FastQCFastQC Report
Wed 25 May 2016
SRR2049362_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049362_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4328772
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42000.043.5376971
GTATCAA73500.037.9256021
TCAACGC76550.034.264874
ATCAACG78350.033.537673
CAACGCA81450.032.434365
AACGCAG86700.030.5763026
TATCAAC90300.029.4117452
ACGCAGA99800.026.1860057
CGCAGAG104200.024.8096128
GTACATG114650.024.723411
TACATGG117450.023.3332732
ACATGGG121750.021.6983873
GCAGAGT121400.020.326649
AGAGTAC102950.018.23960510-11
GAGTACA86600.018.238391
CATGGGG91900.017.8513074
GTGGTAT27450.017.4671861
CAGAGTA114850.017.31148510-11
GTCGTAC2859.585401E-716.4937821
AGTACAT92700.016.1252882
Produced by FastQC (version 0.11.5)