0	41.0
50-51	36.90502520242862	40.0	37.0	41.0	31.0	41.0
52-53	36.765152161335564	40.0	36.0	41.0	31.0	41.0
54-55	36.62487565125972	40.0	36.0	41.0	31.0	41.0
56-57	36.48888846921667	39.0	36.0	41.0	31.0	41.0
58-59	36.307298036009044	39.0	35.0	41.0	30.0	41.0
60-61	36.128189699959655	39.0	35.0	41.0	30.0	41.0
62-63	35.92215675057638	39.0	35.0	41.0	30.0	41.0
64-65	35.69342710756263	38.5	35.0	40.5	29.0	41.0
66-67	35.425742321645075	38.0	35.0	40.0	29.0	41.0
68-69	35.13385740110445	37.0	35.0	40.0	29.0	41.0
70-71	34.799403126046656	37.0	34.0	39.5	28.5	41.0
72-73	34.503892237511906	36.0	34.0	39.0	28.0	41.0
74-75	34.19986022413665	36.0	34.0	39.0	28.0	41.0
76-77	33.87498614873222	35.5	34.0	38.5	28.0	40.0
78-79	33.541093053254656	35.0	34.0	37.0	28.0	39.5
80-81	33.21958968230007	35.0	34.0	37.0	27.0	39.0
82-83	32.91798536155498	35.0	34.0	36.5	27.0	39.0
84-85	32.6436832340753	35.0	33.0	36.0	26.5	37.5
86-87	32.405998605743264	35.0	33.0	36.0	26.0	37.0
88-89	32.172089026319036	35.0	33.0	35.5	26.0	37.0
90-91	31.97754856448337	35.0	33.0	35.0	26.0	36.0
92-93	31.792869830100287	35.0	33.0	35.0	26.0	36.0
94-95	31.6283266492889	35.0	33.0	35.0	25.0	36.0
96-97	31.428503945516432	35.0	33.0	35.0	25.0	36.0
98-99	31.28002335767738	35.0	33.0	35.0	25.0	35.5
100	31.16922659773592	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18116751111818985
1101	2	0.21413967859613336
1101	3	0.3971203733409112
1101	4	0.23949649964239939
1101	5	0.2625190453926436
1101	6	0.1758076756030107
1101	7	0.07914277658514379
1101	8	0.18515880613586688
1101	9	0.28435358039369163
1101	10-11	0.25272653985184945
1101	12-13	0.23941638991291114
1101	14-15	0.31625100925450056
1101	16-17	0.24879475785211724
1101	18-19	0.3053512330313879
1101	20-21	0.33479167842033064
1101	22-23	0.322442032963842
1101	24-25	0.35588025807587087
1101	26-27	0.37882451595785227
1101	28-29	0.3477190346254062
1101	30-31	0.3957620876849717
1101	32-33	0.4251049703104641
1101	34-35	0.4102256619772504
1101	36-37	0.42917996763051036
1101	38-39	0.4325211511674425
1101	40-41	0.4450244887859398
1101	42-43	0.5260009517784425
1101	44-45	0.5312069664719132
1101	46-47	0.5136593862657506
1101	48-49	0.5410546059194132
1101	50-51	0.46398922151286826
1101	52-53	0.5263528589134623
1101	54-55	0.5131866461592622
1101	56-57	0.48496149085832485
1101	58-59	0.4994099572990862
1101	60-61	0.5331413276954251
1101	62-63	0.5092561446105819
1101	64-65	0.40749065421680086
1101	66-67	0.389837541570472
1101	68-69	0.39525542876803854
1101	70-71	0.46844246982438875
1101	72-73	0.4632735607417402
1101	74-75	0.43139637035491063
1101	76-77	0.45316276730691385
1101	78-79	0.4903673684245433
1101	80-81	0.5390639310531995
1101	82-83	0.49092405095218083
1101	84-85	0.5199922697021506
1101	86-87	0.4044583500585972
1101	88-89	0.4717357615081781
1101	90-91	0.48588698933528107
1101	92-93	0.5514579345447075
1101	94-95	0.6057537457738107
1101	96-97	0.5775848946803102
1101	98-99	0.6049616993675571
1101	100	0.5821529888994625
1102	1	0.22651851462646277
1102	2	0.19999745085848275
1102	3	0.3208788757467502
1102	4	0.20197422655858333
1102	5	0.17385238653082524
1102	6	0.16232230383860724
1102	7	0.18114542242847875
1102	8	0.1852888348967312
1102	9	0.13256428005925613
1102	10-11	0.20781518340836413
1102	12-13	0.21570612146518187
1102	14-15	0.3117377780866235
1102	16-17	0.24238565115594923
1102	18-19	0.25781963315469625
1102	20-21	0.22731806856124592
1102	22-23	0.1996226098201319
1102	24-25	0.1840430359772256
1102	26-27	0.2847674391074193
1102	28-29	0.2937732549016232
1102	30-31	0.2120433119533658
1102	32-33	0.24456612363279362
1102	34-35	0.2704623049561974
1102	36-37	0.2625229789748502
1102	38-39	0.18875226831431036
1102	40-41	0.1798764113150213
1102	42-43	0.2785239077864219
1102	44-45	0.173523913527724
1102	46-47	0.21367585449416993
1102	48-49	0.17583519214150556
1102	50-51	0.28594324315571384
1102	52-53	0.3545648073546417
1102	54-55	0.19861694691906706
1102	56-57	0.25551091310448726
1102	58-59	0.2987566915557309
1102	60-61	0.32795578695607475
1102	62-63	0.3727915064238019
1102	64-65	0.31704542668597924
1102	66-67	0.2838528624862917
1102	68-69	0.30500181024557804
1102	70-71	0.2924375371607084
1102	72-73	0.1709712477751566
1102	74-75	0.2013132097038266
1102	76-77	0.24338430790578514
1102	78-79	0.3077342469928226
1102	80-81	0.21879958086530138
1102	82-83	0.2866655700096672
1102	84-85	0.20755376214511045
1102	86-87	0.22459704220027987
1102	88-89	0.20675900044187046
1102	90-91	0.2632847751000149
1102	92-93	0.36954605907884996
1102	94-95	0.3199339050707479
1102	96-97	0.32902867441190153
1102	98-99	0.3374326750905787
1102	100	0.3723383930539832
1103	1	0.20241615515989508
1103	2	0.2007282148959888
1103	3	0.35855170955820626
1103	4	0.17433297651426471
1103	5	0.1507850875350627
1103	6	0.13534163503517505
1103	7	0.15849119414882296
1103	8	0.11375071976948448
1103	9	0.15347833102627106
1103	10-11	0.1779058613316309
1103	12-13	0.18178346586402228
1103	14-15	0.23900687223521544
1103	16-17	0.21049097966257335
1103	18-19	0.2197153833389507
1103	20-21	0.29895771641016466
1103	22-23	0.25895505857665313
1103	24-25	0.22931128678568058
1103	26-27	0.2771030915004147
1103	28-29	0.2755079708702439
1103	30-31	0.26926719914794006
1103	32-33	0.29436434809252887
1103	34-35	0.22654683811312282
1103	36-37	0.28807205148928716
1103	38-39	0.1821020409971723
1103	40-41	0.33371429539120356
1103	42-43	0.3747759774773982
1103	44-45	0.38143527380218245
1103	46-47	0.32186498898429505
1103	48-49	0.3495362861452733
1103	50-51	0.2971532250442621
1103	52-53	0.30737469148211716
1103	54-55	0.32529470725506826
1103	56-57	0.2369642491634636
1103	58-59	0.29621882611148465
1103	60-61	0.2471090372478102
1103	62-63	0.17311405762578858
1103	64-65	0.23888309351533366
1103	66-67	0.22585427911668887
1103	68-69	0.21936324410485497
1103	70-71	0.33661569163376726
1103	72-73	0.32177235301841733
1103	74-75	0.2596597048259639
1103	76-77	0.243132542963842
1103	78-79	0.1856854433967854
1103	80-81	0.268510227695117
1103	82-83	0.2817970835487458
1103	84-85	0.2438866205024368
1103	86-87	0.23089979527586735
1103	88-89	0.2865882806382416
1103	90-91	0.32357630828183304
1103	92-93	0.3559061260934655
1103	94-95	0.2736639653190309
1103	96-97	0.297537900990541
1103	98-99	0.33319679934068347
1103	100	0.3522334055041867
1104	1	0.15864700926287867
1104	2	0.19374679820992213
1104	3	0.3017041498591482
1104	4	0.18203569418721344
1104	5	0.15052245488092808
1104	6	0.13776720250008623
1104	7	0.16324968007040042
1104	8	0.15737754763642897
1104	9	0.12179195947827992
1104	10-11	0.13115994798022257
1104	12-13	0.0952408868676855
1104	14-15	0.1573376956155741
1104	16-17	0.20116294373236343
1104	18-19	0.21716640610419802
1104	20-21	0.21125434039530688
1104	22-23	0.2052697257067777
1104	24-25	0.24498514901596025
1104	26-27	0.22389115457637843
1104	28-29	0.33897177872923123
1104	30-31	0.21888020617683424
1104	32-33	0.27512603677119785
1104	34-35	0.24297408229624295
1104	36-37	0.20131873853912197
1104	38-39	0.20347182225867755
1104	40-41	0.1526534161327575
1104	42-43	0.17274899144410227
1104	44-45	0.18636283096174822
1104	46-47	0.214819665605809
1104	48-49	0.1554748411352449
1104	50-51	0.14485999486446843
1104	52-53	0.1282758412686391
1104	54-55	0.07642860144251529
1104	56-57	0.04606277196626252
1104	58-59	0.18366893998938139
1104	60-61	0.13085851562039608
1104	62-63	0.15670572170289887
1104	64-65	0.05128104171826209
1104	66-67	0.06275070221953172
1104	68-69	0.061273436645805646
1104	70-71	0.12587078011210906
1104	72-73	0.1331585846130139
1104	74-75	0.1471595317324983
1104	76-77	0.10800947996767718
1104	78-79	0.13700934726337977
1104	80-81	0.12068374761107492
1104	82-83	0.18987591864267017
1104	84-85	0.15379091683468005
1104	86-87	0.16706828116458183
1104	88-89	0.14631686070388383
1104	90-91	0.025775786349310437
1104	92-93	0.18833603380355157
1104	94-95	0.1590448642268143
1104	96-97	0.17726100543525547
1104	98-99	0.2215229274875803
1104	100	0.22157835413239368
1105	1	0.18561630453628197
1105	2	0.20383216827777773
1105	3	0.3195087568583972
1105	4	0.21574638064827667
1105	5	0.18677281390868217
1105	6	0.15252910539205544
1105	7	0.12453941995479312
1105	8	0.15429103092368734
1105	9	0.26507247459335304
1105	10-11	0.2006855601225439
1105	12-13	0.17005731990452233
1105	14-15	0.24062934339588793
1105	16-17	0.24302916999165092
1105	18-19	0.24200311859925705
1105	20-21	0.25519852392131526
1105	22-23	0.2401261454595911
1105	24-25	0.24160937654531267
1105	26-27	0.2692592337554629
1105	28-29	0.3157477319479085
1105	30-31	0.30165880890812957
1105	32-33	0.3362285773413376
1105	34-35	0.34110694163814514
1105	36-37	0.28139215129986184
1105	38-39	0.2608507541735534
1105	40-41	0.2818821880558886
1105	42-43	0.29822886849953534
1105	44-45	0.2753938178617261
1105	46-47	0.30747043762767845
1105	48-49	0.22954431412738074
1105	50-51	0.2256939995048981
1105	52-53	0.310328170124599
1105	54-55	0.29072675547888593
1105	56-57	0.21743548372532473
1105	58-59	0.33619408315168897
1105	60-61	0.33605548441639854
1105	62-63	0.29790554476954156
1105	64-65	0.20114179224334094
1105	66-67	0.22759317598806916
1105	68-69	0.23728311805355418
1105	70-71	0.27154989513113037
1105	72-73	0.24742125660974068
1105	74-75	0.29670285123578566
1105	76-77	0.20808041599790528
1105	78-79	0.30584176937679075
1105	80-81	0.31858985848781884
1105	82-83	0.2851440731053785
1105	84-85	0.23891266651155263
1105	86-87	0.21360558470909297
1105	88-89	0.2578930374173396
1105	90-91	0.2566067616930745
1105	92-93	0.28933518279558257
1105	94-95	0.25593280886025127
1105	96-97	0.3351624770002566
1105	98-99	0.40406900365082876
1105	100	0.3514260141690144
1106	1	0.11714480555193063
1106	2	0.11015933023155355
1106	3	0.24741808771939588
1106	4	0.15057577459709393
1106	5	0.10851229690754138
1106	6	0.1444531080896354
1106	7	0.1482691154222806
1106	8	0.112489000706951
1106	9	0.23333787049372035
1106	10-11	0.1948667632509853
1106	12-13	0.1655472438288328
1106	14-15	0.25382913094077253
1106	16-17	0.27088471132647385
1106	18-19	0.1839002941085397
1106	20-21	0.23062448155733506
1106	22-23	0.19394182307922847
1106	24-25	0.17681306720957934
1106	26-27	0.2674647854292189
1106	28-29	0.26261908713312465
1106	30-31	0.30073269652586276
1106	32-33	0.29785617001677167
1106	34-35	0.2764572020037761
1106	36-37	0.2768511259741899
1106	38-39	0.2906042684000667
1106	40-41	0.3365598495900244
1106	42-43	0.32994766010025955
1106	44-45	0.4008358582957712
1106	46-47	0.4615204645900164
1106	48-49	0.4414778854140451
1106	50-51	0.4260642417334637
1106	52-53	0.462871684905231
1106	54-55	0.4225415032695281
1106	56-57	0.45698862520934114
1106	58-59	0.5233416299843938
1106	60-61	0.45780591452988517
1106	62-63	0.4824603148057989
1106	64-65	0.5060913746987623
1106	66-67	0.4261178157806995
1106	68-69	0.43502753941745453
1106	70-71	0.5205656684366602
1106	72-73	0.5283726014154695
1106	74-75	0.4911138086217761
1106	76-77	0.48595508099592877
1106	78-79	0.48856587100385696
1106	80-81	0.4735762016244536
1106	82-83	0.4898846467612117
1106	84-85	0.4187825445120481
1106	86-87	0.4523828325504198
1106	88-89	0.5139416561212258
1106	90-91	0.45042204737898217
1106	92-93	0.5205352027977241
1106	94-95	0.47539799500849966
1106	96-97	0.525086536460659
1106	98-99	0.5720038912942407
1106	100	0.5093440892067562
1107	1	0.1258034420628107
1107	2	0.06988534298184135
1107	3	0.18544976441846472
1107	4	0.12458202693933629
1107	5	0.08477599838731464
1107	6	0.11514988537032167
1107	7	0.09979964772887229
1107	8	0.08907892285438379
1107	9	0.12395787502471478
1107	10-11	0.09377824062885765
1107	12-13	0.13317681637018808
1107	14-15	0.1503977796683884
1107	16-17	0.11408338357712466
1107	18-19	0.1349098420046957
1107	20-21	0.1159226376139415
1107	22-23	0.09647531323279424
1107	24-25	0.10847018139264009
1107	26-27	0.20963067258217194
1107	28-29	0.17227493835575558
1107	30-31	0.13570722558877435
1107	32-33	0.19613317651472073
1107	34-35	0.11818039135468439
1107	36-37	0.12057334984319112
1107	38-39	0.16956803742122162
1107	40-41	0.19818345244667057
1107	42-43	0.2232521965948422
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2216	74-75	-0.6865136529025193
2216	76-77	-0.6152958097414256
2216	78-79	-0.8317753955697142
2216	80-81	-0.9602173846758504
2216	82-83	-0.8417521219000648
2216	84-85	-0.9812639276796062
2216	86-87	-0.7646938297156822
2216	88-89	-1.068740211811786
2216	90-91	-0.8570464953673742
2216	92-93	-0.9564910751142932
2216	94-95	-0.9553441778582865
2216	96-97	-0.9569847948125627
2216	98-99	-1.2166882742708154
2216	100	-1.1836869868430426
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	223.0
3	21.0
4	32.0
5	102.0
6	241.0
7	635.0
8	1453.0
9	2557.0
10	3855.0
11	5517.0
12	7845.0
13	11144.0
14	12824.0
15	15729.0
16	19202.0
17	26045.0
18	25560.0
19	14753.0
20	10794.0
21	10479.0
22	10948.0
23	11927.0
24	14090.0
25	17516.0
26	22005.0
27	27958.0
28	36040.0
29	47221.0
30	61600.0
31	79254.0
32	101891.0
33	133360.0
34	182564.0
35	263464.0
36	420759.0
37	777203.0
38	1130075.0
39	488859.0
40	2084.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.92149015217467	20.55104981829274	14.590481157020946	22.93697887251164
2	15.65136978094861	20.82202615469546	40.08100396490195	23.445600099453976
3	17.545422778212874	25.804930626097317	31.482412079155964	25.16723451653385
4	12.418549481643273	17.80607760317733	38.69610633379242	31.07926658138698
5	12.097941157563268	37.46846100721166	35.795603063562744	14.637994771662319
6	29.4236696967279	38.72491794921694	17.79490818641818	14.056504167636986
7	25.34100382982864	33.26835640043634	23.16624848136326	18.224391288371763
8	24.204774728488033	36.04754417306727	21.110321231838128	18.637359866606566
9	24.196232505192192	16.714321698101646	22.520548027441894	36.56889776926427
10-11	23.116221329126382	27.090866067558167	29.291898177735966	20.501014425579484
12-13	24.11913909470664	24.775601026896346	29.560130716828002	21.54512916156901
14-15	21.455025988503	26.800406871185352	27.49515955611369	24.24940758419796
16-17	20.130388268232583	30.02824783151055	28.05307580689419	21.788288093362674
18-19	19.904298021863124	28.10021192760318	31.371013381642904	20.624476668890793
20-21	21.19241542873359	27.42845393615756	31.1258129886327	20.253317646476155
22-23	20.80759332127512	27.46060424295286	31.20020891338774	20.531593522384274
24-25	20.623338550750375	27.4406411982508	31.219893804822746	20.716126446176077
26-27	20.41614846861846	27.30533929923949	31.365481456430842	20.91303077571121
28-29	20.51470327063413	27.33657460144509	31.374702400159286	20.774019727761495
30-31	20.89685688117937	27.809497080727702	30.845970506238572	20.447675531854355
32-33	20.413329851439183	27.480899609387503	31.335183840008963	20.770586699164344
34-35	20.494263323201583	27.14411085918268	31.367428592797314	20.99419722481842
36-37	21.064553185448847	27.644239277457572	30.619792875005235	20.671414662088345
38-39	20.894167544853833	27.737248734872686	30.698788514437496	20.66979520583598
40-41	20.60690500459625	27.521464796483087	30.866633732091824	21.00499646682884
42-43	20.61259493628141	27.527675756719987	30.6648910291702	21.194838277828403
44-45	20.94874730446776	27.76440103966688	30.347909697762702	20.938941958102657
46-47	20.94763241976609	28.140315744067436	29.819603226903418	21.09244860926306
48-49	20.943963353876697	27.90702444479825	29.800501700496284	21.34851050082877
50-51	21.004468439768115	27.81986464609677	29.64825044474283	21.52741646939229
52-53	20.978315872519175	27.961090687424033	29.56057930731558	21.50001413274121
54-55	21.130558937260542	28.42698731483341	29.285893133230896	21.15656061467515
56-57	20.873990463534746	28.147280544047526	29.339607597905104	21.639121394512625
58-59	20.703179766100348	27.697106839705736	29.76222949200759	21.837483902186325
60-61	21.089417370695315	28.144178397003373	29.28666212252913	21.479742109772186
62-63	20.89762802219119	28.137642099923383	29.340240126889693	21.624489750995735
64-65	20.84046639465148	27.851431881807464	29.595834325541748	21.71226739799931
66-67	20.80966135601424	27.988791286169267	29.27345347076786	21.928093887048632
68-69	21.312562284630253	28.08895495895718	29.087597704323603	21.510885052088963
70-71	21.332294766405614	27.846938447525044	29.176351640952003	21.64441514511734
72-73	21.020431620052886	28.04157087957285	29.352542405526442	21.585455094847827
74-75	21.074532470340834	28.02403925464403	29.163930262048236	21.7374980129669
76-77	21.170434336929514	28.108914225838593	28.9285887148365	21.792062722395393
78-79	21.209102815913518	28.239118514909045	28.97035001055605	21.58142865862139
80-81	21.13401133493541	27.944015214638114	29.104061330820574	21.817912119605907
82-83	20.933025095671823	27.999795386490657	29.205026488444464	21.862153029393056
84-85	21.03638750216529	27.95159506044989	29.041025131121206	21.97099230626361
86-87	21.191883101208845	28.049558968058047	28.93881433784675	21.819743592886358
88-89	20.899956588183958	27.887608046457025	29.077799702558394	22.134635662800626
90-91	20.784210627689184	27.774495356986606	29.100111138154254	22.341182877169953
92-93	20.91244257383293	28.01583994164706	28.89493619148009	22.17678129303992
94-95	20.820994982612493	27.758037250437628	29.084262993352674	22.336704773597205
96-97	20.752657079027856	27.73436688990241	29.120833215557777	22.392142815511953
98-99	20.748760295848044	27.794399719040413	29.03158994308767	22.42525004202387
100	20.87673136672569	27.79446898166758	28.891427519416396	22.437372132190333
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	914.0
1	737.0
2	1689.0
3	2478.5
4	1309.5
5	575.0
6	1246.0
7	3232.0
8	5329.5
9	5957.0
10	4390.0
11	2532.5
12	2309.5
13	2639.0
14	3070.0
15	3479.5
16	3944.0
17	4535.0
18	5193.5
19	6096.5
20	7300.0
21	8924.5
22	11392.5
23	15014.5
24	19847.0
25	26228.5
26	34681.5
27	44506.5
28	56102.0
29	68658.0
30	80684.0
31	91365.5
32	99959.5
33	108316.0
34	116437.5
35	122243.5
36	127473.5
37	133528.0
38	137956.5
39	139632.5
40	140553.0
41	140793.5
42	139066.0
43	136993.5
44	134394.0
45	131847.0
46	129606.0
47	126057.5
48	122150.5
49	118914.5
50	115517.5
51	110951.5
52	104562.5
53	98398.0
54	93693.0
55	89034.0
56	83000.5
57	76108.0
58	69492.0
59	62420.0
60	54267.5
61	45072.0
62	36998.5
63	30178.0
64	24237.5
65	19051.0
66	14408.5
67	10730.5
68	7867.0
69	5899.5
70	4306.5
71	3058.0
72	2174.5
73	1508.0
74	1074.0
75	750.0
76	474.0
77	303.5
78	209.5
79	121.5
80	64.5
81	31.0
82	14.5
83	7.5
84	6.5
85	4.0
86	1.5
87	0.5
88	1.5
89	1.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03917126020147435
2	0.0
3	0.0
4	7.504072835531485E-5
5	0.0
6	0.0
7	0.0
8	7.754208596715868E-4
9	0.0
10-11	0.0
12-13	0.042773215162529465
14-15	8.754751641453399E-5
16-17	0.0
18-19	3.7520364177657425E-5
20-21	6.25339402960957E-5
22-23	0.0
24-25	3.7520364177657425E-5
26-27	2.5013576118438284E-5
28-29	2.751493373028211E-4
30-31	1.2506788059219142E-5
32-33	1.250678805921914E-4
34-35	1.2506788059219142E-5
36-37	3.7520364177657425E-5
38-39	2.501357611843828E-4
40-41	1.0005430447375314E-4
42-43	4.0021721789501255E-4
44-45	2.501357611843828E-4
46-47	6.25339402960957E-5
48-49	6.378461910201762E-4
50-51	3.2517648953969767E-4
52-53	5.002715223687656E-4
54-55	2.501357611843828E-4
56-57	4.127240059542317E-4
58-59	8.754751641453399E-5
60-61	1.0005430447375314E-4
62-63	6.503529790793953E-4
64-65	3.2517648953969767E-4
66-67	0.00116313128950738
68-69	0.001325719534277229
70-71	3.1266970148047853E-4
72-73	7.629140716123677E-4
74-75	8.629683760861208E-4
76-77	5.628054626648614E-4
78-79	0.0030266427103310322
80-81	0.0025013576118438283
82-83	0.0012006516536850376
84-85	0.002413810095429294
86-87	0.001150624501448161
88-89	0.0017384435402314608
90-91	0.002551384764080705
92-93	0.0021761811223041305
94-95	0.003276778471515415
96-97	0.0028015205252650876
98-99	0.0025263711879622668
100	0.0012006516536850376
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3997829.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.97315964113021
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.9615344136748	53.55276609436769
2	12.755897935963754	17.086055776625276
3	3.4366102839894266	6.9048194752192105
4	1.3885942524356183	3.719941781805061
5	0.7103840647764453	2.3788332688393927
6	0.416601890158339	1.674068693782262
7	0.27504175294269134	1.2894290659465015
8	0.1802501875353232	0.9657539668117489
9	0.14839224409870586	0.894446770817537
>10	0.6899717001439841	7.90422378680351
>50	0.02669993519717429	1.2038754178167232
>100	0.009160737574376902	1.005805782354733
>500	4.8642696321075566E-4	0.2361371166661179
>1k	2.6192218237068446E-4	0.3672241348282718
>5k	3.741745462438143E-5	0.18466457278213852
>10k+	7.483490924876287E-5	0.6319542945338539
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12895	0.32255006404726166	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12323	0.30824229850751494	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7369	0.1843250424167717	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.501357611843828E-5	0.0	2.501357611843828E-5	0.0
3	0.0	2.501357611843828E-5	0.0	2.501357611843828E-5	0.0
4	0.0	2.501357611843828E-5	0.0	2.501357611843828E-5	0.0
5	0.0	2.501357611843828E-5	0.0	2.501357611843828E-5	0.0
6	0.0	2.501357611843828E-5	0.0	2.501357611843828E-5	0.0
7	0.0	2.501357611843828E-5	0.0	5.002715223687656E-5	0.0
8	0.0	2.501357611843828E-5	0.0	5.002715223687656E-5	0.0
9	0.0	2.501357611843828E-5	0.0	7.504072835531485E-5	0.0
10-11	0.0	2.501357611843828E-5	0.0	7.504072835531485E-5	0.0
12-13	0.0	2.501357611843828E-5	0.0	7.504072835531485E-5	0.0
14-15	0.0	3.7520364177657425E-5	0.0	7.504072835531485E-5	0.0
16-17	0.0	5.002715223687656E-5	0.0	1.3757466865141056E-4	0.0
18-19	0.0	7.504072835531485E-5	0.0	1.7509503282906797E-4	0.0
20-21	0.0	7.504072835531485E-5	0.0	2.3762897312516368E-4	0.0
22-23	0.0	7.504072835531485E-5	0.0	5.127783104279847E-4	0.0
24-25	0.0	8.754751641453399E-5	0.0	0.0011381177133889418	0.0
26-27	0.0	1.0005430447375312E-4	0.0	0.0024138100954292944	0.0
28-29	0.0	1.0005430447375312E-4	0.0	0.006065792208721284	0.0
30-31	0.0	1.2506788059219142E-4	0.0	0.016221304112807228	0.0
32-33	0.0	1.2506788059219142E-4	0.0	0.040396925431277826	0.0
34-35	0.0	1.2506788059219142E-4	0.0	0.07740451129850726	0.0
36-37	0.0	1.500814567106297E-4	0.0	0.13503579067538907	0.0
38-39	0.0	1.7509503282906797E-4	0.0	0.20709990347260976	0.0
40-41	0.0	1.7509503282906797E-4	0.0	0.28979478612016674	0.0
42-43	0.0	1.7509503282906797E-4	0.0	0.37652936131085146	0.0
44-45	0.0	1.7509503282906797E-4	0.0	0.4755831227398671	0.0
46-47	0.0	2.0010860894750625E-4	0.0	0.5895449755354718	0.0
48-49	0.0	2.0010860894750625E-4	0.0	0.7091849101099622	0.0
50-51	0.0	2.0010860894750625E-4	0.0	0.8313762294485332	0.0
52-53	0.0	2.0010860894750625E-4	0.0	0.9493777747872658	0.0
54-55	0.0	2.0010860894750625E-4	0.0	1.081962235003048	0.0
56-57	0.0	2.0010860894750625E-4	0.0	1.2348702258150612	0.0
58-59	0.0	2.0010860894750625E-4	0.0	1.3938940359880325	0.0
60-61	0.0	2.0010860894750625E-4	0.0	1.5538683620535045	0.0
62-63	0.0	2.0010860894750625E-4	0.0	1.7128296382861798	0.0
64-65	0.0	2.0010860894750625E-4	0.0	1.8882248340286691	0.0
66-67	0.0	2.0010860894750625E-4	0.0	2.088658619465715	0.0
68-69	0.0	2.0010860894750625E-4	0.0	2.297071735684543	0.0
70-71	0.0	2.0010860894750625E-4	0.0	2.516103114965648	0.0
72-73	0.0	2.2512218506594455E-4	0.0	2.7510931558103158	0.0
74-75	0.0	2.5013576118438285E-4	0.0	2.9847699839087665	0.0
76-77	0.0	2.751493373028211E-4	0.0	3.2315789394693972	0.0
78-79	0.0	2.751493373028211E-4	0.0	3.505002340020046	0.0
80-81	0.0	2.751493373028211E-4	0.0	3.7985616693460376	0.0
82-83	0.0	2.751493373028211E-4	0.0	4.099212347501606	0.0
84-85	0.0	2.751493373028211E-4	0.0	4.406178953627081	0.0
86-87	0.0	2.751493373028211E-4	0.0	4.723826356755129	0.0
88	0.0	4.002172178950125E-4	0.0	4.979827801539286	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7210	0.0	59.333607	1
GTATCAA	13200	0.0	40.172585	1
TCAACGC	16315	0.0	32.089455	4
ATCAACG	16320	0.0	32.079624	3
GTGGTAT	2925	0.0	31.179596	1
TATCAAC	16850	0.0	31.154264	2
CAACGCA	16875	0.0	31.108109	5
AACGCAG	17560	0.0	29.968376	6
TGGTATC	3095	0.0	29.0026	2
ACGCAGA	19915	0.0	26.235624	7
CGCAGAG	20045	0.0	25.97163	8
GTACATG	9505	0.0	24.383125	1
TATAACG	260	2.910383E-11	23.49818	2
GCAGAGT	21865	0.0	23.42265	9
TACATGG	9750	0.0	22.654657	2
ACATGGG	9795	0.0	21.974817	3
CAGAGTA	21210	0.0	21.507439	10-11
GAGTACT	16845	0.0	21.112946	12-13
AGAGTAC	20300	0.0	21.058403	10-11
AGTACTT	17525	0.0	19.15363	12-13
>>END_MODULE