FastQCFastQC Report
Wed 25 May 2016
SRR2049363_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049363_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5515162
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108560.19683918622879984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107230.19442765235182574No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61430.11138385418234314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85500.057.1313821
GTATCAA153800.039.7691461
ATCAACG191200.031.5108453
TCAACGC191050.031.4863874
CAACGCA197600.030.3951175
TATCAAC200100.030.3206882
AACGCAG203700.029.5382376
GTGGTAT37400.026.0210041
ACGCAGA235650.025.3736637
TGGTATC36850.024.8689252
CGCAGAG239350.024.8438388
GTACATG142350.022.5903991
GCAGAGT264900.021.9856769
TACATGG143700.021.257712
GAGTACT186200.020.63102712-13
ACATGGG147850.020.470313
AGAGTAC242150.020.40857510-11
CAGAGTA256150.020.23896810-11
AGTACTT193850.018.90726912-13
GTACTTT202250.018.83304214-15
Produced by FastQC (version 0.11.5)