FastQCFastQC Report
Wed 25 May 2016
SRR2049363_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049363_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5515162
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56400.045.7604061
GTATCAA91750.033.9194341
TCAACGC94800.031.6371364
ATCAACG96800.031.1777293
CAACGCA101300.029.8391425
AACGCAG106000.028.5127226
TATCAAC110800.027.5352942
ACGCAGA124900.023.7082717
CGCAGAG126300.022.9616788
GTACATG147850.022.8932691
TACATGG147300.021.6058182
ACATGGG152750.019.911683
GCAGAGT147050.018.6348259
GTGGTAT30700.018.2223831
CAGAGTA140100.017.59661710-11
GAGTACA116050.016.7302151
CATGGGG114150.016.6787664
AGAGTAC128850.016.12351210-11
GTATTAG26500.015.9658951
TATACTG33650.015.6465215
Produced by FastQC (version 0.11.5)