##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049363_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5515162 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.824197367185224 31.0 31.0 34.0 30.0 34.0 2 31.893569037500622 33.0 31.0 34.0 30.0 34.0 3 31.9010941111068 33.0 31.0 34.0 30.0 34.0 4 35.661650917960344 37.0 35.0 37.0 33.0 37.0 5 35.77350148554113 37.0 35.0 37.0 35.0 37.0 6 35.845871435870784 37.0 35.0 37.0 35.0 37.0 7 35.78310283541988 37.0 35.0 37.0 33.0 37.0 8 35.78052738976661 37.0 35.0 37.0 33.0 37.0 9 37.50995909095689 39.0 37.0 39.0 35.0 39.0 10-11 37.47891730106931 39.0 37.0 39.0 34.5 39.0 12-13 37.436685178060046 39.0 37.0 39.0 34.0 39.0 14-15 38.73416392845758 40.0 38.0 41.0 34.5 41.0 16-17 38.76243082252162 40.0 38.0 41.0 35.0 41.0 18-19 38.82361053038878 40.0 38.0 41.0 35.0 41.0 20-21 38.85221331667138 40.0 38.0 41.0 35.0 41.0 22-23 38.825649545743175 40.0 38.0 41.0 35.0 41.0 24-25 38.81126583407704 40.0 38.0 41.0 35.0 41.0 26-27 38.70533494755004 40.0 38.0 41.0 34.5 41.0 28-29 38.62034787010789 40.0 38.0 41.0 34.0 41.0 30-31 38.517847435850484 40.0 38.0 41.0 34.0 41.0 32-33 38.38918068045871 40.0 38.0 41.0 34.0 41.0 34-35 38.29443795123335 40.0 38.0 41.0 34.0 41.0 36-37 38.18637285722523 40.0 38.0 41.0 34.0 41.0 38-39 38.09494662169489 40.0 38.0 41.0 33.5 41.0 40-41 37.979887444829366 40.0 38.0 41.0 33.0 41.0 42-43 37.8426709859112 40.0 37.0 41.0 33.0 41.0 44-45 37.67351729650008 40.0 37.0 41.0 33.0 41.0 46-47 37.453328025541225 40.0 37.0 41.0 32.5 41.0 48-49 37.253744586287766 40.0 37.0 41.0 32.0 41.0 50-51 37.10972279690061 40.0 37.0 41.0 31.5 41.0 52-53 36.97104001659425 40.0 36.0 41.0 31.0 41.0 54-55 36.83010616913882 40.0 36.0 41.0 31.0 41.0 56-57 36.68863398754198 39.0 36.0 41.0 31.0 41.0 58-59 36.50043199093699 39.0 35.0 41.0 31.0 41.0 60-61 36.31846408138148 39.0 35.0 41.0 31.0 41.0 62-63 36.116911434333204 39.0 35.0 41.0 30.0 41.0 64-65 35.885625118536865 38.0 35.0 40.0 30.0 41.0 66-67 35.61641616329675 37.5 35.0 40.0 30.0 41.0 68-69 35.32315170433797 37.0 35.0 40.0 29.0 41.0 70-71 34.98827958272123 37.0 34.0 39.5 29.0 41.0 72-73 34.69060410192847 36.0 34.0 39.0 29.0 41.0 74-75 34.384964394518235 36.0 34.0 39.0 29.0 40.5 76-77 34.05763411845382 35.0 34.0 38.5 29.0 40.0 78-79 33.722634711364776 35.0 34.0 37.0 29.0 39.0 80-81 33.408426896617 35.0 34.0 37.0 28.0 39.0 82-83 33.10909407556841 35.0 34.0 36.0 28.0 39.0 84-85 32.844013013579655 35.0 33.0 36.0 28.0 37.0 86-87 32.61631761678079 35.0 33.0 36.0 27.5 37.0 88-89 32.391913963724 35.0 33.0 35.0 27.0 37.0 90-91 32.19976140320085 35.0 33.0 35.0 27.0 36.0 92-93 32.024585497216584 35.0 33.0 35.0 26.0 36.0 94-95 31.86977454152752 35.0 33.0 35.0 26.0 36.0 96-97 31.676478950210345 35.0 33.0 35.0 26.0 36.0 98-99 31.534885829282985 35.0 33.0 35.0 25.5 35.0 100 31.4230040749483 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1792915499997072 1101 2 0.18463509342045725 1101 3 0.37370288510519245 1101 4 0.2236075201729193 1101 5 0.19507081853354435 1101 6 0.15138632529012597 1101 7 0.07064824706665718 1101 8 0.10394766166005098 1101 9 0.19316429464664253 1101 10-11 0.23386326307127092 1101 12-13 0.16064812013909346 1101 14-15 0.29764211496515003 1101 16-17 0.20150064635814147 1101 18-19 0.26648841033179593 1101 20-21 0.2534354582581244 1101 22-23 0.2416128353385858 1101 24-25 0.2655716096242955 1101 26-27 0.3396165665081341 1101 28-29 0.34773331544918307 1101 30-31 0.31277023340307863 1101 32-33 0.38020274534595444 1101 34-35 0.4046005099364862 1101 36-37 0.4589488476986858 1101 38-39 0.3862725713066979 1101 40-41 0.40042255658877224 1101 42-43 0.4583497653886113 1101 44-45 0.45113252632961576 1101 46-47 0.48878780695288526 1101 48-49 0.5626954895385694 1101 50-51 0.4594388117567618 1101 52-53 0.5484270468791266 1101 54-55 0.5124232132717523 1101 56-57 0.494306480503127 1101 58-59 0.5037398144197667 1101 60-61 0.5179646777028566 1101 62-63 0.5303302059055852 1101 64-65 0.4795939065581649 1101 66-67 0.4137240605974952 1101 68-69 0.44043496734094134 1101 70-71 0.4522944913581881 1101 72-73 0.44963858817352076 1101 74-75 0.46903275112616427 1101 76-77 0.40443140221812257 1101 78-79 0.4599606242082288 1101 80-81 0.47873527137318206 1101 82-83 0.4455886668624771 1101 84-85 0.4441685551493464 1101 86-87 0.4009239875860544 1101 88-89 0.5080077826912586 1101 90-91 0.5159899740241229 1101 92-93 0.5382139733128994 1101 94-95 0.5496588023349531 1101 96-97 0.5723607721551751 1101 98-99 0.5538960558877548 1101 100 0.5062190990751176 1102 1 0.2568731149762655 1102 2 0.2183411684637484 1102 3 0.40165892816778737 1102 4 0.239570283377887 1102 5 0.18501970589726113 1102 6 0.22715115693860355 1102 7 0.2260511085995418 1102 8 0.23992310737715172 1102 9 0.20199766786078754 1102 10-11 0.2814568500457 1102 12-13 0.2524630017936005 1102 14-15 0.32535861790139364 1102 16-17 0.24755817868258134 1102 18-19 0.3076467296350529 1102 20-21 0.26742850090497683 1102 22-23 0.28811108543845165 1102 24-25 0.32140152234993735 1102 26-27 0.37465495786296543 1102 28-29 0.3614011345909347 1102 30-31 0.33570648768154143 1102 32-33 0.3575556415590313 1102 34-35 0.3230651209061861 1102 36-37 0.33483377328568764 1102 38-39 0.3656741785644684 1102 40-41 0.3461503726347175 1102 42-43 0.3588635609311126 1102 44-45 0.37875987419858603 1102 46-47 0.42960463559285955 1102 48-49 0.3105325286707199 1102 50-51 0.38076873043342374 1102 52-53 0.43110383009295106 1102 54-55 0.3772819779307426 1102 56-57 0.3867341853816555 1102 58-59 0.327895599805224 1102 60-61 0.38297526491695777 1102 62-63 0.38524242339972403 1102 64-65 0.39923878792198764 1102 66-67 0.2853822054069184 1102 68-69 0.3377298885893012 1102 70-71 0.42077904220066387 1102 72-73 0.44218611257402074 1102 74-75 0.38264049218747687 1102 76-77 0.4004539256908686 1102 78-79 0.4439505560562438 1102 80-81 0.4216630254392726 1102 82-83 0.4380515927868913 1102 84-85 0.42401778845979976 1102 86-87 0.4207096765520504 1102 88-89 0.4200241310894981 1102 90-91 0.40507189492852547 1102 92-93 0.4358621420594275 1102 94-95 0.43541274443249023 1102 96-97 0.4560648066241946 1102 98-99 0.43277562393822677 1102 100 0.4709087401724048 1103 1 0.17157878891366352 1103 2 0.12308564199878802 1103 3 0.2656636111003614 1103 4 0.19925228179275933 1103 5 0.21681966655828688 1103 6 0.16134721526133688 1103 7 0.1969427201148406 1103 8 0.19311137515902743 1103 9 0.21682939452811212 1103 10-11 0.2025217079679038 1103 12-13 0.21702709317812463 1103 14-15 0.23158910098968732 1103 16-17 0.24638123604387374 1103 18-19 0.24451943756316297 1103 20-21 0.28190089115049943 1103 22-23 0.2518616646107503 1103 24-25 0.26995644218992965 1103 26-27 0.24932660803419537 1103 28-29 0.2879886906795548 1103 30-31 0.2485907328496566 1103 32-33 0.2311371906926567 1103 34-35 0.22294155653969483 1103 36-37 0.21537465857326055 1103 38-39 0.2366973172092557 1103 40-41 0.26983570554791214 1103 42-43 0.32547193800130003 1103 44-45 0.33427288981564374 1103 46-47 0.30390548156337616 1103 48-49 0.22201358036907948 1103 50-51 0.20688377293893012 1103 52-53 0.24332128669757225 1103 54-55 0.19668136126710323 1103 56-57 0.15595621460900588 1103 58-59 0.2519526500800211 1103 60-61 0.26359674426317525 1103 62-63 0.2585375291799821 1103 64-65 0.2845639285989847 1103 66-67 0.25841771324217433 1103 68-69 0.20557614809050762 1103 70-71 0.27876696126465816 1103 72-73 0.2875764063677124 1103 74-75 0.24565138211816162 1103 76-77 0.21837900315279057 1103 78-79 0.2453425967876015 1103 80-81 0.30297825594635697 1103 82-83 0.32766155072121705 1103 84-85 0.2832947634913623 1103 86-87 0.26972331667985117 1103 88-89 0.303085165424406 1103 90-91 0.2921643215434244 1103 92-93 0.3356044647961838 1103 94-95 0.2737596380021827 1103 96-97 0.32708914759749774 1103 98-99 0.2950554144274591 1103 100 0.34524827372616684 1104 1 0.159396157425995 1104 2 0.17553468469853684 1104 3 0.27229444104417766 1104 4 0.13907579234089695 1104 5 0.16587627341890965 1104 6 0.1750076820822386 1104 7 0.18594260231341053 1104 8 0.16139620266173438 1104 9 0.1660545228825896 1104 10-11 0.1941866430625936 1104 12-13 0.21673954315341604 1104 14-15 0.2516065503268905 1104 16-17 0.24098527597855934 1104 18-19 0.29165780352639814 1104 20-21 0.31792236046157996 1104 22-23 0.285487838125718 1104 24-25 0.2412450017074761 1104 26-27 0.2835424022612685 1104 28-29 0.35903502100131846 1104 30-31 0.3284965080648874 1104 32-33 0.30368712541103093 1104 34-35 0.23176746074749843 1104 36-37 0.2846077467050989 1104 38-39 0.2906626443209248 1104 40-41 0.29245685286841905 1104 42-43 0.3558618443818844 1104 44-45 0.3401367833493225 1104 46-47 0.37584943425955686 1104 48-49 0.356813187043187 1104 50-51 0.2858317979651872 1104 52-53 0.26407438143937156 1104 54-55 0.2828801053729393 1104 56-57 0.3064622699298667 1104 58-59 0.3641740578725887 1104 60-61 0.36074202287992563 1104 62-63 0.3280951848005813 1104 64-65 0.2255991986751269 1104 66-67 0.3011573409896471 1104 68-69 0.24220237232552932 1104 70-71 0.24615641607308447 1104 72-73 0.28674197955349 1104 74-75 0.3108753357305716 1104 76-77 0.23227916316639607 1104 78-79 0.25564715439413277 1104 80-81 0.2764267129112028 1104 82-83 0.2743676508720654 1104 84-85 0.32246026305124786 1104 86-87 0.3021894733099444 1104 88-89 0.3008025581514815 1104 90-91 0.3063346476897806 1104 92-93 0.38944790014773645 1104 94-95 0.42979827032755225 1104 96-97 0.5292394736784125 1104 98-99 0.5086803363022057 1104 100 0.4904021354861854 1105 1 0.16332798858048037 1105 2 0.15650569279041449 1105 3 0.29142806787032427 1105 4 0.1563527485345375 1105 5 0.12637040286711 1105 6 0.11840683155909915 1105 7 0.10682883441086233 1105 8 0.14244294489261478 1105 9 0.19735465868523505 1105 10-11 0.19251390121721101 1105 12-13 0.1589283843962761 1105 14-15 0.21337494331838514 1105 16-17 0.18142745282246153 1105 18-19 0.20382909029509477 1105 20-21 0.22315129241398068 1105 22-23 0.2209180841641114 1105 24-25 0.17733685706756575 1105 26-27 0.2051956539851929 1105 28-29 0.20455585279562882 1105 30-31 0.18071673076048 1105 32-33 0.26116342846397345 1105 34-35 0.2585987677743731 1105 36-37 0.1907403496259903 1105 38-39 0.21129832781110025 1105 40-41 0.22217576924879978 1105 42-43 0.2771085467325065 1105 44-45 0.22508423412016043 1105 46-47 0.2838214589821604 1105 48-49 0.23465122944595862 1105 50-51 0.14890359353422156 1105 52-53 0.1619877085387884 1105 54-55 0.1542119621067286 1105 56-57 0.17462863138551654 1105 58-59 0.2249024725433344 1105 60-61 0.19086565449148196 1105 62-63 0.2129416802159838 1105 64-65 0.15253029656750527 1105 66-67 0.20840929222927684 1105 68-69 0.19348886160326373 1105 70-71 0.2727358140413827 1105 72-73 0.24792741563607734 1105 74-75 0.2197341914311579 1105 76-77 0.18227815093423771 1105 78-79 0.20503971218131056 1105 80-81 0.2570658554721774 1105 82-83 0.24321036245650163 1105 84-85 0.19457846768860776 1105 86-87 0.14645596195157395 1105 88-89 0.20839068889194579 1105 90-91 0.22995100950068093 1105 92-93 0.2085535115455528 1105 94-95 0.2206015602907314 1105 96-97 0.2631642894154993 1105 98-99 0.25400935715057926 1105 100 0.2647255245454829 1106 1 0.16158908043261277 1106 2 0.10375553604228571 1106 3 0.26712189514009665 1106 4 0.18814773847258692 1106 5 0.13261305460903827 1106 6 0.180382853795507 1106 7 0.1796177863640338 1106 8 0.13972247475071242 1106 9 0.19657813010331893 1106 10-11 0.17034255760622585 1106 12-13 0.12072416240223305 1106 14-15 0.19252950189751772 1106 16-17 0.24997367388752423 1106 18-19 0.24139495013453427 1106 20-21 0.274483619292063 1106 22-23 0.20914086749912997 1106 24-25 0.21171601058760103 1106 26-27 0.2807794368373919 1106 28-29 0.2732546848056785 1106 30-31 0.2949189633751814 1106 32-33 0.23204224228669545 1106 34-35 0.23402438723070418 1106 36-37 0.28729567833956793 1106 38-39 0.2807387514047335 1106 40-41 0.2579167733450518 1106 42-43 0.30354532654721567 1106 44-45 0.2670042879232781 1106 46-47 0.3507544283426043 1106 48-49 0.3818244040760348 1106 50-51 0.3328809783610822 1106 52-53 0.29801959861274696 1106 54-55 0.2746363607705291 1106 56-57 0.2746188840512147 1106 58-59 0.35500934093185066 1106 60-61 0.318732326163051 1106 62-63 0.30548037411255535 1106 64-65 0.21554671098343903 1106 66-67 0.22425941488209844 1106 68-69 0.2697640535582124 1106 70-71 0.3022153980256945 1106 72-73 0.33131584018787663 1106 74-75 0.2854308037505504 1106 76-77 0.23790924229696486 1106 78-79 0.2629378868843659 1106 80-81 0.23580181491566066 1106 82-83 0.34049894050166074 1106 84-85 0.2951139737452948 1106 86-87 0.29263799694925297 1106 88-89 0.2950815048734725 1106 90-91 0.312311341434075 1106 92-93 0.356677580720941 1106 94-95 0.3542263814566695 1106 96-97 0.44901001965883935 1106 98-99 0.4399974690681674 1106 100 0.4273598244510026 1107 1 0.12062656190818544 1107 2 0.08218129509677397 1107 3 0.2006415102844592 1107 4 0.1444013540562068 1107 5 0.12058079031988456 1107 6 0.10731108048478433 1107 7 0.07772247595467974 1107 8 0.07061983864241483 1107 9 0.1574059494194202 1107 10-11 0.10483301478713258 1107 12-13 0.1538977826530683 1107 14-15 0.21684821997582304 1107 16-17 0.150609286937609 1107 18-19 0.15374106416280853 1107 20-21 0.1460940285112855 1107 22-23 0.11258293958931631 1107 24-25 0.14268911392895944 1107 26-27 0.2349270141401547 1107 28-29 0.16384366350185076 1107 30-31 0.17450235262202796 1107 32-33 0.23302496463104205 1107 34-35 0.14789882226104822 1107 36-37 0.1775127854919134 1107 38-39 0.16296914752045666 1107 40-41 0.16596224912020574 1107 42-43 0.1595261991853434 1107 44-45 0.08573613040511674 1107 46-47 0.1574261398836896 1107 48-49 0.15764462125548562 1107 50-51 0.16797326500326193 1107 52-53 0.10858994824337032 1107 54-55 0.0718332250326057 1107 56-57 0.10605281862806493 1107 58-59 0.11798063045336704 1107 60-61 0.07442040327585886 1107 62-63 0.07293950794326776 1107 64-65 0.1100768408303594 1107 66-67 0.1443583219269442 1107 68-69 0.12335328292821401 1107 70-71 0.09507328334284182 1107 72-73 0.14793275571636144 1107 74-75 0.11266252553881628 1107 76-77 0.07968617548870327 1107 78-79 0.1546205172309456 1107 80-81 0.17848093509893204 1107 82-83 0.17559051737670472 1107 84-85 0.17177289687466413 1107 86-87 0.12208263335512015 1107 88-89 0.15368145139550649 1107 90-91 0.17179122009971337 1107 92-93 0.2552963539819828 1107 94-95 0.2668629030086862 1107 96-97 0.2526121541873039 1107 98-99 0.27765254572400977 1107 100 0.2690088801588999 1108 1 0.08904758259824419 1108 2 0.053031650148355425 1108 3 0.14749778072387798 1108 4 0.06695957225973359 1108 5 0.04570323178784008 1108 6 2.2862308023974265E-4 1108 7 -0.027814049724945278 1108 8 -0.04477069219845475 1108 9 0.06873085051125116 1108 10-11 0.0749674175249595 1108 12-13 0.03794886171785805 1108 14-15 0.11570558945962972 1108 16-17 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2215 10-11 -0.31536976348048995 2215 12-13 -0.282574737708444 2215 14-15 -0.3689121177151904 2215 16-17 -0.4046931770675002 2215 18-19 -0.38836130081585907 2215 20-21 -0.4095391589506363 2215 22-23 -0.351522756899044 2215 24-25 -0.3508253158253538 2215 26-27 -0.4362453270368505 2215 28-29 -0.40418486382881724 2215 30-31 -0.37157305734164936 2215 32-33 -0.36284824334837396 2215 34-35 -0.42336533053620684 2215 36-37 -0.4672556273384316 2215 38-39 -0.4884691246064605 2215 40-41 -0.4476413711430638 2215 42-43 -0.4182416486967142 2215 44-45 -0.48181964645194597 2215 46-47 -0.5570584854879783 2215 48-49 -0.45981430463657347 2215 50-51 -0.45691920849522916 2215 52-53 -0.4645025688600555 2215 54-55 -0.350637264644746 2215 56-57 -0.4307779892399566 2215 58-59 -0.4111597975150332 2215 60-61 -0.4546707409289965 2215 62-63 -0.46880363288033067 2215 64-65 -0.43241566272112664 2215 66-67 -0.3836718891141402 2215 68-69 -0.4291087159735909 2215 70-71 -0.43419325990187474 2215 72-73 -0.3878800016927144 2215 74-75 -0.425624143888399 2215 76-77 -0.3080221830476262 2215 78-79 -0.4012574527349173 2215 80-81 -0.4369684658672952 2215 82-83 -0.3991799987123841 2215 84-85 -0.42768734905238404 2215 86-87 -0.3245087194954124 2215 88-89 -0.35492282539946274 2215 90-91 -0.36964643160725785 2215 92-93 -0.4128545244764936 2215 94-95 -0.45037489859214475 2215 96-97 -0.4328790669456133 2215 98-99 -0.3748844076318427 2215 100 -0.3270638640553436 2216 1 -0.5541422024813691 2216 2 -0.5623965809757685 2216 3 -0.6515543461810935 2216 4 -0.39839249490022866 2216 5 -0.5639935682507726 2216 6 -0.510164948425242 2216 7 -0.7054152264218416 2216 8 -0.46469237714646283 2216 9 -0.7654553428806565 2216 10-11 -0.6123574416349982 2216 12-13 -0.5842357446711972 2216 14-15 -0.8804136862903462 2216 16-17 -0.7348886081307455 2216 18-19 -0.6617750463935366 2216 20-21 -0.6969855279506305 2216 22-23 -0.7052050401375567 2216 24-25 -0.6682306014240069 2216 26-27 -0.8125961489722471 2216 28-29 -0.9055224848014376 2216 30-31 -1.0043225237888151 2216 32-33 -0.9763465494476193 2216 34-35 -0.9195842781955577 2216 36-37 -1.0141771544659122 2216 38-39 -0.8975021883393808 2216 40-41 -0.9399485100900407 2216 42-43 -1.0170206515461118 2216 44-45 -1.2105356213881606 2216 46-47 -1.1545262692903009 2216 48-49 -1.2009610121159398 2216 50-51 -1.0388990880556648 2216 52-53 -1.0006189826363823 2216 54-55 -0.9202875076427262 2216 56-57 -1.0792033274352661 2216 58-59 -1.1713519433766422 2216 60-61 -1.0144464757881622 2216 62-63 -0.9521200806783767 2216 64-65 -0.9955197529809539 2216 66-67 -0.8857405342876348 2216 68-69 -0.922879031252684 2216 70-71 -0.9480112423536156 2216 72-73 -0.9152257751789037 2216 74-75 -0.9209841308010596 2216 76-77 -0.8890453270089722 2216 78-79 -1.0982326944127934 2216 80-81 -1.2490519880245259 2216 82-83 -1.0977452671130195 2216 84-85 -1.2582521355343275 2216 86-87 -1.0313718791508606 2216 88-89 -1.454182535459534 2216 90-91 -1.2639161819185674 2216 92-93 -1.3914336438619905 2216 94-95 -1.3391969332381564 2216 96-97 -1.3424457879534621 2216 98-99 -1.466981968762326 2216 100 -1.4533645348647752 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 269.0 3 26.0 4 49.0 5 90.0 6 219.0 7 631.0 8 1449.0 9 2439.0 10 3985.0 11 5788.0 12 8110.0 13 11458.0 14 14097.0 15 18197.0 16 22542.0 17 30485.0 18 29631.0 19 16525.0 20 13043.0 21 12953.0 22 13842.0 23 15399.0 24 18717.0 25 23099.0 26 29883.0 27 38239.0 28 50807.0 29 66683.0 30 86720.0 31 112724.0 32 145653.0 33 190462.0 34 260972.0 35 376343.0 36 615355.0 37 1107719.0 38 1546276.0 39 622324.0 40 1959.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.03010211309183 20.742686508255368 14.939197754625672 23.288013624027133 2 15.52826553417651 20.452363865286276 40.03604971168571 23.983320888851498 3 17.831751814361933 25.393542383705135 30.759894269651554 26.014811532281374 4 12.74659996079894 17.400048629621967 38.07425609098708 31.77909531859202 5 12.809034439967492 37.75588096958893 35.67998546552214 13.755099124921443 6 30.800914062164274 38.16657392232067 17.193550650651904 13.838961364863147 7 26.583280781235434 32.9016264617431 22.41595079165399 18.099141965367473 8 24.820199389425866 36.59361499088781 20.1044688463768 18.481716773309532 9 24.66991903410997 16.721866012276703 21.6075611196915 37.00065383392183 10-11 23.810170943301394 27.07416391395212 28.693200671167958 20.422464471578532 12-13 24.493004806403775 24.530735524986216 29.03367004069385 21.94258962791616 14-15 21.73637321080553 26.50411642897169 26.650730582169334 25.108779778053442 16-17 20.844274383961885 29.8557231863724 27.4182426554288 21.881759774236915 18-19 20.692318989838917 28.268091950188207 30.22867876906563 20.810910290907245 20-21 21.731515059811972 27.416764178218994 30.080667672560995 20.77105308940804 22-23 21.388165932387842 27.300131890958053 30.12832623955561 21.183375937098493 24-25 21.33321015757582 27.423773894276216 30.03778951251502 21.205226435632945 26-27 21.076471621750752 27.402982362968086 30.106776652051792 21.41376936322937 28-29 21.113713642601834 27.38164722111466 30.254854679198708 21.249784457084793 30-31 21.41995103679638 27.651835068489376 29.86712810974546 21.061085784968782 32-33 21.13881047104888 27.433562771054532 30.186842623214478 21.240784134682116 34-35 21.291970004853894 27.275659686329984 30.10402782585235 21.328342482963773 36-37 21.52464562658869 27.544673705571753 29.61640076610693 21.314279901732625 38-39 21.426280983418845 27.656540464239626 29.719087295489377 21.198091256852152 40-41 21.368726593475014 27.458672742772166 29.762116408141164 21.41048425561165 42-43 21.34820041641758 27.476316544811123 29.611268042842813 21.56421499592848 44-45 21.49536345129482 27.55683324879344 29.449964846815067 21.497838453096676 46-47 21.591313245836858 27.709653880112718 29.08794684030215 21.61108603374828 48-49 21.61272863262392 27.735182792774417 28.855873620271527 21.796214954330136 50-51 21.671329288828183 27.746373299520936 28.6785713573389 21.903726054311978 52-53 21.603874867160908 27.809303169770583 28.71471230692435 21.872109656144158 54-55 21.686161195995677 28.0788491745536 28.51470416782031 21.720285461630418 56-57 21.571011977408343 27.846608594933247 28.53699992366458 22.04537950399383 58-59 21.472242688572145 27.55518639065244 28.855984981733503 22.116585939041915 60-61 21.64401889740251 27.849199736108694 28.540051362161623 21.96673000432717 62-63 21.444626640523992 27.910577837782512 28.59749845900919 22.047297062684308 64-65 21.47841544027621 27.679587868618615 28.682963216944213 22.159033474160967 66-67 21.451440516844116 27.71112474845136 28.55765725523979 22.279777479464737 68-69 21.748921367485725 27.795383026734854 28.440132835667992 22.01556277011143 70-71 21.761077528305943 27.605479860395217 28.460834316007 22.172608295291845 72-73 21.56431719335706 27.763906304410124 28.468506235272862 22.203270266959958 74-75 21.641993405938067 27.792945693683368 28.326796900916 22.238263999462568 76-77 21.680313530489922 27.73764622400705 28.203129738389453 22.378910507113574 78-79 21.691419024472637 27.80562961372686 28.261604517790715 22.241346844009783 80-81 21.612601293811938 27.671671603789 28.315640139901554 22.400086962497515 82-83 21.51182482969453 27.69245832796926 28.397551082575262 22.398165759760946 84-85 21.55406117220587 27.594826928129557 28.279148891726795 22.571963007937775 86-87 21.60227419844405 27.68923276989697 28.203222941582418 22.505270090076564 88-89 21.470984556906917 27.554632506351478 28.300055783673578 22.674327153068024 90-91 21.39790444432961 27.42705582315882 28.35893167821244 22.816108054299132 92-93 21.427286628125934 27.62502150939859 28.200258529825128 22.747433332650353 94-95 21.297423783549043 27.432301278294023 28.31718409945299 22.953090838703947 96-97 21.328496820258593 27.395204979879846 28.29572297695781 22.98057522290375 98-99 21.29091837467389 27.465437921713203 28.2397256647502 23.003918038862707 100 21.338732102720467 27.28370048398175 28.25340466255818 23.124162750739607 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 459.0 1 338.0 2 358.5 3 533.5 4 382.0 5 254.0 6 616.5 7 1764.0 8 3065.5 9 3521.5 10 2875.0 11 2076.5 12 2078.5 13 2418.0 14 2826.5 15 3339.5 16 3828.0 17 4426.5 18 5217.5 19 6084.0 20 7275.0 21 9028.0 22 11517.5 23 15478.0 24 21060.0 25 28244.0 26 37938.0 27 50068.0 28 64352.5 29 80742.5 30 97466.5 31 112115.0 32 124430.5 33 136385.5 34 148586.0 35 159324.5 36 168646.5 37 177940.5 38 187076.5 39 193009.0 40 196911.0 41 199529.5 42 199598.0 43 199594.0 44 197876.0 45 195041.5 46 191464.5 47 185030.5 48 178219.0 49 172636.0 50 168041.0 51 161513.5 52 150976.5 53 141727.0 54 134602.0 55 127508.0 56 117794.0 57 106258.5 58 97122.5 59 89038.0 60 78794.5 61 67200.5 62 57729.5 63 48802.0 64 39813.0 65 32070.5 66 25405.0 67 19723.0 68 15250.5 69 11853.0 70 8918.0 71 6484.5 72 4772.5 73 3509.0 74 2596.5 75 1798.0 76 1135.0 77 739.0 78 485.5 79 311.0 80 175.5 81 81.5 82 44.5 83 20.0 84 15.0 85 12.0 86 8.0 87 4.0 88 1.0 89 2.0 90 2.5 91 1.0 92 0.5 93 0.0 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03555652581012126 2 0.0 3 0.0 4 9.065916830729542E-5 5 0.0 6 1.8131833661459082E-5 7 0.0 8 4.5329584153647705E-4 9 0.0 10-11 0.0 12-13 0.04374304870827004 14-15 9.972508513802495E-5 16-17 0.0 18-19 3.6263667322918165E-5 20-21 6.34614178151068E-5 22-23 0.0 24-25 1.3598875246094314E-4 26-27 9.065916830729542E-5 28-29 3.4450483956772256E-4 30-31 0.0 32-33 2.2664792076823852E-4 34-35 5.4395500984377244E-5 36-37 9.065916830729542E-5 38-39 2.629115880911567E-4 40-41 1.1785691879948404E-4 42-43 4.5329584153647705E-4 44-45 2.629115880911567E-4 46-47 1.1785691879948404E-4 48-49 6.70877845473986E-4 50-51 4.5329584153647705E-4 52-53 5.620868435052316E-4 54-55 4.5329584153647705E-4 56-57 5.258231761823134E-4 58-59 2.0851608710677945E-4 60-61 2.2664792076823852E-4 62-63 7.52471096950552E-4 64-65 4.986254256901248E-4 66-67 0.0013689534414401607 68-69 0.0013961511919323493 70-71 3.898344237213703E-4 72-73 8.431302652578475E-4 74-75 8.793939325807657E-4 76-77 6.618119286432566E-4 78-79 0.003000818470971478 80-81 0.002556588546265731 82-83 0.0013054920236250538 84-85 0.002556588546265731 86-87 0.0013780193582708902 88-89 0.0019491721186068512 90-91 0.002846697884849076 92-93 0.00237527020965114 94-95 0.003526641647153792 96-97 0.003073345805617314 98-99 0.002864829718510535 100 0.0014142830255938084 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5515162.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.64895953504463 #Duplication Level Percentage of deduplicated Percentage of total 1 75.54827812648706 45.81924463035611 2 14.600182587806657 17.70971765944298 3 4.572059788760353 8.318720073609942 4 1.8938771974331587 4.594467260458695 5 0.9589470078398554 2.907956913736576 6 0.5887489405705073 2.142420640377666 7 0.36107201096194513 1.5329049247447741 8 0.25527924892442894 1.238593667052342 9 0.2046098803776543 1.1168438719945208 >10 0.9524385786020075 10.063879747195696 >50 0.04586644189295887 1.8891885551461276 >100 0.017922259505880854 1.7739838387034916 >500 4.4870748496335345E-4 0.20353969864497085 >1k 1.7948223509577476E-4 0.18559625435686314 >5k 2.9913705842697035E-5 0.1114484643551493 >10k+ 5.982741168539407E-5 0.3914937998241521 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10856 0.19683918622879984 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10723 0.19442765235182574 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6143 0.11138385418234314 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.6263667322918165E-5 0.0 5 0.0 0.0 0.0 3.6263667322918165E-5 0.0 6 0.0 0.0 0.0 3.6263667322918165E-5 0.0 7 0.0 0.0 0.0 3.6263667322918165E-5 0.0 8 1.8131833661459082E-5 0.0 0.0 3.6263667322918165E-5 0.0 9 1.8131833661459082E-5 0.0 0.0 9.065916830729542E-5 0.0 10-11 1.8131833661459082E-5 0.0 0.0 9.972508513802496E-5 0.0 12-13 1.8131833661459082E-5 0.0 0.0 1.1785691879948404E-4 0.0 14-15 2.7197750492188622E-5 0.0 0.0 1.269228356302136E-4 0.0 16-17 3.6263667322918165E-5 0.0 0.0 1.541205861224022E-4 0.0 18-19 3.6263667322918165E-5 1.8131833661459082E-5 0.0 2.0851608710677945E-4 0.0 20-21 3.6263667322918165E-5 1.8131833661459082E-5 0.0 3.173070890755339E-4 0.0 22-23 3.6263667322918165E-5 1.8131833661459082E-5 0.0 4.804935920286657E-4 0.0 24-25 3.6263667322918165E-5 1.8131833661459082E-5 0.0 8.612620989193063E-4 0.0 26-27 3.6263667322918165E-5 1.8131833661459082E-5 0.0 0.0017587878651615311 0.0 28-29 3.6263667322918165E-5 1.8131833661459082E-5 0.0 0.00513130892619292 0.0 30-31 4.532958415364771E-5 1.8131833661459082E-5 0.0 0.015983211372576182 0.0 32-33 6.34614178151068E-5 1.8131833661459082E-5 0.0 0.039853770387887066 0.0 34-35 7.252733464583633E-5 1.8131833661459082E-5 0.0 0.07745012748492247 0.0 36-37 7.252733464583633E-5 1.8131833661459082E-5 0.0 0.13714012389844576 0.0 38-39 7.252733464583633E-5 1.8131833661459082E-5 0.0 0.2161042594941001 0.0 40-41 7.252733464583633E-5 1.8131833661459082E-5 0.0 0.2973530061310983 0.0 42-43 7.252733464583633E-5 1.8131833661459082E-5 0.0 0.3768520308197656 0.0 44-45 7.252733464583633E-5 1.8131833661459082E-5 0.0 0.47566145835788687 0.0 46-47 7.252733464583633E-5 1.8131833661459082E-5 0.0 0.5909799204447665 0.0 48-49 7.252733464583633E-5 1.8131833661459082E-5 0.0 0.7090453553313574 0.0 50-51 7.252733464583633E-5 1.8131833661459082E-5 0.0 0.8258596936953075 0.0 52-53 7.252733464583633E-5 1.8131833661459082E-5 0.0 0.9378418983884789 0.0 54-55 7.252733464583633E-5 2.7197750492188622E-5 0.0 1.0614919380427992 0.0 56-57 7.252733464583633E-5 3.6263667322918165E-5 0.0 1.2102998969023937 0.0 58-59 8.159325147656587E-5 3.6263667322918165E-5 0.0 1.3678292677531503 0.0 60-61 9.065916830729542E-5 3.6263667322918165E-5 0.0 1.529139125922321 0.0 62-63 9.972508513802496E-5 5.439550098437725E-5 0.0 1.6903673908400152 0.0 64-65 1.087910019687545E-4 5.439550098437725E-5 0.0 1.8595917944024127 0.0 66-67 1.087910019687545E-4 5.439550098437725E-5 0.0 2.0503749481882854 0.0 68-69 1.087910019687545E-4 5.439550098437725E-5 0.0 2.2592990015524475 0.0 70-71 1.1785691879948404E-4 5.439550098437725E-5 0.0 2.4781864975135814 0.0 72-73 1.269228356302136E-4 5.439550098437725E-5 0.0 2.7019423908128175 0.0 74-75 1.269228356302136E-4 5.439550098437725E-5 0.0 2.924429055755751 0.0 76-77 1.3598875246094314E-4 7.252733464583633E-5 0.0 3.1695895786923396 0.0 78-79 1.4505466929167266E-4 7.252733464583633E-5 0.0 3.440479173594538 0.0 80-81 1.4505466929167266E-4 7.252733464583633E-5 0.0 3.7296003272433342 0.0 82-83 1.4505466929167266E-4 7.252733464583633E-5 0.0 4.0275244861347685 0.0 84-85 1.4505466929167266E-4 7.252733464583633E-5 0.0 4.326545620962721 0.0 86-87 1.4505466929167266E-4 7.252733464583633E-5 0.0 4.641441176161281 0.0 88 1.4505466929167266E-4 9.065916830729542E-5 0.0 4.893546191390207 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8550 0.0 57.131382 1 GTATCAA 15380 0.0 39.769146 1 ATCAACG 19120 0.0 31.510845 3 TCAACGC 19105 0.0 31.486387 4 CAACGCA 19760 0.0 30.395117 5 TATCAAC 20010 0.0 30.320688 2 AACGCAG 20370 0.0 29.538237 6 GTGGTAT 3740 0.0 26.021004 1 ACGCAGA 23565 0.0 25.373663 7 TGGTATC 3685 0.0 24.868925 2 CGCAGAG 23935 0.0 24.843838 8 GTACATG 14235 0.0 22.590399 1 GCAGAGT 26490 0.0 21.985676 9 TACATGG 14370 0.0 21.25771 2 GAGTACT 18620 0.0 20.631027 12-13 ACATGGG 14785 0.0 20.47031 3 AGAGTAC 24215 0.0 20.408575 10-11 CAGAGTA 25615 0.0 20.238968 10-11 AGTACTT 19385 0.0 18.907269 12-13 GTACTTT 20225 0.0 18.833042 14-15 >>END_MODULE