##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049363_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5515162
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.32244782655523	31.0	31.0	33.0	27.0	34.0
2	30.504396969662903	31.0	31.0	34.0	27.0	34.0
3	30.561355224016992	31.0	31.0	34.0	27.0	34.0
4	34.277559027277896	37.0	35.0	37.0	30.0	37.0
5	34.27789700465734	37.0	35.0	37.0	30.0	37.0
6	34.4305857198755	37.0	35.0	37.0	31.0	37.0
7	34.408141048259324	37.0	35.0	37.0	30.0	37.0
8	34.36226623986748	37.0	35.0	37.0	30.0	37.0
9	35.94690708994586	39.0	37.0	39.0	30.0	39.0
10-11	35.88913816130877	39.0	37.0	39.0	30.0	39.0
12-13	35.82251174126889	39.0	37.0	39.0	30.0	39.0
14-15	36.978408884453444	40.0	37.0	41.0	31.0	41.0
16-17	36.938732534057934	40.0	37.0	41.0	31.0	41.0
18-19	36.956849408956614	40.0	37.0	41.0	31.0	41.0
20-21	37.02210397446167	40.0	37.0	41.0	31.0	41.0
22-23	36.974654778953	40.0	37.0	41.0	31.0	41.0
24-25	36.971365483008476	40.0	37.0	41.0	30.5	41.0
26-27	36.94587964959143	40.0	37.0	41.0	30.0	41.0
28-29	36.88625003218401	40.0	37.0	41.0	30.0	41.0
30-31	36.81959686768947	40.0	37.0	41.0	30.0	41.0
32-33	36.76051075199604	40.0	37.0	41.0	30.0	41.0
34-35	36.663221588051265	40.0	37.0	41.0	30.0	41.0
36-37	36.57744514123067	40.0	36.5	41.0	30.0	41.0
38-39	36.464564141542894	40.0	36.0	41.0	30.0	41.0
40-41	36.35428968360313	40.0	36.0	41.0	30.0	41.0
42-43	36.21432561727108	40.0	36.0	41.0	29.0	41.0
44-45	36.082221066217095	40.0	35.5	41.0	29.0	41.0
46-47	35.95717387811999	39.0	35.0	41.0	28.5	41.0
48-49	35.8252069476835	39.0	35.0	41.0	28.0	41.0
50-51	35.68373712322503	39.0	35.0	41.0	28.0	41.0
52-53	35.525420468156696	39.0	35.0	41.0	27.5	41.0
54-55	35.3779389435886	39.0	35.0	40.5	27.0	41.0
56-57	35.24060426511497	39.0	35.0	40.0	27.0	41.0
58-59	35.03945033708892	38.5	34.0	40.0	26.5	41.0
60-61	34.810655879192666	38.0	34.0	40.0	26.0	41.0
62-63	34.58935077519028	38.0	34.0	40.0	26.0	41.0
64-65	34.336991551653426	37.5	34.0	40.0	26.0	41.0
66-67	34.070016075683725	37.0	34.0	40.0	25.5	41.0
68-69	33.77374952902562	36.5	33.0	39.5	25.0	41.0
70-71	33.47827797261441	36.0	33.0	39.0	24.5	41.0
72-73	33.132648234086325	36.0	33.0	39.0	23.5	40.5
74-75	32.78321742860862	35.0	33.0	38.5	23.0	40.0
76-77	32.45309149577111	35.0	32.0	37.5	22.0	39.5
78-79	32.129991195181574	35.0	32.0	37.0	22.0	39.0
80-81	31.776900388420138	35.0	32.0	36.5	20.5	39.0
82-83	31.460926714392073	35.0	32.0	36.0	20.0	38.5
84-85	31.16740070373273	35.0	32.0	36.0	19.5	37.0
86-87	30.84421663407167	35.0	31.5	35.0	17.5	37.0
88-89	30.588951784190563	35.0	31.0	35.0	15.0	36.5
90-91	30.374182662268126	35.0	31.0	35.0	9.0	36.0
92-93	30.123700083515224	34.0	31.0	35.0	4.5	36.0
94-95	29.903767015366004	34.0	31.0	35.0	2.0	36.0
96-97	29.624116843711935	34.0	31.0	35.0	2.0	35.0
98-99	29.397393857152338	34.0	31.0	35.0	2.0	35.0
100	29.255417338602204	34.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3759132055110541
1101	2	0.41050719381208367
1101	3	0.36803486256740925
1101	4	0.4169400081236603
1101	5	0.45889743803454763
1101	6	0.533193812697732
1101	7	0.5453254578104207
1101	8	0.5907260542032802
1101	9	0.7607905566016129
1101	10-11	0.7768624864383753
1101	12-13	0.8118190628304802
1101	14-15	0.904594253432478
1101	16-17	0.9287012773923706
1101	18-19	0.9121486776221559
1101	20-21	0.8439160957747731
1101	22-23	0.8447106384315504
1101	24-25	0.9015224830087405
1101	26-27	0.8027416655759154
1101	28-29	0.7525658911898887
1101	30-31	0.81118831000407
1101	32-33	0.8240697293123134
1101	34-35	0.8293861057264991
1101	36-37	0.7901731577859508
1101	38-39	0.848978617682981
1101	40-41	0.8406494100758621
1101	42-43	0.7894524844005204
1101	44-45	0.7831381856782471
1101	46-47	0.7059546962543735
1101	48-49	0.6822861725825575
1101	50-51	0.6881364414723876
1101	52-53	0.7577934591613626
1101	54-55	0.7684774777065471
1101	56-57	0.7712838078226838
1101	58-59	0.8198542860365521
1101	60-61	0.7727185314175458
1101	62-63	0.7202563050069415
1101	64-65	0.7430308956680847
1101	66-67	0.7766107742622168
1101	68-69	0.6951389667099548
1101	70-71	0.7802123670378833
1101	72-73	0.8485501084134484
1101	74-75	0.762462075905276
1101	76-77	0.7845499298967553
1101	78-79	0.7945997455209692
1101	80-81	0.7768132968848498
1101	82-83	0.7764693168302621
1101	84-85	0.743817384908926
1101	86-87	0.728893135542787
1101	88-89	0.804002018254419
1101	90-91	0.7890383992671168
1101	92-93	0.7156801609913508
1101	94-95	0.7087164056056601
1101	96-97	0.7236444448243802
1101	98-99	0.851376660754287
1101	100	0.9086734105401675
1102	1	0.31452004753904816
1102	2	0.31587979772876906
1102	3	0.3388295700420727
1102	4	0.38646735783376585
1102	5	0.32153661947931056
1102	6	0.5424337531850796
1102	7	0.6093531423565892
1102	8	0.6154178325588191
1102	9	0.6548787050068512
1102	10-11	0.6434867706379492
1102	12-13	0.6782233351516425
1102	14-15	0.8260847217488987
1102	16-17	0.7853328821322094
1102	18-19	0.8234341526230224
1102	20-21	0.7611290481596384
1102	22-23	0.7808365394073178
1102	24-25	0.8173810460992001
1102	26-27	0.7860007399809987
1102	28-29	0.7811717989252358
1102	30-31	0.8325613790887232
1102	32-33	0.8722745392793243
1102	34-35	0.8523516683313375
1102	36-37	0.791601631573279
1102	38-39	0.8205535456717712
1102	40-41	0.8379272616571498
1102	42-43	0.8357277949802295
1102	44-45	0.7286777631348187
1102	46-47	0.6622911532245652
1102	48-49	0.6733202418128172
1102	50-51	0.7733405086320673
1102	52-53	0.7493492548916763
1102	54-55	0.6659942695268413
1102	56-57	0.6848356684195807
1102	58-59	0.8199230417428609
1102	60-61	0.8157955090586881
1102	62-63	0.7457671130785073
1102	64-65	0.6984597438106519
1102	66-67	0.8032036770140607
1102	68-69	0.7884838243686474
1102	70-71	0.8253311793553664
1102	72-73	0.7251764293582212
1102	74-75	0.722552798656892
1102	76-77	0.7754109172660364
1102	78-79	0.7581541396846703
1102	80-81	0.7886503108727858
1102	82-83	0.8057764785248231
1102	84-85	0.7718193049951907
1102	86-87	0.831801872860634
1102	88-89	0.7996385237867685
1102	90-91	0.8334160236346975
1102	92-93	0.8116856865920674
1102	94-95	0.8092219877968176
1102	96-97	0.8113271476181367
1102	98-99	0.8802668209647351
1102	100	0.8533141142310079
1103	1	0.10363514227735848
1103	2	0.18747580025128485
1103	3	0.18715337799118714
1103	4	0.29232868802063194
1103	5	0.22742803831766167
1103	6	0.38468036884339085
1103	7	0.3578849318311228
1103	8	0.42156689365591404
1103	9	0.467546456468078
1103	10-11	0.455211173627049
1103	12-13	0.48508721859724346
1103	14-15	0.5058770388270943
1103	16-17	0.4548202393891856
1103	18-19	0.5166841631700265
1103	20-21	0.4822208321527697
1103	22-23	0.5017831079891408
1103	24-25	0.5103518729219445
1103	26-27	0.4878895798094689
1103	28-29	0.46236297956860994
1103	30-31	0.5332736795912183
1103	32-33	0.5488623388775267
1103	34-35	0.5121814435738301
1103	36-37	0.492596448837304
1103	38-39	0.5332345851459479
1103	40-41	0.528827283079913
1103	42-43	0.5672937398987301
1103	44-45	0.44695798561003386
1103	46-47	0.500003848943102
1103	48-49	0.5705227673236379
1103	50-51	0.5393262271609416
1103	52-53	0.5763758906991825
1103	54-55	0.5192162420773414
1103	56-57	0.46897774219214483
1103	58-59	0.5446354478067619
1103	60-61	0.4958232998929759
1103	62-63	0.5016810474206537
1103	64-65	0.5501296624461887
1103	66-67	0.5847687346445625
1103	68-69	0.6257005671858309
1103	70-71	0.541245424780989
1103	72-73	0.5241859952664996
1103	74-75	0.5643303996602498
1103	76-77	0.5831408044559652
1103	78-79	0.5276567908647039
1103	80-81	0.5059490908472206
1103	82-83	0.5056175398272025
1103	84-85	0.545368986335518
1103	86-87	0.5491411667401032
1103	88-89	0.4915587666096428
1103	90-91	0.5406628958912201
1103	92-93	0.45119989960657136
1103	94-95	0.5452607545815518
1103	96-97	0.48946758707620575
1103	98-99	0.4775177333494405
1103	100	0.4080460713740557
1104	1	0.08822606044136094
1104	2	0.08114773635055172
1104	3	0.07681556602190653
1104	4	0.22219143299034982
1104	5	0.22869399468532237
1104	6	0.27462274430927636
1104	7	0.3429924583609889
1104	8	0.34545367011788386
1104	9	0.4528617716359946
1104	10-11	0.411429739460047
1104	12-13	0.35090770557527406
1104	14-15	0.45616462975507943
1104	16-17	0.4607307626565955
1104	18-19	0.3952771068642136
1104	20-21	0.40484533354184293
1104	22-23	0.4410630140097709
1104	24-25	0.5011347288867896
1104	26-27	0.4422709750365499
1104	28-29	0.47018061804255495
1104	30-31	0.4435658435053682
1104	32-33	0.4255990919428214
1104	34-35	0.5002325743039648
1104	36-37	0.4132164852248579
1104	38-39	0.5303909894535508
1104	40-41	0.5254507177115713
1104	42-43	0.533869219530537
1104	44-45	0.5205262073646892
1104	46-47	0.4033821322489999
1104	48-49	0.4138339774388555
1104	50-51	0.44573319347954765
1104	52-53	0.4172607185291213
1104	54-55	0.48410037127933236
1104	56-57	0.4656310362147025
1104	58-59	0.5036961777175364
1104	60-61	0.4124241804586859
1104	62-63	0.4281942231836595
1104	64-65	0.43918300522545195
1104	66-67	0.537380245153436
1104	68-69	0.49219923046680236
1104	70-71	0.45261641257872753
1104	72-73	0.5330069910812725
1104	74-75	0.5474130095509935
1104	76-77	0.5714389909683533
1104	78-79	0.504630905636013
1104	80-81	0.5415686854794011
1104	82-83	0.4422348471482316
1104	84-85	0.5055263330545863
1104	86-87	0.5613571343970634
1104	88-89	0.49761408747129465
1104	90-91	0.4969927186327858
1104	92-93	0.5036783926644688
1104	94-95	0.6231630873771437
1104	96-97	0.515025180523832
1104	98-99	0.4521493175496545
1104	100	0.44504717412635486
1105	1	0.042303455656156785
1105	2	0.08287566458594142
1105	3	0.06281201074675735
1105	4	0.108709197016303
1105	5	0.08176219443352295
1105	6	0.19114071529300958
1105	7	0.15550533683008183
1105	8	0.27025498501591727
1105	9	0.3631115865864203
1105	10-11	0.2830114751489248
1105	12-13	0.30712464660854977
1105	14-15	0.30846354010829913
1105	16-17	0.34185434430504813
1105	18-19	0.28800553552011365
1105	20-21	0.31404819310052545
1105	22-23	0.3560627777282832
1105	24-25	0.2910977251751987
1105	26-27	0.2827342480393398
1105	28-29	0.31934696590778344
1105	30-31	0.2580996759133356
1105	32-33	0.28010821404372876
1105	34-35	0.3362493637674646
1105	36-37	0.26079020279437515
1105	38-39	0.30271850481100415
1105	40-41	0.33373242344513443
1105	42-43	0.2955882457620689
1105	44-45	0.2730380375108936
1105	46-47	0.2004089723601865
1105	48-49	0.22278414409343839
1105	50-51	0.24851190187807504
1105	52-53	0.27416343839412605
1105	54-55	0.3280966869798263
1105	56-57	0.33339856691974035
1105	58-59	0.2937616942328205
1105	60-61	0.2962601989046547
1105	62-63	0.2662568987946585
1105	64-65	0.26403646699742467
1105	66-67	0.2730414267435961
1105	68-69	0.26317872890265903
1105	70-71	0.2538909270653775
1105	72-73	0.1804725746238134
1105	74-75	0.2166371578464492
1105	76-77	0.28907450996375417
1105	78-79	0.2485841507169937
1105	80-81	0.2744627443589387
1105	82-83	0.23398104264968467
1105	84-85	0.18117125464353023
1105	86-87	0.2113100881581822
1105	88-89	0.2721308861830387
1105	90-91	0.3279597384171602
1105	92-93	0.3110563216949842
1105	94-95	0.2965316708261838
1105	96-97	0.3109310369551359
1105	98-99	0.34419291757505377
1105	100	0.3829901525791719
1106	1	0.17239837066327013
1106	2	0.1423436352594969
1106	3	0.16665410990443164
1106	4	0.3117649727429068
1106	5	0.19792478838652272
1106	6	0.34684350658065455
1106	7	0.29626828837992036
1106	8	0.3145939253517156
1106	9	0.4420589667968784
1106	10-11	0.39049188901422127
1106	12-13	0.382749597693703
1106	14-15	0.4726440346741967
1106	16-17	0.5130865193595682
1106	18-19	0.4734932141361128
1106	20-21	0.4479904678391904
1106	22-23	0.4914344623873603
1106	24-25	0.47513299667816966
1106	26-27	0.38870996129340085
1106	28-29	0.4663846620941001
1106	30-31	0.4076721405899377
1106	32-33	0.43305454607635596
1106	34-35	0.46254429135922237
1106	36-37	0.40786584299094386
1106	38-39	0.44859883401839795
1106	40-41	0.4609839541533418
1106	42-43	0.4550043777225099
1106	44-45	0.40484343999128924
1106	46-47	0.40478618387998466
1106	48-49	0.4359080676697147
1106	50-51	0.4568268735459853
1106	52-53	0.4572077467484661
1106	54-55	0.46613393496524935
1106	56-57	0.3745157146053657
1106	58-59	0.444097288300604
1106	60-61	0.44030873519918856
1106	62-63	0.4065955290620522
1106	64-65	0.4772583933679826
1106	66-67	0.482579050809143
1106	68-69	0.44655366602183477
1106	70-71	0.42653316670413943
1106	72-73	0.48133684957792156
1106	74-75	0.45105230047960987
1106	76-77	0.5472946182121063
1106	78-79	0.38766297196561794
1106	80-81	0.4947309413118397
1106	82-83	0.4890489539432359
1106	84-85	0.4535120803689985
1106	86-87	0.47720665658742334
1106	88-89	0.47186896390689625
1106	90-91	0.4563421431754868
1106	92-93	0.44221459892288095
1106	94-95	0.45267182283165397
1106	96-97	0.4826305003019371
1106	98-99	0.5315975764614542
1106	100	0.4989807427361441
1107	1	0.04286557719960271
1107	2	0.007837191734772375
1107	3	0.060697087431943686
1107	4	0.08419187286413887
1107	5	0.09160680621069872
1107	6	0.1987814925679956
1107	7	0.11340684295043246
1107	8	0.22819169050064403
1107	9	0.24460879526871082
1107	10-11	0.19687312006485058
1107	12-13	0.12704609807077105
1107	14-15	0.19375071154647117
1107	16-17	0.26352946379999054
1107	18-19	0.21473431957653588
1107	20-21	0.1528849689150178
1107	22-23	0.15920186732019204
1107	24-25	0.20459268412723475
1107	26-27	0.21706812995356728
1107	28-29	0.27715411798860856
1107	30-31	0.21987478215747558
1107	32-33	0.2836251822671656
1107	34-35	0.25853186499507785
1107	36-37	0.24412250592990148
1107	38-39	0.2864596303757949
1107	40-41	0.3368317660175606
1107	42-43	0.30537313491429074
1107	44-45	0.2374140765801016
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2216	2	-0.9719032859548378
2216	3	-1.029577687610761
2216	4	-1.2578695848853272
2216	5	-1.218121855824954
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2216	82-83	-3.9003421502736195
2216	84-85	-3.8904461786987348
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2216	90-91	-3.725053625049984
2216	92-93	-3.7478213498634894
2216	94-95	-3.6585143279976613
2216	96-97	-3.920444870893462
2216	98-99	-3.6744628280193012
2216	100	-3.3113977666979153
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	99481.0
3	15863.0
4	11089.0
5	9097.0
6	8373.0
7	8842.0
8	9964.0
9	11402.0
10	12785.0
11	14328.0
12	15804.0
13	17395.0
14	19141.0
15	20153.0
16	20452.0
17	20867.0
18	19888.0
19	18888.0
20	19075.0
21	19787.0
22	22007.0
23	25873.0
24	32507.0
25	40108.0
26	48724.0
27	57611.0
28	69914.0
29	86586.0
30	104415.0
31	124320.0
32	157627.0
33	207412.0
34	284142.0
35	409401.0
36	662972.0
37	1094712.0
38	1293568.0
39	399911.0
40	678.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.80467563329015	20.464672702768183	14.520718210002364	23.209933453939303
2	15.413623638175789	20.072515750591528	40.73970418724953	23.77415642398316
3	18.361287661527683	25.095030688967736	30.253487118868307	26.290194530636274
4	12.690319697186261	16.90724551667195	38.723678110214735	31.67875667592705
5	13.320444866159411	37.70923277039706	35.56955736314151	13.400765000302018
6	31.134200139308106	37.68059263988855	17.704735749941953	13.480471470861389
7	27.083879004927187	32.38726273715402	22.608688712161833	17.920169545756966
8	24.681690378946026	36.55147632723254	20.566733059960562	18.20010023386087
9	24.752117683336053	17.071647530427526	21.679146033991763	36.49708875224466
10-11	23.81513557399333	27.007680645911414	29.030307570471525	20.146876209623734
12-13	24.19462721123072	24.55685144706945	28.98233754394029	22.26618379775954
14-15	21.693014972793435	26.78235718401237	26.089851997048118	25.434775846146074
16-17	21.321986545754946	30.04490885051426	26.841587995237315	21.791516608493477
18-19	21.25807379895963	29.08387826762183	28.699622618853386	20.958425314565158
20-21	22.298823867426883	28.291464377740944	28.30028872765027	21.1094230271819
22-23	22.00336398332193	28.10439739333918	28.301273673810428	21.59096494952846
24-25	21.869932103679513	28.23578576664002	28.238606239975304	21.655675889705165
26-27	21.593831173483462	28.22714618133089	28.288298648224856	21.890723996960787
28-29	21.61890579569508	28.246935099750964	28.446754443402504	21.68740466115145
30-31	21.920609951802238	28.506322754069874	28.069926435945586	21.503140858182306
32-33	21.6017598375981	28.319311924974734	28.382251367436474	21.696676869990693
34-35	21.81430553279471	28.064028359344483	28.37390941032998	21.74775669753083
36-37	21.997716460168647	28.349312670834713	27.93615338712983	21.71681748186681
38-39	21.872810711528444	28.498492613247173	28.060229085785327	21.568467589439052
40-41	21.788398740896884	28.218561713474323	28.203443697270025	21.78959584835877
42-43	21.665475304522865	28.209272963165233	28.197755354010496	21.927496378301406
44-45	21.77300940143493	28.28164419866976	28.128089937162642	21.817256462732665
46-47	21.796949945124695	28.333921898411674	28.0197968262467	21.84933133021693
48-49	21.745329158540265	28.261681025906487	28.041608392052318	21.951381423500933
50-51	21.779657865883355	28.170989065601855	28.0017448604157	22.04760820809909
52-53	21.762434858913995	28.15736394113784	28.061940519884427	22.018260680063733
54-55	21.835483130536165	28.413498908422184	27.914020324668336	21.836997636373315
56-57	21.720738211162693	28.24817409687897	27.920773905782166	22.11031378617617
58-59	21.66362619336612	27.90958603372586	28.19720368353733	22.22958408937069
60-61	21.818543744833633	28.22067418196229	27.932205449518875	22.028576623685197
62-63	21.67923648627631	28.261158781293844	27.91551934925145	22.144085383178396
64-65	21.731111430728717	28.054216778969128	27.99847212304706	22.216199667255097
66-67	21.626763060454394	28.069065084850898	27.901775552530655	22.402396302164053
68-69	21.90523745230749	28.135689779382904	27.861496371415033	22.09757639689457
70-71	21.858642114587816	27.96412592166425	27.982952793523264	22.194279170224668
72-73	21.688675974603242	28.086790361579954	28.006397343557136	22.218136320259667
74-75	21.723267436768325	28.091319973856965	27.951109949923953	22.234302639450753
76-77	21.762795474995432	28.07183628843848	27.82329875669567	22.342069479870418
78-79	21.799255020864457	28.071470041496756	27.93961958072297	22.189655356915814
80-81	21.75102664930668	27.94944649402991	27.993584048305188	22.30594280835822
82-83	21.65208629442591	28.00371885870722	28.04852689829065	22.295667948576224
84-85	21.709726957845614	27.876622548663082	27.977194402582327	22.436456090908976
86-87	21.83630990653209	27.95020280430939	27.927231677193358	22.286255611965156
88-89	21.70560548277297	27.8255244549453	28.013085176164427	22.455784886117307
90-91	21.69546374781759	27.70889718624023	28.039999230965396	22.55563983497678
92-93	21.73480545942243	27.929807270439326	27.87569441207427	22.459692858063978
94-95	21.64986972660271	27.703406515574315	27.990987420659287	22.655736337163695
96-97	21.72575347741372	27.639423828348107	28.025242050913462	22.609580643324712
98-99	21.72102106882808	27.695986083455026	27.955579545986136	22.627413301730755
100	21.722027385596288	27.605009608058655	27.990220414196354	22.6827425921487
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1181.0
1	976.0
2	954.0
3	1143.5
4	1111.0
5	929.0
6	732.0
7	672.5
8	671.0
9	667.0
10	618.5
11	572.0
12	604.5
13	678.5
14	752.0
15	869.5
16	1074.5
17	1396.0
18	1873.5
19	2648.5
20	3779.0
21	5521.0
22	8298.0
23	12496.5
24	18178.5
25	25815.0
26	36042.5
27	48637.0
28	63561.5
29	80446.0
30	97147.0
31	111644.5
32	124378.0
33	136917.0
34	149849.5
35	160870.0
36	170412.5
37	180084.0
38	189357.5
39	195021.5
40	198368.0
41	201322.0
42	201746.5
43	200825.5
44	197754.5
45	194189.0
46	190890.0
47	184454.5
48	177687.5
49	173007.5
50	168216.0
51	161897.5
52	152403.5
53	143337.0
54	136291.5
55	129377.0
56	119724.0
57	108000.5
58	98226.0
59	90069.0
60	80023.5
61	68520.0
62	59024.0
63	50045.5
64	41438.0
65	33675.5
66	27036.5
67	21412.0
68	16613.5
69	13085.5
70	10042.0
71	7456.5
72	5690.0
73	4290.0
74	3183.0
75	2288.0
76	1567.0
77	1122.0
78	802.5
79	585.5
80	382.0
81	228.5
82	171.5
83	127.0
84	92.5
85	74.0
86	59.0
87	45.5
88	44.5
89	40.0
90	30.0
91	21.0
92	18.5
93	19.5
94	13.0
95	12.0
96	15.0
97	14.0
98	14.5
99	16.0
100	27.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014251621257906839
2	0.034033451782558705
3	0.035230152804215
4	0.0375328956792203
5	0.04030706622942354
6	0.0399262977225329
7	0.03767795034851197
8	0.03726091817429841
9	0.03738784100992863
10-11	0.03778674135048073
12-13	0.03881118995235317
14-15	0.03739690692675936
16-17	0.034151308701358185
18-19	0.036907347417899965
20-21	0.03640872199220984
22-23	0.03726091817429841
24-25	0.03450487945775664
26-27	0.03432356112114204
28-29	0.03503070263393895
30-31	0.03516669138639989
32-33	0.03492191163197019
34-35	0.03476779104584779
36-37	0.03249224592133468
38-39	0.03286394851139459
40-41	0.03395185853108213
42-43	0.033815869778621194
44-45	0.032664498341118536
46-47	0.031785104408537775
48-49	0.03182136807586069
50-51	0.03312686009948575
52-53	0.03337163985391545
54-55	0.03286394851139459
56-57	0.031295544899678375
58-59	0.03202081824613674
60-61	0.031540324654108075
62-63	0.031866697660014334
64-65	0.029536757034516845
66-67	0.03022576671365229
68-69	0.031150490230386702
70-71	0.03202081824613674
72-73	0.02943703194937882
74-75	0.02987219595725384
76-77	0.030987303727433576
78-79	0.031168622064048163
80-81	0.031123292479894517
82-83	0.029781536788946548
84-85	0.03066093072152731
86-87	0.031168622064048163
88-89	0.03168537932339975
90-91	0.031839499909522145
92-93	0.030552139719558558
94-95	0.03185763174318361
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5515162.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.5262429241225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.31376814436716	49.11453216518352
2	13.606235678544994	17.287060659962243
3	4.18466660123994	7.975084412004914
4	1.772204127102127	4.503258795576887
5	0.9093013442338004	2.888224904251376
6	0.5421020202044892	2.0662622775100763
7	0.34743763904703745	1.5449985501359982
8	0.24097323760194056	1.2246499544090532
9	0.18998846347337578	1.08623279550514
>10	0.8401684267597939	9.216059355410376
>50	0.03930593556424638	1.6744422461126511
>100	0.0137626494802794	1.3587041403512972
>500	5.715493049118826E-5	0.02793929794193905
>1k	2.8577450308451505E-5	0.032550445644509866
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.8131833661459082E-5	0.0
3	0.0	0.0	0.0	1.8131833661459082E-5	0.0
4	0.0	0.0	0.0	9.065916830729542E-5	0.0
5	0.0	0.0	0.0	9.065916830729542E-5	0.0
6	0.0	0.0	0.0	1.269228356302136E-4	0.0
7	0.0	0.0	0.0	1.6318650295313176E-4	0.0
8	0.0	1.8131833661459082E-5	0.0	1.6318650295313176E-4	0.0
9	0.0	1.8131833661459082E-5	0.0	2.17582003937509E-4	0.0
10-11	0.0	1.8131833661459082E-5	0.0	2.2664792076823852E-4	0.0
12-13	0.0	1.8131833661459082E-5	0.0	2.3571383759896807E-4	0.0
14-15	0.0	1.8131833661459082E-5	0.0	2.629115880911567E-4	0.0
16-17	0.0	1.8131833661459082E-5	0.0	3.4450483956772256E-4	0.0
18-19	0.0	1.8131833661459082E-5	0.0	3.717025900599112E-4	0.0
20-21	0.0	1.8131833661459082E-5	0.0	4.623617583672066E-4	0.0
22-23	0.0	1.8131833661459082E-5	0.0	6.074164276588793E-4	0.0
24-25	0.0	1.8131833661459082E-5	0.0	9.791190177187905E-4	0.0
26-27	0.0	1.8131833661459082E-5	0.0	0.0019219743681146628	0.0
28-29	0.0	1.8131833661459082E-5	0.0	0.005231034011330946	0.0
30-31	1.8131833661459082E-5	1.8131833661459082E-5	0.0	0.01599227728940691	0.0
32-33	3.6263667322918165E-5	1.8131833661459082E-5	0.0	0.039663386134441744	0.0
34-35	3.6263667322918165E-5	1.8131833661459082E-5	0.0	0.07679738147310994	0.0
36-37	3.6263667322918165E-5	2.7197750492188622E-5	0.0	0.136161004880727	0.0
38-39	3.6263667322918165E-5	3.6263667322918165E-5	0.0	0.21460838321702969	0.0
40-41	3.6263667322918165E-5	3.6263667322918165E-5	0.0	0.29511372467390806	0.0
42-43	3.6263667322918165E-5	3.6263667322918165E-5	0.0	0.37405066251907015	0.0
44-45	3.6263667322918165E-5	3.6263667322918165E-5	0.0	0.4724430578829779	0.0
46-47	3.6263667322918165E-5	3.6263667322918165E-5	0.0	0.586474159779894	0.0
48-49	3.6263667322918165E-5	3.6263667322918165E-5	0.0	0.7034516846467973	0.0
50-51	3.6263667322918165E-5	5.439550098437725E-5	0.0	0.8190874538227526	0.0
52-53	3.6263667322918165E-5	5.439550098437725E-5	0.0	0.9301449349991895	0.0
54-55	3.6263667322918165E-5	6.34614178151068E-5	0.0	1.0529427784714211	0.0
56-57	3.6263667322918165E-5	7.252733464583633E-5	0.0	1.2007172228123126	0.0
58-59	3.6263667322918165E-5	7.252733464583633E-5	0.0	1.3566056627167071	0.0
60-61	3.6263667322918165E-5	7.252733464583633E-5	0.0	1.5167278857810524	0.0
62-63	3.6263667322918165E-5	8.159325147656587E-5	0.0	1.6759344512454937	0.0
64-65	3.6263667322918165E-5	9.065916830729542E-5	0.0	1.843871494617928	0.0
66-67	3.6263667322918165E-5	9.972508513802496E-5	0.0	2.0327236081188547	0.0
68-69	3.6263667322918165E-5	1.087910019687545E-4	0.0	2.2396712916139183	0.0
70-71	5.439550098437725E-5	1.087910019687545E-4	0.0	2.456346703868354	0.0
72-73	5.439550098437725E-5	1.087910019687545E-4	0.0	2.6774734812866785	0.0
74-75	5.439550098437725E-5	1.1785691879948404E-4	0.0	2.8975486123526384	0.0
76-77	5.439550098437725E-5	1.4505466929167266E-4	0.0	3.1400256239073303	0.0
78-79	5.439550098437725E-5	1.541205861224022E-4	0.0	3.4074520385801903	1.8131833661459082E-5
80-81	5.439550098437725E-5	1.6318650295313176E-4	0.0	3.692547925156142	1.8131833661459082E-5
82-83	5.439550098437725E-5	1.6318650295313176E-4	0.0	3.986102312135165	1.8131833661459082E-5
84-85	5.439550098437725E-5	1.6318650295313176E-4	0.0	4.282086364824823	1.8131833661459082E-5
86-87	5.439550098437725E-5	1.8131833661459083E-4	0.0	4.592992916617861	1.8131833661459082E-5
88	5.439550098437725E-5	1.8131833661459083E-4	0.0	4.841942992789695	1.8131833661459082E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5640	0.0	45.760406	1
GTATCAA	9175	0.0	33.919434	1
TCAACGC	9480	0.0	31.637136	4
ATCAACG	9680	0.0	31.177729	3
CAACGCA	10130	0.0	29.839142	5
AACGCAG	10600	0.0	28.512722	6
TATCAAC	11080	0.0	27.535294	2
ACGCAGA	12490	0.0	23.708271	7
CGCAGAG	12630	0.0	22.961678	8
GTACATG	14785	0.0	22.893269	1
TACATGG	14730	0.0	21.605818	2
ACATGGG	15275	0.0	19.91168	3
GCAGAGT	14705	0.0	18.634825	9
GTGGTAT	3070	0.0	18.222383	1
CAGAGTA	14010	0.0	17.596617	10-11
GAGTACA	11605	0.0	16.730215	1
CATGGGG	11415	0.0	16.678766	4
AGAGTAC	12885	0.0	16.123512	10-11
GTATTAG	2650	0.0	15.965895	1
TATACTG	3365	0.0	15.646521	5
>>END_MODULE